期刊
BIOSENSORS & BIOELECTRONICS
卷 74, 期 -, 页码 687-690出版社
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2015.06.070
关键词
Electrochemical sensor; Double-stranded DNA; Hybridization chain reaction; Positively charged gold nanoparticles
类别
资金
- National Natural Science Foundation of China [21305053, 21375153]
- Natural Science Fund for Colleges and Universities in Jiangsu Province [13KJB150015]
- Natural Science Fund in Jiangsu Province [BK20130227]
- Jiangsu Normal University, China [12XLR022]
Enhanced sequence-specific recognition of double-stranded DNA (dsDNA) was realized by using hybridization chain reaction (HCR) and positively charged gold nanoparticles ((+)AuNPs) dual signal amplification. To construct such a sensor, capture probe was initially assembled onto gold electrode surface. Upon addition of dsDNA, sandwiched DNA complex was formed between the capture probe and the detection probe, then another exposed part of the detection probe opened two alternating DNA hairpins (H-1 and H-2) in turn and initiated HCR to form a double-helix. Meantime, (+)AuNPs were electrostatically adsorbed onto such double-helix to amplify the electrochemical signal. Upon optimal conditions, the electronic signals of ferrocene (Fc) that modified on H1 and H2 increased linearly with increasing dsDNA concentration over the range from 15 pM to 1.0 nM, with a detection limit of 2.6 pM. Moreover, the proposed method showed good sequence specificity for dsDNA recognition. (C) 2015 Elsevier B.V. All rights reserved.
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