Article
Infectious Diseases
Camille Bouchand, Anais Andreo, Florence Le Gallou, Stephane Corvec, Celine Bourigault, Didier Lepelletier
Summary: The retrospective study conducted from 2005 to 2019 showed a significant increase in the incidence density of E-ESBL infections over a 17-year period in the university hospital. Patients with E-ESBL bacteremia had a higher mortality rate, highlighting the importance of continued efforts to control the spread of these multidrug-resistant bacteria in the institution.
EUROPEAN JOURNAL OF CLINICAL MICROBIOLOGY & INFECTIOUS DISEASES
(2022)
Article
Environmental Sciences
Kannan Kamala, Pitchiah Sivaperumal
Summary: The present study aimed to determine the presence of extended spectrum beta lactamase (ESBL) genes in bacteria isolated from fresh exportable fish samples collected in Chennai, India. The study found that out of the 2670 bacterial isolates obtained from 293 fish samples, a significant number (1958 isolates) showed multidrug resistance with ESBL genes such as blaCTX, blaSHV, blaTEM, and blaAmpC. The contamination of fresh fish samples with pathogenic bacteria resistant to multiple antibiotics highlights the need for immediate measures to prevent environmental infectivity and ensure the safety of seafood.
ENVIRONMENTAL POLLUTION
(2023)
Article
Immunology
F. Morales-Leon, A. Opazo-Capurro, C. Caro, N. Lincopan, A. Cardenas-Arias, F. Esposito, V. Illesca, M. L. Rioseco, M. Dominguez-Yevenes, C. A. Lima, H. Bello-Toledo, Gerardo Gonzalez-Rocha
Summary: The convergence of virulence and multidrug-resistant phenotype is reported for the first time in Chile in Klebsiella pneumoniae complex isolates, indicating a clinical problem that requires special attention and continuous surveillance in South America. The detected hypervirulent isolates displayed resistance to broad-spectrum cephalosporins and carried multiple antibiotic-resistance genes, leading to a multidrug-resistant phenotype. In vivo virulence testing confirmed higher virulence of the isolates compared to the control strain.
Article
Infectious Diseases
Antonella Santona, Jose Joao Sumbana, Maura Fiamma, Massimo Deligios, Elisa Taviani, Samuel Elija Simbine, Tomas Zimba, Jahit Sacarlal, Salvatore Rubino, Bianca Paglietti
Summary: ESBL-producing ExPEC, including high-risk lineages, were identified in extraintestinal infections at Maputo Central Hospital. The majority of isolates showed multidrug resistance, with ESBL genes associated with resistance to cefotaxime and ceftazidime. Furthermore, different sequence types were found to carry CTX-M transposition units, potentially facilitating the dissemination of ESBL genes. Genomic analysis revealed a diverse array of virulence-associated genes and clonality among isolates, suggesting the presence of hospital-acquired infections and long-term persistence.
INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS
(2022)
Article
Infectious Diseases
Danny Fuentes-Castillo, Pedro Enrique Navas-Suarez, Maria Fernanda Gondim, Fernanda Esposito, Carlos Sacristan, Herrison Fontana, Bruna Fuga, Camila Piovani, Robert Kooij, Nilton Lincopan, Jose Luiz Catao-Dias
Summary: Antimicrobial-resistant bacteria have been found in wildlife, with little understanding of their impact on wildlife health. This study reports the first fatal colibacillosis caused by a multidrug-resistant ESBL-producing E. coli in critically endangered Brazilian merganser, highlighting the threat of priority pathogens to wildlife. The identified E. coli ST58 clone has been previously found in humans, food-producing animals, wildlife, and the environment, indicating broad adaptation and persistence at the human-animal-environment interface.
TRANSBOUNDARY AND EMERGING DISEASES
(2021)
Article
Microbiology
Christa Ewers, Anno de Jong, Ellen Prenger-Berninghoff, Farid El Garch, Ursula Leidner, Sumeet K. Tiwari, Torsten Semmler
Summary: Livestock animals may serve as a significant source of ESBL/pAmpC E. coli contamination for humans. Analysis of Escherichia spp. isolates from healthy livestock fecal samples revealed a variety of ESBL/pAmpC types, with the majority of genes located on plasmids, indicating the potential dissemination of cephalosporin resistance genes through globally successful plasmid lineages in livestock.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Microbiology
Benti D. Gelalcha, Ruwaa I. Mohammed, Aga E. Gelgie, Oudessa Kerro Dego
Summary: This study investigates the presence of ESBL-producing E. coli and Klebsiella spp. in raw milk from dairy cattle farms. The results show a high prevalence of ESBL-producing bacteria, indicating a potential risk to human health. The detected strains were multidrug resistant and carried various resistance genes and virulence markers. These findings highlight the importance of milk safety and the need for monitoring and control measures.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Infectious Diseases
Antonio Mandujano, Diana Veronica Cortes-Espinosa, Jose Vasquez-Villanueva, Paulina Guel, Gildardo Rivera, Karina Juarez-Rendon, Wendy Lizeth Cruz-Pulido, Guadalupe Aguilera-Arreola, Abraham Guerrero, Virgilio Bocanegra-Garcia, Ana Veronica Martinez-Vazquez
Summary: This study investigated the antimicrobial resistance profile of ESBL-producing E. coli among food-producing animals in Tamaulipas, Mexico. A total of 200 fecal samples were collected and 5.0% of the strains were confirmed as ESBL producers. High percentage of antimicrobial resistance was observed against gentamicin, tetracycline, and streptomycin. The study suggests that food-producing animals may serve as reservoirs and contribute to the spread of ESBL-producing bacteria.
Article
Infectious Diseases
Jungsun Park, Eunkyung Shin, Wooju Kang, Joohyun Han, Jaeil Yoo, Jung-Sik Yoo, Dong-Hyun Roh, Junyoung Kim
Summary: This study characterized ESBL-producing pathogenic E. coli CC10 strains isolated from patients with diarrhea and found that CTX-M-15-producing E. coli O25:H16 isolates were the major type associated with CC10 distribution over the past decade. Continuous monitoring is crucial to prevent the further dissemination of resistant ESBL-producing E. coli CC10 strains.
Article
Public, Environmental & Occupational Health
Ali Qasemi, Fateh Rahimi, Mohammad Katouli
Summary: The prevalence of biofilm and beta-lactamase-producing UPEC strains differed significantly between Isfahan and Zahedan in Iran, with higher rates observed in the city of Zahedan. This suggests that socioeconomic status and environmental factors may play a role in the pathogenicity of these strains.
PATHOGENS AND GLOBAL HEALTH
(2022)
Article
Infectious Diseases
Yewei Fang, Shuan Tao, Huimin Chen, Yao Xu, Luyan Chen, Wei Liang
Summary: This study compared the clonality, virulence gene content, and phylogenetic group differences between non-ESBL-EC and ESBL-EC isolates from urine. The results showed that phylogenetic group B2 was the most prevalent in both non-ESBL-EC and ESBL-EC groups. The presence and count of acquired virulence genes were significantly higher in B2 of both non-ESBL-EC and ESBL-EC, and specific sequence types were found exclusively in each group.
INFECTION AND DRUG RESISTANCE
(2023)
Article
Biochemistry & Molecular Biology
Olivia M. Gruenzweil, Lauren Palmer, Adriana Cabal, Michael P. Szostak, Werner Ruppitsch, Christian Kornschober, Maciej Korus, Dusan Misic, Tanja Bernreiter-Hofer, Anna D. J. Korath, Andrea T. Fessler, Franz Allerberger, Stefan Schwarz, Joachim Spergser, Elke Mueller, Sascha D. Braun, Stefan Monecke, Ralf Ehricht, Chris Walzer, Hrvoje Smodlaka, Igor Loncaric
Summary: The study aimed to investigate the presence of ESBL- and AmpC-producing Enterobacterales, as well as Salmonella, in marine mammals. Results revealed a high prevalence of multidrug-resistant bacteria and pandemic high-risk clones, indicating anthropogenic antimicrobial pollution in these animals.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Immunology
Isabelle Vock, Lisandra Aguilar-Bultet, Adrian Egli, Pranita D. Tamma, Sarah Tschudin-Sutter
Summary: Majority of infections in hospitalized patients colonized with ESBL-PE are not caused by ESBL-PE. Site of infection and patient-level exposures may serve as useful predictors for nESBI,-PE infections, potentially guiding empiric treatment recommendations.
CLINICAL INFECTIOUS DISEASES
(2021)
Article
Microbiology
Chao Li, Xuan Chen, Zijing Ju, Cui Li, Ying Xu, Jiawei Ding, Yuting Wang, Peng Ma, Kui Gu, Changwei Lei, Yizhi Tang, Hongning Wang
Summary: Bacterial drug resistance, especially extended-spectrum β-lactamase-producing E. coli (ESBL-E. coli), is a significant global threat to human health. In this study, 408 ESBL-E. coli strains were isolated from hospitals and chicken farms, and a high genetic similarity was found between some CTX-ESBL-E. coli strains from Sichuan Province and clinical sites, indicating a risk of ESBL-E. coli transmission from chickens to humans. The high-risk clonal strains ST131 and ST1193 primarily carried bla(CTX-M-27) gene, emphasizing the importance of monitoring drug-resistant E. coli from animal and human sources.
MICROBIOLOGY SPECTRUM
(2022)
Article
Microbiology
Elias Eger, Stefan E. Heiden, Katja Korolew, Claude Bayingana, Jules M. Ndoli, Augustin Sendegeya, Jean Bosco Gahutu, Mathis S. E. Kurz, Frank P. Mockenhaupt, Julia Mueller, Stefan Simm, Katharina Schaufler
Summary: This study demonstrates the circulation of clinically relevant, pathogenic ESBL-producing E. coli among patients, caregivers, and the community in Rwanda. These strains combine antimicrobial resistance with virulence and show potential exchange of mobile genetic elements among bacterial pathogens, posing a significant risk worldwide.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Immunology
Iolanda Jordan, Mariona Fernandez de Sevilla, Victoria Fumado, Quique Bassat, Elisenda Bonet-Carne, Claudia Fortuny, Aleix Garcia-Miquel, Cristina Jou, Cristina Adroher, Maria Mele Casas, Monica Girona-Alarcon, Maria Hernandez Garcia, Gemma Pons Tomas, Sara Ajanovic, Sara Arias, Nuria Balanza, Barbara Baro, Pere Millat-Martinez, Rosauro Varo, Sergio Alonso, Enric Alvarez-Lacalle, Daniel Lopez, Joana Claverol, Marta Cubells, Pedro Brotons, Anna Codina, Daniel Cuadras, Patricia Bruijning-Verhagen, Saul Faust, Alasdair Munro, Carmen Munoz-Almagro, Marti Catala, Clara Prats, Juan Jose Garcia-Garcia, Eduard Gratacos
Summary: The transmission rate of SARS-CoV-2 infection among children and adult staff in summer schools in Barcelona was lower than the general population, indicating that schools under strict preventive measures are unlikely to be significant sources of transmission.
CLINICAL INFECTIOUS DISEASES
(2022)
Article
Immunology
Ogechukwu A. Asogwa, Marieke L. A. de Hoog, Patricia C. J. Bruijning-Verhagen
Summary: The introduction of PCV10 in the Netherlands resulted in a significant reduction in the incidence of pneumonia-bronchitis and sinusitis, but the impact on otitis media remains uncertain. Estimates vary depending on the method of analysis used.
Article
Infectious Diseases
Sebastiaan J. van Hal, Rob J. L. Willems, Theodore Gouliouris, Susan A. Ballard, Teresa M. Coque, Anette M. Hammerum, Kristin Hegstad, Mette Pinholt, Benjamin P. Howden, Surbhi Malhotra-Kumar, Guido Werner, Katsunori Yanagihara, Ashlee M. Earl, Katherine E. Raven, Jukka Corander, Rory Bowden
Summary: The study collected 1095 hospital E faecium sequences from 34 countries, augmented by 33 published sequences. It showed that A1 likely emerged as a clone from A2, and that A2 represents a genetic continuum between A1 and community E faecium. The study also found that A2 and B isolates entering the hospital are important reservoirs for ongoing A1 adaptation.
Article
Infectious Diseases
Tess Verschuuren, Thijs Bosch, Valentina Mascaro, Rob Willems, Jan Kluytmans
Summary: The study validated two whole genome sequence-antimicrobial susceptibility testing phenotype prediction tools for Escherichia coli clinical isolates from Dutch routine care, but found that they did not meet FDA criteria for clinical diagnostic use. The tools need further improvement before they can be used for clinical decision making.
CLINICAL MICROBIOLOGY AND INFECTION
(2022)
Article
Microbiology
Ana P. Pereira, Patricia Antunes, Rob Willems, Jukka Corander, Teresa M. Coque, Luisa Peixe, Ana R. Freitas, Carla Novais
Summary: This study explores the activity of chlorhexidine (CHX) against Enterococcus faecalis and the variability of ChlR-EfrEF proteins in diverse populations. The findings suggest that CHX-tolerant isolates are more prevalent in the food chain and recent human infections. The study also provides insights into the genetic diversity of the efrEF operon and its correlation with CHX phenotypes.
MICROBIOLOGY SPECTRUM
(2022)
Article
Infectious Diseases
Eva L. Koekenbier, Konstantin Fohse, Josephine S. van de Maat, Jan Jelrik Oosterheert, Cees van Nieuwkoop, Jacobien J. Hoogerwerf, Martin P. Grobusch, Maurice A. A. J. van den Bosch, Janneke H. H. van de Wijgert, Mihai G. Netea, Frits R. Rosendaal, Marc J. M. Bonten, C. H. (Henri) van Werkhoven, BCG-PRIME Study Grp
Summary: The objective was to test whether BCG vaccination would reduce the incidence of COVID-19 and RTIs in older adults with comorbidities. The results showed that BCG vaccination does not protect older adults against COVID-19, COVID-19 hospitalization, or clinically relevant RTIs.
CLINICAL MICROBIOLOGY AND INFECTION
(2023)
Article
Immunology
Evelien B. van Kempen, Patricia C. J. Bruijning-Verhagen, Dorine Borensztajn, Clementien L. Vermont, Marjolijn S. W. Quaak, Jo-Anne Janson, Ianthe Maat, Kim Stol, Bart J. M. Vlaminckx, Jantien W. Wieringa, Nina M. van Sorge, Navin P. Boeddha, Mirjam van Veen
Summary: After a rise in notifiable invasive group A streptococcal (iGAS) infections in the Netherlands, a survey was conducted among 7 hospitals. Pediatric iGAS cases were twice as high between July 2021 and June 2022 compared to pre-COVID-19. The surge in early 2022 was most notable in children under 5 and for the diagnoses of empyema and necrotizing fasciitis. Investigation and vigilance are necessary for this recent rise in pediatric iGAS cases.
PEDIATRIC INFECTIOUS DISEASE JOURNAL
(2023)
Article
Immunology
Jon Salmanton-Garcia, Pauline Wipfler, Paula Valle-Simon, Christina Merakou, Ioannis Kopsidas, Ullrich Bethe, Angela Steinbach, Orly Spivak, Lenka Souckova, Maria Amelia Mendonca, Markela Koniordou, Margot Hellemans, Jesus Frias-Iniesta, Ruth Joanna Davis, Imre Barta, Anna Maria Azzini, Helena H. Askling, Christos D. Argyropoulos, Elena Alvarez-Barco, Murat Akova, Marc M. J. Bonten, Miriam Cohen-Kandli, Rebecca Jane Cox, Robert Flisiak, Petr Husa, Ligita Jancoriene, Alena Koscalova, Odile Launay, Jens Lundgren, Patrick Mallon, Laura Marques, Pontus Naucler, Jordi Ochando, Zoi-Dorothea Pana, Evelina Tacconelli, Krisztina Toth, Sven Trelle, Pierre van Damme, Theoklis E. Zaoutis, Markus Zeitlinger, Kerstin Albus, Fiona A. Stewart, Sanne H. I. Hofstraat, Patricia Bruijning-Verhagen, Oliver A. Cornely, ACCELERATE Consortium
Summary: The VACCELERATE consortium created a network to accelerate the clinical development of vaccines in Europe. By April 2023, 481 research sites from 39 European countries have registered in the network. The network has been used 21 times for academic and industry trials since its launch in October 2020.
Article
Public, Environmental & Occupational Health
Chiara de Bellegarde de Saint Lary, Louella M. R. Kasbergen, Patricia C. J. L. Bruijning-Verhagen, Henk van der Jeugd, Felicity Chandler, Boris M. Hogema, Hans L. Zaaijer, Fiona R. M. van der Klis, Luisa Barzon, Erwin de Bruin, Quirine ten Bosch, Marion P. G. Koopmans, Reina S. Sikkema, Leo G. Visser
Summary: In 2020, the first cases of Dutch West Nile virus (WNV) infected birds were detected through targeted surveillance. Retrospective testing revealed human WNV infections, indicating the potential risks for bird ringers due to their exposure to mosquito bites and avian excrements. This study found that bird ringers have a higher risk of exposure to WNV and USUV compared to the general population.
Article
Public, Environmental & Occupational Health
Christos Argyropoulos, Janina Leckler, Jon Salmanton-Garcia, Marinos Constantinou, Alexandra Alexandrou, Sophia Themistocleous, Evgenia Noula, George Shiamakkides, Andria Nearchou, Fiona A. Stewart, Kerstin Albus, Markela Koniordou, Ioannis Kopsidas, Orly Spivak, Margot Hellemans, Greet Hendrickx, Ruth Joanna Davis, Anna Maria Azzini, Paula Valle Simon, Antonio Javier Carcas-Sansuan, Helena Hervius Askling, Sirkka Vene, Jana Baranda Prellezo, Elena alvarez-Barco, Alan J. Macken, Romina Di Marzo, Catarina Luis, Ole F. Olesen, Jesus A. Frias Iniesta, Imre Barta, Krisztina Toth, Murat Akova, Marc M. J. Bonten, Miriam Cohen-Kandli, Rebecca Jane Cox, Lenka Souckova, Petr Husa, Ligita Jancoriene, Odile Launay, Jens Lundgren, Patrick Mallon, Charis Armeftis, Laura Marques, Pontus Naucler, Jordi Ochando, Evelina Tacconelli, Pierre van Damme, Theoklis Zaoutis, Sanne Hofstraat, Patricia Bruijning-Verhagen, Markus Zeitlinger, Oliver A. Cornely, Zoi Dorothea Pana
Summary: This study aims to develop a toolkit that provides trustworthy information and promotes positive attitudes towards vaccine trials, with a focus on inclusiveness and equity. The produced materials, including brochures, videos, and puzzles, inform the public about the benefits and risks of trial participation and aim to build confidence in the COVID-19 vaccines.
JMIR PUBLIC HEALTH AND SURVEILLANCE
(2023)
Article
Public, Environmental & Occupational Health
L. Jonker, K. J. Linde, A. R. de Boer, E. Ding, D. Zhang, M. L. A. de Hoog, S. Herfst, D. J. J. Heederik, P. L. A. Fraaij, P. M. Bluyssen, I. M. Wouters, P. C. J. L. Bruijning-Verhagen
Summary: The study aimed to investigate the impact of COVID-19 measures on SARS-CoV-2 incidence in secondary schools and its association with classroom CO2 concentration and airborne contamination. The findings showed that school-initiated COVID-19 measures had no additional effect on the incidence rate. Furthermore, the study found that the incidence rate decreased during the national lockdown and post-lockdown periods compared to the pre-lockdown period.
Article
Virology
Judith G. C. Sluiter-Post, Elandri Fourie, Joanne G. Wildenbeest, Steven F. L. van Lelyveld, Patricia C. J. L. Bruijning-Verhagen, Marianne A. van Houten
Summary: This study aimed to evaluate persisting symptoms in children at 6 and 12 months after a SARS-CoV-2 infection. The findings showed that previously healthy children had rare persistent symptoms, while some uninfected children reported mild symptoms, but there were no significant differences.
Article
Microbiology
Chrispin Chaguza, Anna K. Poentinen, Janetta Top, Sergio Arredondo-Alonso, Ana R. Freitas, Carla Novais, Carmen Torres, Stephen D. Bentley, Luisa Peixe, Teresa M. Coque, Rob J. L. Willems, Jukka Corander
Summary: This study used a genome-wide association analysis to investigate the genetic changes in Enterococcus faecalis isolates and their association with hospitalization status and body isolation source. The results showed that infection by hospitalization status and extraintestinal infection are heritable traits, but no clear individual genetic changes were found to be associated with hospitalization status or body isolation source. The study also identified a genomic region potentially linked to infection.
MICROBIOLOGY SPECTRUM
(2023)
Article
Microbiology
Janetta Top, Tess D. Verschuuren, Marco C. Viveen, M. Eugenia Riccio, Stephan Harbarth, Jan A. J. W. Kluytmans, Rob J. L. Willems, Fernanda L. Paganelli
Summary: This study investigated the gut microbiome dynamics of patients colonized with ESBL-Ec or ESBL-Kp and their household contacts. The results showed that the microbiome composition of index patients was different from that of household contacts, but in some patients, the microbiome gradually recovered to a healthier state. The microbiome composition of household contacts remained stable over time, regardless of colonization with ESBL positive bacteria.
MICROBIOLOGY SPECTRUM
(2023)
Article
Microbiology
Ana P. Pereira, Patricia Antunes, Paula Bierge, Rob J. L. Willems, Jukka Corander, Teresa M. Coque, Oscar Q. Pich, Luisa Peixe, Ana R. Freitas, Carla Novais
Summary: Quaternary ammonium compounds (QACs) are widely used in various settings, while Enterococcus faecalis and Enterococcus faecium are recognized as nosocomial pathogens. This study evaluated the susceptibility of Enterococcus to QACs and found that they rarely carry related tolerance genes, but their susceptibility can decrease under specific environmental conditions.
MICROBIOLOGY SPECTRUM
(2023)
