期刊
SENSORS
卷 19, 期 24, 页码 -出版社
MDPI
DOI: 10.3390/s19245560
关键词
interdigitated wave-shaped microelectrode array; self-assembled monolayer; C-reactive protein; immunosensor; atomic force microscopy; impedance spectroscopy
资金
- National Research Foundation of Korea
- NRF [2017R1D1A1A09000712, 2018R1C1B6009385, 2018M3A9F1023690, 2019R1A2C1088680]
- National Research Foundation of Korea [2017R1D1A1A09000712, 2018R1C1B6009385, 2019R1A2C1088680] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
An electrochemical capacitance immunosensor based on an interdigitated wave-shaped micro electrode array (IDW mu E) for direct and label-free detection of C-reactive protein (CRP) was reported. A self-assembled monolayer (SAM) of dithiobis (succinimidyl propionate) (DTSP) was used to modify the electrode array for antibody immobilization. The SAM functionalized electrode array was characterized morphologically by atomic force microscopy (AFM) and energy dispersive X-ray spectroscopy (EDX). The nature of gold-sulfur interactions on SAM-treated electrode array was probed by X-ray photoelectron spectroscopy (XPS). The covalent linking of anti-CRP-antibodies onto the SAM modified electrode array was characterized morphologically through AFM, and electrochemically through cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). The application of phosphate-buffered saline (PBS) and human serum(HS) samples containing different concentrations of CRP in the electrode array caused changes in the electrode interfacial capacitance upon CRP binding. CRP concentrations in PBS and HS were determined quantitatively by measuring the change in capacitance (Delta C) through EIS. The electrode immobilized with anti-CRP-antibodies showed an increase in Delta C with the addition of CRP concentrations over a range of 0.01-10,000 ng mL(-1). The electrode showed detection limits of 0.025 ng mL(-1) and 0.23 ng mL(-1) (S/N = 3) in PBS and HS, respectively. The biosensor showed a good reproducibility (relative standard deviation (RSD), 1.70%), repeatability (RSD, 1.95%), and adequate selectivity in presence of interferents towards CRP detection. The sensor also exhibited a significant storage stability of 2 weeks at 4 degrees C in 1 x PBS.
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