Article
Pharmacology & Pharmacy
Muraly Puttabyatappa, Nadia Saadat, Venkateswaran Ramamoorthi Elangovan, John Dou, Kelly Bakulski, Vasantha Padmanabhan
Summary: Gestational exposure to Bisphenol A (BPA) leads to insulin resistance and oxidative stress in the liver and skeletal muscle of adult female sheep offspring. Transcriptomic analysis revealed dysregulated gene expression and altered non-coding RNA profiles in the liver and muscle of BPA-exposed sheep. These findings provide insight into the molecular mechanisms underlying BPA-induced metabolic dysfunctions in prenatal development.
TOXICOLOGY AND APPLIED PHARMACOLOGY
(2022)
Article
Nutrition & Dietetics
Hun Hwan Kim, Sang Eun Ha, Min Yeong Park, Se Hyo Jeong, Pritam Bhagwan Bhosale, Abuyaseer Abusaliya, Chung Kil Won, Jeong Doo Heo, Meejung Ahn, Je Kyung Seong, Hyun Wook Kim, Gon Sup Kim
Summary: Using RNA sequencing, this study identified KYNA as a potential therapeutic target for gastric cancer. KYNA treatment inhibited cancer cell growth and proliferation, while inducing cell death. This research can provide valuable insights for the development of drugs targeting gastric cancer.
Article
Biotechnology & Applied Microbiology
Yin Ji Piao, Hoe Suk Kim, Wonshik Han, Woo Kyung Moon
Summary: SerpinB2 deficiency alters the expression of multiple genes in mammary tumors, which might cause a delay in PyMT-induced mammary tumor progression.
Article
Immunology
Honghao Yin, Rui Guo, Huanyu Zhang, Songyi Liu, Yuehua Gong, Yuan Yuan
Summary: The study identified key roles of tissue microenvironment cells, particularly macrophages, fibroblasts, and endothelial cells, in the development of gastric cancer, suggesting potential targets for early detection, diagnosis, and treatment strategies.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Oncology
Noortje Verschoor, Marcel Smid, Agnes Jager, Stefan Sleijfer, Saskia M. Wilting, John W. M. Martens
Summary: In this study, the researchers investigated the characteristics and therapy resistance of HER2-positive breast cancer through integrating genomics and transcriptomics data. They found that the genomic profiles of primary and metastatic HER2-positive breast cancers were similar, and certain genomic features were predictive of progression-free survival on post-biopsy anti-HER2 treatment. Additionally, a HER2-driven expression profile grouped different types of HER2-positive tumors and indicated the possibility of transformation to ER independence. Integrated genomic and transcriptomic analyses may play a key role in establishing therapeutic options.
BREAST CANCER RESEARCH
(2023)
Article
Cell Biology
Bijesh George, Bin Gui, Rajeswari Raguraman, Aswathy Mary Paul, Harikrishna Nakshatri, Madhavan Radhakrishna Pillai, Rakesh Kumar
Summary: This study reveals the impact of AKT1-specific transcriptomic changes on breast cancer cells and identifies the cellular functions of AKT1. The study unexpectedly discovers the inherent role of AKT1 in inhibiting the expression of certain genes in breast cancer cells. The findings provide a resource for further understanding of AKT1-dependent modulation of gene expression in breast cancer cells.
Article
Cell Biology
Jun Lin, Deyi Feng, Jie Liu, Ye Yang, Xujin Wei, Wenqia Lin, Qun Lin
Summary: In this study, a single-cell level tool was constructed to predict the prognosis of breast cancer patients using gene set enrichment analysis. The results indicate that the stemness-risk gene score (SGS) is a reliable tool for predicting prognosis and response to immunotherapy in breast cancer patients.
Article
Oncology
Seong Min Kim, Shailima Rampogu, Preethi Vetrivel, Apoorva M. Kulkarni, Sang Eun Ha, Hun Hwan Kim, Keun Woo Lee, Gon Sup Kim
Summary: In this study, high-throughput RNA sequencing was used to identify differentially expressed genes in HepG2 cells after treatment with SIN, revealing 10 upregulated hub genes closely related to cancer progression that are involved in regulating apoptotic processes, immune response, and tumor necrosis factor production. This study provides insights into potential therapeutic strategies targeting these genes for the treatment of liver cancer.
Article
Biochemistry & Molecular Biology
Olivia M. Marx, Marc M. Mankarious, Melanie A. Eshelman, Wei Ding, Walter A. Koltun, Gregory S. Yochum
Summary: Despite a global decrease in colorectal cancer (CRC) incidence, the prevalence of early-onset colorectal cancer (EOCRC) has steadily increased. The understanding of genetic and molecular basis of EOCRC is limited. This study identified increased expression of c-MYC proto-oncogene (MYC) and its downstream targets in tumor samples. Elevated expression of the PVT1 long-non-coding RNA (lncRNA) was also detected. The findings suggest that deregulated MYC contributes to CRCs in younger patients.
Article
Biochemistry & Molecular Biology
Florian Le Billan, Julie Perrot, Elena Carceller, Simon Travers, Say Viengchareun, Peter Kolkhof, Marc Lombes, Jerome Fagart
Summary: The study analyzed the effects of two MRAs on the aldosterone-induced transcriptome in a human renal cell line, revealing the regulation of hundreds of genes by aldosterone and the quantitative efficiency of finerenone on certain aldosterone-induced genes. The data provides insights into the mechanisms of MR signaling pathway and potential therapeutic targets for renal dysfunction.
Article
Oncology
Allison R. Baker, Christos Miliotis, Julia Ramirez-Moya, Talia Marc, Ioannis S. Vlachos, Pilar Santisteban, Frank J. Slack
Summary: ADAR1 plays a critical role in triple-negative breast cancer, and its loss leads to reduced cell growth, invasion, and mammosphere formation. ADAR1 regulates both coding and noncoding targets by altering gene expression level, A-to-I editing, and splicing. The study also reveals that several tumor suppressor miRNAs are upregulated upon ADAR1 loss, suppressing cell-cycle progression and invasion.
MOLECULAR CANCER RESEARCH
(2022)
Article
Biochemistry & Molecular Biology
Kun Xu, Runtian Wang, Qin Chen, Yiqiu Liu, Xintong Li, Ling Mao, Cenzhu Wang, Fangyan Gao, Longfei Hu, Hui Xie, Cong Wang, Guohua Zhou, Xiaoxiang Guan
Summary: This study integrates single-cell RNA sequencing and spatial transcriptomics to investigate the impact of breast cancer cells on the lymph node microenvironment. It reveals the existence of tumor-associated macrophages and their distribution among metastatic breast cancer cells. The study also demonstrates that TAMs are enriched in protumoral pathways in lymph nodes infiltrated by breast cancer cells. Furthermore, it uncovers the potential differentiation trajectory of macrophages from chemokine production to lymphocyte activation.
ACTA BIOCHIMICA ET BIOPHYSICA SINICA
(2022)
Article
Oncology
Anastasia A. Ionkina, Gabriela Balderrama-Gutierrez, Krystian J. Ibanez, Steve Huy D. Phan, Angelique N. Cortez, Ali Mortazavi, Jennifer A. Prescher
Summary: The study analyzed transcriptomes of metastatic breast cancer cells using the MMTV-PyMT mouse model to understand tumor heterogeneity and organ tropism. The results provided insights into metastatic progression and potential targets for breast cancer treatment, as well as the impact of tumor heterogeneity on metastasis.
BREAST CANCER RESEARCH
(2021)
Article
Biochemistry & Molecular Biology
Chih-Chieh Chen, Yung-Che Chou, Chia-Yi Hsu, Eing-Mei Tsai, Tze-Kiong Er
Summary: In this study, RNA sequencing analysis was used to identify a potential pathogenic gene of endometriosis. The differentially expressed genes were found to be enriched in several signaling pathways, providing new insights into the underlying mechanisms of endometriosis.
Article
Oncology
Zheng Wang, Chengyan Chai, Rui Wang, Yimei Feng, Lei Huang, Yiming Zhang, Xia Xiao, Shijie Yang, Yunfang Zhang, Xi Zhang
Summary: This study utilized advanced single-cell RNA sequencing to construct a comprehensive atlas of over 130,000 single-MSC transcriptomes across multiple tissues and donors, revealing seven tissue-specific and five conserved MSC subpopulations with distinct gene expression signatures. The study demonstrated the significant contribution of extracellular matrix to MSC heterogeneity and identified potential improvements in MSC-based therapeutic efficacy.
CLINICAL AND TRANSLATIONAL MEDICINE
(2021)
