Article
Plant Sciences
Xiangyang Xu, Genqiao Li, Guihua Bai, Amy Bernardo, Brett F. Carver, Paul St Amand, Ruolin Bian
Summary: Leaf rust, a common wheat disease in the Great Plains of the United States, was studied using recombinant inbred lines from 'Bainong 418' and CI 17884. Two resistant genes, QLr.stars-1RS and Lr47, were identified, with the latter being particularly effective in the region. Development of specific markers facilitates the introgression of these resistance genes into locally adapted wheat cultivars and breeding lines.
Article
Plant Sciences
J. A. Kolmer, M. K. Turner, M. N. Rouse, J. A. Anderson
Summary: This study identified the chromosome locations of leaf rust resistance genes in the hard red spring wheat cultivar AC Taber, with significant QTL effects for resistance found on chromosomes 2BS and 3BS. The genes on these chromosomes were found to be associated with adult plant resistance genes Lr13 and Lr74. Newly developed KASP PCR markers linked to these regions can be used for marker-based selection of these resistance genes.
Article
Plant Sciences
Mohamed A. M. Atia, Eman A. El-Khateeb, Reem M. Abd El-Maksoud, Mohamed A. Abou-Zeid, Arwa Salah, Amal M. E. Abdel-Hamid
Summary: This study evaluated the leaf rust resistance of 50 Egyptian wheat varieties and identified 28 leaf rust resistance genes within the germplasm collections. Most varieties showed high to moderate levels of resistance, with some displaying fast rusting behavior. 21 out of 28 tested Lr genes were successfully identified, including only five genes conferring adult plant resistant or slow rusting behavior in the Egyptian collection.
Article
Biochemistry & Molecular Biology
Mengyu Li, Shuqing Zhao, Junyu Yang, Yan Ren, Jun Su, Jiaojie Zhao, Xiaopeng Ren, Chuyuan Wang, Shisheng Chen, Xiumei Yu, Feng Chen, Xiaodong Wang
Summary: Systemic acquired resistance (SAR) is a broad-spectrum plant defense mechanism controlled by the salicylic acid receptor NPR1. In this study, a transgenic wheat line expressing the transcription factor gene HvWRKY6 showed improved resistance to various fungal diseases. Transcriptome sequencing revealed the activation of broad-spectrum defense responses through the activation of the salicylic acid pathway and suppression of the abscisic acid and jasmonic acid pathways.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2022)
Article
Plant Sciences
Ali A. Naz, Annemarie Bungartz, Albrecht Serfling, Mohammad Kamruzzaman, Michael Schneider, Brande B. H. Wulff, Klaus Pillen, Agim Ballvora, Erich-Christian Oerke, Frank Ordon, Jens Leon
Summary: This study investigated the evolutionary and functional divergence of the leaf rust resistance gene Lr21 in diploid and hexaploid wheats through population genetics and high-resolution comparative genomics. The research identified original Lr21 alleles in Aegilops tauschii and discovered a new Lr21-tbk allele in hexaploid wheat cv. Tobak. In addition to seedling resistance, the Lr21 gene was found to confer adult plant field resistance in wheat, with resistance correlated with developmentally dependent variation in Lr21 expression.
PLANT CELL AND ENVIRONMENT
(2021)
Article
Plant Sciences
Andrea Matros, Adam Schikora, Frank Ordon, Gwendolin Wehner
Summary: In this study, a diverse set of spring barley accessions were treated with two strains of the soil bacterium Ensifer meliloti to investigate the genotypic differences in barley's response to N-acyl homoserine-lactone (AHL) and identify genomic regions associated with enhanced resistance to Puccinia hordei. The results demonstrated a positive effect of AHL priming on barley's resistance to P. hordei, and a genome-wide association study identified 11 quantitative trait loci (QTL) associated with AHL priming efficiency.
FRONTIERS IN PLANT SCIENCE
(2023)
Review
Plant Sciences
Johannes Mapuranga, Jiaying Chang, Jiaojie Zhao, Maili Liang, Ruolin Li, Yanhui Wu, Na Zhang, Lirong Zhang, Wenxiang Yang
Summary: Wheat leaf rust is a common disease that threatens global wheat production, and current mitigation approaches include the use of fungicides and resistance genes. However, the continuous use of resistant varieties leads to the evolution of the pathogen and emergence of new virulent races, causing the demise of resistant cultivars. Diploid wheat accessions have been found to confer resistance through haustorium formation, which is not affected by specific pathogen races. Systemic acquired resistance also provides durable resistance against various pathogens.
Article
Plant Sciences
Peipei Zhang, Caixia Lan, Ravi P. Singh, Julio Huerta-Espino, Zaifeng Li, Evans Lagudah, Sridhar Bhavani
Summary: This study identified several genes and loci conferring resistance to leaf rust and stripe rust through genetic and QTL mapping research, including a novel pleiotropic gene conferring resistance to both leaf rust and stripe rust in an Afghan landrace. The discovery of these genes and loci is significant for breeding efforts to develop durable resistance to multiple rust diseases.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Plant Sciences
J. A. Kolmer, A. Herman, J. P. Fellers
Summary: This study genotyped collections of P. triticina from 2011 to 2018 in the United States, identifying eight common genotype groups and two new genotype groups. The study also detected single nucleotide polymorphisms associated with resistance genes.
Article
Plant Sciences
Xiangxi Zhuansun, Junna Sun, Nannan Liu, Shengnan Zhang, Huifang Wang, Zhaorong Hu, Jun Ma, Qixin Sun, Chaojie Xie
Summary: Epidemics of leaf rust pose a threat to wheat production. The discovery of the leaf rust resistance gene LrOft in the durum wheat cultivar Ofanto provides a potential solution. Fine mapping identified a suppressor locus for LrOft on chromosome arm 2AS, narrowing down the potential candidate genes.
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Biotechnology & Applied Microbiology
Chunhong Chen, Matthias Jost, Bethany Clark, Matthew Martin, Oadi Matny, Brian J. Steffenson, Jerome D. Franckowiak, Martin Mascher, Davinder Singh, Dragan Perovic, Terese Richardson, Sambasivam Periyannan, Evans S. Lagudah, Robert F. Park, Peter M. Dracatos
Summary: Leaf rust, caused by Puccinia hordei, is a severe fungal disease globally impacting barley production. Cloning of the Rph15 resistance gene from a wild barley progenitor and development of a KASP marker provide a new avenue for sustained leaf rust control. The resistance mediated by Rph15 and previously thought to be allelic with Rph16 is actually the same, allowing for efficient gene deployment in combating leaf rust.
PLANT BIOTECHNOLOGY JOURNAL
(2021)
Article
Plant Sciences
P. M. Dracatos, R. F. Park, D. Singh
Summary: The development and validation of codominant insertion-deletion (indel) based PCR markers provide an efficient approach for selecting genotypes with multiple effective resistance genes for barley leaf rust, enabling effective gene deployment and stewardship.
Article
Plant Sciences
Mathieu Deblieck, Frank Ordon, Albrecht Serfling
Summary: Wheat leaf rust is a significant fungal disease of wheat, and its control measures often break down when the virulence pattern of the fungus changes. In contrast, einkorn wheat shows prehaustorial resistance, which is not dependent on the specific race of the pathogen. By analyzing the genetic population of einkorn wheat, a major QTL associated with resistance was identified, and a specific gene involved in a hypersensitive defense reaction was found within this QTL interval.
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Plant Sciences
Angelina Malysheva, Alma Kokhmetova, Rakhym Urazaliev, Madina Kumarbayeva, Zhenis Keishilov, Makpal Nurzhuma, Ardak Bolatbekova, Assiya Kokhmetova
Summary: This study evaluated the resistance of 70 uncharacterized wheat cultivars and promising lines with unknown leaf rust resistance genes (Lr genes) to Kazakhstani Puccinia triticina (Pt) races. Thirteen wheat genotypes (18.6%) exhibited high resistance at both seedling and adult plant stages, with breeding materials from international nurseries showing higher resistance. Molecular screening identified nine Lr genes in 47 genotypes, with Lr37, Lr34, and Lr46 being the most common. These findings will facilitate wheat resistance breeding programs in Kazakhstan.
Article
Genetics & Heredity
Kassa Semagn, Muhammad Iqbal, Diego Jarquin, Jose Crossa, Reka Howard, Izabela Ciechanowska, Maria Antonia Henriquez, Harpinder Randhawa, Reem Aboukhaddour, Brent D. McCallum, Anita L. Brule-Babel, Alireza Navabi, Amidou N'Diaye, Curtis Pozniak, Dean Spaner
Summary: This study investigated the application of genomic selection in major diseases of spring wheat and compared the impact of different models on prediction accuracies. The results showed that the M3 model, which incorporated GE interactions, outperformed the M2 model in reducing residual variance and improving prediction accuracies.
Article
Plant Sciences
Karanjeet Singh Sandhu, Davinder Singh, Robert F. Park
Summary: Wheat is crucial for global food security with 2.5 billion people relying on it for dietary calories. Wheat stripe rust, a major constraint to production, is best controlled through genetic resistance. Developing a reliable assessment scale is essential for screening and characterizing rust resistance genes, pathogen survey, and fungicide research.
AUSTRALASIAN PLANT PATHOLOGY
(2022)
Article
Plant Sciences
Aurelie Quade, Robert F. Park, Ben J. Stodart, Gavin J. Ash
Summary: The specific pre- and post-cold storage treatments for maintaining the viability of rust fungi spores depend on the species. This study optimized the requirements for storing Puccinia sorghi spores and found that they remained infectious after 24 months of storage at -80 degrees C without the need for cold shock or heat shock.
CANADIAN JOURNAL OF PLANT PATHOLOGY
(2022)
Article
Microbiology
Michelle C. Moffitt, Johanna Wong-Bajracharya, Louise S. Shuey, Robert F. Park, Geoff S. Pegg, Jonathan M. Plett
Summary: In this study, untargeted metabolomics was used to explore the chemical defense profiles of different phenotypes within Melaleuca quinquenervia during the early stages of Austropuccinia psidii infection. The results identified different pools of secondary metabolites and provided an improved understanding of key pathways linked to rust resistance, particularly in Melaleuca. This study has significant implications for the management and conservation of Melaleuca species.
Article
Multidisciplinary Sciences
Hoan X. Dinh, Davinder Singh, Diana Gomez de la Cruz, Goetz Hensel, Jochen Kumlehn, Martin Mascher, Nils Stein, Dragan Perovic, Michael Ayliffe, Matthew J. Moscou, Robert F. Park, Mohammad Pourkheirandish
Summary: In this study, the researchers successfully isolated the barley leaf rust resistance gene Rph3 using positional cloning. They found that Rph3 encodes a unique transmembrane resistance protein and is expressed only upon infection by specific avirulent Puccinia hordei isolates. Heterologous expression of Rph3 induced a cell death response. The study also revealed limited genetic diversity in Rph3 in domesticated germplasm and higher diversity in wild barley.
NATURE COMMUNICATIONS
(2022)
Review
Agronomy
R. F. Park, W. H. P. Boshoff, A. L. Cabral, J. Chong, J. A. Martinelli, M. S. McMullen, J. W. Mitchell Fetch, E. Paczos-Grzeda, E. Prats, J. Roake, S. Sowa, L. Ziems, D. Singh
Summary: Crown rust, caused by Puccinia coronata f. sp. avenae (Pca), is a significant obstacle to oat production worldwide. Understanding the allelic relationships and chromosomal locations of resistance genes in oat is still limited. The high variability of Pca necessitates strategies to control the disease considering a large and diverse pathogen population. Pyramiding multiple resistance genes could enhance resistance levels.
THEORETICAL AND APPLIED GENETICS
(2022)
Editorial Material
Plant Sciences
Lin Huang, Yinghui Li, Shisheng Chen, Sambasivam Periyannan, Tzion Fahima
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Plant Sciences
Laura A. Ziems, Lovepreet Singh, Peter M. Dracatos, Mark J. Dieters, Miguel Sanchez-Garcia, Ahmed Amri, Ramesh Pal Singh Verma, Robert F. Park, Davinder Singh
Summary: A panel of 114 genetically diverse barley lines were assessed for resistance to Puccinia hordei. Several different resistance genes and genomic regions associated with resistance were identified. These findings provide valuable information for breeding efforts.
Article
Plant Sciences
Huy Chung Nguyen, Thi Tho Nguyen, Thi Nhung Lam, Thi Trang Le, Phuong Lan Le, Tien Hung Nguyen, Xuan Hoan Dinh
Summary: The rice bacterial blight caused by Xanthomonas oryzae pv. oryzae (Xoo) bacteria is the most destructive disease in rice. Studying the pathogenicity structure of the Xoo population helps propose the best breeding strategies using resistance genes. Among the 12 validated resistance genes, xa5 and Xa21 remained effective against the Xoo population in Vietnam, while other genes like Xa7 and xa13 were effective in certain regions. The most destructive Xoo races were found in the South, where intensive rice production was practiced, indicating pathogen migration between adjacent areas.
ARCHIVES OF PHYTOPATHOLOGY AND PLANT PROTECTION
(2023)
Article
Biochemistry & Molecular Biology
Md Arifuzzaman, Matthias Jost, Meinan Wang, Xianming Chen, Dragan Perovic, Robert F. Park, Matthew Rouse, Kerrie Forrest, Matthew Hayden, Ghazanfar Abbas Khan, Peter M. Dracatos
Summary: In this study, a genotype-by-sequencing approach was used to identify rust resistance alleles in a collection of diverse barley landraces and cultivars. Resistance factors were found on chromosomes 1H, 2H, 4H, and 5H. Additionally, a QTL on chromosome 5HL conferred resistance to both leaf rust and stripe rust.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Multidisciplinary Sciences
Chunhong Chen, Matthias Jost, Megan A. Outram, Dorian Friendship, Jian Chen, Aihua Wang, Sambasivam Periyannan, Jan Bartos, Katerina Holusova, Jaroslav Dolezel, Peng Zhang, Dhara Bhatt, Davinder Singh, Evans Lagudah, Robert F. Park, Peter M. Dracatos
Summary: In this study, the researchers successfully isolated the barley leaf rust resistance gene Rph7 using a fine mapping and RNA-Seq approach. They found that Rph7 encodes a non-canonical resistance protein that is structurally different from known plant disease resistance proteins. The study also revealed that Rph7 may mediate the activation and strength of the basal defence response, highlighting its importance for disease control in crops.
NATURE COMMUNICATIONS
(2023)
Editorial Material
Plant Sciences
Parimalan Rangan, Robert Henry, Peterson Wambugu, Sambasivam Periyannan
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Microbiology
Carolina Alessandra de Almeida Hayashibara, Mariana da Silva Lopes, Peri A. Tobias, Isaneli Batista dos Santos, Everthon Fernandes Figueredo, Jessica Aparecida Ferrarezi, Joao Paulo Rodrigues Marques, Joelma Marcon, Robert F. Park, Paulo Jose Pereira Lima Teixeira, Maria Carolina Quecine
Summary: Austropuccinia psidii is a biotrophic fungus that causes myrtle rust. It infects more than 480 myrtaceous species and has become a globally important pathogen. In this study, the researchers identified and characterized 255 virulence effector candidates in A. psidii strain MF-1 recovered from Eucalyptus grandis. They found that the expression of seven effector candidate genes is regulated by cell wax from resistant and susceptible hosts. This research provides new insights into the A. psidii-Eucalyptus interaction and offers a potential entry point for understanding how the pathogen manipulates its hosts using effector proteins.