Article
Chemistry, Analytical
Hayati Filik, Asiye Aslihan Avan, Nilay Altas Puntar, Mustafa Ozyurek, Zeynep Banu Gungor, Mine Kucur, Handan Kamis, Diclehan Ali Dicle
Summary: A sensitive sandwich-model immunoassay was designed for simultaneous electrochemical measurement of alphafetoprotein and carcinoembryonic antigen using multiple-label strategy. The immunoassay showed good linear relationship and low detection limits for both analytes. The sandwich-type immunosensor was successfully applied to real serum sample analysis.
JOURNAL OF ELECTROANALYTICAL CHEMISTRY
(2021)
Article
Chemistry, Analytical
Dandan Song, Jing Zheng, Nosang Myung, Jingli Xu, Min Zhang
Summary: A sandwich-type electrochemical immunosensor was developed for the detection of CEA, with excellent sensing performance and low detection limit. The magnetic assembly of nanocomposites simplifies further modification process, improving the reliability and reproducibility of the sensor.
Article
Environmental Sciences
Peng Huang, Lingzhang Meng, Jun Pang, Haiting Huang, Jing Ma, Linlin He, Parnian Amani
Summary: This study successfully developed a label-free electrochemical immunosensor based on a metal-organic framework (MOF)-graphene oxide nanocomposite for the detection of carcinoembryonic antigen (CEA), a tumor marker in lung cancer for early cancer diagnosis. The immunosensor showed improved sensitivity, stability, and selectivity, and demonstrated satisfactory accuracy in detecting CEA in human serum samples. These findings suggest that the prepared CEA immunosensor has potential for clinical applications and analysis of human fluids.
ENVIRONMENTAL RESEARCH
(2023)
Article
Biochemistry & Molecular Biology
Zhe Zhao, Ping Wang, Feng Tang, Yao Wang, Shujun Wang, Qing Liu, Yueyun Li
Summary: Here, an innovative sandwich-type electrochemical immunosensor based on multi-order Rubik's cube-type platinum nickel nanocubes and Au NPs/cPDA NTs was developed for ultrasensitive detection of CEA. The immunosensor exhibited a broad linear range, low detection limit, and excellent selectivity, reproducibility, and stability.
BIOELECTROCHEMISTRY
(2023)
Article
Environmental Sciences
Hongli Yang, Zaihua Zhang, Xiaohong Zhou, Norhefozge Binbr Abe Menen, Omid Rouhi
Summary: In this study, electrochemical immunosensing for monitoring carcinoembryonic antigen (CEA) was successfully achieved by immobilizing the antibody on a thionine, chitosan, or graphene oxide nanocomposite modified electrode. The resulting immunosensor showed exceptional sensitivity and selectivity towards CEA compounds, with a low detection limit of 0.8 pg/mL. The application of the immunosensor in detecting CEA in human serum samples demonstrated high accuracy and consistency with ELISA results.
ENVIRONMENTAL RESEARCH
(2023)
Article
Chemistry, Analytical
Xing-Pei Liu, Jing-Shuai Chen, Chang-Jie Mao, Bao-Kang Jin
Summary: A label-free photoelectrochemical immunosensor based on a g-C3N4/CdSe nanocomposite was successfully established for detecting carcinoembryonic antigen (CEA). The sensor showed excellent selectivity and repeatability for CEA detection with a detection limit of 0.21 ng mL(-1) and the potential to detect other protein targets.
Article
Chemistry, Analytical
Wenbo Lu, Xuebo Liu, Huanhuan Shi, Shoufeng Xue, Cancan Bao
Summary: In this study, an ultrasensitive sandwich photoelectrochemical immunosensor for the detection of carcinoembryonic antigen (CEA) was developed. The sensor exhibited a low detection limit, a wide linear range, and excellent specificity, stability, and reproducibility.
MICROCHEMICAL JOURNAL
(2024)
Article
Biophysics
Jin Wang, Jiali Bei, Xu Guo, Yue Ding, Tingting Chen, Bing Lu, Yang Wang, Yukou Du, Yong Yao
Summary: In this study, an ultrasensitive and specific photoelectrochemical (PEC) immunosensor was developed for the detection of carcinoembryonic antigen (CEA). The developed PEC immunosensor demonstrated high specificity, good stability, and excellent reproducibility, making it a promising tool for biomarker immunosensing.
BIOSENSORS & BIOELECTRONICS
(2022)
Article
Biophysics
Jin Wang, Jiali Bei, Xu Guo, Yue Ding, Tingting Chen, Bing Lu, Yang Wang, Yukou Du, Yong Yao
Summary: An ultrasensitive and specific photoelectrochemical immunosensor has been developed for the detection of carcinoembryonic antigen (CEA). The immunosensor exhibits high specificity, good stability, and a sensitive linear response within the detection range.
BIOSENSORS & BIOELECTRONICS
(2022)
Article
Thermodynamics
Alireza Hassanjani Roushan, Abdollah Omrani
Summary: The successful synthesis of NaX nanocrystals using an organic, additive-free hydrothermal approach was reported in this paper. The NaX nanocrystals were found to act as an effective nucleating agent in the crystallization of PVA, enhancing the crystallinity degree of the nanocomposite. The effective energy barrier for the PVA/NaX nanocrystals system was shown to be smaller than that of neat PVA using the Friedman isoconversional method.
JOURNAL OF THERMAL ANALYSIS AND CALORIMETRY
(2021)
Article
Chemistry, Analytical
Yuan-Yuan Tan, Hong-Sheng Tan, Mingjun Liu, Shan-Shan Li
Summary: In order to prevent and diagnose carcinoembryonic antigen (CEA) at an early stage, it is necessary to develop more accurate detection methods. This study constructed a new sensitive electrochemical ratiometric immunosensor for CEA determination using chitosan-ferrocenecarboxaldehyde-gold nanospheres (ChiFc-Au) and reduced graphene oxide-Pd@Au nanocomposites-thionine (rGO-Pd@Au-Thi). The immunosensor utilized dual-signal output to generate two electrical signals (IFc and IThi) that were positively correlated with CEA concentration. Compared to other immunosensors, the proposed immunosensor exhibited a wider linear range and a lower detection limit, making it suitable for CEA measurement in human serum samples.
SENSORS AND ACTUATORS B-CHEMICAL
(2023)
Article
Chemistry, Analytical
Suntisak Khumngern, Panote Thavarungkul, Proespichaya Kanatharana, Tanan Bejrananda, Apon Numnuam
Summary: In this study, an electrochemical sensor based on MIP was developed by modifying a screen-printed carbon electrode with PEDOT:PSS@Fc and a MIP layer. The sensor successfully detected the concentration of PSA.
MICROCHEMICAL JOURNAL
(2022)
Article
Engineering, Electrical & Electronic
Sana Rauf, Hafiz Imran Ahmad Qazi, Jingting Luo, Chen Fu, Ran Tao, Muhammad Sohail, Lei Yang, Honglang Li
Summary: In this study, a highly sensitive sandwich-type quartz crystal microbalance (QCM) immunosensor was developed for the quantitative monitoring of alpha-fetoprotein (AFP), a representative tumor marker for hepatocellular carcinoma. A nanohybrid of cuprous oxide @ molybdenum disulfide (Cu2O@MoS2) combined with gold nanoparticles was synthesized to increase the mass loading on the QCM electrode's surface. The sensor's sensitivity was further enhanced by gold enhancement and a self-assembled monolayer of primary antibody (Ab(1)) was fabricated on the QCM electrode. The immunosensor achieved a detection limit of 35 pg/mL with gold enhancement and 90 pg/mL without gold enhancement.
IEEE SENSORS JOURNAL
(2022)
Article
Biochemistry & Molecular Biology
Chatrawee Direksilp, Napa Parinyanitikul, Nuttha Ariyasajjamongkol, Anuvat Sirivat
Summary: A label-free electrochemical immunosensor composed of thiolated chitosan (tCHI) and doped poly(N-methylaniline) (dPNMA) was developed for determining the concentration of carcinoembryonic antigen (CEA). The tCHI served as a support matrix for immobilizing CEA antibodies (anti-CEA), while the dPNMA provided excellent electrical conductivity as an electron transfer layer. The immunosensor demonstrated a broad concentration range of 0.01 to 30 ng mL-1 and a low limit of detection (LOD) of 0.01 ng mL-1, along with excellent selectivity, reproducibility, and long-term stability.
BIOELECTROCHEMISTRY
(2023)
Article
Chemistry, Analytical
Qian Luo, Xiaomin Qian, Xiaona Mi, Yifeng Tu
Summary: A new type of immunosensor based on a gold nanoparticles/aminozeolitic imidazolate framework-67 nanocomposite was developed for the detection of NT-proBNP, a biomarker for heart failure. The immunosensor showed excellent response and sensitivity, with a broad linear range and low detection limit. It also demonstrated good specificity, stability, and reproducibility, making it suitable for detecting NT-proBNP in real blood samples.
JOURNAL OF ELECTROANALYTICAL CHEMISTRY
(2022)
Article
Biophysics
Reza Khodadadi, Manouchehr Eghbal, Hamideh Ofoghi, Alireza Balaei, Ali Tamayol, Karen Abrinia, Amir Sanati-Nezhad, Mohamadmahdi Samandari
Summary: This paper introduces an integrated portable centrifugal microfluidic system that automates cell and fluid manipulation for complete blood counting (CBC) analysis at the point-of-care (POC). The system utilizes a specially designed microfluidic disc for cell separation, solution metering and mixing, and cell counting, and is equipped with a custom script for automated quantification of cells. The proposed method shows a strong correlation with the gold standard hematology analyzer for various blood parameters. The portable system offers simplicity, affordability, and low power consumption, making it a potential solution for improving healthcare delivery in resource-limited settings and remote areas.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Nabil H. Bhuiyan, Joon S. Shim
Summary: Developing herd immunity is crucial for changing the course of the COVID-19 pandemic. An AI-driven point-of-care testing platform has been proposed for analyzing the body's response to SARS-CoV-2, and it has been successfully used for quantifying viral proteins and antibodies in blood samples. A data-receptive web-based dashboard system has also been developed for real-time analysis of herd immunity progress.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Alexiane Godain, Timothy M. Vogel, Pascal Fongarland, Naoufel Haddour
Summary: This study provides comprehensive insights into the intricate relationship between shear stress and electroactive biofilms in microbial fuel cells, highlighting the pivotal role of shear stress in influencing the growth kinetics, electrical performance, and physical structure of the biofilms. The study also emphasizes the complexities of extracellular electron transfer mechanisms and the need for complementary metaproteomic analyses.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Linjie Wang, Yixin Chen, Yang Ji, Shujun Zheng, Fei Wang, Caolong Li
Summary: A paper-based biosensor incorporating surface-enhanced Raman spectroscopy (SERS) and colorimetric detection has been developed for efficient quantification of intracellular glutathione (GSH). The biosensor exhibits excellent selectivity, stability, and precision, with low detection limits in both SERS and colorimetric modes. It has been successfully utilized for intracellular GSH detection and validated against a commercial GSH assay kit.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Quan Zhou, Jing Wang, Zhen Zhang, Alain Wuethrich, Richard J. Lobb, Matt Trau
Summary: This study presents a biosensor for sensitive detection of EMT-associated biomarkers on extracellular vesicles (EVs) surfaces during targeted therapy. Through longitudinal monitoring of patients, the biosensor shows its ability to identify the EMT-like phenotype switching on circulating EVs during the development of resistance.
BIOSENSORS & BIOELECTRONICS
(2024)
Review
Biophysics
Pratika Rai, Sabrina N. Hoba, Celine Buchmann, Robert J. Subirana-Slotos, Christian Kersten, Tanja Schirmeister, Kristina Endres, Bernd Bufe, Alexey Tarasov
Summary: Proteases have been proposed as potential biomarkers for various pathological conditions. The development of protease biosensors offers a more efficient way to investigate dysregulated proteases. This review article presents different optical and electrochemical detection methods for designing biosensors for all major protease families.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Chengxin Liu, Xu Shen, Li Yan, Runlian Qu, Yue Wang, Yaqin He, Zixuan Zhan, Piaopiao Chen, Feng Lin
Summary: In this study, a homogeneous rapid electrochemical aptasensor was developed to quantitatively detect CTCs in lung cancer patients. The aptasensor utilized a DNA nanosphere structure and a complementary aptamer to specifically detect mucin 1 as a marker for CTCs. The assay exhibited high specificity and sensitivity, and the results were consistent with other detection methods.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Danni Xue, Xing Dai, Jialong Zhao, Jiayao Zhang, Huan Liu, Kui Liu, Tao Xu, Chenjie Gu, Xingfei Zhou, Tao Jiang
Summary: In this study, a dual-enhancement SERS substrate based on Ti3C2TX and Ag nanocubes was fabricated for precise quantification of ritonavir and ibrutinib in serum. The formation of numerous electromagnetic hotspots between Ag nanocubes facilitated effective photo-induced charge transfer. The composite substrate showed excellent sensitivity, achieving low detection limits and high recoveries, making it promising for monitoring and identification of clinical blood drug concentration.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Tenghua Zhang, Zihui Xie, Xiaohe Zheng, Yuxin Liang, Yao Lu, Hankang Zhong, Feiyang Qian, Yuqing Zhu, Ruiting Sun, Yan Sheng, Jiaming Hu
Summary: This study reports a technology based on cationic lipid-polymer hybrid nanoparticles for efficient extracellular vesicle (EV) enrichment and in-situ detection of internal microRNAs. The technology demonstrates high EV enrichment efficiency and sensitive internal RNA detection, making it potentially useful for early pancreatic cancer diagnosis.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Wenjie Dai, Gaoxu Chen, Xiaoyan Wang, Shujun Zhen, Chengzhi Huang, Lei Zhan, Yuanfang Li
Summary: In this study, a novel metal organic gel (MOG) with dual electrochemiluminescence (ECL) properties was prepared by simple mixing. The MOG exhibited strong and stable anodic and cathodic ECL signals. By utilizing this MOG, an ECL resonance energy transfer (ECL-RET) biosensor was constructed for ultrasensitive detection of a specific gene. The study developed a straightforward technique for obtaining a single luminescent material with dual signals and expanded the analytical application of MOGs in the realm of ECL.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Lais Canniatti Brazaca, Amanda Hikari Imamura, Rodrigo Vieira Blasques, Jessica Rocha Camargo, Bruno Campos Janegitz, Emanuel Carrilho
Summary: The use of microfluidic paper-based analytical devices (muPADs) for medical diagnosis is a growing trend due to their low cost, easy use, simple manufacturing, and potential for application in low-resource settings. This review focuses on the advances in muPADs for medical diagnostics, discussing their use in detecting various biomarkers in common human biofluids. The challenges of biomarker detection in each sample are examined, along with innovative techniques to overcome these limitations. The commercialization difficulties of muPADs are also considered, along with future trends such as wearable devices and integrated platforms.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Minghong Jian, Xudong Sun, Hua Zhang, Xiaotong Li, Shasha Li, Zhenxin Wang
Summary: Matrix metalloproteinases (MMPs) are attractive biomarkers for cancer diagnosis and treatment, but their low abundance in biological samples, especially in the early stages of tumors, makes it challenging to precisely analyze MMP activities. In this study, a peptide microarray-based metal-enhanced fluorescence assay (PMMEFA) is proposed as a sensitive and specific method to simultaneously detect MMP-1, -2, -3, -7, -9, and -13 activities. The PMMEFA showed excellent sensitivity and was capable of detecting MMP activities in various matrices.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Kyung Ho Kim, Eunsu Ryu, Zinah Hilal Khaleel, Sung Eun Seo, Lina Kim, Yong Ho Kim, Hyun Gyu Park, Oh Seok Kwon
Summary: We have developed a novel strategy for discriminative detection of SARS-CoV-2 variants using the plasmonic photothermal effect of gold nanofilms and digital polymerase chain reaction (dPCR) technology. With this method, we were able to detect the delta and omicron variants with high sensitivity within 25 minutes from COVID-19 patients' clinical samples, making it a rapid and accurate point-of-care testing tool.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Zepeng Kang, Yuanming Wang, Haiyan Song, Xueli Wang, Job Zhang, Zhiguang Zhu
Summary: By designing a wearable and flexible lactic-acid/O2 EBFC with an air-breathing biocathode, the limitations of biocathode are effectively solved. The optimal performance conditions are determined through experiments, and the EBFC is successfully applied to power a low-power device.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Huayun Chen, Zhiheng You, Yuhui Hong, Xiao Wang, Mingming Zhao, Yushi Luan, Yibin Ying, Yixian Wang
Summary: This study developed a colorimetric sensor array using gas-responsive two-dimensional metal-organic framework (MOF) composites for highly sensitive detection of volatile organic compounds (VOCs). The dye/Zn-2(bim)(4) composites-based sensor arrays showed enhanced sensitivity and anti-interference properties. The findings demonstrate the potential use of dye/Zn-2(bim)(4) sensor arrays for early perception of plant diseases.
BIOSENSORS & BIOELECTRONICS
(2024)