期刊
PATHOLOGY RESEARCH AND PRACTICE
卷 216, 期 2, 页码 -出版社
ELSEVIER GMBH
DOI: 10.1016/j.prp.2019.152786
关键词
miR-593-5p; PLK1; non-small cell lung cancer cells
类别
资金
- National Natural Science Foundation of China [81601380, 81802651, 81702275]
- natural science research project in colleges and universities of Anhui province [KJ2018A0269, KJ2019A0409]
- Key Research Foundation of Wannan Medical College [WK2019Z7]
- Foundation of non-coding RNA transformation of major diseases of Anhui key universities [RNA201910]
- Foundation of Peak Training Program for Scientific Research of Yijishan Hospital, Wannan Medical College [GF2019G11]
- Foundation for the cultivation of outstanding and top talents in Colleges and universities of Anhui Province [gxgnfx2019010]
Worldwide, lung cancer has the highest rates of mortality and morbidity, with the majority of its pathology attributable to non-small cell lung cancer (NSCLC). MicroRNAs are pivotal in the occurrence and development of cancer. However, the role of miRNA-593-5p in the progression of NSCLC is not clear. In this study, we investigate, in vitro, whether miRNA-593-5p inhibits NSCLC cell proliferation. To clarify its specific mechanism of inhibition, we used bioinformatics to predict its target genes and identified PLK1. Luciferase reporter assay confirmed the binding of miR-593-5p to the PLK1 3'-UTR in a sequence-specific manner in NSCLC cells. Additionally, we also found through Western blot and quantitative RT-PCR that miR-593-5p down-regulates the expression of PLK1 protein. Finally, PLK1 overexpression was shown to disinhibit NSCLC cell proliferation. Taken together, this evidence suggests that miR-593-5p inhibits NSCLC cell proliferation by inhibiting PLK1 expression.
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