Article
Plant Sciences
Irene Bosmali, Georgios Lagiotis, Nadia Haider, Maslin Osathanunkul, Costas Biliaderis, Panagiotis Madesis
Summary: By analyzing DNA barcoding regions, this study identified 71 Vicia specimens of Eurasian descent. The trnL and ITS2 regions were effective in discriminating the analyzed species, while the rpoC1 region could not identify all selected species due to sequence conservation or missing sequences in GenBank. The combination of DNA barcoding regions is essential for accurate species discrimination within the Vicia genus.
Article
Plant Sciences
Jianli Wang, Zhenfei Yan, Peng Zhong, Zhongbao Shen, Guofeng Yang, Lichao Ma
Summary: This study evaluated the utility of four candidate DNA barcoding regions in seven genera of Gramineae. It found that matK and ITS showed the highest discriminatory power, while rbcL and trnL-F were less effective. The study also demonstrated the feasibility of establishing a technical system for rapid and reliable identification of these seven genera, and provided crucial reference for analyzing the genetic diversity of grass germplasm resources.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Microbiology
Renato Daniel La Torre, Daniel Ramos, Mayra Doris Mejia, Edgar Neyra, Edwin Loarte, Gisella Orjeda
Summary: DNA barcoding is effective for identifying well-represented lichenized fungi groups, but its effectiveness is limited in understudied regions. A study on King George Island in Antarctica used a fungal barcode marker to survey lichenized fungi diversity. The majority of samples were accurately identified using the barcode marker, and subsequent morphological evaluations led to the identification of unknown species. These results contribute to the understanding of lichenized fungi diversity in understudied regions and provide valuable guidance for taxonomy efforts in species recognition and discovery.
Article
Biology
Amrita Srivathsan, Leshon Lee, Kazutaka Katoh, Emily Hartop, Sujatha Narayanan Kutty, Johnathan Wong, Darren Yeo, Rudolf Meier
Summary: This study introduces a cost-effective and simple DNA barcoding method that can be used in various settings. The research provides amplicon coverage recommendations based on multiple runs of the latest generation of MinION flow cells, and introduces a novel software to address bioinformatics challenges related to MinION reads. Additionally, the study suggests that MinION barcodes are highly similar to Sanger and Illumina barcodes, and that MinION technology has improved rapidly since 2018.
Article
Plant Sciences
Girme Aoudumbar Ramesh, Deepu Mathew, K. Joseph John, V Ravisankar
Summary: This study utilized DNA barcoding to identify seven species of Momordica and determine their relationships. Analysis of matK gene sequences resulted in distinct barcodes that could differentiate the species. The study also confirmed M. cymbalaria as a member of the Momordica genus.
HORTICULTURAL PLANT JOURNAL
(2022)
Article
Entomology
Zhuo Ma, Jinliang Ren, Runzhi Zhang
Summary: This study analyzed the genetic distance variation of species in the subfamily Entiminae to determine parameters for species identification. The results showed that there is no universal genetic distance threshold at the subfamily level, but a threshold of 9.18% can delimit more than 88% of Entiminae species.
Article
Plant Sciences
Zhi-Fang Liu, Hui Ma, Xiao-Yan Zhang, Xiu-Qin Ci, Lang Li, Jian-Lin Hu, Can-Yu Zhang, Jian-Hua Xiao, His-Wen Li, John G. Conran, Alex D. Twyford, Peter M. Hollingsworth, Jie Li
Summary: The aim of DNA barcoding is to accurately identify species. However, universal barcodes often fail to discriminate species in taxonomically complex groups. The development of assays that allow low-cost access to large numbers of nuclear markers is a major challenge in plant DNA barcoding.
BOTANICAL JOURNAL OF THE LINNEAN SOCIETY
(2022)
Article
Entomology
Chao Song, Le Wang, Teng Lei, Xin Qi
Summary: Through analysis of collected species and public sequences, it is found that DNA barcodes can successfully delimit Microtendipes species and there are deep intraspecific divergences in some species. Additionally, color patterns are confirmed to be important diagnostic characters. Therefore, this study identifies and describes five new species and provides an updated key to male adults of known Microtendipes species from China.
Article
Chemistry, Applied
Nan Wang, Ran-Ran Xing, Meng-Yue Zhou, Rui-Xue Sun, Jian-Xun Han, Jiu-Kai Zhang, Wen-Jie Zheng, Ying Chen
Summary: This study tested the feasibility of next-generation sequencing in identifying mixed salmon products and found that 50% of the commercial salmon food products surveyed were mislabelled. DNA barcoding and metabarcoding methods are useful for the identification of salmon species in food and can be used for quality control of various types of salmon products.
FOOD ADDITIVES AND CONTAMINANTS PART A-CHEMISTRY ANALYSIS CONTROL EXPOSURE & RISK ASSESSMENT
(2021)
Article
Biodiversity Conservation
Huiqing Yeo, Tze Xuan Yeoh, Huicong Ding, Theodore Tze Ming Lee, Nalini Puniamoorthy
Summary: This study utilized an integrative approach of morphological identification and molecular delineation with mini-barcodes to estimate species of mosquitoes in Singapore. The full dataset provided a more defined and accurate community structure across all sites compared to using only adult data, highlighting the importance of including larval data in mosquito ecological analyses. It was recommended to use both morphological and molecular identification methods for ecological studies and vector surveillance to avoid misidentification and ensure robust datasets for downstream analyses.
JOURNAL OF APPLIED ECOLOGY
(2021)
Article
Food Science & Technology
Jiahleen B. Roungchun, Amanda M. Tabb, Rosalee S. Hellberg
Summary: Accurate species identification of tuna products is crucial for combating fraud, improving stock regulation, and reducing health risks. This study utilized a DNA mini-barcoding system to successfully identify tuna species in raw and processed samples, with potential for further optimization in future research for canned tuna samples.
Article
Ecology
Karen L. Bell, Robert A. Petit, Anya Cutler, Emily K. Dobbs, J. Michael Macpherson, Timothy D. Read, Kevin S. Burgess, Berry J. Brosi
Summary: In this study, the performance of whole-genome shotgun (WGS) sequencing for species identification and quantitative analysis of pollen was evaluated. WGS showed near 100% accuracy in species identification in mixtures but generated higher rates of false positives compared to rbcL and ITS2 amplicon sequencing. WGS data provided a stronger correlation between pollen grain proportion and sequence read proportion for relative species abundance quantification, but diverged more from a 1:1 relationship.
ECOLOGY AND EVOLUTION
(2021)
Article
Pharmacology & Pharmacy
Jinxin Liu, Weishan Mu, Mengmeng Shi, Qing Zhao, Weijun Kong, Hongbo Xie, Linchun Shi
Summary: This study utilized shotgun metabarcoding to identify the biological ingredients in traditional herbal patent medicine Wuhu San. The results showed that this technology could effectively complement traditional methods in identifying prescribed ingredients.
FRONTIERS IN PHARMACOLOGY
(2021)
Article
Plant Sciences
Shuai Jiang, Fenglin Chen, Pai Qin, Hai Xie, Guo Peng, Yongliang Li, Xinhong Guo
Summary: The study focuses on the accurate identification of Theaceae plants using DNA barcoding technology. The researchers identified the optimal combination of gene sequences for DNA barcodes and conducted phylogenetic analysis and species-specific barcode design. The results improve species identification accuracy and have important implications for the identification and protection of Theaceae species and germplasm resources.
PHYSIOLOGY AND MOLECULAR BIOLOGY OF PLANTS
(2022)
Article
Biochemistry & Molecular Biology
Isabel C. Kilian, Stephanie J. Swenson, Ximo Mengual, Birgit Gemeinholzer, Andree Hamm, J. Wolfgang Waegele, Ralph S. Peters
Summary: This study investigated the plant-pollinator network of caraway in a central European agricultural landscape, focusing on two potential pollinator taxa: Diptera and Hymenoptera. The results showed that both Diptera and Hymenoptera can carry caraway pollen, indicating a wide taxonomic breadth of potential pollinators and a higher network complexity than previously anticipated. The study also found that there are distinct qualitative differences between the networks of the two taxa, suggesting complementary roles of both taxa in pollination.
Article
Infectious Diseases
Mackenzie L. Kwak, Erica S. Neves, Sophie A. Borthwick, Gavin J. D. Smith, Rudolf Meier, Ian H. Mendenhall
Summary: The study found that old secondary forests in Singapore have a significant impact on both the abundance and structure of tick communities on small mammals, and are the preferred habitat of the zoonotic tick Ixodes granulatus. Therefore, future disease spillover is likely to emerge from small mammal-tick communities in old secondary forests.
TICKS AND TICK-BORNE DISEASES
(2021)
Article
Biology
Keith M. Bayless, Michelle D. Trautwein, Karen Meusemann, Seunggwan Shin, Malte Petersen, Alexander Donath, Lars Podsiadlowski, Christoph Mayer, Oliver Niehuis, Ralph S. Peters, Rudolf Meier, Sujatha Narayanan Kutty, Shanlin Liu, Xin Zhou, Bernhard Misof, David K. Yeates, Brian M. Wiegmann
Summary: This study utilized transcriptome data to investigate the phylogenetic relationships of schizophoran flies, supporting the division of most acalyptrate fly groups into four major lineages consistently across analyses. The research revealed the primary branching patterns and highlighted important sister group relationships, laying a foundation for future comparative research.
Article
Biology
Cengiz Kaya, Tomas N. Generalovic, Gunilla Stahls, Martin Hauser, Ana C. Samayoa, Carlos G. Nunes-Silva, Heather Roxburgh, Jens Wohlfahrt, Ebenezer A. Ewusie, Marc Kenis, Yupa Hanboonsong, Jesus Orozco, Nancy Carrejo, Satoshi Nakamura, Laura Gasco, Santos Rojo, Chrysantus M. Tanga, Rudolf Meier, Clint Rhode, Christine J. Picard, Chris D. Jiggins, Florian Leiber, Jeffery K. Tomberlin, Martin Hasselmann, Wolf U. Blanckenhorn, Martin Kapun, Christoph Sandrock
Summary: The study provides the first comprehensive genetic characterization of black soldier fly populations, revealing 16 well-distinguished genetic clusters with significant global population structure. It highlights the dynamic population genetic history and ongoing domestication of black soldier flies, with implications for future research on this emerging insect-livestock model.
Article
Biology
Darren Yeo, Amrita Srivathsan, Jayanthi Puniamoorthy, Foo Maosheng, Patrick Grootaert, Lena Chan, Benoit Guenard, Claas Damken, Rodzay A. Wahab, Ang Yuchen, Rudolf Meier
Summary: The study reveals that mangroves are an overlooked hotspot for insect diversity, with a highly distinct and species-rich insect fauna. Despite a comparatively low number of phytophagous and fungivorous insect species, mangroves compensate by supporting a rich community of predators. The diversity patterns of insect communities in other tropical habitats are largely congruent across guilds.
Article
Evolutionary Biology
Rudolf Meier, Bonnie B. Blaimer, Eliana Buenaventura, Emily Hartop, Thomas Rintelen, Amrita Srivathsan, Darren Yeo
Summary: Halting biodiversity decline is crucial but hindered by taxonomic impediments, including undescribed and superficially described species. A recent study attempted to address the issue by using COI barcode clusters for braconid wasps in Costa Rica, but the accuracy of the descriptions is questionable.
Article
Biology
Liping Yan, Thomas Pape, Karen Meusemann, Sujatha Narayanan Kutty, Rudolf Meier, Keith M. Bayless, Dong Zhang
Summary: This study provides the first phylogeny of oestroid calyptrates including all blowfly subfamilies, settling a long-lasting controversy in Diptera by redefining blowflies as a well-supported monophylum. The archetypical blowfly trait of carrion-feeding maggots most likely evolved twice, and the metallic color may not belong to the blowfly ground plan.
Article
Biology
Amrita Srivathsan, Leshon Lee, Kazutaka Katoh, Emily Hartop, Sujatha Narayanan Kutty, Johnathan Wong, Darren Yeo, Rudolf Meier
Summary: This study introduces a cost-effective and simple DNA barcoding method that can be used in various settings. The research provides amplicon coverage recommendations based on multiple runs of the latest generation of MinION flow cells, and introduces a novel software to address bioinformatics challenges related to MinION reads. Additionally, the study suggests that MinION barcodes are highly similar to Sanger and Illumina barcodes, and that MinION technology has improved rapidly since 2018.
Article
Biochemistry & Molecular Biology
Lorenz Wuehrl, Christian Pylatiuk, Matthias Giersch, Florian Lapp, Thomas von Rintelen, Michael Balke, Stefan Schmidt, Pierfilippo Cerretti, Rudolf Meier
Summary: Invertebrate biodiversity remains poorly understood, with challenges in sampling and classification. A sorting robot is proposed in this study to prepare specimens for DNA barcoding and train neural networks to assign specimens to common taxa.
MOLECULAR ECOLOGY RESOURCES
(2022)
Article
Evolutionary Biology
Leshon Lee, David J. X. Tan, Jozef Obona, Daniel R. Gustafsson, Yuchen Ang, Rudolf Meier
Summary: This study illustrates how new natural history data on phoretic relationships can be obtained quickly using modern and cost-effective barcoding techniques. The authors developed a protocol based on screening bird carcasses to generate new records of avian lice riding on hippoboscid flies. They argue that large-scale NGS barcoding can help resolve many phoretic relationships between arthropods.
SYSTEMATIC ENTOMOLOGY
(2022)
Article
Evolutionary Biology
Emily Hartop, Amrita Srivathsan, Fredrik Ronquist, Rudolf Meier
Summary: New method called large-scale integrative taxonomy (LIT) is proposed for tackling dark taxa, which are groups with a low percentage of described species and a high estimated diversity. LIT generates preliminary species hypotheses based on inexpensive data, which are then evaluated using more expensive validation data. In this study, LIT was used to sort scuttle flies into preliminary species hypotheses based on DNA barcodes, which were then validated using morphological data. Quantitative indicators for predicting incongruence between barcodes and morphology were developed. The results showed that LIT can effectively and efficiently delimit species.
SYSTEMATIC BIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Yin Cheong Aden Ip, Jia Jin Marc Chang, Karenne Phyu Phyu Tun, Rudolf Meier, Danwei Huang
Summary: This study uses environmental DNA metabarcoding to monitor two tropical reef sites and shows that during coral spawning, there is an increase in eDNA coral signals, which can detect a large number of coral and fish species.
Editorial Material
Biochemistry & Molecular Biology
Brent C. Emerson, Paulo A. V. Borges, Pedro Cardoso, Peter Convey, Jeremy R. deWaard, Evan P. Economo, Rosemary G. Gillespie, Susan Kennedy, Henrik Krehenwinkel, Rudolf Meier, George K. Roderick, Dominique Strasberg, Christophe Thebaud, Anna Traveset, Thomas J. Creedy, Emmanouil Meramveliotakis, Victor Noguerales, Isaac Overcast, Helene Morlon, Anna Papadopoulou, Alfried P. Vogler, Paula Arribas, Carmelo Andujar
Summary: The current understanding of the ecological and evolutionary processes underlying island biodiversity is largely based on empirical data from plants and birds, overlooking the fact that arthropods constitute the majority of known animal species and can offer crucial insights into biodiversity processes. High throughput sequencing approaches are emerging as powerful tools for overcoming limitations in arthropod biodiversity data, and can provide valuable insights into these processes. This article explores the effective utilization of these tools for comprehensive and comparable inventory and monitoring of arthropod biodiversity on islands.
Article
Biochemistry & Molecular Biology
Amrita Srivathsan, Rebecca Ker Loh, Elliott James Ong, Leshon Lee, Yuchen Ang, Sujatha Narayanan Kutty, Rudolf Meier
Summary: DNA obtained from invertebrates can be used to survey vertebrate communities, but the interaction between the two groups is not well understood. This study found that there was no specialization in the interactions between the dung and carrion fly community and the vertebrate species. However, uncommon fly species can carry the DNA signal for vertebrate species that are difficult to detect using iDNA. The fly community sourced for iDNA was unexpectedly rich and carried DNA for a variety of rare and common vertebrate species.
Article
Ecology
Amrita Srivathsan, Yuchen Ang, John M. M. Heraty, Wei Song Hwang, Wan F. A. Jusoh, Sujatha Narayanan Kutty, Jayanthi Puniamoorthy, Darren Yeo, Tomas Roslin, Rudolf Meier
Summary: Using DNA barcoding, the authors analyzed the diversity of flying insects and found that more than half of the local species diversity is represented by only 20 insect families, most of which have been neglected in terms of taxonomy. The dominance of these families in insect diversity remains consistent across various factors such as clade age, continent, climatic region, and habitat type. These families, however, suffer from taxonomic neglect and urgent measures are needed to address this issue in biodiversity science.
NATURE ECOLOGY & EVOLUTION
(2023)
Article
Multidisciplinary Sciences
Connor M. French, Laura D. Bertola, Ana C. Carnaval, Evan P. Economo, Jamie M. Kass, David J. Lohman, Katharine A. Marske, Rudolf Meier, Isaac Overcast, Andrew J. Rominger, Phillip P. A. Staniczenko, Michael J. Hickerson
Summary: Understanding global patterns of genetic diversity is crucial for studying and preserving biodiversity. This study creates a global map of predicted insect mitochondrial genetic diversity and identifies the environmental factors associated with genetic diversity. The findings contribute to our understanding of global biodiversity patterns in the most diverse animal group and have important implications for conservation efforts.
NATURE COMMUNICATIONS
(2023)