期刊
BIOSENSORS & BIOELECTRONICS
卷 69, 期 -, 页码 95-99出版社
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2015.02.022
关键词
Secretoneurin; Reduced graphene oxide-tetraethylene pentamine; Lead ion; Au@graphene nanosheeets
类别
资金
- National Natural Science Foundation of China [81370403, 21205146]
- Funds for outstanding young scholars in Chongqing Medical University [CYYQ201309]
In this work, a novel electrochemical immunosensor for the detection of secretoneurin (SN), which uses metal ion functionalised reduced graphene oxide-tetraethylene pentamine (rGO-TEPA) as a label, is reported for the first time. rGO-TEPA contains a large number of amino groups, which makes it an ideal templet for the loading of metal ions. rGO-TEPA-Pb2+ was employed to immobilise secondary secretoneurin (SN) antibody (AIDA, and the resulting nanocomposite (Ab(2)-rGO-TEPA-Pb2+) was used as a trace tag for signal amplification. A modified electrode consisting of functionalised graphene nanosheets (Au@GS) was used as a substrate to immobilise the antibodies. Under the optimal conditions, the immunoassay exhibited high sensitivity, acceptable stability and reproducibility with a wide linear range from 0.001 to 100 ng mL(-1) (R=0.996), and an ultra-low detection limit of 0.33 pg mL(-1) (SIN=3). Furthermore, the immunosensor could be employed to detect SN in clinical serum samples. The proposed sensing strategy enriches the electrochemical immunoassay and exhibits potential for the point-of-care diagnostic application of the clinical screening of biomarkers. (C) 2015 Elsevier B.V. All rights reserved.
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