Article
Immunology
Jennifer Roeder, Pascal Felgner, Michael Hensel
Summary: Salmonella enterica Typhimurium obtains nutrients required for rapid proliferation by forming Salmonella-induced filaments and expressing nutrient uptake systems, adapting uniquely to different host cell nutrient conditions. This leads to improved nutrient supply and bacterial proliferation.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2021)
Review
Immunology
Tatiana M. Clemente, Rajendra K. Angara, Stacey D. Gilk
Summary: Obligate intracellular pathogens can either reside freely in the host cell cytoplasm or be confined in a membrane-bound vacuole. The pathogens in membrane-bound vacuoles are protected from the innate immune system and antimicrobial drugs, but they face challenges such as obtaining membrane components, maintaining a suitable microenvironment, acquiring essential nutrients from the host cell, and manipulating the host cell through a specialized secretion system.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2023)
Article
Multidisciplinary Sciences
Marcel Hahn, Adriana Covarrubias-Pinto, Lina Herhaus, Shankha Satpathy, Kevin Klann, Keith B. Boyle, Christian Munch, Krishnaraj Rajalingam, Felix Randow, Chunaram Choudhary, Ivan Dikic
Summary: SIK2 kinase is a central component of the host defense machinery during Salmonella infection, and its depletion can lead to bacterial escape and impaired Xenophagy. During bacterial infection, SIK2 associates with actin and is recruited to the Salmonella-containing vacuole, controlling the formation of a protective SCV actin shield.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Biology
Dario Huesler, Pia Stauffer, Bernhard Keller, Desiree Boeck, Thomas Steiner, Anne Ostrzinski, Simone Vormittag, Bianca Striednig, A. Leoni Swart, Francois Letourneur, Sandra Maass, Doerte Becher, Wolfgang Eisenreich, Martin Pilhofer, Hubert Hilbi
Summary: This study investigates the interaction between Legionella pneumophila and lipid droplets (LDs) in the amoeba Dictyostelium discoideum. The researchers find that the fusion GTPase Sey1, the T4SS of L. pneumophila, and the Ran GTPase activator LegG1 all promote the interaction between LDs and the LCV. In addition, Sey1 and the L. pneumophila fatty acid transporter FadL are implicated in fatty acid metabolism and intracellular growth of the bacteria.
Article
Biochemistry & Molecular Biology
Felix Scharte, Rico Franzkoch, Michael Hensel
Summary: Salmonella enterica is a foodborne pathogen causing different diseases in humans. The mechanism of invasion and dissemination of typhoidal and non-typhoidal serovars are different. SPA exhibits cytosolic motility mediated by flagella, which allows it to evade xenophagy and contribute to the dissemination of systemic infection. This study used single-cell microscopy to analyze the triggers and cellular consequences of cytosolic motility.
MOLECULAR MICROBIOLOGY
(2023)
Review
Biochemistry & Molecular Biology
Dario Husler, Pia Stauffer, Hubert Hilbi
Summary: Lipid droplets (LDs) are versatile organelles involved in various cellular functions and disease pathogenesis. They are formed at the endoplasmic reticulum and play roles in lipid metabolism, energy storage, membrane trafficking, and cellular signaling. Several intracellular bacterial pathogens interact with LDs to obtain nutrients and establish their replicative niches. This review focuses on the biogenesis, interactions, and functions of LDs as well as their involvement in the lipid metabolism of intracellular bacterial pathogens.
MOLECULAR MICROBIOLOGY
(2023)
Article
Multidisciplinary Sciences
Ori Heyman, Dror Yehezkel, Camilla Ciolli Mattioli, Neta Blumberger, Gili Rosenberg, Aryeh Solomon, Dotan Hoffman, Noa Bossel Ben-Moshe, Roi Avraham
Summary: Encounters between host cells and intracellular bacterial pathogens lead to complex phenotypes that determine the outcome of infection. Single-cell RNA sequencing (scRNA-seq) has limited capacity to analyze the role of bacterial factors. The scPAIR-seq method developed in this study enables functional analysis of mutant-dependent changes in host transcriptomes, providing insights into bacterial virulence strategies and their interplay with host defense strategies.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Review
Immunology
Chak Hon Luk, Jost Enninga, Camila Valenzuela
Summary: Salmonella enterica exhibits distinct intracellular lifestyles within different cell types, which are determined by interactions between bacterial effectors and host factors. Understanding these differential interactions is crucial for elucidating the pathogen-host relationship during infection.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Cell Biology
Marc Schulte, Katharina Olschewski, Michael Hensel
Summary: Intracellular bacteria such as Salmonella enterica face various defense mechanisms from their mammalian host cells, and the ability to sense and respond to these damages is crucial for survival. By using fluorescence protein reporter strains, researchers were able to monitor stress responses and proliferation of single bacterial cells, leading to the detection and quantification of distinct subpopulations with varying levels of stress response and proliferation within S. enterica.
CELLULAR MICROBIOLOGY
(2021)
Review
Biochemistry & Molecular Biology
Naveen Challagundla, Deepti Phadnis, Aakriti Gupta, Reena Agrawal-Rajput
Summary: Lipids are complex organic molecules that play important roles in cell membranes. Bacteria can utilize host lipids for energy and manipulate host cells.
JOURNAL OF MEMBRANE BIOLOGY
(2023)
Article
Microbiology
Lin-lin Cai, Yun-ting Xie, Hai-jing Hu, Xing-lian Xu, Hu-hu Wang, Guang-hong Zhou
Summary: This study found that Salmonella adhesive-associated sRNA (SaaS) plays a critical role in the virulence phenotype of Salmonella Enteritidis. It is activated in the simulated intestinal environment and regulates the expression of virulence target genes. SaaS promotes invasion of epithelial cells while suppressing macrophage overgrowth and destruction. Its deletion significantly reduces mortality and attenuates bacterial dissemination and systemic inflammation.
MICROBIOLOGY SPECTRUM
(2023)
Article
Biochemistry & Molecular Biology
Xin Yang, Yan Chen, You Zhou, Chen Wu, Qing Li, Jun Wu, Wen Wei Hu, Wei Qing Zhao, Wei Wei, Chang Ping Wu, Jing Ting Jiang, Mei Ji
Summary: This study identified the mechanism of GPC5 in lung cancer, showing that it enhances the expression of CTDSP1 and miR-26b, inhibiting the proliferation of lung cancer cells and affecting the function of lymphatic endothelial cells. The findings suggest that GPC5 functions as a tumor suppressor in human lung cancer.
Article
Cell Biology
Dario Husler, Bernhard Steiner, Amanda Welin, Bianca Striednig, A. Leoni Swart, Virginie Molle, Hubert Hilbi, Francois Letourneur
Summary: The study reveals that D. discoideum Sey1 plays a crucial role in cell structure, proteolysis, cell motility, and intracellular bacterial replication, particularly in the context of Legionella pneumophila infection.
CELLULAR MICROBIOLOGY
(2021)
Article
Microbiology
Adam J. J. Svahn, Carl J. E. Suster, Sheryl L. L. Chang, Rebecca M. J. Rockett, Eby M. M. Sim, Oliver M. M. Cliff, Qinning Wang, Alicia Arnott, Marc Ramsperger, Tania C. C. Sorrell, Vitali Sintchenko, Mikhail Prokopenko
Summary: A major outbreak of Salmonella Enteritidis was identified in New South Wales, Australia through routine WGS analysis of clinical data. Pangenome analysis revealed the presence of a prophage called GF-1L, which carried the virulence factor gogB. This suggests that GF-1L could be an important marker for screening S. Enteritidis and that it may display similar virulence characteristics as Salmonella Typhimurium.
MICROBIOLOGY SPECTRUM
(2023)
Article
Engineering, Chemical
Huan Yang, Ella Hakkinen, Dian Ding, Runlong Cai, Juha Kangasluoma
Summary: This paper presents a new operation mode of the Electrical Mobility Spectrometer (EMS), called the Dynamic-aerosol-size Electrical Mobility Spectrometer (DEMS), which allows the aerosol particles to change their size during mobility classification in response to chemical/physical processes in the classification region. Unlike traditional EMS, DEMS focuses on probing the kinetics of aerosol-related processes rather than classifying the particle size. A theoretical framework is developed to analyze the transmission of size-changing particles in the classification region, and the validity of the framework is demonstrated through simulations and experiments.
JOURNAL OF AEROSOL SCIENCE
(2023)
Editorial Material
Microbiology
Rebecca Morrison, Chak Hon Luk, Maximiliano G. Gutierrez
Summary: In this study, it was discovered that Salmonella damages the Salmonella-containing vacuole using a type III secretion system, leading to complement deposition on intracellular bacteria and subsequent neutrophil efferocytosis that protects the bacteria from the respiratory burst.
CELL HOST & MICROBE
(2022)
Article
Biochemistry & Molecular Biology
Yang Si, Corentin Bon, Magdalena Barbachowska, Veronique Cadet-Daniel, Corinne Jallet, Laura Soresinetti, Mikael Boulle, Magalie Duchateau, Mariette Matondo, Fabrice Agou, Ludovic Halby, Paola B. Arimondo
Summary: Epigenetic regulation is a dynamic and reversible process that controls gene expression and plays a crucial role in human diseases like cancer. This study identified a compound that can inhibit cell proliferation and increase apoptosis by targeting specific histone marks, providing new insights into potential therapeutic targets and drug combinations.
RSC CHEMICAL BIOLOGY
(2022)
Article
Microbiology
Laura Piel, K. Shanmugha Rajan, Giovanni Bussotti, Hugo Varet, Rachel Legendre, Caroline Proux, Thibaut Douche, Quentin Giai-Gianetto, Thibault Chaze, Thomas Cokelaer, Barbora Vojtkova, Nadav Gordon-Bar, Tirza Doniger, Smadar Cohen-Chalamish, Praveenkumar Rengaraj, Celine Besse, Anne Boland, Jovana Sadlova, Jean-Francois Deleuze, Mariette Matondo, Ron Unger, Petr Volf, Shulamit Michaeli, Pascale Pescher, Gerald Spath
Summary: Genome instability plays a crucial role in the adaptation and regulation of the protozoan parasite Leishmania, utilizing post-transcriptional and epitranscriptomic regulation to adapt to its host environment.
Article
Multidisciplinary Sciences
Anna Griego, Thibaut Douche, Quentin Giai Gianetto, Mariette Matondo, Giulia Manina
Summary: RNA turnover is critical for gene expression variation and cellular adaptation. In this study, the role of RNase E in mycobacterial single-cell physiology and phenotypic diversification was explored. RNase E was found to form dynamic foci and interact with the nucleoid-associated protein HupB. Disruption of RNase E expression affected HupB levels and impaired the growth homeostasis of Mycobacterium tuberculosis.
Article
Multidisciplinary Sciences
Flore Nardella, Irina Dobrescu, Haitham Hassan, Fabien Rodrigues, Sabine Thiberge, Liliana Mancio-Silva, Ambre Tafit, Corinne Jallet, Veronique Cadet-Daniel, Stephane Goussin, Audrey Lorthiois, Yoann Menon, Nicolas Molinier, Dany Pechalrieu, Christophe Long, Francois Sautel, Mariette Matondo, Magalie Duchateau, Guillaume Medard, Benoit Witkowski, Artur Scherf, Ludovic Halby, Paola B. Arimondo
Summary: Malaria eradication requires the development of new drugs to combat drug-resistant parasites. Bisbenzylisoquinoline alkaloids isolated from Cocculus hirsutus were found to be active against Plasmodium falciparum blood stages. Compound 125, a derivative of these alkaloids, showed significantly improved preclinical properties and demonstrated effectiveness against drug-resistant clinical isolates and malaria transmission stages.
Article
Chemistry, Medicinal
Sandra Johannsen, Robin M. Gierse, Aleksandra Olshanova, Ellie Smerznak, Christian Laggner, Lea Eschweiler, Zahra Adeli, Rawia Hamid, Alaa Alhayek, Norbert Reiling, Joerg Haupenthal, Anna K. H. Hirsch
Summary: This work emphasizes the importance of investigating both on-target activity and antibiotic activity against critical pathogens. The authors focused on the 1-deoxy-d-xylulose 5-phosphate synthase (DXPS) as a potential new target and conducted virtual screening and activity studies. While no improvement in activity was observed in the synthesized derivatives, they found that the compounds showed good inhibition against Escherichia coli in their tests against various pathogens.
Review
Cell Biology
Ilana Berlin, Aysegul Sapmaz, Virginie Stevenin, Jacques Neefjes
Summary: The endolysosomal system is a dynamic network of vesicles that sense and interpret environmental cues, leading to homeostasis. To integrate extracellular signals with intracellular processes, endosomes and lysosomes make decisions on fusion or fission, recycling or degradation, and transport or contact with other organelles. These decisions are regulated by reversible post-translational modifications (PTMs) involving ubiquitin (Ub) and ubiquitin-like (Ubl) proteins. This ubiquitylation cycle controls protein stability and behavior on the endolysosomal membranes, fine-tuning macromolecular complex assembly and function. Additionally, this system is exploited by intracellular bacteria to hijack host trafficking pathways for their own benefit.
JOURNAL OF CELL SCIENCE
(2023)
Article
Immunology
Soraya Matczak, Valerie Bouchez, Pauline Leroux, Thibaut Douche, Nils Collinet, Annie Landier, Quentin Giai Gianetto, Sophie Guillot, Julia Chamot-Rooke, Milena Hasan, Mariette Matondo, Sylvain Brisse, Julie Toubiana
Summary: By comparing the microbiological characteristics and expressed protein profiles between FIM2 and FIM3 fimbrial serotypes and genomic clades, differences were found that may have implications on pathogenesis and epidemiological emergence.
MICROBES AND INFECTION
(2023)
Article
Multidisciplinary Sciences
Haibo Wang, Ligang Hu, Hongyan Li, Yau-Tsz Lai, Xueying Wei, Xiaohan Xu, Zhenkun Cao, Huiming Cao, Qianya Wan, Yuen-Yan Chang, Aimin Xu, Qunfang Zhou, Guibin Jiang, Ming-Liang He, Hongzhe Sun
Summary: In this study, the researchers identified the Cr(III)-proteome in cells and found that ATP synthase plays a crucial role in relieving hyperglycemia stress.
NATURE COMMUNICATIONS
(2023)
Article
Biotechnology & Applied Microbiology
Emile Auria, Lise Hunault, Patrick England, Marc Monot, Juliana Pipoli Da Fonseca, Mariette Matondo, Magalie Duchateau, Yannick D. N. Tremblay, Bruno Dupuy
Summary: The ability of Clostridioides difficile to form biofilms during infection and its association with recurrence and persistence are discussed in this study. The researchers investigate the role of the lipoprotein CD1687 in biofilm formation induced by deoxycholate (DCA). They found that CD1687 is necessary for biofilm formation, and its overexpression alone can induce biofilm formation. Furthermore, CD1687 affects the expression of transporters and metabolic pathways and binds DNA in a non-specific manner, suggesting its involvement in mediating the interaction between cells and the biofilm matrix.
NPJ BIOFILMS AND MICROBIOMES
(2023)
Article
Microbiology
Pierre Le-Bury, Karen Druart, Cyril Savin, Pierre Lechat, Guillem Mas Fiol, Mariette Matondo, Christophe Becavin, Olivier Dussurget, Javier Pizarro-Cerda
Summary: The genus Yersinia includes various nonpathogenic and pathogenic bacteria, causing a wide range of diseases. To analyze the omics data of Yersinia, we developed Yersiniomics, a web-based platform that provides easy access to genomic, transcriptomic, and proteomic data.
MICROBIOLOGY SPECTRUM
(2023)
Article
Multidisciplinary Sciences
Selma Metaane, Veronique Monteil, Thibaut Douche, Quentin Giai Gianetto, Mariette Matondo, Corinne Maufrais, Francoise Norel
Summary: In Salmonella bacteria, the absence of the magnesium transporter CorA affects the physiology and competitive ability of the cells. In the presence of high extracellular magnesium levels, the absence of CorA leads to the production of another magnesium transporter, MgtA, to compensate for it, and this process does not require PhoP. Moreover, the combination of Delta phoP and Delta corA mutations results in significantly reduced growth and motility, as well as decreased abundance of proteins involved in flagella formation, chemotaxis, and secretion.
Review
Microbiology
Ascel Samba-Louaka, Elisabeth Labruyere, Mariette Matondo, Marie Locard-Paulet, Jean-Christophe Olivo-Marin, Nancy Guillen
Summary: Amoebae, including pathogenic species and free-living species, are found in both aquatic and terrestrial environments and pose significant threats to public health. They exist in two cellular forms, trophozoites and cysts, with the cyst stage being the resistant and disseminating form. Encystation is accompanied by stress responses and limited data suggest that it shares factors and mechanisms with stress responses in trophozoites exposed to toxic compounds. Understanding the molecular mechanisms underlying amoebae-specific stress responses is crucial for the development of effective therapies.
Article
Multidisciplinary Sciences
Karla N. Valenzuela-Valderas, Elmira Farrashzadeh, Yuen-Yan Chang, Yunnuo Shi, Renee Raudonis, Brendan M. Leung, John R. Rohde, Jost Enninga, Zhenyu Cheng
Summary: This study reveals the importance of RACK1 in actin cytoskeleton dynamics and Shigella infection. RACK1 facilitates actin cytoskeleton remodeling during Shigella infection, leading to enhanced bacterial invasion and spreading. Silencing RACK1 expression impairs actin polymerization and reduces host cell susceptibility to Shigella infection.
Article
Biochemical Research Methods
Virginie Stevenin, Jacques Neefjes
Summary: The links between bacterial infections and cancer are actively being investigated. This study presents a cost-effective soft agar colony formation assay to quantify cell transformation after Salmonella Typhimurium infection. The protocol includes details on infection, seeding cells in soft agar, and automated cell colony enumeration. This assay can be adapted for studying other bacteria or host cells.