Article
Chemistry, Multidisciplinary
Gyusik Hong, Jin Kim, Hyeongkwon Oh, Seokhwan Yun, Chul Min Kim, Yun-Mi Jeong, Won-Soo Yun, Jin-Hyung Shim, Ilho Jang, C-Yoon Kim, Songwan Jin
Summary: The study introduces a method for generating hepatic-lobule-like microtissue spheroids using a bioprinting system, which allows for the production of multiple cell-laden spheroids with high cell viability and maintained structural integrity. These structured spheroids demonstrate liver functions and exhibit stable engraftment in vivo, providing a valuable 3D model for studying liver-related applications.
ADVANCED MATERIALS
(2021)
Article
Chemistry, Multidisciplinary
Zeyu Luo, Liming Lian, Thiago Stocco, Jie Guo, Xuan Mei, Ling Cai, Syed Muntazir Andrabi, Yajuan Su, Guosheng Tang, Hossein Ravanbakhsh, Wanlu Li, Mian Wang, Xiao Kuang, Carlos Ezio Garciamendez-Mijares, Di Wang, Zongliang Wang, Junlong Liao, Maobin Xie, Jingwei Xie, Heemin Kang, Anderson Oliveira Lobo, Zongke Zhou, Yu Shrike Zhang
Summary: A cryo(bio)printing-based method is developed to fabricate shelf-ready, storable modules for scalable tissue assembly. The method involves assembling cryo(bio)printed modular scaffolds in their frozen state and subsequently bonding them together into an integral piece during the melting and photocrosslinking processes. This method overcomes the height limitation of cryo(bio)printing and allows the generation of tissue constructs of same or dissimilar materials for more precise treatment.
ADVANCED FUNCTIONAL MATERIALS
(2023)
Article
Nanoscience & Nanotechnology
Xiuli Wang, Chengchen Guo, Lina Guo, Mingqi Wang, Ming Liu, Yizhe Song, Hui Jiao, Xiaoqing Wei, Zinan Zhao, David L. Kaplan
Summary: A versatile strategy was developed to fabricate biomimetic hepatic lobules in vitro, utilizing silk protein scaffolds with radially aligned lamellar sheets and interconnected channels. Coculturing human hepatocytes and primary endothelial cells on these scaffolds resulted in enhanced hepatocyte functions and physiological cell organization, replicating hepatic-like parenchymal-mesenchymal growth patterns. This biomimetic technology holds potential for applications in bioartificial liver engineering and drug metabolism investigation.
ACS APPLIED MATERIALS & INTERFACES
(2022)
Review
Chemistry, Multidisciplinary
Maurice N. Collins, Guang Ren, Kieran Young, S. Pina, Rui L. Reis, J. Miguel Oliveira
Summary: Bone tissue engineering aims to create biofunctional tissue for treating diseases, with scaffold fabrication techniques and biomaterial selection being crucial. Vascularization and immunomodulation are major challenges in this field.
ADVANCED FUNCTIONAL MATERIALS
(2021)
Review
Engineering, Biomedical
Nikolas Di Caprio, Jason A. Burdick
Summary: Cellular spheroids are being explored as building blocks for tissue engineering and as tissue models. Biomaterials are being used to guide spheroid formation and function, and also facilitate the fabrication of tissue constructs. This review provides an overview of recent studies on the role of engineered biomaterials in supporting spheroids in biomedical applications.
ACTA BIOMATERIALIA
(2023)
Article
Engineering, Biomedical
Yongcong Fang, Mengke Ji, Yi Yang, Yihan Guo, Ruobin Sun, Ting Zhang, Wei Sun, Zhuo Xiong
Summary: Three-dimensional bioprinting has been hindered by a lack of suitable bioinks for printing functional tissues. We developed a granular cell aggregate-based biphasic (GCAB) bioink that exhibited desired properties for extrusion bioprinting and modeling the mechanical characteristics of soft tissues. By preorganizing GCAB bioink and printing it with an endothelial cell-laden gelatin bioink, we successfully created functional hepatic tissues with high cell density and a perfusable vascular network. This GCAB bioink design has potential for creating functional tissues for therapeutic applications.
Article
Biotechnology & Applied Microbiology
Hae Sang Park, Ji Seung Lee, Chang-Beom Kim, Kwang-Ho Lee, In-Sun Hong, Harry Jung, Hanna Lee, Young Jin Lee, Olatunji Ajiteru, Md Tipu Sultan, Ok Joo Lee, Soon Hee Kim, Chan Hum Park
Summary: In this study, we successfully created a three-dimensional bioprinted artificial larynx using rabbit's chondrocyte-laden gelatin methacryloyl (GelMA)/glycidyl-methacrylated hyaluronic acid (GMHA) hybrid bioink. We incorporated a polycaprolactone (PCL) outer framework with pores to provide structural strength and a suitable microenvironment for the printed cells. Our novel fluidics supply (FS) system improved cell viability during the printing process, leading to the generation of a large-scale cell-laden artificial laryngeal framework. Animal studies confirmed the functionality of the transplanted 3D bio-larynx, offering potential for clinical applications in laryngectomy patients.
BIOENGINEERING & TRANSLATIONAL MEDICINE
(2023)
Review
Engineering, Biomedical
Yongcong Fang, Wei Sun, Ting Zhang, Zhuo Xiong
Summary: This review highlights the recent advances in bioengineering approaches for fabricating functional engineered cardiac pumps, focusing on methods for creating ECPs and the potential of 3D bioprinting techniques. It emphasizes the importance of high cell density, engineering vasculatures, and strategies for functional maturation of cardiac tissues.
Article
Engineering, Biomedical
Mozhgan Keshavarz, Mohammadjavad Jahanshahi, Masoud Hasany, Firoz Babu Kadumudi, Mehdi Mehrali, Mohammad-Ali Shahbazi, Parvin Alizadeh, Gorka Orive, Alireza Dolatshahi-Pirouz
Summary: Significant progress has been made in tissue engineering, but challenges remain. Bioprinting is an innovative technology that can help overcome these challenges. The new trend is 4D bioprinting, which allows printed constructs to change shape and function over time. Alginate, a key material, plays a crucial role in advancing bioprinting.
MATERIALS TODAY BIO
(2023)
Article
Biotechnology & Applied Microbiology
Ivonne Gonzalez-Gamboa, Edith Velazquez-Lam, Matias Jose Lobo-Zegers, Ada Itzel Frias-Sanchez, Jorge Alfonso Tavares-Negrete, Andrea Monroy-Borrego, Jorge Luis Menchaca-Arrendondo, Laura Williams, Pablo Lunello, Fernando Ponz, Mario Moises Alvarez, Grissel Trujillo-de Santiago
Summary: TuMV, a plant viral nanoparticle, shows promise as an additive for GelMA hydrogels in engineering mammalian tissues. Its nanomesh structure can serve as a nanoscaffold for cells. Covalently conjugating TuMV particles with EGF increases cell confluency on the functionalized scaffolds.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Review
Pharmacology & Pharmacy
Annika C. Dell, Grayson Wagner, Jason Own, John P. Geibel
Summary: 3D bioprinting plays a crucial role in tissue engineering in medicine by providing precise and highly customizable methods for creating biological tissues. The selection of appropriate cell ink formulations is vital for process optimization and customization to suit target tissues. Hydrogel-based cell ink formulations show promise for future development in bioprinting technology.
Article
Engineering, Biomedical
Alejandro Madrid-Sanchez, Fabian Duerr, Yunfeng Nie, Hugo Thienpont, Heidi Ottevaere
Summary: The characteristics of high porosity, microscale features, and pores interconnectivity make scaffolds suitable for human tissue substitutes. However, these characteristics often limit the scalability of different fabrication approaches, especially in bioprinting techniques. In this study, an alternative vat photopolymerization technique called light sheet stereolithography (LS-SLA) is proposed to fabricate centimeter-scale scaffolds with high resolution. The LS-SLA technology allows the modification of voxel profiles using laser beam shaping and enables the production of complex three-dimensional scaffolds.
INTERNATIONAL JOURNAL OF BIOPRINTING
(2023)
Article
Engineering, Biomedical
Shannon T. T. McLoughlin, Abigail R. R. McKenna, John P. P. Fisher
Summary: Traditional methods are unable to effectively fabricate thin membranous tissues, while 4D bioprinting technology can generate cell-laden thin membranes. By using anionic gelatin methacrylate (GelMA) and cationic poly-l-lysine (PLL), shrinking of the membrane and cell viability can be achieved. This finding is of significant importance in addressing critical barriers in tissue engineering of thin membranes.
ADVANCED HEALTHCARE MATERIALS
(2023)
Review
Engineering, Biomedical
Chen He, Jiasheng Yan, Yusheng Fu, Jiuchuan Guo, Yuxing Shi, Jinhong Guo
Summary: Organoids are three-dimensional cell structures that can be cultured in vitro and mimic real organs in both structure and function. The development of bioprinting technology has overcome certain limitations in organoid manufacturing, enabling their wider application in drug screening, regenerative medicine, and other fields.
INTERNATIONAL JOURNAL OF BIOPRINTING
(2023)
Article
Chemistry, Analytical
Bo Yang, Tianqi Liu, Ge Gao, Xianglin Zhang, Bin Wu
Summary: This study explores the GelMA/Agarose suspension printing system and defines a suitable printing window. The resulting scaffolds have good water absorption and elasticity but exhibit significant deformation during printing.
Article
Biochemistry & Molecular Biology
Tae-Min Park, Donggu Kang, Ilho Jang, Won-Soo Yun, Jin-Hyung Shim, Young Hun Jeong, Jong-Young Kwak, Sik Yoon, Songwan Jin
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2017)
Article
Multidisciplinary Sciences
Geunseon Ahn, Kyung-Hyun Min, Changhwan Kim, Jeong-Seok Lee, Donggu Kang, Joo-Yun Won, Dong-Woo Cho, Jun-Young Kim, Songwan Jin, Won-Soo Yun, Jin-Hyung Shim
SCIENTIFIC REPORTS
(2017)
Article
Chemistry, Multidisciplinary
Binh Duong Le, Donggu Kang, Seokhwan Yun, Young Hun Jeong, Jong-Young Kwak, Sik Yoon, Songwan Jin
Article
Engineering, Environmental
HoJun Jeon, Minji Lee, Seokhwan Yun, Donggu Kang, Keun-ho Park, Seongjun Choi, Eunjeong Choi, Songwan Jin, Jin-Hyung Shim, Won-Soo Yun, Beom-Jin Yoon, Jongbong Park
CHEMICAL ENGINEERING JOURNAL
(2019)
Article
Chemistry, Multidisciplinary
Hojun Jeon, Seokhwan Yun, Eunjeong Choi, Donggu Kang, Keun-ho Park, Dongyun Kim, Songwan Jin, Jin-Hyung Shim, Won-Soo Yun, Jongbong Park
JOURNAL OF INDUSTRIAL AND ENGINEERING CHEMISTRY
(2019)
Article
Materials Science, Biomaterials
Eunjeong Choi, Dongyun Kim, Donggu Kang, Gi Hoon Yang, Bongsu Jung, MyungGu Yeo, Min-Jeong Park, SangHyun An, KyoungHo Lee, Jun Sik Kim, Jong Chul Kim, Woonhyeok Jeong, Hye Hyun Yoo, Hojun Jeon
Summary: In this study, a two-stage temperature control system was proposed to overcome the low processability of GelMA in 3D bioprinting due to its temperature-dependent viscosity. The composite scaffold with higher silica content (10wt%) exhibited enhanced physical and biological performances compared to controls, suggesting its potential for hard tissue regeneration.
REGENERATIVE BIOMATERIALS
(2021)
Article
Materials Science, Multidisciplinary
Gi Hoon Yang, MyungGu Yeo, Eunjeong Choi, Donggu Kang, Minseong Kim, Yunjoo Nam, So-Jung Gwak, Hye Hyun Yoo, Min-Jeong Park, Bongsu Jung, Woonhyeok Jeong, Hojun Jeon
Summary: The study found that different sizes of silica particles have varying effects on the physical and biological properties of polycaprolactone scaffolds. Particles with a size of 800 nm can improve the physical properties of the scaffold, while particles with a size of 100 nm can significantly enhance the biological properties of the scaffold.
MATERIALS & DESIGN
(2021)
Review
Engineering, Biomedical
Gi Hoon Yang, Yoon Bum Lee, Donggu Kang, Eunjeong Choi, Yoonju Nam, Kyoung Ho Lee, Hi-Jin You, Hyo Jin Kang, Sang Hyun An, Hojun Jeon
Summary: Exosomes are nano-sized vesicles carrying diverse biomolecules, serving as potential biomarkers in skin therapy. They play a crucial role in communication and regeneration of skin cells, but face challenges in engineering and commercialization. Further research is needed to overcome limitations and explore future perspectives in exosome technology for skin treatments.
BIOMATERIALS RESEARCH
(2021)
Article
Engineering, Biomedical
Donggu Kang, Geunseon Ahn, Donghwan Kim, Hyun-Wook Kang, Seokhwan Yun, Won-Soo Yun, Jin-Hyung Shim, Songwan Jin
Article
Engineering, Biomedical
Donggu Kang, Jeong Hwa Kim, Young Hun Jeong, Jong-Young Kwak, Sik Yoon, Songwan Jin