Article
Chemistry, Analytical
Shaohui Han, Hui Zhang, Xiuxiu Yue, Jingpei Wang, Lei Yang, Benhua Wang, Xiangzhi Song
Summary: A ratiometric fluorescent probe CP was developed to selectively and rapidly discriminate between Cys and Hcy by introducing a phenylethynyl group. With distinct ratiometric fluorescence changes induced by the presence of Cys or Hcy, the probe was able to visualize and discriminate between intracellular Cys and Hcy in HeLa cells and zebrafish.
ANALYTICAL CHEMISTRY
(2021)
Article
Chemistry, Analytical
Jingfeng Yao, Guoxing Yin, Ting Yu, Haitao Li, Peng Yin
Summary: A fluorescent probe has been developed that can simultaneously distinguish Cys/Hcy and GSH, and perform fluorescence imaging in live cells.
ANALYTICAL METHODS
(2021)
Article
Spectroscopy
Xiuxiu Yue, Jiali Chen, Wenqiang Chen, Benhua Wang, Hui Zhang, Xiangzhi Song
Summary: The endoplasmic reticulum, known as the largest organelle in many eukaryotic cells, plays a vital role in maintaining normal cell function. A novel endoplasmic reticulum-targeting fluorescent probe, ER-CP, has been developed for distinguishing and imaging Cys, Hcy, and GSH with favorable sensitivity and selectivity. ER-CP shows good ER-targeting property and has been successfully applied in imaging these biothiols in HeLa cells.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2021)
Review
Chemistry, Physical
S. Santhoshkumar, Manivannan Madhu, Wei-Bin Tseng, Wei-Lung Tseng
Summary: Gold nanoclusters (AuNCs) show great potential as ratiometric fluorescent probes due to their adjustable fluorescence wavelengths, biocompatibility, and resistance to photobleaching. By incorporating an additional fluorescence spectral peak, dual-emission AuNC-based probes have been developed to improve signal reproducibility. This review focuses on the preparation methods and applications of ratiometric fluorescent probes derived from AuNCs and other fluorescent nanomaterials or dyes for bioimaging. The challenges and future directions of using AuNCs for ratiometric bioimaging are also discussed.
PHYSICAL CHEMISTRY CHEMICAL PHYSICS
(2023)
Article
Chemistry, Multidisciplinary
Junyan Ma, Yaoyu Xu, Wen Zhao, Beibei Wang, Chunhuan Zhang, Zhenxing Zhang
Summary: Researchers have developed a Tag-Sec fluorescent probe that can detect both thioredoxin reductase and selenocysteine with high sensitivity and selectivity, along with broad pH tolerance and good water solubility. This concept may serve as a useful guide for designing probes for detecting Sec-containing proteins in clinical diagnosis.
MATERIALS CHEMISTRY FRONTIERS
(2021)
Article
Chemistry, Analytical
Yong-Peng Yang, Fu-Jian Qi, Ya-Long Zheng, De-Chen Duan, Xia-Zhen Bao, Fang Dai, Shengxiang Zhang, Bo Zhou
Summary: This study developed a fast imaging probe for mitochondrial TrxR2 and visualized the increased activity of TrxR2 in cancer cells, as well as the decreased activity in Parkinson's disease and ischemic stroke.
ANALYTICAL CHEMISTRY
(2022)
Article
Spectroscopy
Shuwei Zhang, Wenyi Liao, Xuewen Wang, Xinyao Wang, Ting Wang, Yu Yuan, Gang Chen, Xiaodong Jia
Summary: A novel fluorescent probe based on indanone was developed for effective detection of Cys and Hcy. The probe showed weak fluorescence but exhibited obvious enhancement when reacted with Cys/Hcy. It demonstrated good anti-interference and low cytotoxicity, making it suitable for monitoring Cys and Hcy in living cells.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2022)
Article
Chemistry, Multidisciplinary
Hong-Min Lv, Zi-Ke Jiang, Jing Wang, Tao Wang, Xiao-Nan Zhang, Yi-Meng Hu, Yan-Yan Ma
Summary: The study synthesized an AIE + ICT ratiometric fluorescent probe that can distinguish the presence of Hcy and Cys, with higher sensitivity towards Hcy. The probe has low cytotoxicity, is cell-permeable, and can be used for ratiometric fluorescence imaging of Cys in living cells.
NEW JOURNAL OF CHEMISTRY
(2021)
Article
Chemistry, Applied
Shuai Gong, Ahui Qin, Yan Zhang, Mingxin Li, Xiaoyi Chen, Yueyin Liang, Xu Xu, Zhonglong Wang, Shifa Wang
Summary: A novel ratiometric fluorescent probe has been developed for the rapid and accurate detection of L-Cysteine in food samples, demonstrating its practical biological application in cells and animal experiments.
Article
Chemistry, Inorganic & Nuclear
Hong Wang, Changli Zhang, Zhiyong Jiang, Li Xu, Zhipeng Liu
Summary: In this study, phenothiazine-fused boron complexes were identified as promising scaffolds for ratiometric fluorescent hypochlorite probes. The synthesized complexes demonstrated ultrafast and ratiometric absorption/fluorescence changes for hypochlorite. Additionally, an endoplasmic reticulum-targeting probe was developed and showed excellent real-time imaging ability for both endogenous and exogenous hypochlorite in living cells.
DALTON TRANSACTIONS
(2023)
Article
Biochemistry & Molecular Biology
Wei Wang, Zihao Peng, Min Ji, Junqing Chen, Peng Wang
Summary: In this study, a fluorescent probe SQM-NBD was synthesized for selective detection of Cys/Hcy. The probe showed superior sensitivity to Cys/Hcy and was able to achieve fluorescence imaging of Cys/Hcy in HeLa cells, demonstrating its potential for Cys/Hcy identification under physiological and pathological conditions.
BIOORGANIC CHEMISTRY
(2022)
Article
Chemistry, Multidisciplinary
Mahsa Shahrashoob, Saman Hosseinkhani, Hanieh Jafary, Morteza Hosseini, Fatemeh Molaabasi
Summary: PheDH is an ideal protein scaffold for the green synthesis of efficient gold nanoclusters. These nanoclusters exhibit excellent stability, solubility, shift and photoluminescence, making them suitable for the selective detection of mercury, copper, cysteine and glutathione.
Article
Chemistry, Analytical
Ya-Nan Wei, Bo Lin, Yang Shu, Jian-Hua Wang
Summary: A novel fluorescent probe ANET was designed to target mitochondria for detecting Cys, showing advantages of ratiometric fluorescence, high selectivity, and a large Stokes shift. Imaging of Cys was confirmed in HepG2 cells using this probe.
Article
Chemistry, Multidisciplinary
Jinrong Zheng, Jianlong Li, Hongli Luo, Lingbin Sun, Mangmang Sang, Xiu Yu
Summary: Hyperhomocysteinemia, associated with atherosclerosis and vascular disease, is a known risk factor. Developing a specific probe for hyperhomocysteinemia can provide early warning for cardiovascular diseases. The Hcy-specific fluorescent probe (HSFP) designed in this study has excellent selectivity and anti-interference capability, automatically switching on and off in water solution, and sensitively reflecting changes in Hcy content in cells.
Review
Chemistry, Inorganic & Nuclear
Thavasilingam Nagendraraj, Sakthivel Vishnu Priya, Jamespandi Annaraj, Suresh Sagadevan
Summary: The primary biothiols, cysteine (Cys), glutathione (GSH), and homocysteine (Hcy), have crucial roles in various biological processes. Fluctuations in redox balance can lead to diseases due to disruptions in perfectly regulated intracellular and extracellular microenvironments. A study was conducted to develop copper-based fluorescence probes for detecting extracellular and intracellular targets such as cysteine (Cys) and glutathione (GSH) respectively. The fluorescence of the probes coordinated with Cu2+ is quenched by the binding of either Cys or GSH, resulting in retained Cu2+ ions.
COORDINATION CHEMISTRY REVIEWS
(2023)
Article
Chemistry, Analytical
Hongda Li
ANALYTICA CHIMICA ACTA
(2015)
Article
Chemistry, Organic
Hongda Li, Pengcheng Zhao, Ning Zou, Hui Wang, Kaixuan Sun
TETRAHEDRON LETTERS
(2017)
Article
Chemistry, Analytical
Hongda Li
ANALYTICA CHIMICA ACTA
(2018)
Article
Chemistry, Analytical
Hongda Li, Tie Chen, Longyi Jin, Yuhe Kan, Bingzhu Yin
ANALYTICA CHIMICA ACTA
(2014)
Article
Chemistry, Multidisciplinary
Hongda Li, Zhi Wen, Longyi Jin, Yuhe Kan, Bingzhu Yin
CHEMICAL COMMUNICATIONS
(2012)
Article
Chemistry, Inorganic & Nuclear
Xiaojing Wu, Hongda Li, Yuhe Kan, Bingzhu Yin
DALTON TRANSACTIONS
(2013)
Article
Chemistry, Inorganic & Nuclear
Hongda Li, Liangliang Li, Bingzhu Yin
INORGANIC CHEMISTRY COMMUNICATIONS
(2014)
Article
Chemistry, Organic
Ningjuan Zheng, Hongda Li, Guangyan Sun, Keli Zhong, Bingzhu Yin
ORGANIC & BIOMOLECULAR CHEMISTRY
(2013)
Article
Chemistry, Analytical
Hongda Li, Longyi Jin, Yuhe Kan, Bingzhu Yin
SENSORS AND ACTUATORS B-CHEMICAL
(2014)
Article
Chemistry, Physical
Yucun Liu, Ningjuan Zheng, Hongda Li, Bingzhu Yin
Article
Chemistry, Organic
Hongda Li, Bao Li, Long-Yi Jin, Yuhe Kan, Bingzhu Yin
Article
Chemistry, Organic
Xiuqiong Chen, Hongda Li, Longyi Jin, Bingzhu Yin
TETRAHEDRON LETTERS
(2014)
Article
Medicine, Legal
Ning He, Mingliang Gao, Dunpu Shen, Hongda Li, Zhengbo Han, Pengcheng Zhao
FORENSIC SCIENCE INTERNATIONAL
(2019)
Article
Spectroscopy
Hongda Li, Rulin Jia, Yan Wang
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2020)
Article
Biophysics
Reza Khodadadi, Manouchehr Eghbal, Hamideh Ofoghi, Alireza Balaei, Ali Tamayol, Karen Abrinia, Amir Sanati-Nezhad, Mohamadmahdi Samandari
Summary: This paper introduces an integrated portable centrifugal microfluidic system that automates cell and fluid manipulation for complete blood counting (CBC) analysis at the point-of-care (POC). The system utilizes a specially designed microfluidic disc for cell separation, solution metering and mixing, and cell counting, and is equipped with a custom script for automated quantification of cells. The proposed method shows a strong correlation with the gold standard hematology analyzer for various blood parameters. The portable system offers simplicity, affordability, and low power consumption, making it a potential solution for improving healthcare delivery in resource-limited settings and remote areas.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Nabil H. Bhuiyan, Joon S. Shim
Summary: Developing herd immunity is crucial for changing the course of the COVID-19 pandemic. An AI-driven point-of-care testing platform has been proposed for analyzing the body's response to SARS-CoV-2, and it has been successfully used for quantifying viral proteins and antibodies in blood samples. A data-receptive web-based dashboard system has also been developed for real-time analysis of herd immunity progress.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Alexiane Godain, Timothy M. Vogel, Pascal Fongarland, Naoufel Haddour
Summary: This study provides comprehensive insights into the intricate relationship between shear stress and electroactive biofilms in microbial fuel cells, highlighting the pivotal role of shear stress in influencing the growth kinetics, electrical performance, and physical structure of the biofilms. The study also emphasizes the complexities of extracellular electron transfer mechanisms and the need for complementary metaproteomic analyses.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Linjie Wang, Yixin Chen, Yang Ji, Shujun Zheng, Fei Wang, Caolong Li
Summary: A paper-based biosensor incorporating surface-enhanced Raman spectroscopy (SERS) and colorimetric detection has been developed for efficient quantification of intracellular glutathione (GSH). The biosensor exhibits excellent selectivity, stability, and precision, with low detection limits in both SERS and colorimetric modes. It has been successfully utilized for intracellular GSH detection and validated against a commercial GSH assay kit.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Quan Zhou, Jing Wang, Zhen Zhang, Alain Wuethrich, Richard J. Lobb, Matt Trau
Summary: This study presents a biosensor for sensitive detection of EMT-associated biomarkers on extracellular vesicles (EVs) surfaces during targeted therapy. Through longitudinal monitoring of patients, the biosensor shows its ability to identify the EMT-like phenotype switching on circulating EVs during the development of resistance.
BIOSENSORS & BIOELECTRONICS
(2024)
Review
Biophysics
Pratika Rai, Sabrina N. Hoba, Celine Buchmann, Robert J. Subirana-Slotos, Christian Kersten, Tanja Schirmeister, Kristina Endres, Bernd Bufe, Alexey Tarasov
Summary: Proteases have been proposed as potential biomarkers for various pathological conditions. The development of protease biosensors offers a more efficient way to investigate dysregulated proteases. This review article presents different optical and electrochemical detection methods for designing biosensors for all major protease families.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Chengxin Liu, Xu Shen, Li Yan, Runlian Qu, Yue Wang, Yaqin He, Zixuan Zhan, Piaopiao Chen, Feng Lin
Summary: In this study, a homogeneous rapid electrochemical aptasensor was developed to quantitatively detect CTCs in lung cancer patients. The aptasensor utilized a DNA nanosphere structure and a complementary aptamer to specifically detect mucin 1 as a marker for CTCs. The assay exhibited high specificity and sensitivity, and the results were consistent with other detection methods.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Danni Xue, Xing Dai, Jialong Zhao, Jiayao Zhang, Huan Liu, Kui Liu, Tao Xu, Chenjie Gu, Xingfei Zhou, Tao Jiang
Summary: In this study, a dual-enhancement SERS substrate based on Ti3C2TX and Ag nanocubes was fabricated for precise quantification of ritonavir and ibrutinib in serum. The formation of numerous electromagnetic hotspots between Ag nanocubes facilitated effective photo-induced charge transfer. The composite substrate showed excellent sensitivity, achieving low detection limits and high recoveries, making it promising for monitoring and identification of clinical blood drug concentration.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Tenghua Zhang, Zihui Xie, Xiaohe Zheng, Yuxin Liang, Yao Lu, Hankang Zhong, Feiyang Qian, Yuqing Zhu, Ruiting Sun, Yan Sheng, Jiaming Hu
Summary: This study reports a technology based on cationic lipid-polymer hybrid nanoparticles for efficient extracellular vesicle (EV) enrichment and in-situ detection of internal microRNAs. The technology demonstrates high EV enrichment efficiency and sensitive internal RNA detection, making it potentially useful for early pancreatic cancer diagnosis.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Wenjie Dai, Gaoxu Chen, Xiaoyan Wang, Shujun Zhen, Chengzhi Huang, Lei Zhan, Yuanfang Li
Summary: In this study, a novel metal organic gel (MOG) with dual electrochemiluminescence (ECL) properties was prepared by simple mixing. The MOG exhibited strong and stable anodic and cathodic ECL signals. By utilizing this MOG, an ECL resonance energy transfer (ECL-RET) biosensor was constructed for ultrasensitive detection of a specific gene. The study developed a straightforward technique for obtaining a single luminescent material with dual signals and expanded the analytical application of MOGs in the realm of ECL.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Lais Canniatti Brazaca, Amanda Hikari Imamura, Rodrigo Vieira Blasques, Jessica Rocha Camargo, Bruno Campos Janegitz, Emanuel Carrilho
Summary: The use of microfluidic paper-based analytical devices (muPADs) for medical diagnosis is a growing trend due to their low cost, easy use, simple manufacturing, and potential for application in low-resource settings. This review focuses on the advances in muPADs for medical diagnostics, discussing their use in detecting various biomarkers in common human biofluids. The challenges of biomarker detection in each sample are examined, along with innovative techniques to overcome these limitations. The commercialization difficulties of muPADs are also considered, along with future trends such as wearable devices and integrated platforms.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Minghong Jian, Xudong Sun, Hua Zhang, Xiaotong Li, Shasha Li, Zhenxin Wang
Summary: Matrix metalloproteinases (MMPs) are attractive biomarkers for cancer diagnosis and treatment, but their low abundance in biological samples, especially in the early stages of tumors, makes it challenging to precisely analyze MMP activities. In this study, a peptide microarray-based metal-enhanced fluorescence assay (PMMEFA) is proposed as a sensitive and specific method to simultaneously detect MMP-1, -2, -3, -7, -9, and -13 activities. The PMMEFA showed excellent sensitivity and was capable of detecting MMP activities in various matrices.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Kyung Ho Kim, Eunsu Ryu, Zinah Hilal Khaleel, Sung Eun Seo, Lina Kim, Yong Ho Kim, Hyun Gyu Park, Oh Seok Kwon
Summary: We have developed a novel strategy for discriminative detection of SARS-CoV-2 variants using the plasmonic photothermal effect of gold nanofilms and digital polymerase chain reaction (dPCR) technology. With this method, we were able to detect the delta and omicron variants with high sensitivity within 25 minutes from COVID-19 patients' clinical samples, making it a rapid and accurate point-of-care testing tool.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Zepeng Kang, Yuanming Wang, Haiyan Song, Xueli Wang, Job Zhang, Zhiguang Zhu
Summary: By designing a wearable and flexible lactic-acid/O2 EBFC with an air-breathing biocathode, the limitations of biocathode are effectively solved. The optimal performance conditions are determined through experiments, and the EBFC is successfully applied to power a low-power device.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Huayun Chen, Zhiheng You, Yuhui Hong, Xiao Wang, Mingming Zhao, Yushi Luan, Yibin Ying, Yixian Wang
Summary: This study developed a colorimetric sensor array using gas-responsive two-dimensional metal-organic framework (MOF) composites for highly sensitive detection of volatile organic compounds (VOCs). The dye/Zn-2(bim)(4) composites-based sensor arrays showed enhanced sensitivity and anti-interference properties. The findings demonstrate the potential use of dye/Zn-2(bim)(4) sensor arrays for early perception of plant diseases.
BIOSENSORS & BIOELECTRONICS
(2024)