期刊
TOXINS
卷 12, 期 1, 页码 -出版社
MDPI
DOI: 10.3390/toxins12010048
关键词
EHEC; Stx2; stx(2); macrophages; intestinal epithelial cells; IL-1 beta; IL-8; mRNA
资金
- Agencia Nacional de Promocion Cientifica y Tecnologica of Argentina [PICT 2016-0278, PICT 2017-0046]
Enterohemorrhagic Escherichia coli (EHEC) strains are food-borne pathogens that can cause different clinical conditions. Shiga toxin 2a and/or 2c (Stx2)-producing E. coli O157:H7 is the serotype most frequently associated with severe human disease. In this work we analyzed the hypothesis that host cells participate in Stx2 production, cell damage, and inflammation during EHEC infection. With this aim, macrophage-differentiated THP-1 cells and the intestinal epithelial cell line HCT-8 were incubated with E. coli O157:H7. A time course analysis of cellular and bacterial survival, Stx2 production, stx(2) transcription, and cytokine secretion were analyzed in both human cell lines. We demonstrated that macrophages are able to internalize and kill EHEC. Simultaneously, Stx2 produced by internalized bacteria played a major role in macrophage death. In contrast, HCT-8 cells were completely resistant to EHEC infection. Besides, macrophages and HCT-8 infected cells produce IL-1 beta and IL-8 inflammatory cytokines, respectively. At the same time, bacterial stx(2)-specific transcripts were detected only in macrophages after EHEC infection. The interplay between bacteria and host cells led to Stx production, triggering of inflammatory response and cell damage, all of which could contribute to a severe outcome after EHEC infections.
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