期刊
BIOSENSORS & BIOELECTRONICS
卷 72, 期 -, 页码 95-99出版社
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2015.04.021
关键词
Fluorescent assay; Functional nucleic acid; Hg2+ detection; Pb2+ detection; T-Hg2+-T; G-quadruplex
类别
资金
- National High Technology Research and Development Program (863 Program) of China [2012AA101405]
- Special Fund for Agro-scientific Research in the Public Interest of China [201503149]
- National Natural Science Foundation of China [31071860, 20977062]
- Municipal Natural Science Foundation of Shanghai, China [13ZR1421700]
- Shanghai Jiaotong University [13 x 100010018]
This paper reports a simple fluorescent assay for the determination of Hg2+ and Pb2+ by using a DNA intercalator GeneFinder (TM) (GF) and an integrated functional nucleic acid (FNA). In the absence of Hg2+ and Pb2+, GF intercalated with the FNA and released moderate strong fluorescence. While in the presence of Hg2+ or Pb2+, the FNA would be induced to form T-Hg2+-T or G-quadruplex structure, interacted with which the GF would exhibit extremely strong or very weak fluorescence. By monitoring the fluorescence changes upon addition of these two ions, the Hg2+ and Pb2+ could be selectively detected as low as 3.23 ppb and 2.62 ppb. As the main advantage of this assay is simplicity and the feasibility was demonstrated by detecting Hg2+ and Pb2+ in spiked water samples, this assay holds great potential for the development of a cost effective and useful tool for environmental monitoring. (C) 2015 Elsevier B.V. All rights reserved.
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