期刊
PROCESS BIOCHEMISTRY
卷 93, 期 -, 页码 55-62出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.procbio.2020.02.028
关键词
Myriophyllum aquaticum; Constructed wetlands; Ammonium nitrogen concentrations; Microbial community structure; Denitrifiers
资金
- Knowledge Innovation Program of Shenzhen [JSGG20171012143231779]
- National Science Fund Projects [31700429]
- Key Research and Development Plan of Ningxia Hui Autonomous Region [2019BFG02032]
Constructed wetlands (CWs) have received increasing attentions for their N removal performances, especially regarding NH4+-N. Different influent NH4+-N concentration may influence N removal efficiency in practice, while the effects of different NH4+-N concentrations on microorganisms removing N in CWs are poorly understood. In this study, surface flow CWs planted with Myriophyllum (M). aquaticum were established to investigate the influences of different NH4+-N concentrations on the composition, structure, and interactions of microbial community. Our findings suggested 105 mg/L NH4+-N CWs achieved highest N removal rate, removing 89.30 % NH4+-N and 92.34 % TN from the influent. The results of real-time quantitative polymerase chain reactions (qPCR) indicated abundances of nitrifying genes (nxrA) and denitrifying genes (narG, nirS, nirK, and nosZ) were increased by increasing NH4+-N concentrations, and the strongest effects were observed in narG (8-fold) and nosZ genes (11-fold). Different NH4+-N concentrations was proved to alter composition and structure of microbial communities via high-throughput sequencing, e.g. denitrifiers including Brevendomonas.sp, Dokdonella.sp and Rhodococcus.sp were enriched obviously with increasing NH4+-N concentrations. In addition, network showed interactions among microbial populations and positive interactions were dramatically shifted and enhanced by increasing NH4+-N concentrations.
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