Article
Immunology
Guangyu Wang, Weiguo Sui, Wen Xue, Junning Zhang, Xueli Yang, Chune Mo, Xiaoping Pan, Minglin Ou, Xianliang Hou
Summary: This study investigates the dynamic changes in the BCR-H (TCR-beta) CDR3 repertoire of kidney transplant patients. Specific gene subfamilies and sequence combinations were identified at different time points during the transplantation process.
CLINICAL IMMUNOLOGY
(2022)
Article
Immunology
Zifei Wang, Yu Zhong, Zefan Zhang, Kaiqian Zhou, Zhihao Huang, Hao Yu, Longqi Liu, Shiping Liu, Huanming Yang, Jian Zhou, Jia Fan, Liang Wu, Yunfan Sun
Summary: This study comprehensively analyzes the characteristics of T-cell receptor (TCR) repertoire in tumor-infiltrating and circulating T cells in hepatocellular carcinoma (HCC) patients. The study reveals that HCC patients have a unique profile of TCR repertoire in peripheral blood, and establishes a diagnostic model based on TCR sequence for the non-invasive diagnosis of HCC.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Immunology
Hai Yang, Jason Cham, Brian Patrick Neal, Zenghua Fan, Tao He, Li Zhang
Summary: T cells are important for adaptive immunity and play a role in anti-tumoral immunity and defense against infections, including respiratory viruses like SARS-CoV-2. A customized pipeline for Network Analysis of Immune Repertoire (NAIR) was developed to analyze T-cell receptor (TCR) sequences. Network analysis and statistical methods were used to characterize the repertoire and correlate it with clinical outcomes. Disease-specific/associated clusters and shared clusters were identified, and a new metric incorporating clonal generation probability and clonal abundance was used to identify disease-specific TCRs. The proposed approach revealed potential disease-specific TCRs responsible for the immune response to COVID-19.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Biochemical Research Methods
Chun-Miao Ji, Xiao-Feng Yang, Pan Qin, Bin Wang, Yao-Wei Huang
Summary: High-throughput sequencing was used to study the antibody repertoire in pigs, revealing that specific V gene segments were preferentially used under normal conditions, while PEDV-infected pigs showed different patterns of IGHV usage.
JOURNAL OF VIROLOGICAL METHODS
(2021)
Article
Medicine, General & Internal
Fansheng Guo, Xiaoye Yuan, Jinglin Cao, Xin Zhao, Yang Wang, Wenpeng Liu, Baowang Liu, Qiang Zeng
Summary: This study identified key pathways and genes associated with HCC relapse after liver transplantation through RNA-seq and immune repertoire analysis. The immune repertoire analysis also revealed differences in immune cell types between the normal and relapse groups. These findings provide a research direction for immunotherapy in patients with HCC relapse after liver transplantation.
INTERNATIONAL JOURNAL OF GENERAL MEDICINE
(2023)
Article
Oncology
Andreas Agathangelidis, Elisavet Vlachonikola, Frederic Davi, Anton W. Langerak, Anastasia Chatzidimitriou
Summary: Precision medicine aims to overcome the complexity of cancer through integrating information from different 'subsystems'. Immunogenetics, facilitated by high-throughput approaches, enables a comprehensive understanding of the immune repertoire and improves clinical decision making. This article highlights the clinical utility of immunogenetic analysis in lymphoid malignancies, including diagnosis, disease monitoring, and its potential for immunotherapy.
SEMINARS IN CANCER BIOLOGY
(2022)
Article
Immunology
Yi Bai, Dapeng Chen, Chuanliang Cheng, Zhongmin Li, Hao Chi, Yuliang Zhang, Xiaoyu Zhang, Shaohai Tang, Qiang Zhao, Bing Ang, Yamin Zhang
Summary: The study examined the tumor immune microenvironment in hepatocellular carcinoma (HCC) and discovered the specific distribution of regulatory T (Treg) cells in HCC. Immune checkpoints and metabolic pathways were found to be uniquely overexpressed in Treg cells. Additionally, two subsets of NK cells with different cytotoxic capacities and a unique state of memory B cells were identified in HCC.
FRONTIERS IN IMMUNOLOGY
(2022)
Review
Biotechnology & Applied Microbiology
Marcel Passon, Stefaan De Smedt, Hristo L. Svilenov
Summary: In contrast to other species, cattle possess exceptional antibodies with ultra-long complementarity-determining regions (u1CDRs) that can consist of 40-70 amino acids. The bovine u1CDR is folded into a stalk and a disulfiderich knob domain, which enable binding to different antigens. This article summarizes the current knowledge of the u1CDR structure, discusses approaches to discover u1CDRs against novel antigens, and outlines protein engineering approaches inspired by natural u1CDRs. Furthermore, it highlights the potential of using isolated bovine knobs, known as picobodies, as small antigen-binding domains derived from natural antibodies.
BIOTECHNOLOGY ADVANCES
(2023)
Article
Multidisciplinary Sciences
Peng Zhu, Yongyan Pei, Jian Yu, Wenbin Ding, Yun Yang, Fuchen Liu, Lei Liu, Jian Huang, Shengxian Yuan, Zongyan Wang, Fangming Gu, Zeya Pan, Jinzhong Chen, Jinrong Qiu, Huiying Liu
Summary: The study aimed to identify abnormal lncRNAs and diagnostic biomarkers in hepatocellular carcinoma progression using high-throughput sequencing, and explore the underlying mechanisms. Transcriptome sequencing was used to analyze RNA expression profiles and identify differentially expressed RNAs. Hub lncRNAs were screened using various analyses, and their effects on HepG2 cell proliferation, migration, and invasion were investigated. A total of 610 lncRNAs, 2,593 mRNAs, and 26 miRNAs were identified in hepatocellular carcinoma patients compared to the control group.
Article
Biotechnology & Applied Microbiology
Xue Lin, Yingying Hua, Shuanglin Gu, Li Lv, Xingyu Li, Pin Chen, Peng Dai, Yunyun Hu, Anna Liu, Jian Li
Summary: Genomic localized hypermutation regions were found in cancers, associated with cancer prognosis. To quickly identify and visualize these regions, a lightweight and simple toolkit called kataegis was developed.
Article
Biochemistry & Molecular Biology
Hao Xiong, Liyan Ji, Jin Yang, Jianji Wan, Mengmeng Song, Guangren Liu, Ling Yang, Xiuqin Dong
Summary: This study assessed the TCRβ repertoire diversity and composition of CD8+ T cells in blood of 9 nonsegmental vitiligo patients via high-throughput sequencing. Vitiligo patients showed low TCRβ diversity with highly expanded clones. Differential usage of TRBV, the TRBJ gene, and the TRBV/TRBJ combination were observed between vitiligo patients and healthy controls. A set of TRBV/TRBJ combinations could differentiate vitiligo patients from healthy controls. This study revealed distinct TCRβ repertoires of CD8+ T cells in vitiligo and may contribute to the exploration of immune biomarkers and therapeutic targets.
MOLECULAR IMMUNOLOGY
(2023)
Article
Biology
Rossella Fasano, Mahdi Abdoli Shadbad, Oronzo Brunetti, Antonella Argentiero, Angela Calabrese, Patrizia Nardulli, Roberto Calbi, Behzad Baradaran, Nicola Silvestris
Summary: Hepatocellular carcinoma (HCC) is the fourth leading cause of cancer-related death worldwide, with increased infiltration of immunosuppressive immune cells and elevated expression of immunosuppressive factors playing a key role in creating the immunosuppressive nature of the HCC environment. Immunotherapy, including immune checkpoint inhibitors (ICIs) and vaccines, has shown promise in treating HCC, although robust antitumoral responses have not been consistently induced in clinical settings.
Article
Medicine, General & Internal
Roberta Amoriello, Maria Chernigovskaya, Victor Greiff, Alberto Carnasciali, Luca Massacesi, Alessandro Barilaro, Anna M. Repice, Tiziana Biagioli, Alessandra Aldinucci, Paolo A. Muraro, David A. Laplaud, Andreas Lossius, Clara Ballerini
Summary: CSF repertoires in MS patients show a significantly higher percentage of public clones and sequence similarity compared to peripheral blood, but the repertoire polarization between CSF and PB is not significantly different. One PPMS CSF repertoire differs in TCR similarity architecture. Cluster analysis distinguishes MS from HD.
Article
Medicine, Research & Experimental
Hongmei Liu, Wenjing Pan, Congli Tang, Yujie Tang, Haijing Wu, Akihiko Yoshimura, Yan Deng, Nongyue He, Song Li
Summary: The adaptive immune response recognizes and neutralizes threats through T or B lymphatic receptors. High-throughput sequencing and bioinformatics offer research opportunities. Analyzing immune repertoire data reveals biological information and aids in improving experimental and computational methods.
Article
Biotechnology & Applied Microbiology
Pingsen Zhao, Sharula Guo, Zhixiong Zhong, Songtao Yang, Xianzhu Xia
Summary: The study revealed that rabies virus vaccination alters BCR diversity and gene segment usage, inducing B cell clone expansion. Quantitative characterization of human B cell receptor repertoire post rabies virus vaccination can provide new insights into the B cell receptor condition.
HUMAN VACCINES & IMMUNOTHERAPEUTICS
(2021)
Article
Chemistry, Medicinal
Hanyi Tan, Yue Liu, Chaochao Gong, Jiawei Zhang, Jian Huang, Qian Zhang
Summary: Focal adhesion kinase (FAK) is a key player in various cellular functions, and in this study, a novel 7H-pyrrolo[2,3-d]pyrimidine derivative, 16c, was found to effectively inhibit tumor growth by targeting FAK through downregulating the phosphorylation cascade. The compound showed promising bioactivity in vivo without affecting animal body weight, highlighting its potential as a therapeutic agent for hepatocellular carcinoma.
EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY
(2021)
Review
Geriatrics & Gerontology
Yiyan Sun, Xiaohuan Xia, Diksha Basnet, Jialin C. Zheng, Jian Huang, Jianhui Liu
Summary: Neurodegenerative diseases are characterized by the degeneration of neuronal cells and synaptic loss, with ferroptosis playing an important role in the pathogenesis.
FRONTIERS IN AGING NEUROSCIENCE
(2022)
Article
Oncology
Yan An, Yanfang Guan, Yaping Xu, Yingxin Han, Chi Wu, Chaohui Bao, Boping Zhou, Haiyan Wang, Mingxia Zhang, Weilong Liu, Lin Qiu, Zeguang Han, Yongsheng Chen, Xuefeng Xia, Jiayin Wang, Zhentian Liu, Wanqiu Huang, Xin Yi, Jian Huang
AMERICAN JOURNAL OF TRANSLATIONAL RESEARCH
(2019)
Review
Oncology
Xinru Zhou, Yin Jia, Chuanbin Mao, Shanrong Liu
Summary: Small extracellular vesicles (sEVs), such as exosomes, have emerged as crucial targets for liquid biopsy and promising drug delivery vehicles in tumor progression. They can serve as biomarkers for tumor diagnosis and as drug carriers for cancer treatment.
Article
Oncology
Ruochan Chen, Ju Zhu, Xiao Zhong, Jie Li, Rui Kang, Daolin Tang
Summary: The interplay between autophagy and apoptosis plays a crucial role in tumorigenesis and cancer therapy, with HMGB1 serving as a key regulator in these processes.
Article
Oncology
Zongfu Pan, Xixuan Lu, Tong Xu, Jinming Chen, Lisha Bao, Ying Li, Yingying Gong, Yulu Che, Xiaozhou Zou, Zhuo Tan, Ping Huang, Minghua Ge
Summary: This study uncovered the emerging role of HN1 in promoting dedifferentiation of anaplastic thyroid cancer (ATC) cells. HN1 negatively regulated the thyroid differentiation markers and had an inhibitory effect on the transcriptional activation of CTCF, thereby influencing the chromatin accessibility of thyroid differentiation genes.
Article
Oncology
Yi Qin, Shengjun Xiong, Jun Ren, Gautam Sethi
Summary: Autophagy plays an important regulatory role in glioblastoma, and its dysregulation can lead to drug resistance and radioresistance. It also affects stem cell characteristics, overall growth, and metastasis. Therefore, autophagy is a promising target for glioblastoma therapy.
Article
Oncology
Katsuya Nagaoka, Xuewei Bai, Dan Liu, Kevin Cao, Joud Mulla, Chengcheng Ji, Hongze Chen, Muhammad Azhar Nisar, Amalia Bay, William Mueller, Grace Hildebrand, Jin-Song Gao, Shaolei Lu, Hiroko Setoyama, Yasuhito Tanaka, Jack R. Wands, Chiung-Kuei Huang
Summary: This study found that serum 2-OG levels in cholangiocarcinoma patients are associated with the effectiveness of chemotherapy. Patients with progressive disease showed significantly higher levels of serum 2-OG compared to stable disease and partial response patients. The study also revealed that overexpression of ASPH mimics the effects of 2-OG, and knockdown of ASPH improves chemotherapy. Targeting ASPH enhances the effects of chemotherapy by modulating ATM and ATR, two key regulators of DDRs.