Article
Cell Biology
Maria Serena Piccinno, Tiziana Petrachi, Marco Pignatti, Alba Murgia, Giulia Grisendi, Olivia Candini, Elisa Resca, Valentina Bergamini, Francesco Ganzerli, Alberto Portone, Ilenia Mastrolia, Chiara Chiavelli, Ilaria Castelli, Daniela Bernabei, Mara Tagliazucchi, Elisa Bonetti, Francesca Lolli, Giorgio De Santis, Massimo Dominici, Elena Veronesi
Summary: This study investigates the use of adipose-derived mesenchymal stromal/stem cells (AD-MSCs) in autologous fat transfer (AFT) for tissue reconstruction. The results show that AD-MSCs can improve graft persistence and reduce tissue resorption after AFT. The implanted AD-MSCs can persist and differentiate into mature adipocytes, leading to better outcomes in terms of graft weight and reduced cyst formation. This suggests a long-term protective and anti-inflammatory/proangiogenic role of AD-MSCs in autologous AFT tissue resorption.
Article
Medicine, Research & Experimental
L. B. Buravkova, M. Ezdakova, I. Andrianova, A. N. Gornostaeva, P. Bobyleva, E. R. Andreeva
Summary: The study demonstrated that during ex vivo expansion, cord blood hematopoietic stem progenitor cells (cbHSPCs) can influence the functional state of mesenchymal stromal cells (MSCs), leading to the formation of early stromal progenitors with a bipotential osteo-adipogenic profile. The joint coculture resulted in a specific hematopoiesis-supportive environment, with the expression of certain MSC genes upregulated and a balance of myeloid regulators maintained. Short-term osteoinduction prior to coculture enhanced the development of a bipotential profile, indicating a more committed state of cbHSPCs.
Article
Biotechnology & Applied Microbiology
Corina A. Ghebes, Jess Morhayim, Marion Kleijer, Merve Koroglu, Stefan J. Erkeland, Remco Hoogenboezem, Eric Bindels, Floris P. J. van Alphen, Maartje van den Biggelaar, Martijn A. Nolte, Bram C. J. van der Eerden, Eric Braakman, Carlijn Voermans, Jeroen van de Peppel
Summary: Recent research has shown that extracellular vesicles (EVs) secreted by adult bone marrow-derived mesenchymal stromal cells have the ability to support the ex vivo expansion of hematopoietic stem and progenitor cells (HSPCs), while those from fetal sources have a weaker effect. Protein composition analysis of EVs revealed that adult-derived EVs are rich in proteins related to oxidation-reduction processes, mitochondrial ATP synthesis, and protein folding, while fetal-derived EVs are rich in proteins linked to extracellular matrix organization, cell migration, and TGFBR signaling pathway.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Article
Cell Biology
Erica Ferrini, Fabio Franco Stellari, Valentina Franceschi, Francesca Macchi, Luca Russo, Alba Murgia, Giulia Grisendi, Gino Villetti, Massimo Dominici, Gaetano Donofrio
Summary: Mesenchymal stromal/stem cells (MSCs) have high regenerative potential and can guide tissue regeneration through their distribution in the lungs, with a longer persistence than previously observed. In vivo tracking techniques allow for the detection and monitoring of MSC clones in the body, potentially impacting pharmacokinetics and pharmacodynamics of MSCs as a therapeutic tool.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Review
Cell Biology
Mohamad Mahjoor, Arshia Fakouri, Simin Farokhi, Hojjatollah Nazari, Hamed Afkhami, Fatemeh Heidari
Summary: The skin is essential for the innate and adaptive immune systems, but skin lesions can have undesirable consequences. Mesenchymal stromal cells (MSCs) are being increasingly used for skin wound treatment due to their advantages in promoting healing. The oxygen concentration has an impact on MSC biology, and expanding MSCs in a hypoxic environment can enhance their effectiveness.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2023)
Article
Agriculture, Dairy & Animal Science
Hanan Hendawy, Akiko Uemura, Danfu Ma, Ryosuke Namiki, Haney Samir, Mahmoud F. Ahmed, Ahmed Elfadadny, Hussein M. El-Husseiny, Cheng Chieh-Jen, Ryou Tanaka
Summary: The study addressed the ideal adipose tissue harvesting site for obtaining high-quality stromal vascular fraction (SVF) cells in middle-aged to old dogs. The peri-ovarian region was found to yield the highest viability and cellular yield of SVF cells with greater expression of adipose-derived mesenchymal stem cell markers, making it the preferred AT sampling site for canine regenerative therapies.
Article
Cell & Tissue Engineering
Anja Lena Thiebes, Franziska E. Uhl, Marie Hauser, Christian G. Cornelissen, Stefan Jockenhoevel, Daniel J. Weiss
Summary: The study evaluated the feasibility of spraying MSCs using endoscopic atomization devices and found high survival rates, as well as the ability to induce cell differentiation. This suggests that endoscopic atomization may have a potential role in pulmonary MSC-based cell therapies.
Article
Cell Biology
Wu Duan, Huajie Zou, Nan Zang, Dongxia Ma, Bo Yang, Lin Zhu
Summary: Bone marrow adipose tissue (MAT) can have both local and systemic effects on metabolic homeostasis. The effects of metformin, a first-line drug for type 2 diabetes, on MAT and bone marrow mesenchymal stem cell (BM-MSC) differentiation are conflicting. In vivo and in vitro experiments showed that metformin increased MAT in mice tibia and promoted both osteogenic and lipogenic gene expression in mouse bone marrow. However, metformin inhibited lipogenesis and promoted osteogenesis of MSC in vitro. The increased MAT may be attributed to the filling of adipose tissue after metformin-induced apoptosis of bone marrow stromal cells.
Review
Biochemistry & Molecular Biology
Katarzyna Czerwiec, Malgorzata Zawrzykraj, Milena Deptula, Aneta Skoniecka, Agata Tyminska, Jacek Zielinski, Adam Kosinski, Michal Pikula
Summary: Adipose-derived mesenchymal stromal cells (AD-MSCs) have gained significant attention due to their easy availability from fat tissue and their regenerative and immunomodulatory properties. These cells show great potential in stem cell-based therapies for wound healing, orthopedic, cardiovascular, and autoimmune diseases. Numerous clinical trials have provided evidence for the effectiveness of AD-MSCs. This article presents current knowledge on AD-MSCs, their applications in pre-clinical models and clinical studies, and highlights the potential for chemically or genetically modified AD-MSCs as the next generation of stem cells.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Multidisciplinary Sciences
Quanlei Wang, Yuwei Wang, Chongfei Chang, Feilong Ma, Dongxiu Peng, Shun Yang, Yanru An, Qiuting Deng, Qixiao Wang, Fei Gao, Fei Wang, Huiru Tang, Xufeng Qi, Xiaoming Jiang, Dongqing Cai, Guangqian Zhou
Summary: The study successfully reprogrammed human umbilical cord-derived mesenchymal stem/stromal cells (UMSCs) into induced pluripotent stem cells (iPSCs) and further differentiated them into induced MSCs (iMSCs). A comparison between iMSCs and UMSCs revealed similarities in cell proliferative state, differentiation capability, and immunomodulatory potential, but differences in Homeobox gene expression and neural differentiation potential. iMSCs also demonstrated enhanced immunosuppression capacity.
Article
Pharmacology & Pharmacy
Valentina Cocce, Mara Bonelli, Silvia La Monica, Roberta Alfieri, Luisa Doneda, Eleonora Martegani, Giulio Alessandri, Costanza Annamaria Lagrasta, Aldo Gianni, Valeria Sordi, Francesco Petrella, Leda Roncoroni, Francesca Paino, Augusto Pessina
Summary: Malignant pleural mesothelioma, an asbestos-related tumor, has poor response to chemotherapy. Adult mesenchymal stromal cells derived from bone marrow or adipose tissue can be a good model for cell-based therapy, and using Paclitaxel-loaded mesenchymal stromal cells inhibits mesothelioma cell proliferation more effectively than Paclitaxel alone. In vivo studies using a minimal amount of 106 mesenchymal stromal cells loaded with Paclitaxel showed the same efficacy as systemic administration of Paclitaxel, supporting the use of mesenchymal stromal cells as a drug delivery system for solid tumors.
BIOCHEMICAL PHARMACOLOGY
(2023)
Review
Medicine, Research & Experimental
Mohammad Rudiansyah, Amr A. El-Sehrawy, Irfan Ahmad, Ermias Mergia Terefe, Walid Kamal Abdelbasset, Dmitry Olegovich Bokov, Aleli Salazar, Jasur Alimdjanovich Rizaev, Fares Mohammed Saeed Muthanna, Mohammed Nader Shalaby
Summary: Osteoporosis is a common and serious bone problem, especially in the elderly. Current treatments focus on pain relief but not on bone tissue recovery. However, stem cell therapy and exosomes are considered effective methods for treating and improving osteoporosis.
Article
Biochemistry & Molecular Biology
Agnieszka Krawczenko, Aleksandra Klimczak
Summary: MSCs, known for their regenerative and immunomodulatory properties, are typically isolated from bone marrow and adipose tissue. This review focuses on MSCs derived from adipose tissue and their secretome in regeneration processes, with a special emphasis on their contribution to angiogenic processes. Various clinical trials have been registered to explore the application of AT-MSCs and their secretome in treating different medical conditions.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Cell & Tissue Engineering
Liudi Wang, Tianyun Gao, Yu Li, Yuanyuan Xie, Sheng Zeng, Chenxu Tai, Yirui Feng, Pingping Shen, Bin Wang
Summary: Long-term inhibition of inflammation through gene modified stem cell therapy is an effective strategy in preventing diet-induced obesity and obesity-related metabolic syndrome.
STEM CELL RESEARCH & THERAPY
(2022)
Article
Cell Biology
Nataliya Petinati, Irina Shipounova, Natalia Sats, Alena Dorofeeva, Alexandra Sadovskaya, Nikolay Kapranov, Yulia Tkachuk, Anatoliy Bondarenko, Margarita Muravskaya, Michail Kotsky, Irina Kaplanskaya, Tamara Vasilieva, Nina Drize
Summary: This article investigates the properties of BM-MSCs before and after implantation under the renal capsule in a mini pig model. It is found that the implants formed ectopic foci containing bones, ectopic hematopoiesis, bone marrow stromal cells, and muscle cells. The cells that migrated out from these implants meet the criteria for MSCs and are designated as IM-MSCs.
Article
Physiology
Siqin Zhaorigetu, Henry Bair, Jonathan Lu, Di Jin, Scott D. Olson, Matthew T. Harting
JOURNAL OF VASCULAR RESEARCH
(2018)
Article
Cell & Tissue Engineering
Matthew T. Harting, Amit K. Srivastava, Siqin Zhaorigetu, Henry Bair, Karthik S. Prabhakara, Naama E. Toledano Furman, Jody V. Vykoukal, Katherine A. Ruppert, Charles S. Cox, Scott D. Olson
Article
Immunology
Supinder S. Bedi, Benjamin M. Aertker, George P. Liao, Henry W. Caplan, Deepa Bhattarai, Fanni Mandy, Franciska Mandy, Luis G. Fernandez, Pamela Zelnick, Matthew B. Mitchell, Walter Schiffer, Margaret Johnson, Emma Denson, Karthik Prabhakara, Hasen Xue, Philippa Smith, Karen Uray, Scott D. Olson, Robert W. Mays, Charles S. Cox
JOURNAL OF NEUROINFLAMMATION
(2018)
Article
Biotechnology & Applied Microbiology
Karthik S. Prabhakara, Daniel J. Kota, Gregory H. Jones, Amit K. Srivastava, Charles S. Cox, Scott D. Olson
Article
Multidisciplinary Sciences
Katherine A. Ruppert, Tin T. Nguyen, Karthik S. Prabhakara, Naama E. Toledano Furman, Amit K. Srivastava, Matthew T. Harting, Charles S. Cox, Scott D. Olson
SCIENTIFIC REPORTS
(2018)
Article
Cell & Tissue Engineering
Amit K. Srivastava, Karthik S. Prabhakara, Daniel Jkota, Supinder S. Bedi, Fabio Triolo, Katherine S. Brown, Matthew L. Skiles, Heather L. Brown, Charles S. Cox, Scott D. Olson
REGENERATIVE MEDICINE
(2019)
Review
Immunology
Henry Caplan, Scott D. Olson, Akshita Kumar, Mitchell George, Karthik S. Prabhakara, Pamela Wenzel, Supinder Bedi, Naama E. Toledano-Furman, Fabio Triolo, Julian Kamhieh-Milz, Guido Moll, Charles S. Cox
FRONTIERS IN IMMUNOLOGY
(2019)
Article
Neurosciences
Nikunj Satani, Kaavya Giridhar, Chunyan Cai, Natalia Wewior, Dominique D. Norris, Scott D. Olson, Jaroslaw Aronowski, Sean I. Savitz
Article
Cell & Tissue Engineering
Suchit Sahai, Marysuna Wilkerson, Hasen Xue, Nicolas Moreno, Louis Carrillo, Rene Flores, Matthew R. Greives, Scott D. Olson, Charles S. Cox, Fabio Triolo
TISSUE ENGINEERING PART A
(2020)
Article
Multidisciplinary Sciences
Katherine A. Ruppert, Karthik S. Prabhakara, Naama E. Toledano-Furman, Sanjna Udtha, Austin Q. Arceneaux, Hyeonggeun Park, An Dao, Charles S. Cox, Scott D. Olson
Article
Pediatrics
Madeline N. Monroe, Siqin Zhaorigetu, Vikas S. Gupta, Di Jin, Katelyn D. Givan, Alexander L. Curylo, Scott D. Olson, Charles S. Cox, Ana Segura, Louis Maximilian Buja, Kathryn Jane Grande-Allen, Matthew T. Harting
PEDIATRIC PULMONOLOGY
(2020)
Article
Multidisciplinary Sciences
Margaret L. Jackson, Katherine A. Ruppert, Daniel J. Kota, Karthik S. Prabhakara, Robert A. Hetz, Benjamin M. Aertker, Supinder Bedi, Robert W. Mays, Scott D. Olson, Charles S. Cox
Article
Biotechnology & Applied Microbiology
Yungang Xu, Weiling Zhao, Scott D. Olson, Karthik S. Prabhakara, Xiaobo Zhou
Article
Biochemical Research Methods
Naama E. Toledano Furman, Karthik S. Prabhakara, Supinder Bedi, Charles S. Cox, Scott D. Olson