Article
Virology
Junzhuo Si, Xia Tang, Lei Xu, Huichao Fu, Huayi Li, Yonglin He, Jiajia Bao, Jialing Tang, Anlong Li, Nan Lu, Chun Yang
Summary: This study identified significantly altered circRNAs, lncRNAs, miRNAs, and mRNAs pathways in RD cells infected with EV-D68, building a ceRNA regulatory network centered on miRNA. The majority of differentially expressed mRNAs were found to be related to DNA binding, transcription regulation, and various signaling pathways. The hub mRNAs including EGR1, Fos, and Jun were screened out through a protein interaction network. The Fos and ARRDC3 genes were demonstrated to inhibit EV-D68 viral replication in RD cells, suggesting potential drug targets for further research.
Article
Genetics & Heredity
Minqiu Lu, Yin Wu, Wen Gao, Ying Tian, Guorong Wang, Aijun Liu, Wenming Chen
Summary: This study explored the potential effects of novel non-coding ribose nucleic acids (ncRNAs) in patients with multiple myeloma (MM), revealing two new lncRNAs related to MM oncogenes. Additionally, a competitive endogenous RNA network associated with lncRNAs in MM was elucidated through deep RNA-seq analysis.
FRONTIERS IN GENETICS
(2021)
Article
Endocrinology & Metabolism
Xu Zhou, Jingliang He, Jinbo Chen, Yu Cui, Zhenyu Ou, Xiongbing Zu, Nenghui Liu
Summary: This study showed that MEG3 acts as a molecular sponge to absorb miR-93-5p, leading to increased PTEN expression in Leydig cells under orchitis, providing a theoretical basis for establishing potential new treatment strategies.
REPRODUCTIVE BIOLOGY AND ENDOCRINOLOGY
(2021)
Article
Oncology
Chuanhui Chen, Mengzhi Wan, Xiong Peng, Qing Zhang, Yu Liu
Summary: This study examines the function and mechanism of the ceRNA network centered around GPR37 in LUAD. The findings show that high expression of GPR37 in LUAD tissue samples is associated with poor prognosis, and it may regulate the expression of downstream target genes by competitively binding to lncRNA DLEU1 and miR-4458.
TRANSLATIONAL ONCOLOGY
(2024)
Article
Immunology
Danfeng Lu, Zhuoyue Li, Pei Zhu, Zhenxing Yang, Heng Yang, Zhanhong Li, Huachun Li, Zhuoran Li
Summary: This study provides insights into the comprehensive characterization of BTV-host interactome and BTV infection and pathogenic mechanisms through whole-transcriptome sequencing and bioinformatics analysis.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Cell Biology
Pauline Sararols, Isabelle Stevant, Yasmine Neirijnck, Diane Rebourcet, Annalucia Darbey, Michael K. Curley, Francoise Kuhne, Emmanouil Dermitzakis, Lee B. Smith, Serge Nef
Summary: FLCs and ALCs exhibit significant differences in transcriptional and functional features, including gene expression variations and non-transcriptional events such as intron retention. The FLC-specific expression of Crhr1 and Mc2r receptors suggests a dual regulation of steroidogenesis.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Dermatology
Wanni Zhao, Xue Meng, Jianfeng Liang
Summary: This study investigates the role of circRNA and mRNA in the pathogenesis of diabetes mellitus (DM) through high-throughput RNA sequencing. The circRNA-miRNA-mRNA regulatory network is explored, revealing the mechanism of gene expression regulation. The differential expression analysis and pathway enrichment analysis provide insights into the involvement of circRNA and mRNA in DM progression.
INTERNATIONAL WOUND JOURNAL
(2022)
Article
Biochemistry & Molecular Biology
Zijun Xiao, Shanshan Wei, Jie Huang, Jiaqin Liu, Jian Liu, Bikui Zhang, Wenqun Li
Summary: This study systematically investigated alterations in the expression levels of ncRNAs in a DIC mouse model through deep RNA sequencing, identifying aberrantly expressed lncRNAs, circRNAs, miRNAs, and mRNAs. Comprehensive ceRNA networks were constructed and functional enrichment analyses were performed for differentially expressed genes.
DNA AND CELL BIOLOGY
(2022)
Article
Biology
Yifei Gao, Chao Wu, Jiaqi Huang, Zhihong Huang, Zhengsen Jin, Siyu Guo, Xiaoyu Tao, Shan Lu, Jingyuan Zhang, Fanqin Zhang, Yiyan Zhai, Rui Shi, Peizhi Ye, Jiarui Wu
Summary: This study utilized ceRNA chips and bioinformatics methods to investigate the key genes and mechanisms involved in matrine intervention in gastric cancer cells. The results showed that ADAM12 and PDGFRB are the down-regulated key genes after matrine intervention. Two ceRNA regulatory axes related to gastric cancer prognosis were identified, providing important guidance for early diagnosis and treatment of gastric cancer.
COMPUTERS IN BIOLOGY AND MEDICINE
(2023)
Article
Biochemical Research Methods
Litao Li, Jinming Cheng, Yingxiao Ji, Jihong Liu, Rui Zhai, Hebo Wang
Summary: This study investigates the neurogenesis targets of curcumin action in mouse brain with middle cerebral artery occlusion (MCAO) using high-throughput sequencing and gene ontology analyses. The results reveal that curcumin exerts neuroprotective effects by regulating neurogenesis and provide potential therapeutic targets for curcumin in MCAO.
COMBINATORIAL CHEMISTRY & HIGH THROUGHPUT SCREENING
(2023)
Article
Plant Sciences
Xifang Zong, Shengsheng Wang, Yangyang Han, Qiang Zhao, Pan Xu, Qi Yan, Fan Wu, Jiyu Zhang
Summary: Salt stress is a major abiotic stress for crop productivity. This study identified a large number of lncRNAs in Melilotus albus, with higher tissue-specificity in response to salt stress compared to mRNAs. The lncRNAs were found to potentially regulate the expression of target genes involved in various biological processes through cis, trans and miR-mediated mechanisms.
ENVIRONMENTAL AND EXPERIMENTAL BOTANY
(2021)
Review
Microbiology
Tara E. Ness, Andrew DiNardo, Maha R. Farhat
Summary: High throughput sequencing (HTS) can detect the presence of Mycobacterium tuberculosis DNA in clinical samples and provide information on drug susceptibility. Multiple studies have laid the groundwork for exploring the clinical application of HTS in tuberculosis diagnosis. This article presents the workflow challenges, strengths, and limitations of different sequencing platforms, as well as the tools used for analysis, to provide a framework for further innovations in the field.
Review
Medicine, General & Internal
Xuejun He, Ningzhi Zhang, Wenye Cao, Yiqiao Xing, Ning Yang
Summary: Advancements in high-throughput sequencing (HTS) technology have provided new insights into the etiology, signaling pathways, and biomarkers of ocular diseases, aiding in improving their diagnosis and research.
JOURNAL OF CLINICAL MEDICINE
(2022)
Article
Biochemistry & Molecular Biology
Piotr Pawlicki, Anna Galuszka, Laura Pardyak, Ryszard Tuz, Bartosz J. Plachno, Martyna Malopolska, Klaudia Dubniewicz, Ping Yang, Malgorzata Kotula-Balak, Kazimierz Tarasiuk
Summary: The study explores the functional status of Leydig cells after immunocastration, providing important knowledge for the diagnosis and treatment of testis dysfunction caused by hormone imbalances and Leydig cell abnormalities.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biotechnology & Applied Microbiology
Aziz M. Al'Khafaji, Jonathan T. Smith, Kiran V. Garimella, Mehrtash Babadi, Victoria Popic, Moshe Sade-Feldman, Michael Gatzen, Siranush Sarkizova, Marc A. Schwartz, Emily M. Blaum, Allyson Day, Maura Costello, Tera Bowers, Stacey Gabriel, Eric Banks, Anthony A. Philippakis, Genevieve M. Boland, Paul C. Blainey, Nir Hacohen
Summary: Full-length RNA-sequencing methods using long-read technologies are limited in throughput. We introduce MAS-ISO-seq, a technique that programmably concatenates cDNAs for long-read sequencing, increasing throughput by >15-fold to nearly 40 million cDNA reads per run. Applied to single-cell RNA sequencing of tumor-infiltrating T cells, MAS-ISO-seq showed a 12- to 32-fold increase in the discovery of differentially spliced genes.
NATURE BIOTECHNOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Wei Zhou, Li Su, Xingyu Duan, Xi Chen, Aislinn Hays, Satya Upadhyayula, Juili Shivde, Huizhi Wang, Yong Li, Dingming Huang, Shuang Liang
MOLECULAR IMMUNOLOGY
(2018)
Article
Toxicology
Han Aijie, Zou Lingyue, Gan Xiaoqin, Li Yu, Liu Fangfang, Chang Xuhong, Zhang Xiaotian, Tian Minmin, Li Sheng, Su Li, Sun Yingbiao
TOXICOLOGY LETTERS
(2018)
Article
Environmental Sciences
Xiaoqin Gan, Xiaotian Zhang, Qiannan E, Qiong Zhang, Yixing Ye, Yunyu Cai, Aijie Han, Minmin Tian, Caixia Wang, Zheng Su, Li Su, Changhao Liang
ENVIRONMENTAL TOXICOLOGY
(2019)
Article
Toxicology
Xiaotian Zhang, Xiaoqin Gan, E. Qiannan, Qiong Zhang, Yixing Ye, Yunyu Cai, Aijie Han, Minmin Tian, Yixuan Wang, Caixia Wang, Li Su, Changhao Liang
TOXICOLOGY MECHANISMS AND METHODS
(2019)
Article
Public, Environmental & Occupational Health
Xuxia Wang, Li Su, Hongwen Zhu, Wenbiao Hu, Jing An, Caixia Wang, E. Qiannan, Xin Qi, Guihua Zhuang
AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE
(2020)
Article
Biotechnology & Applied Microbiology
Caixia Wang, Li Su, Qiuling Mu, Xueyan Gu, Xuejun Guo, Xuxia Wang
Summary: The first Chinese domestic 13-valent pneumococcal conjugate vaccine (WoAnxin (R), PCV-13) is cost-effective for children aged 2 to 5 years, and should be prioritized in China's national immunization program according to the study's findings.
HUMAN VACCINES & IMMUNOTHERAPEUTICS
(2021)
Article
Environmental Sciences
Caixia Wang, Zhangyu Gu, Xueyan Gu, Xinyue Tan, Shuang Wang, Rui Zhang, Ruifen Li, Mingkun Sun, Chunyan Gui, Sheng Li, Yixing Ye, Jianhua Ma, Li Su, Changhao Liang
Summary: The study demonstrated that Nano-Se effectively prevented nickel-induced hepatotoxicity by enhancing hepatic antioxidant capacity, reducing cellular oxidative stress, and improving nuclear structure morphology, indicating its protective effects against nickel-induced liver damage in both in vivo and in vitro models.
ENVIRONMENTAL TOXICOLOGY
(2022)
Article
Clinical Neurology
Peng Li, Zhen Liang, Zhaojing Yuan, Guohua Li, Yanni Wang, Wei Huang, Lingyun Zeng, Jiezhi Yang, Xin Zhou, Junchang Li, Li Su, Yongjie Zhou
Summary: This study found that sustained stress during COVID-19 is associated with depression in front-line medical staff. Insomnia serves as a mediator in the relationship between perceived stress and depression, while resilience moderates the effect of perceived stress on depression.
JOURNAL OF AFFECTIVE DISORDERS
(2022)
Article
Public, Environmental & Occupational Health
Xuxia Wang, Li Su, Shougang Sun, Wenbiao Hu, Qiuling Mu, Xuefeng Liang, Na Jin, Tian Dai, Hui Li, Guihua Zhuang
Summary: The study evaluated the long-term neurological sequelae and disease burden of Japanese encephalitis in Gansu, China. Results showed that JE patients had severe neurological deficits, subnormal intelligence and memory function, with a higher prevalence in adults. The disease burden was found to be significantly higher for adults compared to younger subjects.
ANNALS OF GLOBAL HEALTH
(2021)
Article
Toxicology
Kathryn Page, Walter Westerink, Kristie Sullivan, Thomas McDonald, Clive Roper
Summary: This study developed a new method to assess the skin irritation of antimicrobial cleaning products. The method utilized a more human-like model and demonstrated its effectiveness through comparison with in vivo rabbit skin irritation data.
TOXICOLOGY IN VITRO
(2024)
Article
Toxicology
Arupam Patra, Thirukumaran Kandasamy, Siddhartha Sankar Ghosh, Gurvinder Kaur Saini
Summary: This study successfully produced recombinant anisoplin and demonstrated its significant anti-cancer effect and ability to induce apoptosis in breast cancer cells. The activation of related signaling pathways may be the key to cell death.
TOXICOLOGY IN VITRO
(2024)
Article
Toxicology
Thomas Haller, Alexander Jesacher, Alberto Hidalgo, Christina Schmidt
Summary: This study used live cell imaging to observe the accumulation of amiodarone in primary rat alveolar type II cells, and found that it specifically accumulates in lamellar bodies. The uptake is rapid, while storage is persistent. The main mechanisms for intracellular bioaccumulation of amiodarone are proposed to be passive diffusion, ion-trapping, and lipophilic interactions.
TOXICOLOGY IN VITRO
(2024)
Article
Toxicology
Filiz Bakar-Ates, Erva Ozkan
Summary: This study investigated the ferroptotic effect of CuB in breast cancer cells and evaluated its combination with erastin, a ferroptosis inducer. The results showed that the combination treatment significantly activated the ferroptotic pathways and altered the expression of iron-related proteins in breast cancer cells.
TOXICOLOGY IN VITRO
(2024)
Article
Toxicology
Rachel Z. Bennie, Ian C. Shaw
Summary: Estrogen mimics are synthetic and naturally occurring compounds that can interact with estrogen receptors in animals. In vitro transactivation reporter gene assay and in silico molecular modelling can be used to predict the mimicry of these compounds, reducing reliance on animal studies.
TOXICOLOGY IN VITRO
(2024)
Article
Toxicology
Sevda Isik, Semra Cicek
Summary: This study found that high doses of riboflavin can cause cytotoxicity in rainbow trout gonad cells and affect the transcriptional expressions of antioxidant enzymes and growth and reproductive genes, potentially leading to DNA damage and cell death.
TOXICOLOGY IN VITRO
(2024)
Article
Toxicology
Ying Wang, Jie Wu, Mengqi Wan, Dou Yang, Fasheng Liu, Kehao Li, Manxin Hu, Yuanyuan Tang, Huiqiang Lu, Shouhua Zhang, Yuanzhen Xiong
Summary: m-Cresol is commonly used as an intermediate for pesticides and other industrial applications. This study investigated the hepatotoxicity of m-cresol using zebrafish larvae and explored its molecular mechanisms. The results suggest that m-cresol may induce liver damage in zebrafish larvae through oxidative stress and cell apoptosis pathways.
TOXICOLOGY IN VITRO
(2024)
Article
Toxicology
Vincent J. Manna, Shannon Dwyer, Vanessa Pizutelli, Salvatore J. Caradonna
Summary: The widespread use of electronic cigarettes and the emergence of a new illness have raised concerns about the effects of e-cigarette vapor on respiratory tissues. Researchers have developed a simple device to mimic the response of human airway tissue after long-term exposure to e-cigarette vapor, and have identified differences in the effects of different vapor compositions on airway tissue.
TOXICOLOGY IN VITRO
(2024)
