期刊
BIOSENSORS & BIOELECTRONICS
卷 68, 期 -, 页码 404-412出版社
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2015.01.023
关键词
Biosensor; Mixed brushes; Glucose; Liquid-crystal; 5CB; TEM
类别
资金
- National Research Foundation of Korea [NRF-2011-0020264]
- National Research Foundation of Korea [21A20131800001] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
4-Cyano-4'-pentylbiphenyl (5CB) in a transmission electron microscopy (TEM) grid was developed for glucose detection by coating with a monolayer of mixed polymer brushes using poly(acrylicacid-b-4-cynobiphenyl-4'-oxyundecylacrylate) (PAA-b-LCP) and quaternized poly(4-vinylpyridine-b-4-cynobiphenyl-4'-oxyundecylacrylate) (QP4VP-b-LCP) (LCP stands for liquid crystal polymer) at the 5CB/aqueous interface. The resultant 5CB in TEM grid was functionalized with the PAA and QP4VP brushes, which were strongly anchored by the LCP block. The PAA brush rendered the 5CB/aqueous interface pH-responsive and the QP4VP brush immobilized glucose oxidase (GOx) through electrostatic interactions without the aid of coupling agents. The glucose was detected through a homeotropic-to-planar orientational transition of the 5CB observed through a polarized optical microscope (POM) under crossed polarizers. The optimum immobilization with a 0.78 mu M GOx solution on the dual-brush-coated TEM grid enabled glucose detection at concentrations higher than 0.5 mM with response times shorter than 180 s. This TEM grid glucose sensor provided a linear response of birefringence of the 5CB to glucose concentrations ranging from 0.5 to 11 mM with a Michaelis-Menten constant (K-m) of 1.67 mM. This new and sensitive glucose biosensor has the advantages of low production cost, simple enzyme immobilization, high enzyme sensitivity and stability, and easy detection with POM, and may be useful for prescreening the glucose level in the human body. (C) 2015 Elsevier B.V. All rights reserved.
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