Article
Cell Biology
Agoston G. Nagy, Inna Szekacs, Attila Bonyar, Robert Horvath
Summary: This study used high-throughput fluidic force microscopy to investigate the cellular adhesion maturation in epithelial cells. The results showed a significant strengthening adhesive tendency during the formation of Vero monolayers, but this step-by-step maturation was absent in HeLa cells. Additionally, cancerous HeLa cells displayed stronger adhesion to the tight Vero monolayer than cells of the same origin, and the mechanical characteristics of Vero monolayers upon cancerous HeLa cell influence were recorded and analyzed.
EUROPEAN JOURNAL OF CELL BIOLOGY
(2022)
Review
Cell Biology
Yuan Qiu, Chen-Chi Chien, Basile Maroulis, Jiani Bei, Angelo Gaitas, Bin Gong
Summary: This article presents a review of the applications of atomic force microscopy (AFM) and fluidic atomic force microscopy (fluidFM) in single-cell studies. It discusses the use of AFM in studying single cells, extracellular vesicles, colloidal force spectroscopy, and single-cell adhesion measurements. FluidFM, a combination of microfluidics and AFM, allows for biological, pathological, and pharmacological studies on single cells in a liquid environment. The review focuses on the capabilities of fluidFM in measuring adhesion, binding forces, and manipulating single cells, as well as its applications in patch clamping and mass measurements.
JOURNAL OF CELLULAR PHYSIOLOGY
(2022)
Article
Chemistry, Multidisciplinary
Livia Angeloni, Bogdan Popa, Mahdiyeh Nouri-Goushki, Michelle Minneboo, Amir A. Zadpoor, Murali K. Ghatkesar, Lidy E. Fratila-Apachitei
Summary: This study investigates the interaction between preosteoblasts and 3D-printed patterns using fluidic force microscopy and atomic force microscopy. The study reveals that taller pillar surfaces exhibit stronger cell adhesion strength and resistance to shear forces, which is crucial for the rational design of osteogenic patterns.
Article
Chemistry, Physical
Kinga Dora Kovacs, Martin Novak, Zoltan Hajnal, Csaba Hos, Balint Szabo, Inna Szekacs, Ye Fang, Attila Bonyar, Robert Horvath
Summary: Fluidic flow is crucial in colloid and interface sciences, and monitoring cell response to varied flow velocities using optical biosensors presents a challenging yet innovative approach. By rotating liquid over a biosensor array, the study demonstrated the capability to measure cell adhesion and detachment at different flow velocities simultaneously, providing insights into cellular behavior under mechanical and chemical stimulation. This setup offers a new avenue for investigating interface phenomena under controlled flow conditions.
JOURNAL OF COLLOID AND INTERFACE SCIENCE
(2021)
Article
Chemistry, Physical
Satoshi Ishii, Shogo Yoshimoto, Katsutoshi Hori
Summary: The research showed that Acinetobacter sp. Tol 5 has strong adhesion force, mainly achieved through the interaction between the AtaA protein on the cell surface and the surface. This adhesion force increases with the increase of AtaA molecules. Amino acid hydrolysates and deionized water can inhibit the direct interaction between cells and surfaces, respectively.
JOURNAL OF COLLOID AND INTERFACE SCIENCE
(2022)
Article
Chemistry, Analytical
Fangming Chen, Qian Guo, Qiaoyu Yang, Yao Meng, Mingrui Jiang, Jian Wang, De-Wen Zhang
Summary: Label-free electrochemical visualization of cancer cell apoptosis is achieved using a light-addressable electrochemical sensor. The study demonstrates that the AC photocurrent on apoptotic MCF-7 cells increases, indicating heterogeneity in apoptosis degrees. This new method provides potential applications in apoptosis-associated drug screening and efficacy evaluation.
ANALYTICAL CHEMISTRY
(2023)
Article
Physiology
Marie-Theres Wanuske, Dominique Brantschen, Camilla Schinner, Chiara Stuedle, Elias Walter, Matthias Hiermaier, Franziska Vielmuth, Jens Waschke, Volker Spindler
Summary: Dp is crucial for desmosome formation, while not influencing individual cadherin binding properties. Instead, macro-clustering of desmosomal adhesion molecules through Dp is crucial.
Article
Materials Science, Multidisciplinary
Franziska Weigl, Carina Blum, Ana Sancho, Juergen Groll
Summary: In recent decades, hybrid characterization systems have become important in cellular biomechanics research. This paper introduces a multifunctional device that combines Fluorescence Microscopy (FL) with FluidFM technology, allowing for real-time optical tracking of cell detachment processes while acquiring force-distance curves simultaneously.
ADVANCED MATERIALS TECHNOLOGIES
(2022)
Review
Biochemistry & Molecular Biology
Peter N. Lipke, Jason M. Rauceo, Albertus Viljoen
Summary: Mating and DNA transfer are crucial events for living organisms, and cell adhesion between gametes is essential for facilitating mating. Studies have provided insights into the structural information, specificities, and affinities of mating adhesins. Single-cell force spectroscopy has furthered our understanding of the design and evolution of these specialized adhesion proteins.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Chemistry, Multidisciplinary
Ping Zhou, Lurong Ding, Yajuan Yan, Yafeng Wang, Bin Su
Summary: Cell-matrix adhesions, mediated by integrins, play a crucial role in biological processes by connecting intracellular environments with the extracellular matrix. Advanced imaging techniques have facilitated the understanding of the molecular composition and dynamics of cell-matrix adhesions, including label-free imaging methods. This article provides an overview of recent developments in label-free imaging of cell-matrix adhesions, introducing electrochemiluminescence microscopy and surface plasmon resonance microscopy and discussing their applications. Furthermore, the advances in mapping cell-matrix adhesion force using molecular tension probes and fluorescence microscopy are highlighted.
CHEMICAL COMMUNICATIONS
(2023)
Review
Chemistry, Analytical
Zrinko Baricevic, Zahra Ayar, Samuel M. Leitao, Miranda Mladinic, Georg E. Fantner, Jelena Ban
Summary: Time-lapse light microscopy combined with in vitro neuronal cultures has made a significant contribution to Developmental Neuroscience. Although fluorescently labeled tags and dyes have provided advancements in cell imaging, long-term recordings remain challenging due to phototoxicity and interference with cell physiology. Hence, label-free high-resolution methods, such as scanning ion conductance microscopy (SICM) or digital holography microscopy (DHM) are proposed for studying live cell dynamics during neuronal development and regeneration.
Article
Materials Science, Paper & Wood
Claudia Gusenbauer, Karolina Peter, Etienne Cabane, Johannes Konnerth
Summary: Chemical force microscopy allows visualization of chemical nano-properties of materials and analysis of their surface behavior, making it valuable for studying natural substrates like wood. By studying adhesion forces and pK(a) values, this method provides insights into the complex chemical surface properties of materials.
Article
Chemistry, Physical
Rita Ungai-Salanki, Benjamin Csippa, Tamas Gerecsei, Beatrix Peter, Robert Horvath, Balint Szabo
Summary: This research investigated the physical background of biomolecular adhesion force through experiments and simulations, taking into account the impact of targeting offset on the results. The findings revealed that a higher targeting offset led to an increase in lifting force, while increasing flow rate actually decreased the lifting force. After reaching the maximum lifting force, further increasing the flow rate did not enhance the micropipette's manipulation force on the microbeads.
JOURNAL OF COLLOID AND INTERFACE SCIENCE
(2021)
Article
Nanoscience & Nanotechnology
Sebastian Sittl, Nicolas Helfricht, Georg Papastavrou
Summary: A different method based on hydrodynamic principles is proposed for the contactless calibration of (inverse) optical lever sensitivity (OLS) using the internal microfluidic channel of a FluidFM cantilever and external pressure control. This method allows for quantitative direct force measurements in complex environments.
Article
Chemistry, Physical
Zoltan Szittner, Beatrix Peter, Sandor Kurunczi, Inna Szekacs, Robert Horvath
Summary: This review summarizes the current state of label-free identification and functional characterization of leukocytes using biosensors and novel single cell techniques. Label-free characterization offers advantages such as decreased processing time and reagent costs, point-of-care diagnostics, and the ability to analyze cells in real-time kinetic processes. Techniques for characterizing single cells on solid substrates, particularly surface sensitive technologies, are emphasized. These techniques are important for studying small cell populations and individual cells in health and disease. Novel label-free approaches also provide solutions for extracting unlabeled cells for downstream processing.
ADVANCES IN COLLOID AND INTERFACE SCIENCE
(2022)
Article
Chemistry, Analytical
Eniko Farkas, Robert Tarr, Tamas Gerecsei, Andras Saftics, Kinga Dora Kovacs, Balazs Stercz, Judit Domokos, Beatrix Peter, Sandor Kurunczi, Inna Szekacs, Attila Bonyar, Anita Banyai, Peter Furjes, Szilvia Ruszkai-Szaniszlo, Mate Varga, Barnabas Szabo, Eszter Ostorhazi, Dora Szabo, Robert Horvath
Summary: There is a growing demand for bacteria repellent surfaces and antibody-based coatings in the field of biosensors. The optical waveguide lightmode spectroscopy (OWLS) technique offers a non-invasive and label-free method for characterizing protein and bacterial adsorption. In this study, an OWLS-based method was used to develop bacteria repellent surfaces and characterize different antibody-based coatings for bacterial assays. Nonspecific binding blocking agents were tested and immobilization methods for selected antibodies were applied. The performance of the biosensor was evaluated using enzyme-linked immunosorbent assay (ELISA) measurements. The best performance was achieved with a polyclonal antibody in combination with protein A-based immobilization and blocking of nonspecific binding.
Review
Chemistry, Analytical
Beatrix Peter, Eniko Farkas, Sandor Kurunczi, Zoltan Szittner, Szilvia Bosze, Jeremy J. Ramsden, Inna Szekacs, Robert Horvath
Summary: This article provides an overview and potential future directions of label-free biosensors in bacteria monitoring. While these sensors have the ability to detect bacteria adhesion, biofilm formation, and the effect of antibiotics, their application in basic biological research is still limited. The article discusses optical, mechanical, and electrical sensing technologies and summarizes key kinetic processes related to bacterial adhesion and survival, which could be explored further using label-free technologies. Additionally, the article explores the importance of antibacterial resistance, strategies to control adhesion, and bacteria-mammalian host cell interactions in the development and applications of biosensors.
Article
Biochemistry & Molecular Biology
Borbala Gemes, Eszter Takacs, Inna Szekacs, Robert Horvath, Andras Szekacs
Summary: The study demonstrates that glyphosate and its metabolites can inhibit multiple RGD binding integrins, affecting cell adhesion and physiological functions.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Chemistry, Analytical
Anita Banyai, Eniko Farkas, Hajnalka Jankovics, Inna Szekacs, Eszter Leelossyne Toth, Ferenc Vonderviszt, Robert Horvath, Mate Varga, Peter Furjes
Summary: The recent work aims to provide a better understanding of how Dean vortices impact lateral focusing and investigate how cell morphology influences the focusing position compared to spherical particles. Different flow rates and bead diameters were used to examine the position and extent of the focused region. The study generated a precise map of equilibrium positions for spherical beads, serving as a benchmark for focusing multi-dimensional particles and cells. Biological samples of various shapes, including rod-shaped E. coli, discoid biconcave-shaped RBCs, round or ovoid-shaped yeast, and soft-irregular-shaped cancer cells, were studied to explore the relationship between cell shape and the focusing position at the channel's end.
Article
Cell Biology
Andras Saftics, Sarah Abuelreich, Eugenia Romano, Ima Ghaeli, Nan Jiang, Michail Spanos, Kathleen M. Lennon, Gagandeep Singh, Saumya Das, Kendall Van Keuren-Jensen, Tijana Jovanovic-Talisman
Summary: This study developed a Single Extracellular VEsicle Nanoscopy (SEVEN) assay to comprehensively evaluate the quantity, size, shape, molecular tetraspanin content, and heterogeneity of extracellular vesicle subpopulations in human plasma. The assay successfully detected and characterized CD9-, CD63-, and CD81-enriched subpopulations, and also identified a unique pancreatic cancer-associated subpopulation. SEVEN could potentially be used as a platform for characterizing disease-associated and organ-associated extracellular vesicle subpopulations.
JOURNAL OF EXTRACELLULAR VESICLES
(2023)
Article
Cell Biology
Kinga Dora Kovacs, Tamas Visnovitz, Tamas Gerecsei, Beatrix Peter, Sandor Kurunczi, Anna Koncz, Krisztina Nemeth, Dorina Lenzinger, Krisztina V. Vukman, Anna Balogh, Imola Rajmon, Peter Lorincz, Inna Szekacs, Edit I. Buzas, Robert Horvath
Summary: Extracellular vesicles (EVs) have gained significant attention in biomedicine in the past decade. This report describes the direct nanoinjection of EVs into single cells using robotic fluidic force microscopy (robotic FluidFM). The study demonstrates the feasibility of nanoinjecting EVs and EV-like particles into various cell lines, providing valuable insights into cellular responses to EVs.
JOURNAL OF EXTRACELLULAR VESICLES
(2023)
Article
Materials Science, Multidisciplinary
Beatrix Peter, Nicolett Kanyo, Inna Szekacs, Antal Csampai, Szilvia Bosze, Robert Horvath
Summary: This study revealed the interaction between fibronectin and the natural active substance EGCG and its oxidized form, and investigated their effects on cancer cell adhesion. The results showed that both forms of polyphenols can bind to fibronectin and inhibit cellular adhesion, with the oxidized form having a more pronounced effect. The study also proposed a novel molecular scale active mechanism involving fibronectin's disulfide bridges to explain the observed kinetic signals and highlight the protein's role in adhesion.
MATERIALS ADVANCES
(2022)
Article
Chemistry, Physical
Tamas Gerecsei, Rita Ungai-Salanki, Andras Saftics, Imre Derenyi, Robert Horvath, Balint Szabo
Summary: This article describes the dissolution of water in oil, and derives simple equations to calculate the diffusion coefficient of water in oil. This is important for the production of miniature reactors for crystallization.
COLLOIDS AND INTERFACES
(2022)