Article
Environmental Sciences
Ron L. A. P. Hoogenboom, Guillaume ten Dam, Stefan P. J. van Leeuwen, Harry van Egmond, Jennyfer Nicolina, Arnold J. S. Dwarkasing
Summary: Samples of adipose tissue, meat, and livers from pigs, cows, sheep, and goats in Curacao were found to have higher levels of PCDD/Fs and PCBs than EU maximum levels, with burning of waste being the major source. Meat and adipose tissue showed similar lipid-based levels, while liver levels were significantly higher, indicating differences in contamination levels between pigs and ruminants. Measures were taken to reduce contamination in areas where animals forage, as high levels observed are likely to result in increased consumer exposure.
Article
Agriculture, Dairy & Animal Science
Giovanni Bittante, Nicolo Amalfitano, Matteo Bergamaschi, Nageshvar Patel, Mohamed-Laid Haddi, Hamida Benabid, Michele Pazzola, Giuseppe Massimo Vacca, Franco Tagliapietra, Stefano Schiavon
Summary: Bovine milk accounts for 83% of the milk and dairy products consumed by humans worldwide. The efficiency of cheese-making differs among different species, with buffalo and ewe milk yielding the best cheese.
JOURNAL OF DAIRY SCIENCE
(2022)
Article
Multidisciplinary Sciences
Maki Hirata, Manita Wittayarat, Zhao Namula, Quynh Anh Le, Qingyi Lin, Koki Takebayashi, Chommanart Thongkittidilok, Taro Mito, Sayuri Tomonari, Fuminori Tanihara, Takeshige Otoi
Summary: The study developed a novel lipofection-mediated RNP transfection technique for generating gene-edited pigs without the need for specialized equipment, producing no detectable off-target events. Despite the need for improvements in editing efficiency, this practical method for rapid and large-scale gene editing in pigs has important agricultural and biomedical applications.
SCIENTIFIC REPORTS
(2021)
Article
Agriculture, Dairy & Animal Science
Lenka Valkova, Vladimir Vecerek, Eva Voslarova, Michal Kaluza, Daniela Takacova
Summary: The study evaluated traumatic injuries in cattle, sheep, goats, and pigs in the Czech Republic. Traumatic lesions were found at a low frequency, with cows being the most affected category and small ruminants and bulls having lower rates of traumatic findings. This suggests that factors such as animal housing and rearing environment may impact the occurrence of traumatic injuries.
Article
Biochemistry & Molecular Biology
Asim Shahzad, Fahim Ullah, Hamid Irshad, Shehzad Ahmed, Qismat Shakeela, Abrar Hussain Mian
Summary: This study investigated the prevalence of E. coli O157 in slaughtered adult sheep, goats, cows, and buffaloes, finding that healthy adult sheep and buffalo could potentially carry STEC O157. Of the positive samples, one buffalo isolate carried multiple STEC virulent genes, highlighting the importance of proper control systems to prevent STEC infections from these animals.
MOLECULAR BIOLOGY REPORTS
(2021)
Article
Veterinary Sciences
Aiden P. Foster
Summary: The aim of this article is to provide an overview of common skin conditions in livestock species, including ectoparasites, immune-mediated disease, and allergic dermatitis. It emphasizes the importance of thorough clinical examination, differential diagnosis, and appropriate diagnostic testing before treatment. The article also discusses the use of topical antimicrobial therapies and the potential risks of anthelmintic resistance.
JAVMA-JOURNAL OF THE AMERICAN VETERINARY MEDICAL ASSOCIATION
(2023)
Article
Biochemistry & Molecular Biology
Liu Yang, Lei Huang, Yulian Mu, Kui Li
Summary: Long-term high-energy intake has detrimental effects on pig health and elevates the risk of metabolic disease. In this study, we profiled the A-to-I editing in three tissues and six gut segments of model pigs and characterized their functional aspect in relation to metabolic disorders. We detected a large number of A-to-I editing sites across the pig genome, with highest editing levels in the abdominal aorta. The significant difference in editing levels between high-energy induced and control pigs was found in multiple tissues and gut segments. Our work provides valuable knowledge of A-to-I editing sites functionally involved in the development of metabolic disorders.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Medicine, Research & Experimental
Yan Li, Di Lian, Jiahao Wang, Yue Zhao, Yao Li, Guoshi Liu, Sen Wu, Shoulong Deng, Xuguang Du, Zhengxing Lian
Summary: CRISPR-Cas9-mediated genome editing is useful in sheep for both agricultural and biomedical applications. However, the efficiency of knockin via homology-directed repair (HDR) is lower in sheep fetal fibroblasts (SFFs), hindering precise genome editing. This study optimized key parameters affecting HDR in SFFs and identified small molecules that improved HDR efficiency by activating the p53 pathway. Additionally, genetic knockout of p53 suppressed HDR in SFFs. Overall, this study provides an optimized strategy for dsDNA repair template usage and a simple, efficient method for CRISPR-Cas9-mediated precise genome editing in sheep primary cells.
MOLECULAR THERAPY-NUCLEIC ACIDS
(2023)
Article
Virology
Boitumelo Magret Modise, Tirumala Bharani Kumar Settypalli, Tebogo Kgotlele, Dingrong Xue, Kebonyemodisa Ntesang, Kago Kumile, Ivancho Naletoski, John Frederick Nyange, Carter Thanda, Kenny Nametso Macheng, Chandapiwa Marobela-Raborokgwe, Gerrit Johannes Viljoen, Giovanni Cattoli, Charles Euloge Lamien
Summary: This study provides the first genetic characterization of poxvirus diseases in cattle, goats, and sheep in Botswana, highlighting the importance of molecular methods in diagnosing poxvirus diseases of ruminants. The study identified specific poxvirus strains in different hosts and showed the genetic similarities and differences between the isolates in Botswana and those in other regions.
Article
Biochemistry & Molecular Biology
Tatyana Belinskaya, Ashima Saxena
Summary: This study aimed to determine the suitability of bovine, ovine, caprine, and porcine milk for the production of recombinant human butyrylcholinesterase (rHu BChE) by examining the presence of endogenous cholinesterases (ChE) in the milk. The results showed that bovine, ovine, and caprine milk contained both acetylcholinesterase (AChE) and BChE activities, while caprine and porcine milk primarily contained BChE activity. This suggests that cows, sheep, and goats are the ideal choices for transgenic expression of rHu BChE in milk.
CHEMICO-BIOLOGICAL INTERACTIONS
(2023)
Article
Agriculture, Dairy & Animal Science
Konstantinos V. Arsenopoulos, George C. Fthenakis, Eleni I. Katsarou, Elias Papadopoulos
Summary: This paper reviews the challenges of haemonchosis, a common parasitic infection in sheep and goats caused by Haemonchus parasites. Challenges include varying global prevalence, parasite resistance to antiparasitic drugs, difficulties in accurate diagnosis, and effective infection control.
Article
Biology
Honghui Li, Wenmin Cheng, Bowei Chen, Shaoxia Pu, Ninglin Fan, Xiaolin Zhang, Deling Jiao, Dejia Shi, Jianxiong Guo, Zhuo Li, Yubo Qing, Baoyu Jia, Hong-Ye Zhao, Hong-Jiang Wei
Summary: The BE3 system utilizes CRISPR molecules to edit the pig P53 gene, obtaining a pig model with a P53 point mutation for further research on the mechanism of tumorigenesis and improvement in tumor diagnosis and treatment.
Article
Veterinary Sciences
Alina Marandykina-Prakiene, Dalius Butkauskas, Naglis Gudiskis, Evelina Juozaityte-Ngugu, Dovile Laisvune Bagdonaite, Muza Kirjusina, Rafael Calero-Bernal, Petras Prakas
Summary: Contradictory data on intermediate host specificity of Sarcocystis spp. in farm animals exists. This study molecularly tested samples from sheep and goats in Lithuania to identify Sarcocystis species described in other intermediate hosts but suspected to be non-canonical parasites to these small ruminants. The findings revealed the presence of previously thought to be host-specific Sarcocystis species in different hosts, highlighting the need for further research.
VETERINARY SCIENCES
(2023)
Article
Agriculture, Dairy & Animal Science
John W. Walker, Scott L. Kronberg
Summary: This study investigated the effect of rearing environment on the consumption of leafy spurge by sheep and goats. The results showed that goats consumed more leafy spurge compared to sheep. It was also found that rumen microbes obtained from goats may help sheep to detoxify plant toxins. The study suggests that in addition to genetic factors, an animal's microbiome genome may play an important role in diet selection.
Review
Agriculture, Dairy & Animal Science
J. Simoes, J. A. Abecia, A. Cannas, J. A. Delgadillo, D. Lacasta, K. Voigt, P. Chemineau
Summary: This review discusses the importance of nutritional, reproductive, and health management in the intensification of sheep and goat production, emphasizing the significant impact of nutrition on flock health, product quality, and environmental sustainability. Advanced reproductive technologies and health management strategies play a crucial role in improving production efficiency and genetic improvement in highly productive systems, while genomic research and technological innovation are key to sustaining sheep and goat production in the future.
Article
Biochemistry & Molecular Biology
Ana Paula Arevalo, Romina Castelli, Manuel Ibarra, Martina Crispo, Victoria Calzada
Summary: This study evaluated the use of Sgc8-c aptamer as an imaging probe in a colon tumor xenograft mouse model, showing its excellent tumor imaging effect and high specificity. It has potential clinical applications for colon cancer.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Agriculture, Dairy & Animal Science
Jose Renato S. Passos, Denise D. Guerreiro, Kamila S. Otavio, Pedro C. dos Santos-Neto, Marcela Souza-Neves, Federico Cuadro, Richard Nunez-Olivera, Martina Crispo, Maria Julia B. Bezerra, Renato F. Silva, Laritza F. Lima, Jose Ricardo Figueiredo, Ivan C. Bustamante-Filho, Alejo Menchaca, Arlindo A. Moura
Summary: The present study characterized the major proteome of preimplantation ovine embryos grown in vitro. The analysis identified proteins that are involved in translation, protein transport, and stabilization. Functional cluster analysis revealed differences between the in vitro and in vivo proteomes of sheep embryos, indicating that in vitro derived blastocysts have distinct protein profiles compared to those produced in vivo.
REPRODUCTION IN DOMESTIC ANIMALS
(2022)
Article
Reproductive Biology
R. Nunez-Olivera, G. A. Bo, A. Menchaca
Summary: This study evaluated the relationship between proestrus length and follicular size, estrous behavior, and pregnancy rate. The results showed that extending proestrus length could increase the diameter of the preovulatory follicle, proportion of heifers expressing estrus, and pregnancy rate in beef heifers.
Article
Genetics & Heredity
Matias Fabregat, Sofia Nino-Rivero, Sabrina Pose, Magdalena Cardenas-Rodriguez, Mariana Bresque, Karina Hernandez, Victoria Prieto-Echaguee, Geraldine Schlapp, Martina Crispo, Patricia Lagos, Natalia Lago, Carlos Escande, Florencia Irigoin, Jose L. Badano
Summary: This study reports the role of CCDC28B in regulating cilia length and behavioral phenotypes. By generating Ccdc28b mutant mice, the researchers found that these mice displayed a mild phenotype but exhibited clear social interaction defects and stereotypical behaviors. This provides a new opportunity to further investigate the genetic interactions in BBS and the association between ciliopathy and behavioral defects.
Article
Biochemistry & Molecular Biology
Jose Renato S. Passos, Denise D. Guerreiro, Kamila S. Otavio, Pedro C. dos Santos-Neto, Marcela Souza-Neves, Federico Cuadro, Richard Nunez-Olivera, Martina Crispo, Fabio R. Vasconcelos, Maria Julia B. Bezerra, Renato F. Silva, Laritza F. Lima, Jose Ricardo Figueiredo, Ivan C. Bustamante-Filho, Alejo Menchaca, Arlindo A. Moura
Summary: The present study evaluated the effects of in vitro maturation (IVM) on the proteome of cumulus-oocyte complexes (COCs) from ewes. The results showed that IVM caused upregulation and downregulation of proteins in COCs. Upregulated proteins in mature COCs after IVM were related to immune response, complement cascade, cell cycle, and extracellular matrix organization, while downregulated proteins in immature COCs after IVM were linked to metabolic processes, immune response, and complement cascade.
MOLECULAR REPRODUCTION AND DEVELOPMENT
(2022)
Article
Virology
Ayelen Toro, Sofia Lage-Vickers, Juan Bizzotto, Felipe Vilicich, Agustina Sabater, Gaston Pascual, Sabrina Ledesma-Bazan, Pablo Sanchis, Maria Sol Ruiz, Ana Paula Arevalo, Jorge L. Porfido, Mercedes Abbate, Rocio Seniuk, Estefania Labanca, Nicolas Anselmino, Nora M. Navone, Daniel F. Alonso, Elba Vazquez, Martina Crispo, Javier Cotignola, Geraldine Gueron
Summary: This study demonstrates the important role of IFN-gamma and ISGs in the response to SARS-CoV-2 infection, suggesting their potential as adjuvant immunotherapy for COVID-19 patients. The analysis showed increased expression of IFN-gamma-associated pathways and ISGs in COVID-19-positive patients, which correlated with viral load and STAT1/JAK2 expression.
Article
Multidisciplinary Sciences
Hellen Daghero, Flora Doffe, Belen Varela, Victoria Yozzi, Jose Manuel Verdes, Martina Crispo, Mariela Bollati-Fogolin, Romina Pagotto
Summary: The NF-kappa B signaling pathway plays a crucial role in the intestine, protecting epithelial cells from harm while also mediating inflammatory diseases. Established NF-kappa B reporter organoids provide a valuable tool for evaluating anti-inflammatory compounds and exploring their effects in the small intestine.
SCIENTIFIC REPORTS
(2022)
Article
Immunology
Anabella A. Barrios, Camila Mouhape, Leonard Schreiber, Linyun Zhang, Juliane Nell, Mariana Suarez-Martins, Geraldine Schlapp, Maria Noel Meikle, Ana Paula Mulet, Tsui-Ling Hsu, Shie-Liang Hsieh, Gustavo Mourglia-Ettlin, Carlos Gonzalez, Martina Crispo, Thomas F. E. Barth, Cecilia Casaravilla, Stephen J. Jenkins, Alvaro Diaz
Summary: Cystic echinococcosis is a disease caused by larval stages of cestode parasites of the Echinococcus granulosus species cluster, with E. granulosus sensu stricto being the main infecting species. The parasites form bladder-like structures called hydatids in the internal organs of livestock ungulates and humans. The hydatids are protected by a large acellular laminated layer (LL) composed mainly of mucins. This study found that LL mucins injected into mice are taken up by liver macrophages called Kupffer cells, primarily through a receptor called Clec4F. These findings suggest that Kupffer cells play a role in clearing LL materials from the host.
INFECTION AND IMMUNITY
(2023)
Article
Microbiology
Paula Faral-Tello, Gonzalo Greif, Selva Romero, Andres Cabrera, Cristina Oviedo, Telma Gonzalez, Gabriela Libisch, Ana Paula Arevalo, Belen Varela, Jose Manuel Verdes, Martina Crispo, Yester Basmadjian, Carlos Robello
Summary: This study discovered Trypanosoma cruzi strains capable of vertical transmission, which have a higher transmission rate compared to strains of moderate or high virulence. The vertical transmission strains modulate placental gene expression, inducing anti-inflammatory responses and immune damage. This finding provides new insights into the mechanism of vertical transmission for this parasite.
MICROBIOLOGY SPECTRUM
(2023)
Article
Biochemistry & Molecular Biology
Rossina Novas, Tatiana Basika, Megan E. Williamson, Pablo Fresia, Alejo Menchaca, Maxwell J. Scott
Summary: The New World screwworm, a major pest of livestock, was successfully eradicated from North and Central America using the sterile insect technique. However, more cost-effective genetic methods may be needed to control this parasite in South America. The recent development of CRISPR/Cas9-based genetic approaches, such as homing gene drive, could provide an efficient means to suppress screwworm populations.
INSECT MOLECULAR BIOLOGY
(2023)
Article
Reproductive Biology
P. C. dos Santos-Neto, F. Cuadro, M. Souza-Neves, M. Crispo, A. Menchaca
Summary: In this study, the developmental competence, pregnancy outcomes, and embryo survival and birth rate of CRISPR/Cas microinjected zygotes in large scale programs in sheep were evaluated. The results showed that the developmental rate of microinjected zygotes was lower than non-injected zygotes, but similar outcomes were achieved when the embryos were transferred into the uterine horn instead of the oviduct. Furthermore, vitrification of CRISPR/Cas microinjected embryos did not significantly affect pregnancy rate, embryo survival rate, and birth rate.
Review
Agriculture, Dairy & Animal Science
Isabel Oliveira Cosentino, Mario Felipe Alvarez Balaro, Alejo Menchaca, Raquel Perez-Clariget, Rodolfo Ungerfeld, Felipe Zandonadi Brandao
Summary: Hormonal methodologies are widely used and evolved to control small ruminants' estrous cycle, aiming to optimize reproductive performance. The induced and/or synchronized estrous cycle can be utilized for fixed-time artificial insemination or based on estrus behavior signs for natural or guided mating. Resynchronization protocols have been developed to increase reproductive outcomes in females that failed to conceive, aiming to resynchronize ovulation as early as non-pregnancy is detected. This review provides a summary of recent advances and main findings regarding resynchronization protocols in small ruminants, as well as future perspectives and new research directions. Although the field of resynchronization treatment in small ruminant reproduction is still developing, enhancements in reproductive outcomes indicate that these protocols can be successfully utilized in sheep and goat production.
ANIMAL REPRODUCTION
(2023)
Meeting Abstract
Biochemical Research Methods
Jorge Porfido, Maria Noel Meikle, Geraldine Schlapp, Alejo Menchaca, Martina Crispo
TRANSGENIC RESEARCH
(2022)
Article
Reproductive Biology
Haolin Li, Xiaomeng Pei, Hao Yu, Wei Wang, Dagan Mao
Summary: This study investigated the effects of APN/AdipoRon on autophagy and apoptosis in goat luteal cells, and found that autophagy and apoptosis play important roles in the structural regression of goat corpus luteum.
Article
Reproductive Biology
Yan-Qiu Wang, He-Xuan Qu, Yan-Wei Dong, Jia-Jia Qi, Hua-Kai Wei, Hao Sun, Hao Jiang, Jia-Bao Zhang, Bo-Xing Sun, Shuang Liang
Summary: This research reveals the crucial role of Ferroptosis Suppressor Protein 1 (FSP1) in regulating the development and quality of porcine early embryos. Inhibition of FSP1 impairs blastocyst formation, induces glutathione-independent ferroptosis, and leads to oxidative stress due to mitochondrial dysfunction.
Article
Reproductive Biology
Xiong Lai, Ruizhuo Liu, Mengyu Li, Yaochun Fan, Hongxia Li, Guotao Han, Ruijie Guo, Hairui Ma, Huimin Su, Wanjin Xing
Summary: This study identifies WDR54 as a protein involved in sperm-oocyte fertilization and shows that it interacts with IZUMO1 and JUNO. The formation of the JWIJ complex on the oocyte surface suggests its importance in sperm-oocyte adhesion and fusion. This discovery provides new insights into the mechanisms of mammalian sperm-oocyte adhesion and fusion.
Article
Reproductive Biology
S. Nandi, B. Sampath Kumar, P. S. P. Gupta, S. Mondal, V. Girish Kumar
Summary: This study examined the effects of plant bioactive compounds on the survival and growth parameters of ovine preantral follicles granulosa cells (GCs) and the expression of key developmental genes. The results showed that KAE, MYR and QUE had dose-dependent responses on the morphological and functional parameters of the cells, with KAE being the most potent in augmenting ovarian functions.
Article
Reproductive Biology
Francisco E. Martin-Cano, Gemma Gaitskell-Phillips, Laura Becerro-Rey, Eva da Silva, Javier Masot, Eloy Redondo, Antonio Silva-Rodriguez, Cristina Ortega-Ferrusola, Maria Cruz Gil, Fernando J. Pena
Summary: The presence of a more efficient glycolysis mechanism depending on pyruvate in stallion spermatozoa can counteract the detrimental effects of higher glucose concentrations. Incubating spermatozoa with 10 mM pyruvate in a modified Tyrode's media with 67 mM glucose showed increased motility and maintained viability. This improvement is attributed to the conversion of lactate to pyruvate and increased NAD(+) enhancing glycolysis efficiency.
Article
Reproductive Biology
Thanida Sananmuang, Denis Puthier, Catherine Nguyen, Kaj Chokeshaiusaha
Summary: Ongoing progress in mRNA-Sequencing technologies has contributed to the refinement of assisted reproductive technologies. This study utilized mRNA-Seq datasets of various mammalian species to analyze differential transcript usage (DTU) and identified ten cross-species DTU genes. Gene Ontology (GO) term enrichment analysis revealed the involvement of these genes in RNA and cell-cycle control mechanisms, suggesting their importance during oocyte maturation. Further exploration of these genes' transcript isoforms may lead to the discovery of novel markers for reproductive technologies.
Article
Reproductive Biology
Chenglei Song, Zhe Zhang, Yilin Wei, Yaqing Dou, Kunlong Qi, Xiuling Li, Feng Yang, Xinjian Li, Kejun Wang, Ruimin Qiao, Xuelei Han
Summary: In this study, the changes in protein levels in boar sperm with different liquid preservation abilities were analyzed. Key proteins related to sperm liquid preservation ability were identified. The findings provide new insights into the molecular mechanisms of boar sperm liquid preservation ability.
Article
Reproductive Biology
Genevieve M. VanWye, Emily G. Smith, Christine Spinka, Michael F. Smith, Matthew C. Lucy, Jordan M. Thomas
Summary: A series of experiments evaluated treatment schedules for controlling the estrous cycle in beef heifers. The results showed that the duration of progestin treatment and the timing of prostaglandin administration had an impact on estrus expression and pregnancy rates.
Article
Reproductive Biology
Hongwei Duan, Shuai Yang, Longfei Xiao, Shanshan Yang, Zhenxing Yan, Fang Wang, Xiaofei Ma, Lihong Zhang, Yong Zhang, Junjie Hu, Xingxu Zhao
Summary: This study first elucidated the mechanism by which melatonin regulates progesterone secretion in the corpus luteum of sheep, namely by promoting progesterone secretion through the regulation of autophagy. This has important implications for further exploring the role of melatonin in regulating sheep ovarian function.
Article
Reproductive Biology
Zhijie Zheng, Yingfang Guo, Yonghui Zheng, Haichong Wu
Summary: This study demonstrates that miR-92b plays a protective role in LTA-induced endometritis by targeting FZD10 and reducing the production of pro-inflammatory cytokines, thus alleviating uterine injury.
Article
Reproductive Biology
Areeg Almubarak, Sanghoon Lee, Il-Jeoung Yu, Yubyeol Jeon
Summary: This study found that Nobiletin (NOB) can promote the cryosurvival and embryo development of porcine sperm after in vitro fertilization (IVF). The addition of NOB improves sperm motility, acrosome integrity, viability, and mitochondrial membrane potential, reduces lipid peroxidation, and increases cleavage and blastocyst formation rates. Furthermore, NOB treatment upregulates the expression of genes related to pluripotency and cell differentiation in the resulting embryos.
Article
Reproductive Biology
Yuan Li, Lishu Li, Wenjie Xiong, Xing Duan, Huaming Xi
Summary: This study investigated the cytotoxicity and toxicological mechanisms of fluorochloridone (FLC) on cultured goat Sertoli cells. The results showed that FLC exposure induced oxidative stress, promoted apoptosis and autophagy, inhibited lysosomal biogenesis, and blocked autophagic flux in goat Sertoli cells. FLC also caused mitochondrial dysfunction and disturbed mitophagy, leading to cell apoptosis. The antioxidant N-acetyl-L-cysteine (NAC) reduced FLC-induced ROS accumulation and reversed the disorder of autophagy levels.
Article
Reproductive Biology
Gabriela Ramos Leal, Thais de Almeida Oliveira, Mariana Pedrosa de Paula Guimaraes, Lucas Francisco Leodido Correia, Erlandia Marcia Vasconcelos, Joanna Maria Goncalves Souza-Fabjan
Summary: This study investigated the time course of lipid accumulation during IVM and the effects of lipid modulators on cat oocytes. The results showed that adding lipid modulators can reduce lipid content and improve viability after cryopreservation.
Article
Reproductive Biology
Isabella Rio Feltrin, Amanda Guimaraes da Silva, Cecilia Constantino Rocha, Priscila Assis Ferraz, Paola Maria da Silva Rosa, Thiago Martins, Juliano Coelho da Silveira, Milena Lopes Oliveira, Mario Binelli, Guilherme Pugliesi, Claudia Maria Bertan Membrive
Summary: The study evaluated the effects of E2 treatment on Nelore heifers on day 15 of the estrous cycle, finding that E2 promoted an increase in PGFM concentrations and accelerated functional and structural luteolysis by upregulating the expression of PGR and OXTR. This suggests that within 3 hours after E2 stimulus, the expression of these receptors is associated with triggering luteolysis in cattle.
Article
Reproductive Biology
Aline Matos Arrais, Angelo Jose Burla Dias, Claudio Luiz Melo de Souza, Alinne Gloria Curcio, Marco Roberto Bourg de Mello
Summary: This study evaluated the effect of phospholipase C (PLC) on the capacitation of cryopreserved ovine semen. The results showed that the inhibitor U73122 can prevent premature capacitation and acrosome reaction induced by the freezing procedure, without affecting the kinetics and integrity of the sperm membranes.
