Article
Engineering, Environmental
Midori Yasui, Hikaru Iso, Shotaro Torii, Yasuhiro Matsui, Hiroyuki Katayama
Summary: Wastewater treatment for potable reuse is increasingly important globally, with advanced treatment processes like MF, UF and RO providing significant virus removal. Evaluating specific viruses like AiV, PMMoV, CGMMV, and crAssphage as process indicators, it was found that PMMoV and CGMMV are recommended indicators for physical virus removal in the overall water treatment process.
Article
Biotechnology & Applied Microbiology
Brienna L. Anderson-Coughlin, Shani Craighead, Alyssa Kelly, Samantha Gartley, Adam Vanore, Gordon Johnson, Chengsheng Jiang, Joseph Haymaker, Chanelle White, Derek Foust, Rico Duncan, Cheryl East, Eric T. Handy, Rhodel Bradshaw, Rianna Murray, Prachi Kulkarni, Mary Theresa Callahan, Sultana Solaiman, Walter Betancourt, Charles Gerba, Sarah Allard, Salina Parveen, Fawzy Hashem, Shirley A. Micallef, Amir Sapkota, Amy R. Sapkota, Manan Sharma, Kalmia E. Kniel
Summary: The study investigated human EVs and viral indicators in nontraditional irrigation water sources in the Mid-Atlantic region of the United States, finding a higher detection rate of EVs in reclaimed waters compared to surface waters. A significant correlation between EVs and PMMoV was observed in reclaimed water samples, but not in surface water samples. Salinity of the water significantly affected the detection of both EVs and PMMoV, as shown by logistic regression analyses.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2021)
Article
Multidisciplinary Sciences
Audrey Garcia, Tri Le, Paul Jankowski, Kadir Yanac, Qiuyan Yuan, Miguel Uyaguari-Diaz
Summary: This study investigated the potential use and quantification of human enteric viruses in municipal wastewater samples as indicators of contamination. The results suggest that these viruses can survive the wastewater treatment process and be released into the aquatic environment.
Article
Environmental Sciences
Ibrahim Ahmed Hamza, Sherif Abd-Elmaksoud
Summary: A major threat to water quality is the discharge of human-derived wastewater, which can cause waterborne illnesses associated with enteric viruses. A poor association exists between fecal indicator bacteria and virus fate in the environment, especially during wastewater treatment. This study evaluated the potential of using a novel human gut bacteriophage crAssphage as an indicator for the performance of viral reduction in the wastewater treatment process. The findings showed a significant correlation between crAssphage, HAdV, and HPyV during the wastewater treatment process, suggesting that crAssphage could be a reliable indicator for viral removal in wastewater treatment.
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2023)
Article
Engineering, Environmental
D. Shirakawa, N. Shirasaki, T. Matsushita, Y. Matsui, R. Yamashita, T. Matsumura, S. Koriki
Summary: We evaluated the reduction efficiencies of PMMoV and representative human enteric viruses in four full-scale drinking water treatment plants that use CS-RSF or C-MF. The PMMoV reduction ratios in C-MF were lower than those in CS-RSF, suggesting that CS-RSF has higher efficiency in removing PMMoV. The use of high-basicity PACl increased the reduction ratios of PMMoV in C-MF. PMMoV can be a potential surrogate for human enteric viruses to assess virus removal by CS-RSF.
Article
Agriculture, Multidisciplinary
Yue-yan Yin, Meng-ying Hua, Kuang-jie Zhao, Qiong-lian Wan, Shan Bu, Yu-wen Lu, Hong-ying Zheng, Shao-fei Rao, Fei Yan, Jie-jun Peng, Hai-ru Chen, Jian-ping Chen
Summary: This study describes a modified Cre/loxP recombination system that successfully alleviates necrosis induced by Cre recombinase in plants. The modified system can be used to construct chimera viruses and infectious clones for plant viruses with large genomes.
JOURNAL OF INTEGRATIVE AGRICULTURE
(2022)
Article
Environmental Sciences
Pradip Gyawali, Megan Devane, Paula Scholes, Joanne Hewitt
Summary: This study evaluated the source specificity, sensitivity, occurrence and concentration of three viral MST markers in water and shellfish, showing that crAssphage and PMMoV have higher predictive sensitivity for norovirus contamination in shellfish. The MST markers demonstrated high specificity and sensitivity in identifying faecal contamination sources in water and shellfish, with differences in predictive accuracy for norovirus contamination.
SCIENCE OF THE TOTAL ENVIRONMENT
(2021)
Article
Environmental Sciences
Akihiko Hata, Yuya Shirasaka, Masaru Ihara, Naoyuki Yamashita, Hiroaki Tanaka
Summary: Research on the occurrence and fate of enteric viruses in a Japanese lake was conducted over 14 months, with human enteric viruses not detected after April 2015, suggesting that virus inactivation may have been overestimated during the warm season.
SCIENCE OF THE TOTAL ENVIRONMENT
(2021)
Article
Microbiology
Peter J. Arts, J. Daniel Kelly, Claire M. Midgley, Khamal Anglin, Scott Lu, Glen R. Abedi, Raul Andino, Kevin M. Bakker, Bryon Banman, Alexandria B. Boehm, Melissa Briggs-Hagen, Andrew F. Brouwer, Michelle C. Davidson, Marisa C. Eisenberg, Miguel Garcia-Knight, Sterling Knight, Michael J. Peluso, Jesus Pineda-Ramirez, Ruth Diaz Sanchez, Sharon Saydah, Michel Tassetto, Jeffrey N. Martin, Krista R. Wigginton
Summary: This research is a critical step in the advancement of wastewater monitoring for public health. Previous studies lacked sufficient methodological information and relied on small-scale clinical reports or meta-analyses. The data presented here provide valuable longitudinal fecal shedding data for SARS-CoV-2, PMMoV, and crAssphage, enhancing the utility of wastewater-based epidemiology (WBE).
Article
Multidisciplinary Sciences
Balazs Kalapos, Csilla Juhasz, Eszter Balogh, Gabor Kocsy, Istvan Tobias, Gabor Gullner
Summary: The study found significant differences in the impact of two tobamoviruses on the transcriptome of pepper, with Obuda pepper virus markedly suppressing genes related to photosynthesis while PMMoV had minimal effect on disease signaling and defense pathways. This provides a deeper insight into the defense mechanisms underlying tobamovirus resistance in pepper.
SCIENTIFIC REPORTS
(2021)
Article
Engineering, Environmental
James P. R. Sorensen, Phil Aldous, Sarah Y. Bunting, Susan McNally, Barry R. Townsend, Megan J. Barnett, Tessa Harding, Roberto M. La Ragione, Marianne E. Stuart, Holly J. Tipper, Steve Pedley
Summary: The study investigated the seasonal prevalence of seven enteric viruses in groundwater-derived public water sources in England. The presence of viral NA showed seasonal patterns, with the highest concentration during the main groundwater recharge season. Coliforms were not significant predictors of viral NA presence, and there was limited co-occurrence between viruses. The study suggests the need for larger scale studies to manage potential groundwater viral contamination via risk assessment.
Article
Microbiology
Cheng Chen, Min Du, Deliang Peng, Wulun Li, Jingfeng Xu, Xiuling Yang, Xueping Zhou
Summary: A novel tobamovirus named Trichosanthes mottle mosaic virus (TrMMV) was discovered in Trichosanthes kirilowii plants, causing leaf mottling and mosaic symptoms. This virus was found to infect T. kirilowii and five other cucurbitaceous crops, highlighting its potential threat to agricultural production.
FRONTIERS IN MICROBIOLOGY
(2022)
Review
Virology
Miao Fang, Jisuk Yu, Kook-Hyung Kim
Summary: Studies have shown a possible relationship between symptom variations caused by PepMoV isolates and genetic variation. Researchers have identified several genes in pepper that confer resistance to PepMoV. However, the molecular mechanisms underlying the interaction between these resistance genes and PepMoV-encoded genes remain largely unknown.
Article
Environmental Sciences
Akihiko Hata, Yuno Meuchi, Miaomiao Liu, Shotaro Torii, Hiroyuki Katayama
Summary: Polymerase chain reaction (PCR) is widely used for monitoring pathogenic viruses in water environments. This study characterized and validated methods for quantifying active viruses and indicators in surface water. The study found that CDDP-RT-qPCR can be used for quantifying virus concentrations with higher detection frequencies than culture assays, but it may overestimate the activity of the virus.
SCIENCE OF THE TOTAL ENVIRONMENT
(2023)
Article
Plant Sciences
Miao Fang, Jisuk Yu, Hae-Ryun Kwak, Kook-Hyung Kim
Summary: This study investigates the symptom determinants and viral protein functions of Pepper mottle virus (PepMoV) in different isolates. The research identifies key regions involved in virus accumulation and symptom development, providing insights into the infection cycle and plant-virus interactions.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Environmental Sciences
Shotaro Torii, Wakana Oishi, Yifan Zhu, Ocean Thakali, Bikash Malla, Zaizhi Yu, Bo Zhao, Chisato Arakawa, Masaaki Kitajima, Akihiko Hata, Masaru Ihara, Shigeru Kyuwa, Daisuke Sano, Eiji Haramoto, Hiroyuki Katayama
Summary: This study compared five PEG precipitation procedures for virus concentration efficiency, finding that operational parameters significantly affected the recovery efficiency. Shorter incubation time outperformed overnight incubation, and PMMoV may be a potential indicator for evaluating SARS-CoV-2 recovery efficiency.
SCIENCE OF THE TOTAL ENVIRONMENT
(2022)
Editorial Material
Environmental Sciences
Eiji Haramoto, Gertjan Medema, John Scott Meschke
JOURNAL OF WATER AND HEALTH
(2022)
Article
Environmental Sciences
Sarmila Tandukar, Niva Sthapit, Ocean Thakali, Bikash Malla, Samendra P. Sherchan, Bijay Man Shakya, Laxman P. Shrestha, Jeevan B. Sherchand, Dev Raj Joshi, Bhupendra Lama, Eiji Haramoto
Summary: This study reports the presence and reduction of SARS-CoV-2 in wastewater from wastewater treatment plants and sewer lines in Nepal. It found a high prevalence of SARS-CoV-2 in wastewater, indicating that biological treatments at the treatment plants are not enough to eliminate the virus. The study suggests that wastewater-based epidemiology is a useful tool for estimating COVID-19 cases in developing countries.
SCIENCE OF THE TOTAL ENVIRONMENT
(2022)
Article
Engineering, Environmental
Guangming Jiang, Jiangping Wu, Jennifer Weidhaas, Xuan Li, Yan Chen, Jochen Mueller, Jiaying Li, Manish Kumar, Xu Zhou, Sudipti Arora, Eiji Haramoto, Samendra Sherchan, Gorka Orive, Unax Lertxundi, Ryo Honda, Masaaki Kitajima, Greg Jackson
Summary: Wastewater-based epidemiology (WBE) is a cost-effective and objective surveillance tool, but the correlation between viral concentrations in wastewater and clinical case numbers is weak. By developing artificial neural network (ANN) models based on wastewater data, accurate estimation of COVID-19 case numbers and transmission dynamics can be achieved.
Review
Environmental Sciences
Rajani Ghaju Shrestha, Yasuhiro Tanaka, Eiji Haramoto
Summary: Arcobacter is an emerging pathogen associated with human and animal diseases, capable of transmission through water and food. This review article provides an overview of the characteristics of Arcobacter, detection methods, and its global prevalence in various environmental and fecal samples.
Article
Engineering, Environmental
Muhammad Adnan Sabar, Ryo Honda, Eiji Haramoto
Summary: This article provides a comprehensive review on crAssphage in water, highlighting its high concentration and global distribution in wastewater and fecal-contaminated water bodies. It is highly specific to human-fecal contamination and has a potential as a performance indicator for virus reduction in wastewater treatment systems.
Article
Environmental Sciences
Made Sandhyana Angga, Bikash Malla, Sunayana Raya, Ayame Kitano, Xiaomao Xie, Hiroshi Saitoh, Noriyuki Ohnishi, Eiji Haramoto
Summary: The sensitivity of detecting SARS-CoV-2 in wastewater is crucial in wastewater-based epidemiology. In this study, a PEG-based method called Pegcision, using magnetic nanoparticles, was developed and modified to increase its detection sensitivity and throughput. The performance of the Pegcision was compared to that of the PEG precipitation method, and it showed comparable results in detecting SARS-CoV-2 RNA concentration. This suggests that the Pegcision can be a viable virus concentration method.
SCIENCE OF THE TOTAL ENVIRONMENT
(2022)
Article
Environmental Sciences
Ryo Iwamoto, Kiyoshi Yamaguchi, Chisato Arakawa, Hiroki Ando, Eiji Haramoto, Ken-ichi Setsukinai, Kotoe Katayama, Takuya Yamagishi, Sumire Sorano, Michio Murakami, Shigeru Kyuwa, Hiroyuki Kobayashi, Satoshi Okabe, Seiya Imoto, Masaaki Kitajima
Summary: This study investigated the presence and removal of SARS-CoV-2 in wastewater. It found that most SARS-CoV-2 particles in wastewater are associated with suspended solids, and that the removal efficiency of SARS-CoV-2 by the wastewater treatment system is comparable to or better than fecal indicators.
SCIENCE OF THE TOTAL ENVIRONMENT
(2022)
Article
Microbiology
Mohan Amarasiri, Tsubasa Takezawa, Bikash Malla, Takashi Furukawa, Jeevan B. Sherchand, Eiji Haramoto, Kazunari Sei
Summary: Antibiotic-resistant bacteria-associated infections cause over 1.2 million deaths annually worldwide. In low- and middle-income countries, the regulation of antibiotic consumption for human and veterinary use is ineffective, leading to the presence of antibiotics in aquatic environments and the dissemination of antibiotic resistance. Monitoring antibiotic resistance genes in aquatic environments is crucial for understanding the prevalence of antibiotic resistance determinants in these countries.
FRONTIERS IN MICROBIOLOGY
(2022)
Review
Engineering, Environmental
Xuan Li, Shuxin Zhang, Samendrdra Sherchan, Gorka Orive, Unax Lertxundi, Eiji Haramoto, Ryo Honda, Manish Kumar, Sudipti Arora, Masaaki Kitajima, Guangming Jiang
Summary: Wastewater-based epidemiology (WBE) is a promising approach for monitoring COVID-19, but the correlation between CRNA and clinically confirmed cases varies and the impacts of environmental and other factors are unclear. This study analyzed the correlation between CRNA and different types of clinically confirmed case numbers. It found a stronger correlation between CRNA and new cases, influenced by environmental and epidemiological conditions as well as sampling design.
JOURNAL OF HAZARDOUS MATERIALS
(2023)
Article
Environmental Sciences
Jatuwat Sangsanont, Surapong Rattanakul, Prasert Makkaew, Nopadol Precha, Pratchaya Rukthanapitak, Montakarn Sresung, Yadpiroon Siri, Masaaki Kitajima, Tomoko Takeda, Eiji Haramoto, Jiratchaya Puenpa, Nasamon Wanlapakorn, Yong Poovorawan, Skorn Mongkolsuk, Kwanrawee Sirikanchana
Summary: Wastewater-based epidemiology (WBE) can complement clinical surveillance of the distribution of SARS-CoV-2 and its variants in populations. Representative wastewater treatment plants in Bangkok and Phuket, Thailand, can serve as sentinel sites for early detection of new variants entering the country. WBE has the potential to support tourism-dependent economies and maintain public health protection through wastewater surveillance.
SCIENCE OF THE TOTAL ENVIRONMENT
(2023)
Article
Environmental Sciences
Bikash Malla, Ocean Thakali, Sadhana Shrestha, Takahiro Segawa, Masaaki Kitajima, Eiji Haramoto
Summary: This study applied high-throughput quantitative PCR (HT-qPCR) to detect SARS-CoV-2, its nucleotide substituted RNA, and other pathogenic viruses in wastewater. The results showed that using multiple assays increased the detection rate, and HT-qPCR may be the most time-efficient and cost-effective method for tracking COVID-19 and monitoring community health.
SCIENCE OF THE TOTAL ENVIRONMENT
(2022)
Review
Environmental Sciences
Ananda Tiwari, Sangeet Adhikari, Devrim Kaya, Md. Aminul Islam, Bikash Malla, Samendra P. Sherchan, Ahmad I. Al-Mustapha, Manish Kumar, Srijan Aggarwal, Prosun Bhattacharya, Kyle Bibby, Rolf U. Halden, Aaron Bivins, Eiji Haramoto, Sami Oikarinen, Annamari Heikinheimo, Tarja Pitkaenen
Summary: Monkeypox disease, caused by the monkeypox virus, has become a global concern. Wastewater-based surveillance can be a useful tool for early detection of the virus, but there are challenges to overcome.
SCIENCE OF THE TOTAL ENVIRONMENT
(2023)
Article
Environmental Sciences
Ryota Gomi, Eiji Haramoto, Hiroyuki Wada, Yoshinori Sugie, Chih-Yu Ma, Sunayana Raya, Bikash Malla, Fumitake Nishimura, Hiroaki Tanaka, Masaru Ihara
Summary: This study aimed to identify E. coli microbial source tracking (MST) markers that can be used to detect domestic wastewater contamination in environmental waters. Two MST markers, W_nqrC and W_clsA_2, were identified and showed higher performance than previously developed markers. PCR assays for these markers were also developed and validated.
SCIENCE OF THE TOTAL ENVIRONMENT
(2023)
Article
Environmental Sciences
Guangming Jiang, Yanchen Liu, Song Tang, Masaaki Kitajima, Eiji Haramoto, Sudipti Arora, Phil M. Choi, Greg Jackson, Patrick M. D'Aoust, Robert Delatolla, Shuxin Zhang, Ying Guo, Jiangping Wu, Yan Chen, Elipsha Sharma, Tanjila Alam Prosun, Jiawei Zhao, Manish Kumar, Ryo Honda, Warish Ahmed, Jon Meiman
Summary: Wastewater-based epidemiology (WBE) has the potential to track COVID-19 transmission, but faces methodological limitations. Future research should focus on standardizing sampling and analysis protocols, understanding viral stability in sewer systems, and utilizing artificial intelligence and big data models for smart wastewater surveillance. Further research is also needed for monitoring other viral pathogens and applying WBE beyond COVID-19.
CURRENT OPINION IN ENVIRONMENTAL SCIENCE & HEALTH
(2023)