Article
Biochemical Research Methods
Jongyun Kim, Arunkumar Shanmugasundaram, Cheong Bin Lee, Jae Rim Kim, Jeong Jae Park, Eung-Sam Kim, Bong-Kee Lee, Dong-Weon Lee
Summary: This study proposes a grooved polydimethylsiloxane (PDMS) membrane embedded with silver nanowires (AgNWs-E-PDMS) to enhance the maturation and contractile properties of cardiomyocytes and provide a more accurate evaluation of drug-induced cardiotoxicity.
Article
Biochemical Research Methods
Jongyun Kim, Arunkumar Shanmugasundaram, Cheong Bin Lee, Jae Rim Kim, Jeong Jae Park, Eung-Sam Kim, Bong-Kee Lee, Dong-Weon Lee
Summary: This study proposes a grooved polydimethylsiloxane (PDMS) membrane embedded with silver nanowires (AgNWs-E-PDMS) to enhance the maturation and contractile properties of cardiomyocytes. The proposed device provides a more accurate evaluation of drug-induced cardiotoxicity and demonstrates its feasibility as a drug screening platform.
Review
Biotechnology & Applied Microbiology
Kai Qiao, Lu Xu, Junnan Tang, Qiguang Wang, Khoon S. Lim, Gary Hooper, Tim B. F. Woodfield, Guozhen Liu, Kang Tian, Weiguo Zhang, Xiaolin Cui
Summary: With the aging and obesity of the population, an increasing number of patients are suffering from bone and cartilage injuries. Conventional therapies are unable to effectively stimulate cellular responses for cartilage regeneration, bone remodeling, and osseointegration. The rapid development of nanomedicine offers promising advancements in orthopedics, with enhanced drug loading efficiency, targeted capability, and improved therapeutic effects. This comprehensive review highlights the use of nanotechnology applications in the medical field, describes the development of nano delivery systems, and discusses the research advancements and future directions of nanomedicine for bone and cartilage repair.
JOURNAL OF NANOBIOTECHNOLOGY
(2022)
Review
Engineering, Biomedical
Dandan Zheng, Huitong Ruan, Wei Chen, Yuhui Zhang, Wenguo Cui, Hao Chen, Hongxing Shen
Summary: Extracellular vesicles (EVs) are nano-scale vesicles derived by cell secretion with unique advantages, which benefit their wide applications in regenerative medicine. However, the in vivo therapeutic effect of EVs still greatly restricted by several obstacles. This review summarizes different strategies to enhance EV functions and introduces the latest developments of functionalized EV-loaded biomaterials in different diseases. Challenges and future directions of EVs are also discussed.
BIOACTIVE MATERIALS
(2023)
Review
Biochemistry & Molecular Biology
Maryam Kay, Bahram M. Soltani
Summary: CM maturation is crucial for efficient and forceful contraction, and the molecular mechanisms, including the role of long noncoding RNAs (lncRNAs), are important in regulating various aspects of CM maturation. Understanding these mechanisms has implications for cell replacement therapy and regenerative medicine.
Review
Chemistry, Multidisciplinary
Yuwen Wang, Andy Tay
Summary: This review discusses the nano-scale properties of nanomaterials, focusing on nanoparticles, and their impact on nanomedicine. It explores the potential of manipulating the chirality of nanomaterials to enhance biomolecular interactions and improve nanotherapeutics. The review also examines the synthesis, characterization, and applications of chiral nanoparticles, particularly in cancer, immunomodulation, and neurodegenerative diseases, and proposes possible mechanisms for the interaction between chiral nanomaterials and cells for biological manipulation. This timely reminder emphasizes the importance of nanoscale modifications in advancing nanotherapeutics research.
Review
Biochemistry & Molecular Biology
Nikita Voloshin, Pyotr Tyurin-Kuzmin, Maxim Karagyaur, Zhanna Akopyan, Konstantin Kulebyakin
Summary: Immortalized cells are increasingly used in practical medicine due to their advantages, such as the ability to produce larger amounts of cells and use them for longer periods of time. However, they also have drawbacks, like the possibility of malignant transformation or major phenotype change. This review discusses ways to overcome these barriers and highlights specific fields of application where these concerns are minimal.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Engineering, Multidisciplinary
Hamed Nosrati, Masoud Nosrati
Summary: Regenerative medicine aims to repair, regenerate, or substitute impaired tissues and organs using cutting-edge approaches such as stem cell therapies and tissue engineering. Incorporating artificial intelligence (AI) technologies has opened new possibilities in this field, allowing for improved and accelerated research and development through the learning and application of data patterns.
Review
Biotechnology & Applied Microbiology
Liren Wang, Jia Jiang, Hai Lin, Tonghe Zhu, Jiangyu Cai, Wei Su, Jiebo Chen, Junjie Xu, Yamin Li, Jing Wang, Kai Zhang, Jinzhong Zhao
Summary: Regenerative sports medicine aims to treat sports and aging-related conditions using tissue regeneration techniques. This review summarizes the optimal regeneration techniques for each area and assesses the time required for feasible clinical application.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Review
Biotechnology & Applied Microbiology
Hao-Nan Wang, Xiao Rong, Lu-Ming Yang, Wei-Zhong Hua, Guo-Xin Ni
Summary: This review summarizes the advances of stem cells and stem cell-derived extracellular vesicles (EVs) in the repair of rotator cuff injuries, highlighting the underlying mechanisms and suggesting future directions for research.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Review
Chemistry, Multidisciplinary
Faranak Mankavi, Rana Ibrahim, Hongjun Wang
Summary: This article summarizes the recent advances in nerve guidance conduits (NGCs) for peripheral nerve regeneration and repair, including biomaterial innovations, structural design, advanced fabrication technologies, and cellular responses to biomimetic cues.
Review
Medicine, Research & Experimental
Vahid Mansouri, Nima Beheshtizadeh, Maliheh Gharibshahian, Leila Sabouri, Mohammad Varzandeh, Nima Rezaei
Summary: Regenerative medicine plays a crucial role in cancer treatment by providing novel therapies that alleviate patient suffering and accelerate healing. This field's development will continue to advance our understanding of cancer and promote future research in the field.
BIOMEDICINE & PHARMACOTHERAPY
(2021)
Review
Chemistry, Physical
Wei Zhu, Jinyi Zhang, Zhanqi Wei, Baozhong Zhang, Xisheng Weng
Summary: A hydrogel is a three-dimensional structure that can hold a large amount of water, but its brittleness limits its application. Self-healing hydrogels, a new type of hydrogel that can repair itself after external damage, have shown better fatigue resistance, reusability, hydrophilicity, and responsiveness to environmental stimuli. In the past decade, self-healing hydrogels have made rapid progress. They can automatically self-repair after external damage through strategies such as dynamic covalent bonds and reversible noncovalent interactions. Compared to traditional hydrogels, self-healing gels have improved durability, responsiveness, and plasticity, making them suitable for harsh environments and potential applications as drug carriers.
Review
Chemistry, Multidisciplinary
He Shen, Caixia Fan, Zhifeng You, Zhifeng Xiao, Yannan Zhao, Jianwu Dai
Summary: Spinal cord injury (SCI) leads to the loss of motor and sensory functions and understanding the pathophysiological changes and inhibitory microenvironment is crucial for potential mechanisms of functional restoration. Implantation of functionalized biomaterials and transplantation of spinal cord tissue grafts have shown promising results for SCI repair, opening up new avenues for treatment.
ADVANCED FUNCTIONAL MATERIALS
(2022)
Review
Polymer Science
Huaying Liu, Yuxuan Gong, Kaihui Zhang, Shen Ke, Yue Wang, Jing Wang, Haibin Wang
Summary: As an emerging 3D printing technology, 3D bioprinting is promising in tissue engineering and regenerative medicine. Decellularized extracellular matrices (dECM) are used to create unique tissue-specific bioink, and combining dECMs with 3D bioprinting can construct biomimetic hydrogels for bioinks. dECM has been proven as one of the fastest growing bioactive printing materials in cell-based 3D bioprinting.
Article
Biochemistry & Molecular Biology
Yaming Jiu, Reena Kumari, Aidan M. Fenix, Niccole Schaible, Xiaonan Liu, Markku Varjosalo, Ramaswamy Krishnan, Dylan T. Burnette, Pekka Lappalainen
Article
Biotechnology & Applied Microbiology
Kenneth R. Chien, Jonas Frisen, Regina Fritsche-Danielson, Douglas A. Melton, Charles E. Murry, Irving L. Weissman
NATURE BIOTECHNOLOGY
(2019)
Article
Cardiac & Cardiovascular Systems
Maria G. Lupo, Chiara Macchi, Silvia Marchiano, Riccardo Cristofani, Maria F. Greco, Stefano Dall'Acqua, Haixia Chen, Cesare R. Sirtori, Alberto Corsini, Massimiliano Ruscica, Nicola Ferri
NUTRITION METABOLISM AND CARDIOVASCULAR DISEASES
(2019)
Article
Cardiac & Cardiovascular Systems
Silvia Marchiano, Alessandro Bertero, Charles E. Murry
PEDIATRIC CARDIOLOGY
(2019)
Article
Biology
Kirsty Y. Wan, Sylvia K. Hurlimann, Aidan M. Fenix, Rebecca M. McGillivary, Tatyana Makushok, Evan Burns, Janet Y. Sheung, Wallace F. Marshall
PHILOSOPHICAL TRANSACTIONS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES
(2020)
Article
Cell & Tissue Engineering
Silvia Marchiano, Tien-Ying Hsiang, Akshita Khanna, Ty Higashi, Leanne S. Whitmore, Johannes Bargehr, Hongorzul Davaapil, Jean Chang, Elise Smith, Lay Ping Ong, Maria Colzani, Hans Reinecke, Xiulan Yang, Lil Pabon, Sanjay Sinha, Behzad Najafian, Nathan J. Sniadecki, Alessandro Bertero, Michael Gale Jr., Charles E. Murry
Summary: Research shows that SARS-CoV-2 selectively infects human pluripotent stem cell-derived cardiomyocytes, leading to a shift in host gene expression and impairment of cellular functions. This suggests that COVID-19-related cardiac symptoms may be a result of the direct cardiotoxic effect of the virus.
Article
Biochemistry & Molecular Biology
Maria Giovanna Lupo, Silvia Marchiano, Maria Pia Adorni, Francesca Zimetti, Massimiliano Ruscica, Maria Francesca Greco, Alberto Corsini, Nicola Ferri
Summary: Overexpression of PCSK9 in rat SMCs led to downregulation of LDL receptor and marker of contractile phenotype. This resulted in increased cell proliferation rate and partial resistance to simvastatin, indicating a potential role of PCSK9 in atherosclerotic plaque development and pharmacological response to statins.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Cell & Tissue Engineering
Kenta Nakamura, Lauren E. Neidig, Xiulan Yang, Gerhard J. Weber, Danny El-Nachef, Hiroshi Tsuchida, Sarah Dupras, Faith A. Kalucki, Anu Jayabalu, Akiko Futakuchi-Tsuchida, Daisy S. Nakamura, Silvia Marchiano, Alessandro Bertero, Melissa R. Robinson, Kevin Cain, Dale Whittington, Rong Tian, Hans Reinecke, Lil Pabon, Bjorn C. Knollmann, Steven Kattman, R. Scott Thies, W. Robb MacLellan, Charles E. Murry
Summary: Heart failure remains a significant cause of morbidity and mortality following myocardial infarction. Cardiac remuscularization with transplantation of human pluripotent stem cell-derived cardiomyocytes is a promising preclinical therapy to restore function. Recent large animal data, however, have revealed a significant risk of engraftment arrhythmia (EA). Pharmacologic treatment of EA may be a viable strategy to improve safety and allow further clinical development of cardiac remuscularization therapy.
Article
Medicine, Research & Experimental
Louisa Helms, Silvia Marchiano, Ian B. Stanaway, Tien-Ying Hsiang, Benjamin A. Juliar, Shally Saini, Yan Ting Zhao, Akshita Khanna, Rajasree Menon, Fadhl Alakwaa, Carmen Mikacenic, Eric D. Morrell, Mark M. Wurfel, Matthias Kretzler, Jennifer L. Harder, Charles E. Murry, Jonathan Himmelfarb, Hannele Ruohola-Baker, Pavan K. Bhatraju, Michael Gale, Benjamin S. Freedman
Summary: Kidneys are critical target organs of COVID-19, where SARS-CoV-2 specifically infects proximal tubules in organoids, leading to apoptosis and disrupted cell morphology. Gene expression patterns in organoids reflect proteomic signatures of COVID-19 in critically ill patients' urine, indicating upregulation of interferon pathway. Different SARS-CoV-2 viral variants show comparable infection levels in organoids, with infection ameliorated in ACE2(-/-) organoids and blocked by de novo-designed spike binder peptides.
Editorial Material
Cardiac & Cardiovascular Systems
Elaheh Karbassi, Charles E. Murry
Article
Biochemistry & Molecular Biology
Alexander M. M. Loiben, Wei-Ming Chien, Clayton E. E. Friedman, Leslie S. -L. Chao, Gerhard Weber, Alex Goldstein, Nathan J. J. Sniadecki, Charles E. E. Murry, Kai-Chun Yang
Summary: This study generated cardiomyocytes from induced pluripotent stem cells to model a pathogenic MYH7 missense variant associated with hypertrophic cardiomyopathy (HCM). The findings showed that this variant led to enlarged cardiomyocytes and reduced contractile function, as well as increased apoptosis. Interestingly, the apoptosis and contractile dysfunction were independent of p53 activity.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Engineering, Biomedical
Nicole Zeinstra, Ariana L. Frey, Zhiying Xie, Leslie P. Blakely, Ruikang K. Wang, Charles E. Murry, Ying Zheng
Summary: A modular fabrication approach using stacked patterned collagen membranes is reported to generate thick, large, three-dimensional, perfusable networks of endothelialized vasculature. The perfusable vascular networks show remodeling and evenly distributed perfusion in vitro while maintaining their patterned, open-lumen architecture. Implanted onto infarcted rat hearts, the perfusable vascular networks achieve greater host vascular integration and higher perfusion rate compared to self-assembled controls. This success represents an important step forward for vascular bioengineering and opens possibilities for more complex, large scale, highly metabolic engineered tissues.