期刊
BIOSENSORS & BIOELECTRONICS
卷 64, 期 -, 页码 74-80出版社
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2014.08.049
关键词
Double-stranded target; DNA methylation; Hybridization kinetics; Electrochemical impedance spectroscopy
类别
资金
- Ministry of Business, Innovation and Employment [UOAX1201]
- New Zealand Ministry of Business, Innovation & Employment (MBIE) [UOAX1201] Funding Source: New Zealand Ministry of Business, Innovation & Employment (MBIE)
In this communication we report on two important effects related to the detection of DNAs. Firstly, we investigate the sensor response to target DNA when the target is in a double stranded (ds) form and compare the response to single stranded (ss) target DNA. The importance in evaluating such an effect lies in the fact that most biological DNA targets are found in ds form. Secondly, we use synthetic ds targets to investigate the effect of DNA methylation on the sensor response. DNA methylation is known to affect functional properties of DNA and is related to a number of diseases, including various cancers. In these studies, we utilize our previously developed sensor platform, which is based on the use of a glassy carbon electrode-confined conducting polymer that is covalently modified with DNA probe sequences. The signal detection methodology we use is measuring a change in the reaction kinetics of ferro-ferricyanide redox couple at the electrode upon hybridization by means of electrical impedance spectroscopy (EIS). Additionally, EIS is utilized to study the kinetics of the hybridization of the conducting polymer-bound probe with methylated vs. non-methylated ds-DNA. Preliminary results are proving valuable as a guide to the future design of sensors for gene methylation. (C) 2014 Elsevier B.V. All rights reserved.
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