Self-powered competitive immunosensor driven by biofuel cell based on hollow-channel paper analytical devices

A mediator-less and compartment-less glucose/O-2 enzymatic biofuel cell (BFC) was introduced into microfluidic paper-based analytical devices (mu-PADs) that relies on flow in hollow channels with silver nanoparticles/graphene modified paper electrode as the anodic and cathodic substrate, to implement self-powered sensitive carcinoembryonic antigen (CEA) detection. Glucose dehydrogenase (GDH)-gold nanoparticles bioconjugate modified with CEA acted as a biocatalyst for enhancing glucose oxidation in the bioanode, as well as the transducing enzyme for signaling magnification. Similarly, nanoporous PtNi/bilirubin oxidase (BUD) acted as a biocatalyst for enhancing O-2 reduction in the biocathode. With an increase in the concentration of CEA, the amount of CEA-Au-GDH bioconjugate on bioanode decreases, thus leading to the lower output of the as-prepared SEC. This proposed BFC-based self-powered immunosensor for CEA possessed largely increased linear detection range from 1 pg mL(-1) to 0.5 mu g with a detection limit of 0.7 pg mL(-1). The proposed BFC-based self-powered immunosensor shows high sensitivity, stability, and reproducibility and can become a promising platform for other protein detection. (C) 2015 Elsevier B.V. All rights reserved.