期刊
THERIOGENOLOGY
卷 140, 期 -, 页码 33-43出版社
ELSEVIER SCIENCE INC
DOI: 10.1016/j.theriogenology.2019.08.016
关键词
Zebrafish; Chromosome manipulation; Triploid; Germ cell transplantation; Surrogate reproduction
资金
- Ministry of Education, Youth and Sports of the Czech Republic [LM2018099, CZ.02.1.01/0.0/0.0/16_025/0007370]
- Grant Agency of the University of South Bohemia in Ceske Budejovice [GAJU 034/2017/Z]
- Czech Science Foundation [17-19714Y]
- NAZV [QK1910248]
We report for the first time, a comparison of two approaches for artificially induced triploidy in zebrafish (Danio rerio) using cold shock and heat shock treatments. Of the two methods, heat shock treatment proved more effective with a triploid production rate of 100% in particular females. Subsequently, triploid zebrafish larvae were used as recipients for intraperitoneal transplantation of ovarian and testicular cells originating from vas:EGFP strain in order to verify their suitability for surrogate reproduction. Production of donor-derived sperm was achieved in 23% of testicular cell recipients and 16% of ovarian cell recipients, indicating the suitability of triploids as surrogate hosts for germ cell transplantation. Success of the transplantation was confirmed by positive GFP signal detected in gonads of dissected fish and stripped sperm. Germline transmission was confirmed by fertilization tests followed by PCR analysis of embryos with GFP specific primers. Reproductive success of germline chimera triploids evaluated as fertilization rate and progeny development was comparable to control groups. (C) 2019 Elsevier Inc. All rights reserved.
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