Microscope images automatic focus algorithm based on eight-neighborhood operator and least square planar fitting

An automatic focus algorithm based on eight-neighborhood operator and least square planar fitting method is proposed to acquire the three-dimensional microscope focusing images in this paper. Firstly, eight-neighborhood operator and evaluation function are presented to locate the cells in the low-power microscope images in this paper. Then the automatic focus algorithm based on eight-neighborhood operator and least square planar fitting method is given to acquire the three-dimensional microscope cell images. After that, some experiments are given in the paper. The eight-neighborhood operator is compared with the traditional evaluation function in terms of the complexity of the operation and the effect of the evaluation function. Also, comparative experiments are conducted to verify the feasibility of the automatic focus algorithm in this paper. The experimental results showed that the proposed microscope automatic focus algorithm is effective and has a good fitting degree.