Article
Chemistry, Analytical
Qiang Fu, Yunping Tu, Lun Cheng, Lulu Zhang, Xianbo Qiu
Summary: A fully-enclosed prototype 'pen' has been developed for rapid detection of SARS-CoV-2 using reverse transcriptase isothermal recombinase polymerase amplification (RT-RPA) and a dipstick assay. The handheld device, which includes amplification, detection, and sealing modules, allows for rapid nucleic acid amplification and detection in a fully enclosed environment. The detection 'pen' is enclosed to avoid aerosol contamination and the results can be directly observed with a colloidal gold strip-based detection.
SENSORS AND ACTUATORS B-CHEMICAL
(2023)
Review
Chemistry, Analytical
Saloni Kakkar, Payal Gupta, Navin Kumar, Krishna Kant
Summary: The detection of pathogens in food is crucial for public health and the environment. Nanomaterials have advantages over organic dyes in fluorescent-based detection methods due to their high sensitivity and selectivity. Microfluidic technology in biosensors has advanced to meet the criteria of sensitive, inexpensive, user-friendly, and quick detection.
Article
Veterinary Sciences
Oliver W. Stringer, Yanwen Li, Janine T. Bosse, Matthew S. Forrest, Juan Hernandez-Garcia, Alexander W. Tucker, Tiago Nunes, Francisco Costa, Preben Mortensen, Eduardo Velazquez, Paul Penny, Jesus Rodriguez-Manzano, Pantelis Georgiou, Paul R. Langford
Summary: Actinobacillus pleuropneumoniae (APP) is an important veterinary pathogen causing porcine pleuropneumoniae with high economic impact worldwide. Good biosecurity measures including regular diagnostic testing are essential. This study developed an isothermal point-of-care diagnostic test using recombinase polymerase amplification (RPA) and targeting the species-specific apxIVA gene, which achieved rapid and sensitive screening for APP.
FRONTIERS IN VETERINARY SCIENCE
(2022)
Article
Immunology
Dongdong Yin, Lei Yin, Jieru Wang, Xuehuai Shen, Xiaocheng Pan, Hongyan Hou, Ruihong Zhao, Xiaomiao Hu, Guijun Wang, Kezong Qi, Yin Dai
Summary: A convenient diagnostic method based on CRISPR/Cas13a was developed to detect DTMUV, which is sensitive, specific, and provides visual readout of the detection results. This method helps to reduce the occurrence of DTMUV disease.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Chemistry, Analytical
Julien Gomez-Martinez, Vincent Foulongne, Didier Laureillard, Nicolas Nagot, Brigitte Montes, Jean-Francois Cantaloube, Philippe Van de Perre, Chantal Fournier-Wirth, Jean-Pierre Moles, Jean-Charles Bres
Summary: HIV drug resistance poses a challenge to public health, highlighting the need for improved resistance testing. The rapid molecular method proposed in the study allows visual detection of HIV drug resistance mutations in a short timeframe, with higher sensitivity and specificity, suitable for multiplex detection.
Article
Microbiology
Anamaria D. Sarca, Luca Sardo, Hirofumi Fukuda, Hiroyuki Matsui, Kotaro Shirakawa, Kazuki Horikawa, Akifumi Takaori-Kondo, Taisuke Izumi
Summary: HIV-1 infectivity relies on virion maturation, with most proviruses in individuals on antiretroviral therapy having large deletions but preserving the ability to express viral structural proteins. A novel single-virion visualization technique using fluorescence resonance energy transfer allowed for the distinction between mature and immature virions based on their FRET signal. This tool can be used to assess maturation efficiency and understand the impact on immune responses of intact and defective viruses.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Rishabh Garg, Dinesh Prasad
Summary: Carbon dots (C-dots) have exceptional fluorescence and wavelength alteration capabilities, making them widely applicable in various fields. This review focuses on their applications in DNA detection technologies, as well as their interactions with metallic salts, dyes, and biological chemicals.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2023)
Article
Chemistry, Analytical
Kexin Ding, Yantian Shi, Yaojia Liu, Xinyue Song, Haiyan Wang, Jing Liu, Hong Zhou
Summary: We developed an enzyme-assisted multiple signal amplification reaction for target nucleic acid detection. This strategy involves utilizing the target nucleic acid as triggers, along with specific primers, DNA polymerase, and DNA nicking endonuclease, to promote repetitive cycle DNA replication and exponentially increase the nucleic acid sequence. The amplification products form a Mg2+-DNAzyme unit with the specific primer, enabling real-time fluorescence detection of HIV-1 DNA through cyclic cleavage of hairpin probes. Under optimal conditions, this method has a detection limit of 10-17 M. It offers a simple design, isothermal conditions, and easy fluorescence measurement, making it highly sensitive and specific for quantitative detection of DNA or RNA in theoretical and clinical studies.
SENSORS AND ACTUATORS B-CHEMICAL
(2023)
Article
Chemistry, Analytical
Xiaofen Zhao, Xiaoxiao Tian, Yuwei Wang, Linbin Li, Yan Yu, Shiqiao Zhao, Juan Zhang
Summary: An ultrasensitive and specific fluorescence method based on CRISPR-Cas12a-activated palindrome -catalytic hairpin assembly (CRISPR-Cas12a-PCHA) was developed for the detection of HIV-1 DNA. The method showed unique advantages of simple sample pretreatment, direct duplex target detection, and ultrahigh sensitivity, and could resist complex matrix interference and distinguish HIV patients from healthy individuals.
ANALYTICA CHIMICA ACTA
(2022)
Article
Microbiology
Lin Gong, Zhengjiang Jin, Ernan Liu, Fei Tang, Fengyun Yuan, Jiansheng Liang, Yimei Wang, Xiaoli Liu, Yi Wang
Summary: This study establishes a rapid and accurate diagnostic technology for detecting the mcr-1 gene, which provides a fast, sensitive, and specific method for monitoring mcr-1 in both pure isolates and clinical samples.
MICROBIOLOGY SPECTRUM
(2022)
Article
Biotechnology & Applied Microbiology
Rashid Aman, Tin Marsic, Gundra Sivakrishna Rao, Ahmed Mahas, Zahir Ali, Madain Alsanea, Ahmed Al-Qahtani, Fatimah Alhamlan, Magdy Mahfouz
Summary: This study presents a rapid and sensitive detection platform for mass-scale screening of SARS-CoV-2 in low-resource settings. The platform combines CRISPR/Cas gene editing technology with isothermal amplification methods to enable detection of SARS-CoV-2 at a single temperature in less than an hour. The authors also provide detailed guidelines for developing and establishing this one-pot assay, and the clinical validation shows high sensitivity and specificity.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Microbiology
Huaming Li, Xinyue Cao, Ruige Chen, Min Guang, Mengran Xu, Xiaomin Wu, Rongrong Yang, Liancheng Lei, Fuxian Zhang
Summary: In this study, a combined method of recombinant enzyme amplification (RPA) and CRISPR/Cas13a system was established to detect the vp7 gene of grass carp reovirus (GCRV). The method showed high sensitivity, specificity, and practicability for on-site detection of GCRV.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Chemistry, Analytical
Susana Campuzano, Maria Pedrero, Paloma Yanez-Sedeno, Jose M. Pingarron
Summary: There is a growing social demand for point of care testing (POCT) which is being facilitated by advancements in technology, widespread internet access, and the miniaturization of electrochemical sensors. These sensors, known for their adaptability to small devices and low cost, play a crucial role in meeting the main criteria for clinical POC devices, such as affordability, sensitivity, and ease of use.
SENSORS AND ACTUATORS B-CHEMICAL
(2021)
Article
Immunology
Kairui Wang, Jinfeng Wang, Cang Zhou, Xiaoxia Sun, Libing Liu, Xiangdong Xu, Jianchang Wang
Summary: In this study, a reverse transcription recombinase polymerase amplification (RT-RPA) assay targeting the ORF2 gene of hepatitis E virus (HEV) was developed. The assay integrated a fluorescence detection platform (qRT-RPA) and a visible lateral flow biosensor (LFB RT-RPA) for rapid and visual detection of HEV. The developed RT-RPA assays showed good specificity for HEV and demonstrated a high coincidence rate and Kappa value compared to the previously described qRT-PCR method. These results highlight the effectiveness of the RT-RPA assays as diagnostic tools for point-of-care detection of HEV in resource-limited settings.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Immunology
Ruiwen Li, Jinfeng Wang, Xiaoxia Sun, Libing Liu, Jianchang Wang, Wanzhe Yuan
Summary: This study developed and validated isothermal recombinase polymerase amplification (RPA) assays for quick and direct detection of Mycoplasma bovis in bovine milk. The RPA assays showed high specificity and efficiency in detecting M. bovis in milk samples, making them promising tools for rapid response to bovine mastitis caused by M. bovis infection.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2021)
Article
Gastroenterology & Hepatology
Yue Shen, Sheng-Di Wu, Yao Chen, Xin-Yue Li, Qin Zhu, Kiyoko Nakayama, Wan-Qin Zhang, Cheng-Zhao Weng, Jun Zhang, Hai-Kun Wang, Jian Wu, Wei Jiang
Summary: This study aimed to characterize the gut microbiota in patients with chronic hepatitis B virus infection-associated liver diseases (HBV-CLD) and evaluate their effects on peripheral immunity. The results revealed that disease progression and antiviral treatment were the two main factors contributing to the shift in microbiome and metabolome composition in HBV-CLD patients.
Article
Engineering, Electrical & Electronic
Guolin Yang, Xingliang Jiang, Jianlin Hu, Xiaodong Ren, Tao Li, Jianguo Wu, Zhijin Zhang, Qin Hu, Hualong Zheng
Summary: This study investigates the effects of particle concentration, particle size, and particle dielectric constant on the DC breakdown characteristics of unclean air gap. It is found that the presence of solid particles increases the breakdown voltage of the gap, with the increase being related to the particle concentration, size, and dielectric constant. Additionally, the presence of particles enhances both the ionization process and particle adsorption, leading to an increase in the gap breakdown voltage.
Article
Gastroenterology & Hepatology
Yu Song, Jiayi Wei, Rong Li, Ruifeng Fu, Pei Han, Heming Wang, Guangcong Zhang, Shuyu Li, Sinuo Chen, Zhiyong Liu, Yicheng Zhao, Changfeng Zhu, Jimin Zhu, Shuncai Zhang, Hao Pei, Jiefei Cheng, Jian Wu, Ling Dong, Guangqi Song, Xizhong Shen, Qunyan Yao
Summary: TrkB is decreased in liver fibrosis and its overexpression can inhibit the activation of TGF-beta/SMAD signaling and alleviate hepatic fibrosis, making it a potential therapeutic target.
Article
Computer Science, Artificial Intelligence
Chi Zhang, Jian Cui, Jianguo Wu, Xi Zhang
Summary: A steel plate surface defect detection method is proposed in this study, utilizing contextual information and attention mechanism, as well as a multi-layer feature extraction method and fusion framework based on low-level statistical textures. Through pixel-level spatial and correlation relationship identification, characteristics of low-level defects are extracted. A novel quantization technique is developed to effectively incorporate statistical texture in Convolutional Neural Networks (CNN). The empirical results demonstrate the feasibility of the proposed approach in practical steel plate testing and ablation experiments show that it enhances surface defect detection for steel plates.
JOURNAL OF INTELLIGENT MANUFACTURING
(2023)
Article
Thermodynamics
Alejandro Gallegos, Jianzhong Wu
Summary: The study aims to develop a predictive model for describing the electrostatic behavior of amino acids in aqueous solutions. A three-bead coarse-grained model is proposed, and a thermodynamic model accounting for nonideality effects is developed to capture how the interaction varies with solution conditions.
JOURNAL OF CHEMICAL AND ENGINEERING DATA
(2023)
Article
Engineering, Civil
Guanghui Zhao, Dongqi Wang, Taihu Sun, Yan Ding, Shu Chen, Yizhe Li, Hechen Sun, Chenyang Wu, Zhongjie Yu, Zhenlou Chen
Summary: Urban rivers are significant hotspots for greenhouse gas (GHG) emissions. This study investigated the impact of different rainfall events on GHG emissions from Suzhou Creek in Shanghai. It was found that typhoon events significantly increased GHG emissions, with factors such as rainfall dilution, pump station drainage, sediment disturbance, and high gas transfer coefficient playing important roles. The study highlights the importance of understanding the dynamic response of urban river GHG emissions to rainfall and typhoons.
JOURNAL OF HYDROLOGY
(2023)
Article
Chemistry, Physical
Debadutta Prusty, Alejandro Gallegos, Jianzhong Wu
Summary: The interfacial behavior of associating species is crucial for both fundamental physics and industrial applications. This study investigates the adsorption behavior of a mixture of nanoparticles and polymer chains, representing asphaltene and resin molecules, onto an attractive surface. The results provide insights into the effects of various characteristics of these molecules on the adsorption amount and reveal the interplay between different driving forces.
JOURNAL OF MOLECULAR LIQUIDS
(2023)
Article
Chemistry, Analytical
Yijing Wu, Wenjun Zheng, Canrong Chen, Linyan Yang, Ping Tong, Yanhui Zhong, Zian Lin, Zongwei Cai
Summary: In this study, a spherical covalent organic framework (COf) based on imine linkages was prepared and utilized as an adsorbent in dispersive solid-phase extraction. The dSPE-HPLC-MS/MS method based on the spherical COF demonstrated high efficiency and accuracy in the determination of aryl organophosphate esters in biological samples.
JOURNAL OF SEPARATION SCIENCE
(2023)
Article
Materials Science, Coatings & Films
Yinchun Fang, Jianguo Wu, Shuo Chang, Lingshuang Wu
Summary: This study used bio-based phytic acid (PA) and gelatine (G) to improve the flame retardancy and anti-dripping properties of polyethylene terephthalate (PET) fabric. The coated PET fabric showed a limiting oxygen index (LOI) value greater than 29.5% and no dripping phenomenon, indicating significant improvement in flame retardancy and anti-dripping properties. The PA/G coating altered the decomposition stability and degradation process of PET fabric, promoting the formation of a stable char. The coating formed an intumescent flame retardant (IFR) system on PET fabric, functioning through the IFR mechanism.
SURFACE ENGINEERING
(2023)
Article
Virology
Weijie Chen, Yukun Li, Xiuling Yu, Zhenwei Wang, Wenbiao Wang, Menglan Rao, Yongkui Li, Zhen Luo, Qiwei Zhang, Jinbiao Liu, Jianguo Wu
Summary: In this study, it was found that Zika virus infection upregulated 7-dehydrocholesterol reductase (DHCR7) expression, which in turn facilitated viral infection. The nonstructural protein NS4B of Zika virus interacted with DHCR7 to induce its expression, thereby inhibiting the immune response and facilitating viral evasion. This study expands our understanding of the mechanisms by which viral nonstructural proteins antagonize innate immunity during viral infection via cholesterol metabolic enzymes and intermediates.
Article
Automation & Control Systems
Zhiyong Hu, Chao Wang, Jianguo Wu, Dongping Du
Summary: The development of sensor technologies has allowed the acquisition of high dimensional sensing data, which has led to the development of multi-output modeling techniques for decision making. However, missing values in the data often result in information loss and affect the modeling performance. This study proposes a new multi-output modeling method based on GPLVM to exploit useful information in the data and improve modeling accuracy. The method demonstrates superior performance in both simulation and case studies, making it a powerful tool for process automation.
IEEE TRANSACTIONS ON AUTOMATION SCIENCE AND ENGINEERING
(2023)
Review
Biochemistry & Molecular Biology
Hui Chen, Cheng Jin, Li Xie, Jian Wu
Summary: Succinate, an intermediate of the TCA cycle, is crucial in mitochondrial metabolism, energy production, and is also considered a signaling molecule in metabolism and hepatic diseases. Its downstream signaling pathway through GPR91 leads to various intracellular responses, including succinylation, ROS production, HIF-1 alpha stabilization, and significant impact in cellular metabolism due to its role in the TCA cycle. Understanding the signaling mechanisms of succinate in hepatic fibrosis, metabolic reprogramming, inflammatory or immune responses, and carcinogenesis is of great interest. This review aims to provide the current understanding of succinate in mediating metabolism, inflammatory and immune responses in liver diseases, with the aim of establishing a molecular basis for therapeutic strategies.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE
(2024)
Article
Medicine, Research & Experimental
Yuan Zhou, Li Zhang, Yue Ma, Li Xie, Yong-Yu Yang, Cheng Jin, Hui Chen, Ying Zhou, Guang-Qi Song, Jia Ding, Jian Wu
Summary: This study found that senescent hepatic stellate cells (HSCs) play a crucial role in the development of nonalcoholic steatohepatitis (NASH) and hepatocellular carcinoma (HCC). Senescent HSCs secrete proteins that activate morphogenic hedgehog or oncogenic Wnt signaling pathways, promoting the malignant transformation of steatotic or dysplastic hepatocytes.
Article
Mathematics, Applied
Marco Tosato, Xue Zhang, Jianhong Wu
Summary: We investigate the behavior of solutions to a scalar Delay Differential Equation (DDE) with periodic switching between two constant values. This equation naturally arises in structured vector populations involved in the spread of various vector-borne diseases in a periodically changing environment. We examine the influence of two different time lags and the switching time on the existence and patterns of periodic solutions, with a specific focus on multi-cycles within the prime period.
DIFFERENTIAL EQUATIONS AND DYNAMICAL SYSTEMS
(2023)
Article
Chemistry, Analytical
Qian Wu, Li Tian, Xiangyu Shan, Huiling Li, Chao Li, Juan Lu
Summary: A novel ECL sensor was developed by modifying Fe3CuO4-Ru(bpy)3+ and GO-CdS@ZnS on the electrode for the detection of permethrin. The sensor exhibited high sensitivity and a wide detection range.
Article
Chemistry, Analytical
Tingting Gong, Lei Liao, Bingying Jiang, Ruo Yuan, Yun Xiang
Summary: In this study, a fluorescent biosensing system for high sensitivity detection of MT was established using a new Ag+-stabilized DNA triplex probe (Ag+-SDTP) combined with catalytic hairpin assembly (CHA) and CRISPR/Cas12a signal enhancements. The sensing method is able to selectively detect MT from other non-specific molecules and can achieve low level detection of MT in diluted human serums, demonstrating its potential for monitoring disease-specific MT biomarker at trace levels.
Article
Chemistry, Analytical
Estrella Sanz Rodriguez, Kyra Wood, Paul R. Haddad, Brett Paull
Summary: This study presents a simple, rapid, and reliable method for determining boron in aqueous solutions using suppressed ion chromatography coupled to electrospray ionisation-triple quadrupole mass spectrometry. The method achieved a low limit of detection and demonstrated excellent analytical performance.
Article
Chemistry, Analytical
Aline Cournut, Paul Moustiez, Yannick Coffinier, Christine Enjalbal, Claudia Bich
Summary: Alzheimer's disease is a major cause of senile dementia and is characterized by the aggregation of neurofibrillary tangles and amyloid plaques in the brain. The A beta 1-42 peptide is considered the primary cause of neurotoxicity due to its insolubility and subsequent aggregation. A beta 1-42 and A beta 1-40 have been identified as relevant biomarkers for AD diagnosis, and SurfaceAssisted Laser Desorption/Ionization Mass Spectrometry (SALDI-MS) was used to monitor the A beta 1-42/A beta 1-40 ratio without prior sample treatment.
Article
Chemistry, Analytical
Dotse Selali Chormey, Buse Tugba Zaman, Tulay Borahan Kustanto, Sezin Erarpat Bodur, Suleyman Bodur, Elif Ozturk Er, Sezgin Bakirdere
Summary: This review provides an overview of the harmful effects of endocrine disrupting chemicals (EDCs) on human health and introduces the methods for analyzing EDCs. Recent research has focused on using green chemicals, such as deep eutectic solvents (DESs), for microextraction to ensure environmental safety.
Article
Chemistry, Analytical
Yan Zhang, Renfeng Xu, Jingjing Wu, Zhenghong Zhang, Yuhuang Wang, Hongqin Yang, Sheng Zhang
Summary: This study visualized the pore-like structures in prostate cancer cell membranes after treatment with the anticancer drug paclitaxel, demonstrating its mechanism of action related to oxidative damage.
Article
Chemistry, Analytical
Chihong Ma, Qin Zhou, Jinjin Shi, Hua Gao, Di Huang, Huimin Xue, Han Wang, Zhenzhong Zhang, Sen Yang, Junli Zhang, Kaixiang Zhang
Summary: We developed a CRISPR-empowered electrochemical biosensor, named PER-E-CRISPR, for amplification-free and sensitive detection of miR-21. The biosensor combines the advantages of CRISPR/Cas13a and primer exchange reaction (PER) for target amplification. Under optimized conditions, the PER-E-CRISPR assay exhibits a linear range from 10-13 to 10-7 M and a limit of detection of 30.2 fM. The biosensor shows excellent performance in actual plasma, indicating promising prospects for miRNA detection in molecular diagnosis.
Article
Chemistry, Analytical
Gang Liang, Jie Zhao, Yufei Gao, Tao Xie, Jianhui Zhen, Ligang Pan, Wenwen Gong
Summary: Molecular docking (MD) analysis is widely used for studying the interaction between aptamers and small molecules. This study established steady-state aptamers of tertiary structures (SATS) using specific aptamers of tetracycline antibiotics as docking models. Molecular docking results revealed multiple binding sites in the SATS of aptamers, with significant variations in binding free energy (BFE) and docking score (DS). The study also proposed a method for MD analysis based on SATS, providing insights into binding mode and predicting binding sites.
Article
Chemistry, Analytical
Yixuan Xu, Changchang Teng, Huiping Dang, Dalong Yin, Lifeng Yan
Summary: Stable organic radical molecules with high NIR fluorescence quantum efficiency have been synthesized and encapsulated into nanoparticles, which show great potential for both photodynamic therapy (PDT) and photothermal therapy (PTT) of tumors.
Article
Chemistry, Analytical
Yu Su, Dandan Yang, Yanjie Wang, Jie Ding, Lan Ding, Daqian Song
Summary: In this study, a highly selective molecularly imprinted polymer Cu-MIPs@CS was designed and synthesized for bisphenol A (BPA). Cu-MIPs@CS showed significantly enhanced imprinting factor and selective factor towards BPA compared to MIPs@CS. It also exhibited superior discrimination ability between BPA and its structural analogue. Additionally, Cu-MIPs@CS were successfully applied as a solid phase extraction adsorbent for the determination of BPA in drinking water samples.
Article
Chemistry, Analytical
Yi Qiao, Qiongdan Zhang, Yukun He, Tianguang Cheng, Jing Tu
Summary: Single cell heterogeneity plays an important role in many biological phenomena, and distinguishing cells with specific mutations is beneficial for clinical diagnosis and drug screening. This study introduces a gel plate platform that can distinguish cell types based on their phenotypes, with low equipment requirement. The gel platform has potential in point-of-care circumstances and single-cell stimulation responses that prioritize efficiency and simplicity.
Article
Chemistry, Analytical
Chengke Wang, Shuyang Sun, Ping Wang, Huawei Zhao, Wenling Li
Summary: The food safety of aquatic products is a global concern. Nanotechnology-based analyses have advantages in the detection of bacteria, metal ions, and small molecule contaminants. This review summarizes the recent advances in biosensing strategies for aquatic products and highlights the application of nanomaterials, lateral flow-based biosensors, surface-enhanced Raman scattering, microfluidic chips, and molecular imprinting technologies.
Article
Chemistry, Analytical
Liping Zhu, Zeng Tang, Xuemei Zhang, Li Zhu, Tian Meng, Linying Yu, Ting Xiao, Shasha Lu, Xiaoli Xiong, Xiurong Yang
Summary: A sensitive off-on electrochemiluminescence DNA sensor was developed using Exo III-assisted cascade amplification system. The sensor achieved high selectivity and ultra-high sensitivity in detecting target DNA by utilizing ECL and SPR effects. This study provides a new perspective for designing highly sensitive and programmable ECL biosensors.
Article
Chemistry, Analytical
Tong Li, Mengxu Sun, Suping Xia, Ting Huang, Rong-Tian Li, Chunrong Li, Zong Dai, Jin-Xiang Chen, Jun Chen, Nuan Jia
Summary: Here, we developed a binary system based on DNA tetrahedron and fluorogenic RNA aptamers for highly specific and label-free mRNA imaging in living cells. This system achieved high specificity label-free detection of nucleic acids with a detection limit of 1.34 nM. The method has potential applications in early disease diagnosis and new drug development.
Article
Chemistry, Analytical
Yao Zhao, Shu Zhang, Wang Yao, Yuxuan Zhu, Jing Qian, Juan Yang, Nianjun Yang
Summary: A new hierarchical nanostructured composite with high conductivity and catalytic activity is synthesized. The sensor exhibits significant catalytic effect and high sensitivity for monitoring the environmental endocrine disruptor bisphenol A (BPA).