Article
Biochemical Research Methods
Zhi-Ying Cui, Chun-Lu Liu, Dan-Dan Li, Yuan-Zhong Wang, Fu-Rong Xu
Summary: This study aimed to analyze the correlation of components and activities of P. notoginseng from different origins and develop a rapid identification method. Pharmacological experiments, HPLC, and spectroscopy combined with chemometrics methods were conducted to analyze the main roots of P. notoginseng from four different origins in Yunnan Province. The results showed that the saponin content of P. notoginseng main roots was not significantly different, but the anticoagulant activity was positively correlated with the contents of the three saponins. The variable selection method improved the performance of the model, and the variable importance in projection had the best ability to identify the origins of P. notoginseng.
PHYTOCHEMICAL ANALYSIS
(2022)
Article
Biochemical Research Methods
Zhouqian Jiang, Haiyun Gao, Rong Liu, Meng Xia, Yun Lu, Jiadian Wang, Xiaochao Chen, Yifeng Zhang, Dan Li, Yuru Tong, Panting Liu, Yuan Liu, Yunfeng Luo, Jie Gao, Yan Yin, Luqi Huang, Wei Gao
Summary: In this study, candidate glycosyltransferase genes were screened based on the previous genome and transcriptome data of Panax notoginseng, and the full length of 27 UGT genes were successfully cloned. Among them, it was found that the PnUGT33 gene could catalyze different ginsenoside substrates to produce higher polarity rare ginsenosides. The enzymatic kinetics of PnUGT33 were analyzed and the catalytic mechanism was predicted through molecular docking simulation. Ultimately, the biosynthetic pathway of rare ginsenoside Rg(3) and gypenoside LXXV was reconstructed in yeast, and the yields were significantly improved by overexpressing UDPG biosynthetic genes.
ACS SYNTHETIC BIOLOGY
(2022)
Article
Biochemical Research Methods
Zhouqian Jiang, Haiyun Gao, Rong Liu, Meng Xia, Yun Lu, Jiadian Wang, Xiaochao Chen, Yifeng Zhang, Dan Li, Yuru Tong, Panting Liu, Yuan Liu, Yunfeng Luo, Jie Gao, Yan Yin, Luqi Huang, Wei Gao
Summary: In this study, 27 glycosyltransferase genes were screened and PnUGT33 was found to catalyze the production of rare ginsenosides. The researchers further analyzed the catalytic mechanism of PnUGT33 and reconstructed the biosynthetic pathway of rare ginsenosides in yeast, successfully improving the yield.
ACS SYNTHETIC BIOLOGY
(2022)
Article
Biochemical Research Methods
Qinghai Dong, Yang An, Guangguang Du, Jia Wang, Jiayin Liu, Jun Su, Hongliu Xie, Chongyang Liang, Jihua Liu
Summary: In this study, the differential bioactivities of Panax notoginseng (PN) and Panax ginseng (PG) were investigated using a metabolomics approach. The results revealed the differences in metabolites and signaling pathways that contribute to the distinct bioactivities of PN and PG.
BIOMEDICAL CHROMATOGRAPHY
(2022)
Article
Food Science & Technology
Hui-Rong Yang, Xue Li, Hao Liu, Dong-Rui Zhao, Ying-Jie Zeng
Summary: In this study, a polysaccharide Pnp was prepared from P. notoginseng, and its structure and biology activities were investigated. Results showed that Pnp mainly consisted of -*2,4)-alpha-L-Rhap- (1-*,-*5)-alpha-L-Araf-(1-* and-*4)-alpha-D-Galp-(1-* residues with acetyl groups. Furthermore, Pnp activated the immunologic function of macrophages and could be utilized by the probiotic Lactobacillus plantarum, enhancing its hydrophobicity and the level of short-chain fatty acids. These findings suggest that Pnp has potential as a nutraceutical and functional candidate in the food industry.
Article
Agriculture, Multidisciplinary
Maoqi Hou, Feng Nie, Jianing Zhao, Zhengcai Ju, Li Yang, Qingzhong Wang, Shujuan Zhao, Zhengtao Wang
Summary: In this study, six UGTs were characterized, two of which were novel. PnUGT71A3 catalyzes the glycosylation of protopanaxatriol (PPT) and F1 to form Rh1 and Rg1, respectively, and also the glycosylation of protopanaxadiol (PPD)-type Rg3 to generate Rd. PnUGT94M1 regioselectively catalyzes the rhamnosylation of the C6 glucose of PPT-type ginsenosides Rg1 and Rh1, generating ginsenosides Re and Rg2, respectively, and is UDP-beta-L-rhamnose (UDP-Rha)-dependent.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2023)
Article
Pharmacology & Pharmacy
Junyun Cheng, Jie Chen, Jie Liao, Tianhao Wang, Xin Shao, Jinbo Long, Penghui Yang, Anyao Li, Zheng Wang, Xiaoyan Lu, Xiaohui Fan
Summary: A new approach called TCM-seq was developed to comprehensively evaluate the therapeutic effects of Panax ginseng and Panax notoginseng at the transcriptomic level. The study revealed significant differences in the regulatory effects of ginseng and notoginseng on genes, functional pathways, and molecular networks. This research provides a new paradigm for exploring the mechanisms of action of traditional Chinese medicines.
JOURNAL OF PHARMACEUTICAL ANALYSIS
(2023)
Article
Chemistry, Applied
Zheng-yi Qu, He-cheng Wang, Yin-ping Jin, Ya-li Li, Ying-ping Wang
Summary: This study developed a method for isolation, identification, and quantitative determination of dammarane-type triterpene saponins in Panax notoginseng fruits, resulting in the isolation of 22 saponins with good linearity in quantitative analysis. Among them, a new saponin was identified, and the method showed good reproducibility and sensitivity for the quantification of eight major saponins.
NATURAL PRODUCT RESEARCH
(2022)
Review
Plant Sciences
Yu Xu, Mengjie Zhu, Yibin Feng, Hongxi Xu
Summary: Herbal plant-microbiome interactions play a crucial role in plant growth, health, and the production of bioactive compounds. Understanding these interactions is important for improving the performance and medicinal value of herbal plants.
Article
Plant Sciences
Wei Xiang, Jianhua Chen, Fengyuan Zhang, Rongshao Huang, Liangbo Li
Summary: The study found significant autotoxicity of root exudates from Panax notoginseng, which hindered the emergence and growth of seedlings. Allelochemicals, including benzoic acid, phthalic acid, palmitic acid, and stearic acid, were identified in the root exudates and consecutively cultivated soils, and they significantly affected the root growth of P. notoginseng.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Food Science & Technology
Xue Li, Hao Liu, Hui-Rong Yang, Ying-Jie Zeng
Summary: The study investigated the structural features and hypoglycemic effects of a polysaccharide Pan from the root of Panax notoginseng. The results showed that Pan had good antioxidant activity and could slow down body weight and blood glucose content in high-fat diet-induced mice. It further suppressed the expression of certain substances in the intestines and enhanced the expression of certain substances in the liver, exhibiting hypoglycemic effects.
Article
Environmental Sciences
Tianquan Lu, Xiaoning Wang, Xianliang Cui, Jifang Li, Jin Xu, Peng Xu, Jinpeng Wan
Summary: This study investigates the effects of Fe3O4 nanoparticles on the growth and response of Panax notoginseng to cadmium and arsenic stress. The results show that Fe3O4 nanoparticles can enhance plant tolerance, reduce heavy metal content, and alter key metabolic pathways, balancing plant growth and defense.
ENVIRONMENTAL POLLUTION
(2023)
Article
Biochemistry & Molecular Biology
Heyu Yang, Yang Ni, Xinyi Zhang, Jingling Li, Haimei Chen, Chang Liu
Summary: This study reports the mitochondrial genome sequence of Panax notoginseng. The mitochondrial genome of P. notoginseng has a multipartite structure with interconversion between a master circle and numerous subgenomic circles. Phylogenetic analysis shows that the mitochondrial genome is mostly congruent with the phylogeny of Apiales species and the chloroplast genome sequences. This study provides a foundation for understanding the evolution of Panax species.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Chemistry, Medicinal
Zheng-Yi Qu, Yu Zong, Pei-He Zheng, Yin-Ping Jin, Wei Li, He-Cheng Wang, Ya-Li Li, Ying-Ping Wang
Summary: Six malonylginsenosides, including four new compounds, were isolated from the fresh fruits of Panax notoginseng. The anti-proliferative activities of these malonylginsenosides against the SH-SY5Y human neuroblastoma cell line were confirmed using the MTT assay.
Article
Biotechnology & Applied Microbiology
Jin-Shan Jia, Na Ge, Qing-Yan Wang, Li-Ting Zhao, Cui Chen, Jun-Wen Chen
Summary: A genome-wide analysis of the LEA gene family in Panax notoginseng seeds reveals the presence of 61 LEA genes, with the LEA_6 subfamily being absent and the LEA_2 subfamily expanded. The results suggest that LEA proteins play a crucial role in the response to dehydration stress in P. notoginseng seeds, but their protective effect is not efficient.
Article
Spectroscopy
Zh. E. Ozbekova, A. A. Abdyldaev, A. A. Kulmyrzaev
Summary: This study compared two drying methods for cow and yak muscles, finding that freeze drying caused less discoloration. Additionally, analysis of fluorescence spectra allowed for accurate prediction of chemical composition and color characteristics of the muscles.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2024)
Article
Spectroscopy
Leo Mandic, Ivan Ljubic, Iva Dzeba
Summary: In this study, a combined experimental and computational approach was used to investigate the photoexcitation and photodegradation mechanisms of Doxazosin (DOX). The results provide valuable insights into the primary events following the photoexcitation of DOX and its potential applications.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2024)
Article
Spectroscopy
Xiufeng Wang, Yao Jin, Wenhui Ai, Siqi Wang, Zhiqing Zhang, Ting Zhou, Fang Wang, Guodong Zhang
Summary: This study successfully synthesized near infrared fluorescence Ag2S quantum dots (QDs) and developed a dual-mode sensor for sulfide anion using the excellent oxidase-like characteristics of manganese dioxide (MnO2) nanosheets. The sensor showed a wider detection range, higher sensitivity, and shorter reaction time, making it suitable for highly selective detection of sulfide in different concentration ranges.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2024)
Article
Spectroscopy
Yafei Li, Yanming Ma, Chuantao Zheng, Di Yu, Lien Hu, Shuo Yang, Fang Song, Yadan Li, Shuanghai Liu, Zhanrui Zhang, Yu Zhang, Yiding Wang, Frank K. Tittel
Summary: To effectively monitor gas explosion and coal spontaneous combustion in coal mine, an intrinsically safe and explosion-proof dual-gas sensor system was developed for methane (CH4) and carbon monoxide (CO) measurement. The system achieved different measurement ranges using laser scanning and gas cells, and improved stability and accuracy through gas pre-treatment and temperature compensation algorithm.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2024)
Article
Spectroscopy
Pei-Rong Chen, Li-Kang Chu
Summary: In this study, the hydrates of glyoxal were investigated using infrared absorption spectrometry. The results showed that at low concentrations, glyoxal mainly exists as monomeric dihydrate. These findings provide suitable detection windows for further research on the roles of glyoxal and its hydrates in atmospheric and aerosol chemistry, as well as the relevant reaction kinetics.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2024)
Article
Spectroscopy
Nichole O'Neill, Thamires A. Lima, Fabio Furlan Ferreira, Nicolas J. Alvarez, Reinhard Schweitzer-Stenner
Summary: This study determines the main fibril axis of GHG gel-forming fibrils using various techniques and compares the results with simulated data. The analysis suggests that the hydrophobic xz-surfaces of GHG fibrils could be a good target for the adsorption of hydrophobic drugs.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2024)
Article
Spectroscopy
Mengyuan Liu, Hanchuang Zhu, Yikun Fang, Caiyun Liu, Xinke Li, Xiaohui Zhang, Lixue Ma, Kun Wang, Miaohui Yu, Wenlong Sheng, Baocun Zhu
Summary: ABHS is an efficient fluorescent probe that can accurately and sensitively detect Al3+ with strong resistance to interference. It also demonstrates excellent detection and imaging capabilities in complex real samples.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2024)
Article
Spectroscopy
Ahmed S. El-Shafie, Evana Rahman, Yasser Gadelhak, Rehab Mahmoud, Marwa El-Azary
Summary: The recycling of waste mandarin peels into biochar (MRBC) has been used as a high performance and cost-effective adsorbent for treating polluted wastewater effluents. Batch adsorption studies were conducted to analyze the adsorption competency of MRBC on two dyes, methylene blue (MB) and basic fuchsin (BF), either in individual solutions or binary combinations. The results showed that MRBC was capable of effectively removing a high percentage of both dyes. However, the adsorption capacity decreased when the dyes were combined. The cost estimation of MRBC production and wastewater treatment indicated that both were relatively low.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2024)
Article
Spectroscopy
Anindita Bhatta, Jahnabi Upadhyaya, Dipak Chamlagai, Lincoln Dkhar, Pynskhemborlang T. Phanrang, Mohan Rao Kollipara, Sivaprasad Mitra
Summary: Derivatives of thiazole-pyrazole fused benzo-coumarin compounds were synthesized and their photophysical properties were investigated. The synthesized coumarin compounds showed potential as therapeutic agents for Alzheimer's disease, inhibiting acetylcholinesterase activity. The presence of human serum albumin was found to affect the inhibition activity.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2024)
Article
Spectroscopy
Yongwei Duan, Quancheng Liu, Yiju Zhu, Qi Zhang, Xiaohui Duan, Hu Deng, Liping Shang
Summary: In this study, the phase transition of CL-20 was observed using terahertz spectroscopy, and quantum chemical calculations were employed to analyze the vibrations. The results indicated changes in the vibrations of CL-20 before and after phase transition.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2024)
Article
Spectroscopy
Yuefeng Gao, Sai Xu, Baojiu Chen
Summary: This study synthesized Cs2NaInCl6 nanocrystals co-doped with Sb3+ and Tb3+ ions as probes for copper ions detection in lubricating oil. The introduction of Sb3+ effectively reduced the band gap of the host material and enabled energy transfer pathway for Tb3+ emission. The doped Tb3+ ions resulted in the suppression of emission due to electron transfer. The Cs2NaInCl6: 2.5 %Sb3+, 40 %Tb3+ NCs exhibited superior sensitivity and selectivity for copper ions detection.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2024)
Article
Spectroscopy
Yu Liu, Yue Zhang, Changyao Liu, Ce Wang, Baocai Xu, Li Zhao
Summary: A highly sensitive detection platform for heparin was developed using a cationic fluorescent dye (cresyl violet acetate) as a fluorescence probe. The platform demonstrated high selectivity towards heparin and achieved detection in both HEPES solution and serum.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2024)
Article
Spectroscopy
Lopamudra Roy, Nivedita Pan, Susmita Mondal, Ria Ghosh, Md. Nur Hasan, Neha Bhattacharyya, Soumendra Singh, Kallol Bhattacharyya, Arpita Chattopadhyay, Samir Kumar Pal
Summary: The study focuses on the interaction between reactive oxygen species (ROS) and a spectroscopic probe called Rose Bengal (RB), encapsulated in nanoscopic sodium dodecyl sulphate (SDS) micelles and entrapped in microscopic nylon 66 solid matrix. The research demonstrates efficient interaction between ROS and RB-SDS, and investigates the mechanism of hydroxyl radicals generation. Based on these findings, a prototype device utilizing RB embedded in a nylon thin film for quantification of ROS in extracellular fluids and food materials was developed.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2024)
Article
Spectroscopy
Xiaosong Sun, Qihao Cui, Wenyue Dong, Qian Duan, Teng Fei
Summary: Conjugated porous polymers (CPPs) are promising sensing materials and their application in aqueous media is limited. In this study, we synthesized CPPs with porous structure and prepared nanoparticles, enabling efficient photoluminescence sensing of nitroaromatic explosives in aqueous phase.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2024)
Article
Spectroscopy
Maria Cristina Caggiani, Germana Barone, Paolo Mazzoleni
Summary: Raman spectroscopy is commonly used for studying glassy materials in cultural heritage, but it is more difficult to interpret the spectra and apply the technique with portable instruments. In contrast, diffuse reflectance infrared Fourier transform spectroscopy (DRIFTS) can be used in archaeometric investigations as it is portable and non-invasive. However, there is limited application of this technique to historical glasses. This exploratory work demonstrates the potential of DRIFTS, in combination with portable X-ray Fluorescence (pXRF) and EDS microanalyses, for studying the composition and alteration of glass samples in cultural heritage.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2024)