Article
Endocrinology & Metabolism
Xiangfeng Chen, Bin Wu, XiaoRong Shen, Xin Wang, Ping Ping, Maohua Miao, Ningning Liang, Huiyong Yin, Huijuan Shi, Jun Qian, Tiancheng Zhang
Summary: This study aimed to investigate the effects of PUFA-derived metabolites in seminal plasma on male fertility and evaluate the potential of PUFA as a biomarker for normozoospermic male infertility. Semen samples were collected from 564 men, and the levels of PUFA-derived metabolites were analyzed. The results showed significant differences in the concentrations of certain metabolites between fertile and infertile men with normozoospermia and oligoasthenozoospermia. The metabolites 7(R)-MaR1, 11,12-DHET, 17(S)-HDHA, LXA5, and PGJ2 were identified as potential diagnostic biomarkers for infertility in normozoospermic men.
FRONTIERS IN ENDOCRINOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Luca Frattaruolo, Miriana Durante, Maria Stella Cappello, Anna Montefusco, Giovanni Mita, Anna Rita Cappello, Marcello Salvatore Lenucci
Summary: Supercritical fluid extraction with CO2 (SFE) and conventional solvent extraction (CSE) were used to extract food-grade bioactives from carrot and pumpkin, and their ability to counteract inflammation and oxidative reactions was evaluated. The extracts showed dose-dependent anti-inflammatory effects and interfered with pro-inflammatory effects of breast cancer cells and macrophages. The involvement of Nuclear factor-erythroid-2-related factor 2 (Nrf2) in these reactions was observed. The concentrations of reactive oxygen species (ROS) and nitrite production (NP) were negatively correlated with alpha-tocopherol and most carotenoids, suggesting the importance of extract constituents' interactions.
Review
Biochemistry & Molecular Biology
Jenny Leopold, Patricia Prabutzki, Kathrin M. Engel, Juergen Schiller
Summary: The occurrence of free fatty acids and reactive oxygen species is characteristic of inflammatory diseases. Unsaturated fatty acids react with reactive oxygen species, producing important products. These modified fatty acids can be released from phospholipids or oxidatively modified, and oligomeric products may be formed. Hydroxy fatty acid esters are considered important products, and there are different opinions on the role of dimeric fatty acids.
Review
Veterinary Sciences
Chongshan Yuan, Jun Wang, Wenfa Lu
Summary: Fatty acids have different types and play an important role in maintaining semen quality. This review focuses on the regulation of fatty acids, diet, and extender on semen quality, and their effects on sperm motility, plasma membrane integrity, DNA integrity, hormone content, and antioxidant capacity. Future research should analyze the fatty acid profiles of different species or different periods of the same species and explore suitable methods, doses, and mechanisms of regulating semen quality.
FRONTIERS IN VETERINARY SCIENCE
(2023)
Review
Biochemistry & Molecular Biology
Qing Wei Calvin Ho, Xiaofeng Zheng, Yusuf Ali
Summary: Ceramides, a class of sphingolipids, play a role in various skin disorders and metabolic diseases. Recent research has focused on identifying specific ceramide species and their effects on different diseases, and has found ceramides linked to metabolic dysfunction and cardiac decline.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Chemistry, Applied
B. Pretorius, H. C. Schonfeldt
Summary: This study aimed to investigate the impact of offal on fatty acid composition and lipid quality, finding that offal can provide beneficial fatty acids for the diet.
Article
Obstetrics & Gynecology
Sarah Z. Gavrizi, Pardis Hosseinzadeh, Richard Steven Brush, Madison Tytanic, Erin Eckart, Jennifer D. Peck, LaTasha B. Craig, Michael P. Diamond, Martin-Paul Agbaga, Karl R. Hansen
Summary: This study aims to explore the correlation between VLC-PUFAs levels and sperm parameters, as well as the outcome of live birth after conventional therapy for unexplained infertility. The results demonstrate a positive association between VLC-PUFAs percentage and sperm concentration, motility, morphology, and total motile count. Moreover, a significant link is observed between hydroxylated VLC-PUFA percentage and the likelihood of a live birth outcome.
FERTILITY AND STERILITY
(2023)
Article
Pharmacology & Pharmacy
Haining Yu, Chengjie Fang, Peng Li, Manman Wu, Shengrong Shen
Summary: The study found that gut microbiome dysbiosis caused abnormal lipid metabolism, and DHA was able to repair the lipid metabolism shifts resulting from gut microbiome dysbiosis. DHA could modulate host-gut microbiome signatures, improve concentrations of SCFAs, regulate fatty acids metabolism, and modify bile acid profiles.
EUROPEAN JOURNAL OF PHARMACOLOGY
(2021)
Article
Biology
Yehui Duan, Changbing Zheng, Jie Zheng, Lu Ma, Xinran Ma, Yinzhao Zhong, Xichen Zhao, Fengna Li, Qiuping Guo, Yulong Yin
Summary: This study evaluated the composition of amino acids, fatty acids, and metabolic profiles in the longissimus thoracis muscle of Shaziling pigs at different ages. The results showed that the predominant fatty acids in the muscle were C16:0, C16:1, C18:0, C18:1n9c, and C18:2n6c. Alanine, aspartate, glutamate, D-glutamine, and D-glutamate metabolism were identified as the main metabolic pathways for the meat flavor. Additionally, anserine, C16:0, C16:1, and C18:1n9c were found to be correlated with intramuscular fat, pH(24h), L* (lightness), and b* (yellowness) values.
SCIENCE CHINA-LIFE SCIENCES
(2023)
Review
Biochemistry & Molecular Biology
Sylwia Judycka, Joanna Nynca, Piotr Hliwa, Andrzej Ciereszko
Summary: This article presents recent knowledge regarding sex-reversed females (SRFs) of salmonid fish, including sex reversal methods, effects on reproductive tract morphology and semen quality factors, as well as methods for short-term and cryopreservation semen storage. The need for artificial maturation of SRF sperm and recent progress in efficient cryopreservation procedures and use of cryopreserved semen in hatchery practice are discussed. Additionally, proteomic investigations of salmonid sperm, primarily comparing normal male and SRF testicular semen, and changes in SRF rainbow trout sperm proteome after in vitro incubation in artificial seminal plasma are addressed.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Nutrition & Dietetics
Erin D. Clarke, Jordan Stanford, Jessica J. A. Ferguson, Lisa G. Wood, Clare E. Collins
Summary: This study investigates the relationship between fatty acids, diet quality scores, and inflammatory markers. The results indicate a positive association between saturated fat and inflammation, while monounsaturated fatty acids, polyunsaturated fatty acids, and the Mediterranean diet are inversely associated with inflammation.
Review
Biochemistry & Molecular Biology
Giulia Collodel, Elena Moretti, Daria Noto, Roberta Corsaro, Cinzia Signorini
Summary: This review examines the role of fatty acids in human pathological conditions, particularly infertility. Fatty acids and their derivatives play important roles in regulating cell membrane composition, cell signaling, cell death, immune responses, and inflammation. Understanding the metabolism of fatty acids can lead to new approaches in treating infertility.
Article
Multidisciplinary Sciences
A. J. Crean, S. Afrin, H. Niranjan, T. J. Pulpitel, G. Ahmad, A. M. Senior, T. Freire, F. Mackay, M. A. Nobrega, R. Barres, S. J. Simpson, T. Pini
Summary: Dietary factors have an influence on male reproductive function, and the Nutritional Geometry framework was used to study the effects of macronutrient balance on reproductive traits in male mice. The study found that different dietary compositions have varying effects on morphological, testicular, and sperm traits. Interestingly, dietary fat was found to have a positive influence on sperm motility and antioxidant capacity. Body adiposity, on the other hand, was not significantly correlated with any of the reproductive traits measured.
NATURE COMMUNICATIONS
(2023)
Review
Biodiversity Conservation
Ana M. M. Goncalves, Carolina P. Rocha, Joao C. Marques, Fernando J. M. Goncalves
Summary: The toxic effects of pesticides, especially in aquatic ecosystems, have been a major concern for environmental and human safety. Biomarkers, such as antioxidant enzymes and fatty acids, are crucial in assessing the impacts of pesticides on organisms. Fatty acids have been identified as potential biomarkers due to their sensitivity to alterations in organism homeostasis, particularly in freshwater communities.
ECOLOGICAL INDICATORS
(2021)
Article
Reproductive Biology
Jones Ofosu, Moses Addo Nartey, Xianhong Mo, Jiangfeng Ye, Yan Zhang, Changjun Zeng, Ming Zhang, Yi Fang, Guangbin Zhou
Summary: Metabolites in ram sperm play important roles in maintaining plasma membrane integrity, energy metabolism, and cryotolerance regulation. This study identified differential metabolites during cryopreservation in ram sperm and found that certain key polyunsaturated fatty acids were down-regulated during cooling and cryopreservation. The differentially regulated metabolites were enriched in several metabolic pathways, providing new knowledge to improve the cryopreservation process.
Article
Infectious Diseases
Giulio Curone, Filippo Biscarini, Elisa Cotozzolo, Laura Menchetti, Alessandro Dal Bosco, Federica Riva, Paola Cremonesi, Stella Agradi, Simona Mattioli, Bianca Castiglioni, Alessia Di Giancamillo, Alice Cartoni Mancinelli, Susanna Draghi, Alda Quattrone, Giulia Collodel, Silvia Clotilde Modina, Cesare Castellini, Gabriele Brecchia
Summary: The study evaluated the effects of two dietary sources of n-3 polyunsaturated fatty acids (PUFAs) on the gut microbiota, caecal fermentations, and gastrointestinal histology in rabbits. Results showed that n-3 PUFAs supplementation led to an increase in beneficial bacterial populations and reduced Bacilli abundance and Firmicutes/Bacteroidetes ratio. These findings suggest that n-3 PUFAs diet supplementation may have positive effects on gut health, although appropriate dosage should be further investigated.
Article
Biochemistry & Molecular Biology
Simona Mattioli, Corrado Dimauro, Alberto Cesarani, Alessandro Dal Bosco, Desiree Bartolini, Francesco Galli, Anna Migni, Bartolomeo Sebastiani, Cinzia Signorini, Camille Oger, Giulia Collodel, Cesare Castellini
Summary: The study aimed to develop a dynamic model using rabbits as a model to evaluate the balance between reactive oxygen species, polyunsaturated fatty acids, and antioxidants. The model showed good accuracy in predicting the effects of dietary treatments on the redox homeostasis of rabbits.
Review
Biochemistry & Molecular Biology
Giulia Collodel, Elena Moretti, Daria Noto, Roberta Corsaro, Cinzia Signorini
Summary: This review examines the role of fatty acids in human pathological conditions, particularly infertility. Fatty acids and their derivatives play important roles in regulating cell membrane composition, cell signaling, cell death, immune responses, and inflammation. Understanding the metabolism of fatty acids can lead to new approaches in treating infertility.
Article
Immunology
Elena Moretti, Cinzia Signorini, Daria Noto, Sergio Antonio Tripodi, Andrea Menchiari, Ester Sorrentino, Giulia Collodel
Summary: This study explored the relationships among omentin-1/ITLN1, semen parameters, and oxidative stress markers in different patient groups. The levels of omentin-1/ITLN1 were found to be correlated with F-2-IsoP levels and negatively correlated with sperm parameters. Infertile patients showed lower levels of omentin-1/ITLN1, while those with varicocele and genitourinary infections had higher levels. Omentin-1/ITLN1 was localized differently in sperm and male reproductive system tissues.
JOURNAL OF INFLAMMATION RESEARCH
(2022)
Article
Biochemistry & Molecular Biology
Giulia Collodel, Elena Moretti, Daria Noto, Roberta Corsaro, Cinzia Signorini, Claudia Bonechi, Lorenzo Cangeloni, Giovanni Luca, Iva Arato, Francesca Mancuso
Summary: This study aimed to investigate the effects of different types of liposomes on porcine prepubertal Sertoli cells (SCs) and to find a suitable carrier for in vitro testing of drugs. The results showed that anionic and cationic liposomes altered the distribution of actin and vimentin filaments and increased the phosphorylation levels of AMPK alpha. Zwitterionic liposomes, on the other hand, did not cause any changes in the cell cytoplasm. These preliminary data suggest that zwitterionic liposomes are the most suitable carrier for in vitro studies of SCs to understand the effects of molecules or drugs with potential clinical applications in the treatment of certain forms of male infertility.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Giulia Collodel, Laura Gambera, Anita Stendardi, Fabiola Nerucci, Cinzia Signorini, Caterina Pisani, Marzia Marcheselli, Francesca Letizia Vellucci, Silvana Enrica Pizzasegale, Lucia Micheli, Elena Moretti
Summary: This study investigated the changes and relationships of various molecules in the follicular fluid (FF) of infertile women with different reproductive conditions. It found that some biochemical components, hormones, antioxidant enzymes, F-2-Isoprostanes (F-2-IsoPs), and resolvin (Rv) D1 in the FF are associated with follicular growth, oocyte maturation, and female infertility. These findings highlight the importance of personalized therapeutic approaches and optimized assisted reproductive techniques (ART) outcomes.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Medicine, General & Internal
Fraide A. Ganotice Jr, Xiaoai Shen, Jacqueline Kwan Yuk Yuen, Yin Man Amy Chow, Anita M. Y. Wong, Karen M. K. Chan, Binbin Zheng, Linda Chan, Pauline Yeung Ng, Siu Chung Leung, Elizabeth Barrett, Hoi Yan Celia Chan, Wing Nga Chan, Kit Wa Sherry Chan, Siu Ling Polly Chan, So Ching Sarah Chan, Esther W. Y. Chan, Yuet Ying Jessica Cheuk, Jacky Choy, Qing He, Julienne Jen, Jingwen Jin, Ui Soon Khoo, Ho Yan Angie Lam, May P. S. Lam, Yik Wa Law, Jetty Chung Yung Lee, Feona Chung Yin Leung, Ann Leung, Rebecca K. W. Liu, Vivian Wei Qun Lou, Pauline Luk, Zoe Lai Han Ng, Alina Yee Man Ng, Maggie Wai Ming Pun, Mary Lok Man See, Jiangang Shen, Grace Pui Yuk Szeto, Eliza Y. T. Tam, Winnie Wan Yee Tso, Ning Wang, Runjia Wang, Janet Kit Ting Wong, Janet Yuen Ha Wong, Grace Wai Yee Yuen, George Lim Tipoe
Summary: This study conducted a quantitative research on 925 healthcare students from two institutions in Hong Kong and used the Social Interaction Anxiety Scale (SIAS-6) and Social Phobia Scale (SPS-6). The results showed that these scales have good psychometric properties among healthcare students in Hong Kong. The study also proposed quantitative, qualitative, and mixed methods research designs to guide researchers in contributing to the discussion of student's social interactions in IPE.
ANNALS OF MEDICINE
(2023)
Article
Biochemistry & Molecular Biology
Susanne Csader, Marsena Jasiel Ismaiah, Tiina Kuningas, Merja Heinaniemi, Janne Suhonen, Ville Mannisto, Heikki Pentikainen, Kai Savonen, Milla-Maria Tauriainen, Jean-Marie Galano, Jetty Chung-Yung Lee, Reeta Rintamaki, Piia Karisola, Hani El-Nezami, Ursula Schwab
Summary: Exercise intervention without weight loss may influence the AT morphology and fat metabolism at the gene expression level in female NAFLD subjects.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Education & Educational Research
Fraide Ganotice, Binbin Zheng, Pauline Yeung Ng, Siu Chung Leung, Elizabeth Ann Barrett, Hoi Yan Celia Chan, Chad W. N. Chan, Kit Wa Sherry Chan, Linda Chan, M. K. Karen Chan, Siu Ling Polly Chan, So Ching Sarah Chan, Esther W. Y. Chan, Julie Chen, Yuet Ying Jessica Cheuk, Yin Kei Doris Chong, Yin Man Amy Chow, Kwok Pui Jody Chu, Hon Yin Brian Chung, Shun Yee Amy Ho, Julienne Jen, Jingwen Jin, Ui Soon Khoo, Ho Yan Angie Lam, May P. S. Lam, Suk Fun Veronica Lam, Pamela Pui-Wah Lee, Jetty Chung-Yung Lee, Chung Yin Feona Leung, Anna K. Y. Leung, Xiang Lin, Rebecca K. W. Liu, Wei Qun Vivian Lou, Pauline Luk, Lai Han Zoe Ng, Yee Man Alina Ng, Tin Wai Terry Ng, Lok Man Mary See, Jiangang Shen, Xiaoai Shen, Grace Szeto, Eliza Y. T. Tam, Kelvin Kai-Wang To, Wan-Yee Winnie Tso, Dana Vackova, Ning Wang, Runjia Wang, Hoi Yan Gloria Wong, K. T. Janet Wong, M. Y. Anita Wong, Yuen Ha Janet Wong, Kwan Yuk Jacqueline Yuen, Wai Yee Grace Yuen, Mine Orlu, George L. Tipoe
Summary: This study investigates the importance of external partnerships in interprofessional education and evaluates the effectiveness of the program. Through data collection and analysis, the researchers identify factors in establishing global joint education programs and significant changes in students' performance in teamwork, positive professional identity, roles, and responsibilities.
BMC MEDICAL EDUCATION
(2023)
Review
Biochemistry & Molecular Biology
Elena Moretti, Cinzia Signorini, Roberta Corsaro, Maria Giamalidi, Giulia Collodel
Summary: Spermatozoa produce reactive oxygen species (ROS) due to aerobic metabolism, which can have both beneficial and detrimental effects on cell function. Assisted reproductive procedures, such as cryopreservation, can lead to excessive ROS production and oxidative damage to spermatozoa. This review focuses on using human sperm as an in vitro model to study the supplementation of antioxidants in media. The presence and synergic effects of different antioxidant molecules may lead to more effective products in vitro and potentially in vivo.
Article
Education & Educational Research
Tracy X. P. Zou, King L. Chow, Tom J. Barry, Jetty Chung-Yung Lee, Ka Sing Yu
Summary: Undergraduate research practices in different curricula were explored through a multiple-case study conducted in two research-intensive universities in Hong Kong. Five approaches to undergraduate research were identified, including project-based, process-based, lab-based, scenario-based, and research community-based approaches. This study also revealed nuanced differences in the adoption of these approaches by faculty members and highlighted the importance of recognizing the research-teaching nexus at both departmental and personal levels.
STUDIES IN HIGHER EDUCATION
(2023)
Article
Biochemistry & Molecular Biology
Simona Mattioli, Elena Moretti, Cesare Castellini, Cinzia Signorini, Roberta Corsaro, Elisa Angelucci, Giulia Collodel
Summary: This study investigated the association of apelin and resolvin (RvD1) in the testis and sperm with the oxidative status of semen and serum testosterone in rabbits fed different diets enriched with flaxseed or fish oil. The results showed that flaxseed diet increased the levels of apelin in the testis and enhanced serum testosterone. Both enriched diets showed higher levels of malondialdehyde and RvD1 in the testis. Apelin and its receptor were also detected in ejaculated sperm.
Article
Biochemistry & Molecular Biology
Emily Kwun Kwan Lo, Xiuwan Wang, Pui-Kei Lee, Ho-Ching Wong, Jetty Chung-Yung Lee, Carlos Gomez-Gallego, Danyue Zhao, Hani El-Nezami, Jun Li
Summary: This study found that Zearalenone (ZEA) intervention can promote the progression of colon cancer by enhancing the BEST4/AKT/ERK1/2 pathway, lowering circulatory amino acid concentrations, altering gut microbiota composition, and suppressing short chain fatty acids production through comprehensive analysis of xenograft mice.
COMPUTATIONAL AND STRUCTURAL BIOTECHNOLOGY JOURNAL
(2023)
Article
Biochemistry & Molecular Biology
Elena Moretti, Cinzia Signorini, Daria Noto, Roberta Corsaro, Lucia Micheli, Thierry Durand, Camille Oger, Jean Marie Galano, Giulia Collodel
Summary: After incubation with F-4-NeuroPs, selected human sperm showed increased motility, membrane mitochondrial potential, and reacted acrosome percentage. Chromatin integrity did not differ between treated and control samples. PSA staining highlighted a significantly higher number of sperm with reacted acrosomes in the treated group after 4 hours. Immunofluorescence analysis revealed a dense distribution of ryanodine receptors in the midpiece and postacrosomal sheath of sperm after 2 hours, followed by a dotted distribution after 4 hours. F-4-NeuroPs at an appropriate concentration can induce sperm capacitation via ion channels, which may enhance the success of assisted fertilization.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Meeting Abstract
Chemistry, Applied
Jetty Chung-Yung Lee, Jean-Marie Glanao, Thierry Durand
JOURNAL OF THE AMERICAN OIL CHEMISTS SOCIETY
(2022)
