Article
Engineering, Environmental
Sheng-Qiang Fan, Wan-Ru Wen, Guo-Jun Xie, Yang Lu, Bing-Feng Liu, De-Feng Xing, Jun Ma, Nan-Qi Ren
Summary: Granular sludge combined n-DAMO and Anammox (n-D/A) is an energy-efficient biotechnique for simultaneous nitrogen and dissolved methane removal from wastewater. However, the lack of knowledge about metabolic interactions between n-DAMO and Anammox in granular sludge hinders the development of this biotechnology. In this study, metagenomic and metatranscriptomic analyses were conducted to investigate the metabolic interactions between n-DAMO and Anammox in granular sludge. The results revealed population shifts of n-DAMO and Anammox communities under different conditions, providing insights into the microbial interactions and ecological niches of these microorganisms.
Article
Environmental Sciences
Rayaan Harb, Dilan Lacin, Irmak Subasi, Tuba H. Erguder
Summary: The recently discovered process of denitrifying anaerobic methane oxidation (DAMO) connects the carbon and nitrogen biogeochemical cycles by linking the anaerobic oxidation of methane to denitrification. The DAMO process shows potential for reducing the greenhouse effect through the assimilation of dissolved methane, and offers a new perspective on denitrification through methane oxidation rather than organic matter. The two main microorganisms responsible for DAMO, M. oxyfera and M. nitroreducens, exist together in nature through a syntrophic relationship and may coexist with anaerobic ammonium oxidation (Anammox) bacteria, presenting opportunities for improved nitrogen removal systems and wastewater treatment alternatives.
JOURNAL OF ENVIRONMENTAL MANAGEMENT
(2021)
Article
Agricultural Engineering
Lili Yang, Wenxuan Li, Hongjuan Zhu, Sanqiang Dong, Hao Mu, Kaiyao Hu, Te Wang, Jie Li
Summary: Sponge iron (SI) has promising potential for nitrogen removal in the Anammox process, as it enhances the expression of specific key genes and improves nitrogen removal efficiency.
BIORESOURCE TECHNOLOGY
(2023)
Article
Environmental Sciences
Ziyao Wei, Yingnan Lai, Wenjing Li, Xiaochun Cui, Dandan Zhou, Chaofan Zhang, Congli Chen, Yuanping Fang
Summary: This study investigates the effects of reclaimed water containing chlorite on soil microorganisms by simulating subsurface infiltration. It was found that chlorite enhances the conversion of nitrate nitrogen to nitrite nitrogen but inhibits further conversion. Chlorite also induces oxidative stress reactions in cells and inhibits the production of EPSs. Additionally, chlorite leads to the enrichment of efflux resistance genes in the microbial community. This study provides important information for the management and reuse of reclaimed water.
Article
Microbiology
Clara Duffner, Anja Wunderlich, Michael Schloter, Stefanie Schulz, Florian Einsiedl
Summary: Bioremediation of polluted groundwater, especially nitrate contaminated groundwater, is a challenging task due to high nitrate concentrations exceeding drinking water limits. Stimulating autotrophic denitrifiers with hydrogen injection may be a promising remediation strategy, but challenges like cost and potential accumulation of cytotoxic intermediates and greenhouse gases need to be addressed.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
G. Massimo, R. S. Khambata, T. Chapman, K. Birchall, C. Raimondi, A. Shabbir, Nicki Dyson, K. S. Rathod, C. Borghi, A. Ahluwalia
Summary: XDH mutations have varying impacts on the biochemical activity of the enzyme, leading to potential harmful or protective influences on health. Targeting enzyme activity to enhance the nitrite-reductase profile may provide novel therapeutic options, especially in cardiovascular disease.
Article
Agricultural Engineering
Lei He, Xuejie He, Xing Fan, Shuohui Shi, Tao Yang, Hongyuan Li, Jian Zhou
Summary: This study found that the use of S. oneidensis MR-1 in combination with denitrifying microbial community can significantly enhance the bio-denitrification process through interspecies electron transfer (IET). The nitrogen removal rate was increased by 74.74% and the accumulation of nitrite was greatly reduced. The results also indicated that mediated interspecies electron transfer (MIET) played a role in enhancing denitrification, as observed when S. oneidensis MR-1 was separated by a dialysis bag.
BIORESOURCE TECHNOLOGY
(2023)
Article
Agricultural Engineering
Sike Wang, Kento Ishii, Heng Yu, Xuchuan Shi, Barth F. Smets, Alejandro Palomo, Jiane Zuo
Summary: The study found that rapid temperature drops had impacts on the performance of the anammox process and the metabolism of core microbial populations, with the metabolism of AnAOB responding more significantly to low temperatures compared to heterotrophs.
BIORESOURCE TECHNOLOGY
(2021)
Article
Engineering, Environmental
Hafiz Adeel Ahmad, Shakeel Ahmad, Linjie Gao, Sherif Ismail, Zhibin Wang, Amro El-Baz, Shou-Qing Ni
Summary: This study establishes a stable connection between PD and anammox, achieving continuous nitrite generation and stable denitrification process under the influence of a low-strength magnetic field. The nitrogen removal efficiency is improved, indicating the potential of this technique for energy-efficient and lower carbon nitrogen removal from wastewater.
Review
Physiology
Barbora Piknova, Alan N. Schechter, Ji Won Park, Anni Vanhatalo, Andrew M. Jones
Summary: Nonenzymatic nitric oxide generation through the reduction of nitrate and nitrite ions, along with high levels of nitrate ions in skeletal muscle, has been recently described. Skeletal muscle nitrate storage is crucial for maintaining NO homeostasis in healthy aging, and nitrate supplementation may be beneficial for treating specific pathophysiologies and enhancing normal functions.
EXERCISE AND SPORT SCIENCES REVIEWS
(2022)
Article
Biotechnology & Applied Microbiology
Siqi Fan, Bin Ji, Hassimi Abu Hasan, Jie Fan, Shaodong Guo, Jian Wang, Julin Yuan
Summary: This study evaluated the feasibility of the Microalgal-bacterial granular sludge (MBGS) process for treating aquaculture wastewater. Results showed significant removal of various pollutants under non-aerated conditions, with microbial assimilation identified as a key contributor. The synergy between microalgae and bacteria resulted in effective removal of nitrate and nitrite, demonstrating the potential of MBGS as an environmentally friendly and efficient alternative in aquaculture wastewater treatment.
BIOPROCESS AND BIOSYSTEMS ENGINEERING
(2021)
Article
Environmental Sciences
Yabing Meng, Depeng Wang, Pandeng Wang, Zhong Yu, Shasha Yuan, Lichao Xia, Fangang Meng
Summary: Understanding the response behaviors of anammox community under nitrite stress is crucial for successful application of anammox-related processes. The study found that nitrogen removal rates decreased under nitrite stress but recovered after the stress was terminated. A strong community succession was observed, driven by initial nitrite stress, while the dissimilarity of community mainly resulted from increased beta diversity of rare subcommunity. The rare subcommunity with high functional redundancy played a key role in maintaining the functional stability and resilience of the anammox community.
SCIENCE OF THE TOTAL ENVIRONMENT
(2022)
Article
Green & Sustainable Science & Technology
Chao Wang, Sen Qiao, Jiti Zhou
Summary: This study found that anaerobic ammonium oxidation (anammox) bacteria can use nitrate (NO3-) as the sole substrate and decompose intracellular carbon source (glycogen) to support nitrogen metabolism. The transcription gene and protein expression analysis also revealed the activation of key functions and metabolism pathways in anammox bacteria. This research provides new insights into the complete nitrogen removal ability and viability of anammox bacteria in different natural ecosystems.
JOURNAL OF CLEANER PRODUCTION
(2023)
Article
Environmental Sciences
Rui Xiao, Wanlu Zhu, Yuanzhu Zheng, Shaoyi Xu, Huijie Lu
Summary: This study reveals the metabolic features and interactions between SMP assimilators in wastewater anammox communities, providing important insights for the enrichment of AnAOB and the efficient and stable operation of wastewater anammox processes.
ENVIRONMENT INTERNATIONAL
(2022)
Article
Agricultural Engineering
Tianqi Zhang, Qidong Yin, Yunhong Shi, Guangxue Wu
Summary: The presence of organic carbon can influence nitrogen removal in the anaerobic ammonia oxidation process. Under mixotrophic conditions with intermittent addition of acetate and propionate, specific bacteria showed enhanced abilities to utilize different carbon sources, contributing to stable operation of the anammox system.
BIORESOURCE TECHNOLOGY
(2021)
Article
Engineering, Environmental
Mei Zhi, Zhongbo Zhou, Caiyun Yang, Yucheng Chen, Yeyuan Xiao, Fangang Meng
Summary: This study investigates the effects of solid retention times (SRTs) on microbial communities and interactions in algal-bacterial partial nitrification systems. The results show that an SRT of 10 days is ideal for stable nitrite accumulation, while longer SRTs result in more nitrate production. SRTs are strongly associated with microbial diversity and the functional microorganisms present.
CHEMICAL ENGINEERING JOURNAL
(2023)
Article
Engineering, Environmental
Ronghua Xu, Yuanyuan Yao, Zhongbo Zhou, Yu-Xi Huang, Shanshan Zhao, Fangang Meng
Summary: A pretreatment tank called HFB-immobilization tank was developed to capture hydrolytic/fermentative bacteria (HFB) in sludge supernatant. Two parallel anaerobic membrane bioreactors (AnMBRs) were operated to compare the performance with and without the HFB-immobilization tank. The experimental AnMBR showed higher methane yields, non-fouling state for more than 100 days, and better filterability of sludge supernatant compared to the control AnMBR. The sequencing data revealed lower abundances of HFB but higher abundances of methanogens in the experimental AnMBR, indicating efficient niche separation by the HFB immobilization.
CHEMICAL ENGINEERING JOURNAL
(2023)
Article
Plant Sciences
Yanmin Zou, Zeyang Liu, Jiaojiao Bai, Yuanyuan Zhou, Dongping Lu
Summary: The imbalance in the mitochondrial proteome leads to mitochondrial proteotoxic stresses, and the mechanisms of UPRmt signaling in plants are less understood. This study reveals that the DNA intercalating agent EtBr triggers UPRmt and activates ABA signaling in Arabidopsis thaliana.
ENVIRONMENTAL AND EXPERIMENTAL BOTANY
(2023)
Article
Engineering, Environmental
Ling-Dong Shi, Tian-Yu Gao, Xiao-Wen Wei, James P. Shapleigh, He-Ping Zhao
ENVIRONMENTAL SCIENCE & TECHNOLOGY
(2023)
Article
Peripheral Vascular Disease
Fang-Fei Wei, Yuanyuan Zhou, Yuzhong Wu, Xuwei Chen, Jiangui He, Yugang Dong, Jan A. Staessen, Chen Liu
Summary: The study found that cumulative blood pressure exposure is associated with adverse outcomes in heart failure with preserved ejection fraction (HFpEF). Longitudinal blood pressure measurements may help refine risk stratification for patients with HFpEF.
HYPERTENSION RESEARCH
(2023)
Article
Plant Sciences
Xiaotong Liu, Yuanyuan Zhou, Kexin Chen, Zejun Xiao, Xuelian Liang, Dongping Lu
Summary: This study reveals that the phosphorylation status of CPK28 affects its degradation mediated by ATL31. CPK28 is a negative regulator of plant innate immunity, and it undergoes ubiquitination mediated by ATL31 and degradation by the proteasome. The phosphorylation status of CPK28 impacts its association with ATL31 as well as ATL31-mediated ubiquitination and degradation.
Article
Engineering, Environmental
Zijian Chen, Fangang Meng, Chuyuan Zhou, Xiaowei Wu, Chao Jin
Summary: Adaptation to substrate fluctuations is a life reality for microbes in global municipal wastewater treatment plants. However, there is still a lack of definite information on how influent changes with different alteration frequencies shape the stability of anammox consortia and their metabolic regulations.
Article
Chemistry, Applied
Yuan-Yuan Zhou, Ning-Yu Liu, Xiao-Juan Zhang, Yan-Ping Sun, Yan Liu, Qi-Feng Fu, Bing-You Yang, Hai-Xue Kuang
Summary: Chemical investigation of green walnut husks led to the discovery of two new diarylheptanoid compounds, Juglanin K (1) and Myricananin I (2), along with ten known compounds (3-13). Their structures were determined through extensive spectroscopic analyses. The isolated compounds were tested for antibacterial activity against Escherichia coli and Staphylococcus aureus. Results showed that compound 2 exhibited moderate antibacterial activity, with MIC values of 0.313 and 0.156 mg/mL, respectively. Compounds 1, 3-7, and 13 demonstrated weak antibacterial activity against Escherichia coli and Staphylococcus aureus, with MIC values ranging from 0.625 to 2.5 mg/mL.
NATURAL PRODUCT RESEARCH
(2023)
Correction
Engineering, Environmental
Yifan Wang, Xing Zheng, Dongfei Li, Fangang Meng, Jiayu Tian, Mian Wang, Li Li, Hua Wu, Yaozhong Zhang
PROCESS SAFETY AND ENVIRONMENTAL PROTECTION
(2023)
Article
Environmental Sciences
Shasha Yuan, Sixian Guo, Yongtao Tan, Mengdi Li, Yi Lu, Ronghua Xu, Ahmed Tawfik, Zanmin Zhou, Jincan Chen, Wanli Liu, Fangang Meng
Summary: The ecological processes of attached biofilms and suspended biomass were studied in a sequencing batch moving bed biofilm reactor. Attached biofilms played a crucial role in nitrification and denitrification activities, while the different ecological processes were observed between attached and suspended communities in the reactor.
SCIENCE OF THE TOTAL ENVIRONMENT
(2023)
Article
Engineering, Environmental
Ronghua Xu, Wentian Zhang, Yue Fu, Fuqiang Fan, Zanmin Zhou, Jincan Chen, Wanli Liu, Fangang Meng
Summary: This study reveals the positive role of influent species immigration (ISI) in mitigating membrane fouling in membrane bioreactors (MBRs) treating municipal wastewater. The results show that ISI can reduce the fouling rate and the sludge from the MBR-C (fed with raw municipal wastewater) has higher fouling index and biofilm formation potential than the MBR-E (fed with sterilized municipal wastewater). The presence of diverse and abundant populations responsible for floc-forming, hydrolysis/fermentation, and SMP degradation in the influent supports the increased floc size and lowered SMP concentrations in the MBR-C.
Article
Oncology
Mingqing Cui, Jiawei Peng, Yuanyuan Zhou, Xixi Wang, Daxiang Cui
Summary: GPT2 expression is increased in certain cancers, including breast cancer. This study demonstrated that GPT2 is upregulated in TNBC and exosomal GPT2 promotes metastasis of breast cancer cells by activating BTRC. These findings suggest that exosomal GPT2 may serve as a potential biomarker and treatment target for breast cancer patients.
Article
Agricultural Engineering
Long -Xing Jiang, Lu Guo, James P. Shapleigh, Yi Liu, Yan Huang, Jin-Shi Lian, Ling Xie, Liang-Wei Deng, Wen-Guo Wang, Lan Wang
Summary: A 160-day incubation was conducted to investigate the effect of glutaraldehyde on anammox bacteria. The results showed that the nitrogen removal efficiency significantly decreased to 11% in the GA reactor when the glutaraldehyde concentration reached 40 mg/L, which was only one-quarter of that in the CK reactor. Glutaraldehyde caused the dissociation of anammox bacteria from granules and led to the succession of denitrifier community. Metagenome analysis revealed the replacement of denitrifiers with different efflux pumps and the lack of NodT proteins in a specific strain.
BIORESOURCE TECHNOLOGY
(2023)
Article
Chemistry, Medicinal
Yan Liu, Leixin Zhuang, Jinyan Tan, Yuanyuan Zhou, Peng Jiang, Ruifeng Fan, Haixue Kuang, Bingyou Yang
Summary: A new sesquiterpenoid compound, dmetelin J (1), was isolated from the leaves of Datura metel L., along with four known compounds: apocynol A (2), blumenol B (3), megastigmane (4), and ( + )-dehydrovomifoliol (5). The structure of dmetelin J (1) was determined using spectroscopic techniques, including 1D and 2D NMR spectroscopy and HRESIMS. Compounds 2, 3, and 5 were isolated from the Solanaceae family for the first time. Anti-inflammatory activity evaluation was performed on all isolated compounds.
NATURAL PRODUCT COMMUNICATIONS
(2023)
Article
Multidisciplinary Sciences
Jiaojiao Bai, Yuanyuan Zhou, Jianhang Sun, Kexin Chen, Yufang Han, Ranran Wang, Yanmin Zou, Mingshuo Du, Dongping Lu
Summary: Plant innate immunity relies on the protein BIK1, which is regulated by the ubiquitin ligases RGLG1/2 and PUB25. RGLG1/2 promote BIK1 accumulation and immune signaling, while PUB25 mediates BIK1 degradation. The interplay between these ubiquitin ligases maintains BIK1 homeostasis and regulates immune responses.
NATURE COMMUNICATIONS
(2023)
Article
Engineering, Environmental
Rong-Gui Zhu, Chang-Gui Pan, Feng-Jiao Peng, Chao-Yang Zhou, Jun-Jie Hu, Kefu Yu
Summary: This comprehensive survey investigated the occurrence, bioaccumulation, and trophic magnification of parabens and their metabolite 4-HB in a marine food web. Results showed that parabens were the predominant pollutants in marine organisms, with significant bioaccumulation from sediments. The estimated trophic magnification factor indicated biomagnification for MeP and trophic dilution for 4-HB. Overall, the risks for humans consuming marine organisms were found to be low.
Article
Engineering, Environmental
Andres F. Torres-Franco, Deborah Leroy-Freitas, Cristina Martinez-Fraile, Elisa Rodriguez, Pedro A. Garcia-Encina, Raul Munoz
Summary: Anaerobic and microalgae-based technologies have emerged as sustainable alternatives for municipal wastewater treatment. However, the presence of viruses in the treated wastewater is a major concern for reuse applications. This study assessed the ability of these technologies to reduce viruses during secondary wastewater treatment. The results showed that all technologies were effective in reducing the concentration of viruses, with microalgae-based treatment exhibiting the highest potential for reducing the disinfection requirements of treated wastewater.
Article
Engineering, Environmental
Young Gwang Kim, Sae Yun Kwon, Spencer J. Washburn, Scott C. Brooks, Ji Won Yoon, Lucien Besnard
Summary: The study uses Hg isotope ratios to identify the sources and exposure pathways of mercury in bivalves, finding that dissolved Hg phases in the water column are the primary source and exposure pathway to bivalves. This provides new insights into using bivalves as bioindicators for sediment quality monitoring.
Article
Engineering, Environmental
Hui Geng, Ying Xu, Rui Liu, Dianhai Yang, Xiaohu Dai
Summary: This study investigates the effect of cation exchange resin (CER) on the sequential recovery of hydrogen and methane from anaerobic digestion (AD) and the corresponding mechanisms. The results show that CER can simultaneously enhance the production of hydrogen and methane by promoting the solubilisation, hydrolysis, and acidification of organic matter. Additionally, CER facilitates effective contact between bacteria and organic particulates and reduces the energy barrier for mass transfer during methane production. The study also reveals changes in the microbial community structure and metagenomics during the AD process.
Article
Engineering, Environmental
Xiaojing Lin, Zhan Jin, Shunfeng Jiang, Zhiquan Wang, Suqing Wu, Ke Bei, Min Zhao, Xiangyong Zheng
Summary: Dehumidification combined with addition of absorbent resin supplement (ARS) was used to achieve rapid evaporation of non-pretreated urine, resulting in high water evaporation efficiency and nutrient recovery.
Article
Engineering, Environmental
Yangli Che, Chaoran Lin, Shen Li, Jiao Liu, Longhai Zhu, Shilei Yu, Nan Wang, Haoshuai Li, Mutai Bao, Yang Zhou, Tonghao Si, Rui Bao
Summary: Hydrodynamic processes play a crucial role in the transmission of sediments, microbial assembly, and organic carbon redistribution in the ocean. Through experiments and analysis, we found that hydrodynamics shape the assembly of microbial communities and control the redistribution of different sourced organic carbon, thereby influencing microbial-mediated biogeochemical transformation.
Article
Engineering, Environmental
Chao Chen, Yu Yang, Nigel J. D. Graham, Zhenyu Li, Xingtao Yang, Zhining Wang, Nadia Farhat, Johannes S. Vrouwenvelder, Li -an Hou
Summary: The fouling of seawater reverse osmosis membranes is a persistent challenge in desalination. This study monitored the operational performance of a desalination plant for 7 years and the fouling development in different areas of membrane modules. The findings showed that operational performance declined over time and fouling mainly occurred at the feed side of the modules, with the highest microbial diversity. Keystone species like Chloroflexi and Planctomycetes played an important role in maintaining community structure and biofilm maturation. Polysaccharides, soluble microbial products, marine humic acid-like substances, and inorganic substances contributed to fouling. Overall, biofouling had a significant impact on membrane fouling after 7 years of operation.
Article
Engineering, Environmental
Dan Li, Jieyi Sun, Yibo Fu, Wentao Hong, Heli Wang, Qian Yang, Junhong Wu, Sen Yang, Jianhui Xu, Yunfei Zhang, Yirong Deng, Yin Zhong, Ping'an Peng
Summary: Sulfidation-oxidation treatment of magnetite (Fe3O4) enhances the production of dark center dot OH, which can efficiently degrade dissolved organic matter (DOM) and accelerate carbon cycling.
Article
Engineering, Environmental
Cheng Yu, Kaijun Wang, Kaiyuan Zhang, Ruiyang Liu, Pingping Zheng
Summary: This study implemented a microaerobic-aerobic configuration in a full-scale municipal wastewater treatment facility and investigated the effects on sludge characteristics, pollutant removal, microbial community, and granulation mechanisms. The results showed successful transition from flocculent-activated sludge to well-defined AGS after two months of operation. The primary pathways for pollutant removal were simultaneous nitrification, denitrification, and phosphorus removal. Moreover, the incorporation of internal separators induced shifts in the flow pattern, which promoted granulation.
Article
Engineering, Environmental
Zhe Zhang, Shaoyang Hu, Guangrong Sun, Wei Wang
Summary: Halogenated aromatic disinfection byproducts (DBPs), such as halogenated phenols, have garnered widespread attention due to their high toxicity and prevalence. This study reports on the analysis, occurrence, and cytotoxicity of a group of emerging halogenated aromatic DBPs, known as halogenated polyhydroxyphenols (HPPs), in drinking water.
Article
Engineering, Environmental
Shengyue Chen, Jinliang Huang, Peng Wang, Xi Tang, Zhenyu Zhang
Summary: Accurate prediction of river water quality is crucial for sustainable water management. This study introduces wavelet analysis and transfer learning techniques to assist LSTM modeling, proposing a newly coupled modeling approach that improves short-term prediction of river water quality.
Article
Engineering, Environmental
Bang Du, Xinmin Zhan, Piet N. L. Lens, Yifeng Zhang, Guangxue Wu
Summary: Efficient anaerobic digestion relies on the cooperation of different microorganisms with different metabolic pathways. This study investigated the effects of different operational modes and the addition of powdered activated carbon (PAC) on ethanol metabolic pathways. The results showed that the SBR mode and the presence of CO2 facilitated ethanol metabolism towards propionate production, while the CFR mode with extended solids retention time enriched Geobacter. Adjusting operational modes and PAC addition can modulate anaerobic ethanol metabolism and enrich Geobacter.
Article
Engineering, Environmental
Wanfa Wang, Si-Liang Li, Jun Zhong, Yuanbi Yi, Fujun Yue, Zenglei Han, Qixin Wu, Ding He, Cong-Qiang Liu
Summary: This study compares the carbon biogeochemical processes in karst and non-karst regions within large thermal stratified river-reservoir systems. The results demonstrate that karst reservoirs have a reduced potential for carbon emissions and highlight the importance of considering geologic settings to improve accuracy in regional and global CO2 emission estimates.
Article
Engineering, Environmental
Chunxia Jiang, Zelong Zhao, Dong Zhu, Xiong Pan, Yuyi Yang
Summary: This study analyzed the occurrence and distribution of antibiotic resistance genes (ARGs) in different environmental media of the Yangtze River using metagenomics. Core resistome dominated by multidrug resistance genes was found in all samples, while rare resistome dominated by various resistance genes was more prevalent in plasmids. Specific bacteria were identified as hosts for both core and rare resistomes, with high clinical concern ARGs found in the rare resistome. Particle-associated environment provided the most ideal conditions for resistome hosts. This study provided insights into the genetic locations of ARGs and the community assembly mechanisms of ARG hosts in freshwater environments.
Article
Engineering, Environmental
Yu Zhang, Yongtao He, Linchun Jia, Lei Xu, Zheng Wang, Yueling He, Ling Xiong, Xumeng Lin, Hong Chen, Gang Xue
Summary: By synergizing organic carbon source, thiosulfate, and zero-valent iron, efficient mixotrophic denitrification of oligotrophic secondary effluent can be achieved. Thiosulfate plays a vital role in promoting TN removal efficiency, while corrosion of Fe0 releases OH- to neutralize H+ from thiosulfate-driven denitrification, creating a suitable environment for denitrification. The coordination of thiosulfate and Fe0 maintains the dominance of Thiobacillus for denitrification.