Conditional control of RNA-guided nucleic acid cleavage and gene editing

Near-infrared upconversion–activated CRISPR-Cas9 system: A remote-controlled gene editing platform

(2019) Yongchun Pan et al.   Science Advances

RNA Cloaking by Reversible Acylation

(2018) Anastasia Kadina et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Optochemical Control of Biological Processes in Cells and Animals

(2018) Nicholas Ankenbruck et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Optimized RNP transfection for highly efficient CRISPR/Cas9-mediated gene knockout in primary T cells

(2018) Akiko Seki et al.   JOURNAL OF EXPERIMENTAL MEDICINE

Small Molecule Targeted Recruitment of a Nuclease to RNA

(2018) Matthew G. Costales et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

RNA Control by Photoreversible Acylation

(2018) Willem A. Velema et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Graphene oxide-mediated Cas9/sgRNA delivery for efficient genome editing

(2018) Huahua Yue et al.   Nanoscale

Incorporation of bridged nucleic acids into CRISPR RNAs improves Cas9 endonuclease specificity

(2018) Christopher R. Cromwell et al.   Nature Communications

Rapid and Efficient Genome Editing in Staphylococcus aureus by Using an Engineered CRISPR/Cas9 System

(2017) Weizhong Chen et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Enhanced proofreading governs CRISPR–Cas9 targeting accuracy

(2017) Janice S. Chen et al.   NATURE

Structure-guided chemical modification of guide RNA enables potent non-viral in vivo genome editing

(2017) Hao Yin et al.   NATURE BIOTECHNOLOGY

Human genetic variation alters CRISPR-Cas9 on- and off-targeting specificity at therapeutically implicated loci

(2017) Samuel Lessard et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

RNA editing with CRISPR-Cas13

(2017) David B. T. Cox et al.   SCIENCE

Nucleic acid detection with CRISPR-Cas13a/C2c2

(2017) Jonathan S. Gootenberg et al.   SCIENCE

Investigating Engineered Ribonucleoprotein Particles to Improve Oral RNAi Delivery in Crop Insect Pests

(2017) François-Xavier Gillet et al.   Frontiers in Physiology

Development of Light-Activated CRISPR Using Guide RNAs with Photocleavable Protectors

(2016) Piyush K. Jain et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

CRISPRs for Optimal Targeting: Delivery of CRISPR Components as DNA, RNA, and Protein into Cultured Cells and Single-Cell Embryos

(2016) Evguenia Kouranova et al.   HUMAN GENE THERAPY

Methods for Optimizing CRISPR-Cas9 Genome Editing Specificity

(2016) Josh Tycko et al.   MOLECULAR CELL

Two distinct RNase activities of CRISPR-C2c2 enable guide-RNA processing and RNA detection

(2016) Alexandra East-Seletsky et al.   NATURE

A chemical-inducible CRISPR–Cas9 system for rapid control of genome editing

(2016) Kaiwen Ivy Liu et al.   Nature Chemical Biology

Small-molecule control of protein function through Staudinger reduction

(2016) Ji Luo et al.   Nature Chemistry

C2c2 is a single-component programmable RNA-guided RNA-targeting CRISPR effector

(2016) Omar O. Abudayyeh et al.   SCIENCE

Structural roles of guide RNAs in the nuclease activity of Cas9 endonuclease

(2016) Youngbin Lim et al.   Nature Communications

The Development of a Viral Mediated CRISPR/Cas9 System with Doxycycline Dependent gRNA Expression for Inducible In vitro and In vivo Genome Editing

(2016) Christopher A. de Solis et al.   Frontiers in Molecular Neuroscience

A Covalent Approach for Site-Specific RNA Labeling in Mammalian Cells

(2015) Fahui Li et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Engineering Human Stem Cell Lines with Inducible Gene Knockout using CRISPR/Cas9

(2015) Yuejun Chen et al.   Cell Stem Cell

Rapid and highly efficient mammalian cell engineering via Cas9 protein transfection

(2015) Xiquan Liang et al.   JOURNAL OF BIOTECHNOLOGY

Optical Control of CRISPR/Cas9 Gene Editing

(2015) James Hemphill et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Discovery and Functional Characterization of Diverse Class 2 CRISPR-Cas Systems

(2015) Sergey Shmakov et al.   MOLECULAR CELL

Structural imprints in vivo decode RNA regulatory mechanisms

(2015) Robert C. Spitale et al.   NATURE

Chemically modified guide RNAs enhance CRISPR-Cas genome editing in human primary cells

(2015) Ayal Hendel et al.   NATURE BIOTECHNOLOGY

Inducible in vivo genome editing with CRISPR-Cas9

(2015) Lukas E Dow et al.   NATURE BIOTECHNOLOGY

A split-Cas9 architecture for inducible genome editing and transcription modulation

(2015) Bernd Zetsche et al.   NATURE BIOTECHNOLOGY

Photoactivatable CRISPR-Cas9 for optogenetic genome editing

(2015) Yuta Nihongaki et al.   NATURE BIOTECHNOLOGY

Small molecule–triggered Cas9 protein with improved genome-editing specificity

(2015) Kevin M Davis et al.   Nature Chemical Biology

A light-inducible CRISPR-Cas9 system for control of endogenous gene activation

(2015) Lauren R Polstein et al.   Nature Chemical Biology

Selective, rapid and optically switchable regulation of protein function in live mammalian cells

(2015) Yu-Hsuan Tsai et al.   Nature Chemistry

Development of an intein-mediated split–Cas9 system for gene therapy

(2015) Dong-Jiunn Jeffery Truong et al.   NUCLEIC ACIDS RESEARCH

CASFISH: CRISPR/Cas9-mediated in situ labeling of genomic loci in fixed cells

(2015) Wulan Deng et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Rationally engineered Cas9 nucleases with improved specificity

(2015) I. M. Slaymaker et al.   SCIENCE

An Inducible Lentiviral Guide RNA Platform Enables the Identification of Tumor-Essential Genes and Tumor-Promoting Mutations In Vivo

(2015) Brandon J. Aubrey et al.   Cell Reports

CRISPR/Cas9-mediated gene editing in human tripronuclear zygotes

(2015) Puping Liang et al.   Protein & Cell

Crystal Structure of Cas9 in Complex with Guide RNA and Target DNA

(2014) Hiroshi Nishimasu et al.   CELL

An iCRISPR Platform for Rapid, Multiplexable, and Inducible Genome Editing in Human Pluripotent Stem Cells

(2014) Federico González et al.   Cell Stem Cell

Highly efficient RNA-guided genome editing in human cells via delivery of purified Cas9 ribonucleoproteins

(2014) S. Kim et al.   GENOME RESEARCH

High-throughput screening of a CRISPR/Cas9 library for functional genomics in human cells

(2014) Yuexin Zhou et al.   NATURE

Palladium-triggered deprotection chemistry for protein activation in living cells

(2014) Jie Li et al.   Nature Chemistry

High-throughput profiling of off-target DNA cleavage reveals RNA-programmed Cas9 nuclease specificity

(2013) Vikram Pattanayak et al.   NATURE BIOTECHNOLOGY

High-frequency off-target mutagenesis induced by CRISPR-Cas nucleases in human cells

(2013) Yanfang Fu et al.   NATURE BIOTECHNOLOGY

Triphenylphosphinecarboxamide: An Effective Reagent for the Reduction of Azides and Its Application to Nucleic Acid Detection

(2013) Hisao Saneyoshi et al.   ORGANIC LETTERS

RNA-Guided Human Genome Engineering via Cas9

(2013) P. Mali et al.   SCIENCE

Multiplex Genome Engineering Using CRISPR/Cas Systems

(2013) L. Cong et al.   SCIENCE

Cas9-crRNA ribonucleoprotein complex mediates specific DNA cleavage for adaptive immunity in bacteria

(2012) G. Gasiunas et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

A Programmable Dual-RNA-Guided DNA Endonuclease in Adaptive Bacterial Immunity

(2012) M. Jinek et al.   SCIENCE

CRISPR RNA maturation by trans-encoded small RNA and host factor RNase III

(2011) Elitza Deltcheva et al.   NATURE

The Streptococcus thermophilus CRISPR/Cas system provides immunity in Escherichia coli

(2011) Rimantas Sapranauskas et al.   NUCLEIC ACIDS RESEARCH

The CRISPR/Cas bacterial immune system cleaves bacteriophage and plasmid DNA

(2010) Josiane E. Garneau et al.   NATURE