Article
Environmental Sciences
Liping Ma, Huiying Yang, Lei Guan, Xiaoyu Liu, Tong Zhang
Summary: Chlorination process can significantly decrease both abundance and diversity of total ARGs, but with limited removal rates for opportunistic pathogens, such as E. coli isolates. Core subtypes of ARGs were identified and found to persist during chlorination for both total microbes and E. coli. Additionally, metagenomic binning strategies revealed enrichment of certain ARG-carrying genomes after chlorination.
ENVIRONMENT INTERNATIONAL
(2022)
Article
Environmental Sciences
Xiang Xiao, En-Jing He, Xue-Rong Lu, Li-Jun Wu, Yang-Yang Fan, Han-Qing Yu
Summary: The study shows that silver nanoparticles possess strong antibacterial ability, leading to the majority of bacteria entering a viable but non-culturable state. Environmental factors such as Cl- and illumination can affect the solubility of nanoparticles and thereby impact the survival state of bacteria.
SCIENCE OF THE TOTAL ENVIRONMENT
(2021)
Article
Cell Biology
Timothy Kudinha, Fanrong Kong
Summary: The prevalence of ST131, especially among ciprofloxacin resistant isolates, increased from fecal to clinical samples in women, men, and children. ST131 isolates showed higher virulence potential compared to non-ST131 isolates, particularly in urinary samples. There was a strong association between ST131 prevalence and fluoroquinolone resistance, with FQ resistant ST131 isolates harboring more virulence genes.
JOURNAL OF BIOMEDICAL SCIENCE
(2022)
Article
Environmental Sciences
Yuanzhen He, Hui Jin, Feng Ju
Summary: This study explores the toxicological effects and potential molecular mechanisms of chlorination-derived byproducts of the emerging contaminant metformin, namely 3,3-CDTA and NCDC. The byproducts exhibit stronger toxicity than metformin to Escherichia coli, disrupting cellular structures and components, decreasing membrane potential and ATP biosynthesis, and leading to excessive ROS and abnormal expression of ROS-scavenging enzymes. Proteomic analysis and molecular docking provide insights into the biomarker responses and indicate potential damage to DNA/RNA processes. This study broadens our understanding of the toxicity and transformation risks of metformin throughout its life process.
SCIENCE OF THE TOTAL ENVIRONMENT
(2023)
Article
Microbiology
Zuye Fang, Fubin Lai, Kun Cao, Ziyuan Zhang, Linlin Cao, Shiqin Liu, Yufeng Duan, Xingfeng Yin, Ruiguang Ge, Qing-Yu He, Xuesong Sun
Summary: Antibiotic resistance is a growing challenge to public health, and the regulation of acetylation in bacterial resistance to antibiotics remains unknown. In this study, the acetylated proteome of different antibiotic-resistant strains of Escherichia coli was analyzed. Protein acetylation was found to negatively regulate bacterial metabolism and positively regulate motility. Key enzymes in metabolic pathways were differentially acetylated, including pyruvate kinase (PykF), which was highly expressed and reversibly acetylated in resistant strains. Further analysis revealed that deacetylation of PykF increased enzymatic activity and sensitivity to antibiotics. This study provides new insights into bacterial resistance and the role of acetylation in antibiotic resistance regulation.
Article
Biochemical Research Methods
Noortje G. Godijk, Martin C. J. Bootsma, Henri C. van Werkhoven, Valentijn A. Schweitzer, Sabine C. de Greeff, Annelot F. Schoffelen, Marc J. M. Bonten
Summary: The study investigates whether infections with antibiotic-resistant bacteria replace or add to infections with antibiotic-susceptible bacteria. Using mathematical modeling and the example of plasmid-based beta-lactam resistant E. coli, the study finds that increased virulence is the only mechanism that increases the total number of infections, while other benefits of resistance lead to the replacement of susceptible infections.
PLOS COMPUTATIONAL BIOLOGY
(2022)
Article
Environmental Sciences
Zhenping Tang, Yu Zhang, Shasha Xiao, Yuanyuan Gao, Yi Duan, Boyang Liu, Cong Xiong, Zhengqing Yang, Yueyue Wu, Shuai Zhou
Summary: This study investigated the effects of a typical ketone on the variations of antibiotic resistance in E. coli. The results showed that low concentrations of the ketone inhibited the growth of resistant strains, but higher concentrations promoted the spread and expression of resistance genes while further inhibiting bacterial growth.
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2022)
Article
Multidisciplinary Sciences
Claudia Cobo-Angel, Steven M. Roche, Stephen J. LeBlanc
Summary: This study aimed to investigate the factors influencing dairy cattle veterinarians' prescribing of antimicrobials and their attitudes towards reducing antimicrobial use in the dairy industry. The study found that veterinarians have to consider various factors and face conflicts when making prescribing decisions. Younger veterinarians showed higher awareness of antimicrobial resistance but also experienced more pressure to comply with farmers' wishes.
Article
Microbiology
Timothy J. Dallman, Saskia Neuert, Cristina Fernandez Turienzo, Michelle Berin, Emily Richardson, Pablo Fuentes-Utrilla, Nicholas Loman, Saheer Gharbia, Claire Jenkins, Ron H. Behrens, Gauri Godbole, Michael Brown
Summary: International travel is a risk factor for acquisition of resistant organisms, as shown by increased abundance and diversity of antibiotic resistance genes in fecal samples post-travel. Resistance genes for macrolides, third-generation cephalosporins, aminoglycosides, and sulfonamides were particularly increased. The study also found a significant association between diarrhea or antibiotic use and increased resistome abundance.
MICROBIOLOGY SPECTRUM
(2023)
Article
Multidisciplinary Sciences
Mara Prentiss, Dianzhuo Wang, Jonathan Fu, Chantal Prevost, Veronica Godoy-Carter, Nancy Kleckner, Claudia Danilowicz
Summary: In E. coli, RecA protein is involved in resecting and loading double strand breaks (DSBs). It has been found that even though mismatches in homologous partners are rare, long lasting recombination products can still include many mismatches. This study shows that RecA forms observable recombination products with 16% mismatched bases in vitro.
Article
Biochemistry & Molecular Biology
Thi Thuy Nguyen, Le Minh Bui, Ji-Young Byun, Byung-Kwan Cho, Sun Chang Kim
Summary: This study explores the potential of a genome-reduced Escherichia coli as a host for pDNA production. By removing approximately 23% of the genome and increasing the abundance of NADPH, it was found that pDNA production capabilities were enhanced three-fold compared to the widely employed DH5 ff strain. Furthermore, the genome-reduced strain exhibited heightened sensitivity to various antibiotics, bolstering its potential for industrial pDNA production.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Microbiology
Severine Rangama, Ian D. E. A. Lidbury, Jennifer M. Holden, Chiara Borsetto, Andrew R. J. Murphy, Peter M. Hawkey, Elizabeth M. H. Wellington
Summary: In this study, strains of E. coli isolated downstream of a wastewater treatment plant in a river were found to carry multiple resistance genes, including bla(CTX-M-15) encoding an ESBL. E. coli strain 48 was shown to protect susceptible E. coli strain 33 from cefotaxime through secretion of a stable ESBL for at least 24 hours. CTX-M-15 was identified as the major secreted ESBL responsible for this protective effect, suggesting a public goods service for the survival of susceptible bacteria in the presence of cefotaxime.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2021)
Review
Microbiology
Muna F. Anjum, Heike Schmitt, Stefan Boerjesson, Thomas U. Berendonk, Eliana Guedes Stehling, Patrick Boerlin, Edward Topp, Claire Jardine, Xuewen Li, Bing Li, Monika Dolejska, Jean-Yves Madec, Christophe Dagot, Sebastian Guenther, Fiona Walsh, Laura Villa, Kees Veldman, Marianne Sunde, Pawel Krzeminski, Dariusz Wasyl, Magdalena Popowska, Josef Jaerhult, Stefan Oern, Olfa Mahjoub, Wejdene Mansour, Dinh Nho Thai, Josefine Elving, Karl Pedersen
Summary: The dynamics of antimicrobial resistance (AMR) are better understood through a One-Health perspective with surveillance playing a crucial role. Establishing environmental monitoring programs for AMR is urgently needed to complement existing systems in different sectors and to identify transmissions between human, animal, and environmental populations. Using antimicrobial resistant Escherichia coli as indicators for monitoring can help in assessing the occurrence and levels of AMR in the environment, including wildlife.
CURRENT OPINION IN MICROBIOLOGY
(2021)
Article
Chemistry, Multidisciplinary
Zheng Chen, Hailin Wang
Summary: Researching the impact of DNA methyltransferase on antibiotic toxicity can help address antibiotic resistance issues. Our experiments showed that E. coli strains lacking DNA methyltransferase genes were more sensitive to antibiotics.
Article
Multidisciplinary Sciences
An-Ni Zhang, Jeffry M. Gaston, Chengzhen L. Dai, Shijie Zhao, Mathilde Poyet, Mathieu Groussin, Xiaole Yin, Li-Guan Li, Mark C. M. van Loosdrecht, Edward Topp, Michael R. Gillings, William P. Hanage, James M. Tiedje, Katya Moniz, Eric J. Alm, Tong Zhang
Summary: Antibiotic resistance genes are common among bacteria, but not all pose high risks to human health. Researchers have developed an omics-based framework to rank these genes by risk, taking into account their enrichment in human associated environments, gene mobility, and host pathogenicity.
NATURE COMMUNICATIONS
(2021)
