期刊
BIOSENSORS & BIOELECTRONICS
卷 63, 期 -, 页码 248-254出版社
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2014.07.047
关键词
Biophotonics; Photonic crystal fibers; DNA detection; Peptide nucleic acids; Optofluidics
类别
资金
- Italian MIUR Ministry [PRIN20093N774P]
- AIRC (Associazione Italiana per la Ricerca sul Cancro) [IG13575]
Microstructured optical fibers containing microchannels and Bragg grating inscribed were internally functionalized with a peptide nucleic acid (PNA) probe specific for a gene tract of the genetically modified Roundup Ready soy. These fibers were used as an optofluidic device for the detection of DNA by measuring the shift in the wavelength of the reflected IR light. Enhancement of optical read-out was obtained using streptavidin coated gold-nanoparticles interacting with the genomic DNA captured in the fiber channels (0%, 0.1%, 1% and 10% RR-Soy), enabling to achieve statistically significant, label-free, and amplification-free detection of target DNA in low concentrations, low percentages, and very low sample volumes. Computer simulations of the fiber optics based on the finite element method (FEM) were consistent with the formation of a layer of organic material with an average thickness of 39 nm for the highest percentage (10% RR soy) analysed. (C) 2014 Elsevier B.V. All rights reserved.
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