Article
Physiology
Iris Louise Eleonora van de Pol, Adam Hermaniuk, Wilhelmus Cornelis Egbertus Petrus Verberk
Summary: Research shows that cell size affects gene expression, growth, and swimming performance of ectotherms at different temperatures, with these effects being temperature and ploidy-dependent. Triploids with larger cells outperformed diploids in cooler conditions, while diploids performed better in warmer conditions.
FRONTIERS IN PHYSIOLOGY
(2021)
Article
Environmental Sciences
Ahmed Nasri, Ali Mezni, Pierre-Andre Lafon, Aymen Wahbi, Nicolas Cubedo, Philippe Clair, Abdel Halim Harrath, Hamouda Beyrem, Mireille Rossel, Veronique Perrier
Summary: Research shows that ethinylestradiol has negative effects on the nervous system development of zebrafish larvae, including disruption of nerve regeneration and swimming behavior, as well as alteration in gene expression levels.
SCIENCE OF THE TOTAL ENVIRONMENT
(2021)
Article
Biology
Harsh Vashistha, Maryam Kohram, Hanna Salman
Summary: The heterogeneity of cells within an isogenic population is influenced by stochasticity in intracellular biochemical processes and resource distribution during divisions, but is limited by the inheritance of cellular components between generations. A new experimental method has been introduced to measure proliferation of heterogeneity in bacterial cell characteristics by tracking the differentiation between sister cells over time. Inherited dynamics of cellular properties and the long-term memory of cells to maintain these properties reveal mechanisms of non-genetic inheritance in bacteria and how cells control properties and heterogeneity within isogenic populations.
Article
Multidisciplinary Sciences
Sanjeeva Metikala, Satish Casie Chetty, Saulius Sumanas
Summary: In this study, single-cell RNA-seq was used to analyze over 20,000 cells disaggregated from the trunk region of zebrafish embryos at the 30 hpf stage, identifying and annotating transcriptional signatures of 27 different cell types and subtypes. This dataset will serve as a valuable resource for researchers in developmental and cellular biology, aiding in the understanding of molecular mechanisms that regulate cell lineage choices during development.
Article
Environmental Sciences
Jiefeng Liang, Xiaoxi Yang, Tongtong Xiang, Xuanyue Chen, Zhihua Ren, Xiaoyun Wang, Jiahui Su, Yuzhu Zhang, Qian S. Liu, Guangbo Qu, Qunfang Zhou, Guibin Jiang
Summary: This study tested the effects of four commonly-used parabens on the neuroendocrine system of zebrafish larvae and found that they significantly reduced swimming distance and mean velocity. Paraben exposure also increased ACTH levels and decreased cortisol levels in the larvae, while down-regulating the expressions of target genes in the HPI axis. It was further revealed that parabens could bind with the glucocorticoid receptor and trigger its transactivation. These findings contribute to the understanding of the potential deleterious effects of parabens on the neuroendocrine system.
SCIENCE OF THE TOTAL ENVIRONMENT
(2023)
Article
Biochemical Research Methods
Ben Korin, Jun-Jae Chung, Shimrit Avraham, Andrey S. Shaw
Summary: This protocol describes a method for isolating high-quality mouse glomerular cells for high-throughput analysis, which involves direct perfusion of Dynabeads and isolation by magnet to efficiently purify and obtain single glomerular cells with high yield and viability. The balanced representation of different cell types in the glomerulus and high cell viability confirm the efficiency of this method, providing a valuable technique for future studies on glomerular cells in health, injury, and disease.
Article
Fisheries
Andrea C. Boaglio, Andrea C. Mendia Broda, Lautaro H. Munoz, Maria Belen Mascambroni, Pablo A. Lopez, Esteban Moron-Aleain, Silvia E. Arranz, Fabricio A. Vigliano
Summary: Diploid Rhamdia quelen showed greater susceptibility to short-term starvation compared to triploid fish. Differential physiological responses to alimentary changes between diploid and triploid fish may be relevant for optimizing culture conditions.
Article
Multidisciplinary Sciences
Chuyi Chen, Yuyang Gu, Julien Philippe, Peiran Zhang, Hunter Bachman, Jinxin Zhang, John Mai, Joseph Rufo, John F. Rawls, Erica E. Davis, Nicholas Katsanis, Tony Jun Huang
Summary: A novel acoustofluidic rotational tweezing platform has been developed for contactless, high-speed, 3D multispectral imaging and digital reconstruction of zebrafish larvae, allowing quantitative phenotypic analysis. This technology has the potential to be a valuable asset in various fields, especially in biomedical research, biochemistry screening, and preclinical drug development.
NATURE COMMUNICATIONS
(2021)
Article
Environmental Sciences
Yuyang Lei, Fangfang Li, Monika Mortimer, Zhi Li, Bi-Xia Peng, Minjie Li, Liang-Hong Guo, Guoqiang Zhuang
Summary: Antibiotics as emerging environmental contaminants have adverse effects on lipid metabolism, but the underlying toxicological mechanisms are still unclear.
SCIENCE OF THE TOTAL ENVIRONMENT
(2023)
Article
Environmental Sciences
Jun Zhu, Yi Zhang, Yawen Xu, Li Wang, Qian Wu, Zhan Zhang, Lei Li
Summary: It has been found in this study that microplastics (MPs) can absorb methylmercury (MeHg) and enhance its accumulation in zebrafish, resulting in reduced locomotor activity and downregulation of neurotransmitter-related genes. This disrupts the health of zebrafish by excessive oxidative stress. This study provides scientific evidence for improving health risk assessment of environmental pollutants.
MARINE ENVIRONMENTAL RESEARCH
(2022)
Article
Marine & Freshwater Biology
Qi Meng, Karen Yeung, King Ming Chan
Summary: Studies have shown that Octocrylene (OC) has toxic effects on zebrafish and zebrafish liver cells, inducing gene expression and affecting hormone synthesis and antioxidant pathways. The findings support banning the use of OC in cosmetics.
AQUATIC TOXICOLOGY
(2021)
Review
Pharmacology & Pharmacy
Jeffrey J. Widrick, Matthias R. Lambert, Louis M. Kunkel, Alan H. Beggs
Summary: This review article summarizes the application of zebrafish larvae in large-scale drug discovery, focusing on the experimental methods and interpretation of swimming behavior. Attention to animal husbandry, experimental design, data acquisition, and interpretation of results can improve the accuracy and stability of screening outcomes.
EXPERT OPINION ON DRUG DISCOVERY
(2023)
Article
Toxicology
Benedikt Bauer, Daniel Liedtke, Sebastian Jarzina, Emilia Stammler, Katrin Kreisel, Viola Lalomia, Markus Diefenbacher, Eva Klopocki, Angela Mally
Summary: Zebrafish larvae are considered a promising alternative whole-animal model for assessing systemic toxicity. Exposure of zebrafish larvae to various nephrotoxins resulted in kidney injury and impaired function. Gene expression analysis showed the most prominent changes in larvae treated with gentamicin compared to other nephrotoxins.
TOXICOLOGY LETTERS
(2021)
Article
Environmental Sciences
Peng Xiao, Wenhua Li, Jinfang Lu, He Zhang
Summary: In this study, the adverse effects of amisulbrom and isoflucypram on zebrafish embryogenesis were investigated. Transcriptome sequencing analysis revealed differentially expressed genes (DEGs) after exposure to the fungicides. Enrichment analysis identified important signaling pathways and metabolic pathways. These findings provide novel insights into the toxicological mechanisms underlying fish's responses to fungicides.
Article
Neurosciences
Cagla Ozsoy, Adriana L. Hotz, Nicolas N. Rieser, Zhenyue Chen, Xose Luis Dean-Ben, Stephan C. F. Neuhauss, Daniel Razansky
Summary: This study reports on a real-time volumetric optoacoustic imaging technique that captures abnormal swimming activity and calcium transients in freely behaving zebrafish, providing insights into the functional interconnections between zebrafish behavior and neuronal activity.
FRONTIERS IN MOLECULAR NEUROSCIENCE
(2022)
Article
Biology
Wilco C. E. P. Verberk, David Atkinson, K. Natan Hoefnagel, Andrew G. Hirst, Curtis R. Horne, Henk Siepel
Summary: Body size plays a central role in ecology, with temperature-induced variations in body size being of interest. The temperature-size rule states that warmer conditions tend to result in smaller body sizes at maturity. Oxygen limitation may play a significant role in this response.
BIOLOGICAL REVIEWS
(2021)
Article
Biology
Adam Hermaniuk, Iris L. E. van de Pol, Wilco C. E. P. Verberk
Summary: The study found that temperature affects the costs and benefits of cell size, with animals composed of smaller cells being less vulnerable to oxygen limitation in warm water and animals composed of larger cells having metabolic advantages in cold water.
JOURNAL OF EXPERIMENTAL BIOLOGY
(2021)
Review
Biology
Michael Collins, Manuela Truebano, Wilco C. E. P. Verberk, John Spicer
Summary: Aquatic ectotherms may experience changes in hypoxic performance due to climate warming and thermal acclimation. Most studies focus on testing hypoxic responses at temperatures matching organisms' acclimation temperatures, limiting the interpretation of thermal acclimation effects. Some studies suggest that warm acclimation may improve hypoxic performance in aquatic ectotherms.
JOURNAL OF EXPERIMENTAL BIOLOGY
(2021)
Article
Ecology
Joost J. Vogels, W. C. E. P. Verberk, J. T. Kuper, M. J. Weijters, R. Bobbink, H. Siepel
Summary: Experimental studies conducted in European dry heathlands have shown that liming and P addition have different effects on invertebrate performance. P addition stimulated daily reproduction of crickets, while liming significantly reduced reproduction quantity.
FRONTIERS IN ECOLOGY AND EVOLUTION
(2021)
Article
Environmental Sciences
Lizanne Janssens, Wilco Verberk, Robby Stoks
Summary: Trait-based approaches show promise in predicting species and populations' sensitivity to pesticides. Metabolic rate and surface area are key traits that may influence sensitivity. Our study found that fast-paced populations of Ischnura elegans had higher pesticide tolerance due to faster elimination, contrasting with slow-paced populations. Gill loss had opposite effects on pesticide sensitivity, likely due to the balance between pesticide uptake and oxygen intake. These results stress the importance of considering trait interactions in predicting pesticide sensitivity.
ENVIRONMENTAL POLLUTION
(2021)
Article
Entomology
Andrzej Anto, Matty P. Berg, Wilco C. E. P. Verberk
Summary: The study suggests that terrestrial isopods have evolved a trade-off between water retention and gas exchange, influenced by species differences and body size effects. By comparing Porcellio scaber and Oniscus asellus, it was found that species identity and body size significantly affect tolerance to hypoxia and low humidity.
JOURNAL OF INSECT PHYSIOLOGY
(2021)
Article
Biology
Dylan J. M. Bergen, Qiao Tong, Ankit Shukla, Elis Newham, Jan Zethof, Mischa Lundberg, Rebecca Ryan, Scott E. Youlten, Monika Frysz, Peter I. Croucher, Gert Flik, Rebecca J. Richardson, John P. Kemp, Chrissy L. Hammond, Juriaan R. Metz
Summary: Dermal scales possess a strong osteogenic expression profile similar to bone, enriched in genes favoring collagen matrix growth. Despite differences in developmental processes between scale and endoskeleton, zebrafish scales express a subset of evolutionarily conserved genes relevant to human skeletal diseases.
Article
Biology
Susana Pallares, Wilco C. E. P. Verberk, David T. Bilton
Summary: The study assesses the impact of thermal acclimation on locomotion activity and metabolism in a water beetle endemic to shallow saline streams in SE Spain. High temperature acclimated beetles show higher maximum locomotor activity and reduced thermal performance curve breadth, as well as increased metabolic rates achieved primarily through aerial oxygen uptake. Temperature acclimation may play a crucial role in the response of aquatic insects to climate change, with implications for future vulnerability to warming temperatures.
JOURNAL OF THERMAL BIOLOGY
(2021)
Article
Physiology
Iris Louise Eleonora van de Pol, Adam Hermaniuk, Wilhelmus Cornelis Egbertus Petrus Verberk
Summary: Research shows that cell size affects gene expression, growth, and swimming performance of ectotherms at different temperatures, with these effects being temperature and ploidy-dependent. Triploids with larger cells outperformed diploids in cooler conditions, while diploids performed better in warmer conditions.
FRONTIERS IN PHYSIOLOGY
(2021)
Article
Biodiversity Conservation
Wilco C. E. P. Verberk, Jeroen F. Sandker, Iris L. E. van de Pol, Mauricio A. Urbina, Rod W. Wilson, David J. McKenzie, Felix P. Leiva
Summary: This study assesses the variation in hypoxia tolerance observed in fishes within a phylogenetic context. It found that hypoxia tolerance has a clear phylogenetic signal and is affected by factors such as temperature, body mass, cell size, salinity, and metabolic rate. Marine fishes are more susceptible to hypoxia than freshwater fishes, and fishes with higher oxygen requirements are also more susceptible to hypoxia. Additionally, hypoxia and warming may act synergistically, leading to lower hypoxia tolerance in warmer waters.
GLOBAL CHANGE BIOLOGY
(2022)
Article
Multidisciplinary Sciences
Curtis Deutsch, Justin L. Penn, Wilco C. E. P. Verberk, Keisuke Inomura, Martin-Georg Endress, Jonathan L. Payne
Summary: Rising temperatures are associated with reduced body size in many marine species, and the mechanism behind this phenomenon is still debated. A predictive model for body size responses to temperature and oxygen changes is derived, taking into account the thermal and geometric constraints on organismal oxygen supply and demand. The study shows that oxygen limitation is the mechanism underlying the temperature-size rule, providing a physiological basis for projecting ectotherm body size responses to climate change.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Review
Ecology
Jeffrey A. Harvey, Kevin Tougeron, Rieta Gols, Robin Heinen, Mariana Abarca, Paul K. Abram, Yves Basset, Matty Berg, Carol Boggs, Jacques Brodeur, Pedro Cardoso, Jetske G. de Boer, Geert R. De Snoo, Charl Deacon, Jane E. Dell, Nicolas Desneux, Michael E. Dillon, Grant A. Duffy, Lee A. Dyer, Jacintha Ellers, Anahi Espindola, James Fordyce, Matthew L. Forister, Caroline Fukushima, Matthew J. G. Gage, Carlos Garcia-Robledo, Claire Gely, Mauro Gobbi, Caspar Hallmann, Thierry Hance, John Harte, Axel Hochkirch, Christian Hof, Ary A. Hoffmann, Joel G. Kingsolver, Greg P. A. Lamarre, William F. Laurance, Blas Lavandero, Simon R. Leather, Philipp Lehmann, Cecile Le Lann, Margarita M. Lopez-Uribe, Chun-Sen Ma, Gang Ma, Joffrey Moiroux, Lucie Monticelli, Chris Nice, Paul J. Ode, Sylvain Pincebourde, William J. Ripple, Melissah Rowe, Michael J. Samways, Arnaud Sentis, Alisha A. Shah, Nigel Stork, John S. Terblanche, Madhav P. Thakur, Matthew B. Thomas, Jason M. Tylianakis, Joan Van Baaren, Martijn Van de Pol, Wim H. Van der Putten, Hans Van Dyck, Wilco C. E. P. Verberk, David L. Wagner, Wolfgang W. Weisser, William C. Wetzel, H. Arthur Woods, Kris A. G. Wyckhuys, Steven L. Chown
Summary: Climate warming is a serious anthropogenic stress on the environment, exacerbating the harmful effects of other threats and potentially threatening species preservation and ecosystem services provided by biodiversity. Insects, as central components of many ecosystems, are highly affected by climate change, with effects on physiology, behavior, distribution, and interactions, as well as extreme events.
ECOLOGICAL MONOGRAPHS
(2023)
Article
Ecology
Felix P. Leiva, Jeroen G. J. Boerrigter, Wilco C. E. P. Verberk
Summary: The size of an animal's body is closely related to its key ecological traits, such as fecundity, mortality, and growth. Ectothermic animals mature at smaller body sizes in warmer conditions and low oxygen availability (hypoxia). It is poorly understood whether these responses are due to changes in cell size and cell number, and how these cellular responses help ectotherms cope with heat and hypoxia.
FUNCTIONAL ECOLOGY
(2023)
Article
Biodiversity Conservation
Wilco C. E. P. Verberk, K. Natan Hoefnagel, Ignacio Peralta-Maraver, Mathieu Floury, Enrico L. Rezende
Summary: Forecasting long-term consequences of global warming requires considering thermal mortality and how heat stress interacts with other environmental stressors. A flexible analytical framework combining laboratory measurements and field temperature records is described to forecast mortality risks. The framework incorporates physiological acclimation effects, temporal scale differences, and the ecological reality of temperature fluctuations and other factors. The findings indicate a significant increase in annual mortality due to rising summer temperatures, and the effects of thermal acclimation and adequate oxygenation play a crucial role. However, even with the best-case scenario, mortality of certain species is expected to approach 100% by 2100.
GLOBAL CHANGE BIOLOGY
(2023)
Article
Biology
H. Arthur Woods, Amy L. Moran, David Atkinson, Asta Audzijonyte, Michael Berenbrink, Francisco O. Borges, Karen G. Burnett, Louis E. Burnett, Christopher J. Coates, Rachel Collin, Elisa M. Costa-Paiva, Murray I. Duncan, Rasmus Ern, Elise M. J. Laetz, Lisa A. Levin, Max Lindmark, Noelle M. Lucey, Lillian R. McCormick, James J. Pierson, Rui Rosa, Michael R. Roman, Eduardo Sampaio, Patricia M. Schulte, Erik A. Sperling, Aleksandra Walczynska, Wilco C. E. P. Verberk
Summary: Oxygen bioavailability is decreasing in aquatic systems globally. This article outlines the causes and consequences of this decline and proposes approaches for future research.
BIOLOGICAL BULLETIN
(2022)