Article
Plant Sciences
Hira Khan, Takashi Ochi
Summary: Non-homologous end joining (NHEJ) is important for repairing DNA double-strand breaks in plants, but the molecular mechanism of plant NHEJ is still unclear. This study discovered a previously unidentified plant ortholog of PAXX, which has a similar structure to human PAXX but functions similar to human XLF by interacting with Ku70/80 and XRCC4. This suggests that plant PAXX combines the roles of mammalian PAXX and XLF, indicating a functional redundancy between PAXX and XLF in plants.
Article
Oncology
Hao Hu, Wuqiang Chen, Shuo Zhang, Yuzheng Xue, Youzhao He, Yuanlong Gu
Summary: The study revealed that the upregulation of DNA-PKcs in PDAC cells is promoted by NEAT1 and miR-101, leading to the malignant behaviors of the cells. NEAT1 functions as an oncogene influencing cell proliferation, migration, and invasion.
Article
Biochemistry & Molecular Biology
Litao Wang, Lin Wu, Yuxin Du, Xiang Wang, Bingsheng Yang, Shuai Guo, Yuan Zhou, Yiming Xu, Shuofei Yang, Yingmei Zhang, Jun Ren
Summary: This study reveals the important role of DNA-PKcs in vascular remodeling, demonstrating its interaction with Drp1 and phosphorylation of Drp1S616 to promote mitochondrial fragmentation. Targeting DNA-PKcs may serve as a potential therapeutic option for the treatment of vascular remodeling and hypertension.
Review
Biochemistry & Molecular Biology
Ye -Rim Lee, Gi-Sue Kang, Taerim Oh, Hye-Ju Jo, Hye-Joon Park, G. -One Ahn
Summary: DNA-PKcs is a critical player in repairing DNA double-strand breaks and is involved in T and B cell development. Recent evidence suggests that DNA-PKcs not only functions in the nucleus but also in the cytoplasm, phosphorylating various proteins involved in cellular metabolism and cytokine production. Careful selection of experimental models is necessary when studying DNA-PKcs, as differences have been observed between cells with defective DNA-PKcs and cells with DNA-PKcs knockout. Additionally, the multiple functions and subcellular localization of DNA-PKcs in different cell types may complicate the effects of DNA-PK inhibitors in clinical trials.
MOLECULES AND CELLS
(2023)
Article
Respiratory System
Ying-Ying Liu, Wei-Yun Zhang, Meng-Lan Zhang, Yu-Ji Wang, Xi-Yan Ma, Jung-Hong Jiang, Ran Wang, Da-Xiong Zeng
Summary: This study demonstrated the expression and potential mechanism of DNA-PKcs in the development of hypoxic pulmonary hypertension. The findings suggest that DNA-PKcs could be a potential therapeutic target for pulmonary vascular remodeling and pulmonary hypertension.
RESPIRATORY RESEARCH
(2022)
Article
Biochemistry & Molecular Biology
Waldemar Wagner, Katarzyna Sobierajska, Katarzyna Dominika Kania, Edyta Paradowska, Wojciech Michal Ciszewski
Summary: The study reveals that lactate can decrease lentiviral transduction efficiency by increasing the nuclear localization of DNA-PKcs, and the stimulation of cells with HCA1 agonist or HDAC inhibitor can mimic this effect. Additionally, inhibiting lactate flux can also reduce the nuclear localization of DNA-PKcs, leading to diminished lentiviral transduction efficacy.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Tizia Matthaeus, Sandra Stoesser, Hatice Yasemin Seren, Vivien M. M. Haberland, Andrea Hartwig
Summary: In this study, the negative effect of arsenite on BRCA1-mediated DNA damage response mechanisms was investigated. Arsenite treatment impaired the function of BRCA1, leading to a shift from error-free homologous recombination (HR) to error-prone non-homologous end-joining (NHEJ), which may compromise genomic stability.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Aashish Soni, Xiaolu Duan, Martin Stuschke, George Iliakis
Summary: The activation of the intra-S-phase checkpoint is essential for maintaining genomic stability and involves the activities of ATM and ATR. DNA-PKcs also contributes to the recovery from the checkpoint. The organization of the intra-S-phase checkpoint is similar to that of the G(2) checkpoint.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Biochemistry & Molecular Biology
Lisa Lirussi, Ozlem Demir, Panpan You, Antonio Sarno, Rommie E. Amaro, Hilde Nilsen
Summary: RNA modifications play vital roles in proper RNA processing and maturation, with enzymes like SMUG1 able to recognize and process modified RNA substrates. SMUG1 interacts with dyskerin to regulate rRNA metabolism, impacting post-transcriptional regulation and quality control of rRNAs. Cells lacking SMUG1 show elevated levels of immature rRNA molecules and accumulation of 5hmU in mature rRNA, suggesting a crucial role for SMUG1 in RNA surveillance.
Article
Cell Biology
Bernhard N. Bohnert, Irene Gonzalez-Menendez, Thomas Doerffel, Jonas C. Schneider, Mengyun Xiao, Andrea Janessa, M. Zaher Kalo, Birgit Fehrenbacher, Martin Schaller, Nicolas Casadei, Kerstin Amann, Christoph Daniel, Andreas L. Birkenfeld, Florian Grahammer, Lahoucine Izem, Edward F. Plow, Leticia Quintanilla-Martinez, Ferruh Artunc
Summary: In susceptibility to doxorubicin-induced nephropathy, the interaction between the C6418T SNP in the Prkdc gene and Plg plays a crucial role, following a hierarchical two-hit process in 129S1/SvImJ mice.
DISEASE MODELS & MECHANISMS
(2021)
Review
Biochemistry & Molecular Biology
Go Watanabe, Michael R. Lieber
Summary: Nonhomologous DNA end joining is the major pathway for repairing double-strand breaks in cells, and Artemis serves as the major nuclease in this process. The activity of Artemis is tightly regulated by the DNA-PKcs kinase, which interacts with Artemis and is activated by broken DNA ends. Studying the structure of the Artemis:DNA-PKcs complex and its relevance to biochemistry and the immune system provides important insights into the cellular DNA repair process.
JOURNAL OF MOLECULAR BIOLOGY
(2022)
Review
Medicine, General & Internal
Eihab A. Subahi, Fateen Ata, Hassan Choudry, Phool Iqbal, Mousa A. AlHiyari, Ashraf T. Soliman, Vincenzo De Sanctis, Mohamed A. Yassin
Summary: This article is a systematic review of the occurrence of extramedullary haematopoiesis (EMH) in patients with beta-thalassaemia major (BTM). The results show that EMH is rare in BTM patients and can occur in any organ system with varied clinical features. Magnetic resonance imaging (MRI) is an effective diagnostic modality for EMH, and conservative management has similar outcomes compared to invasive treatments. Larger studies focusing on outcomes are needed to improve guidelines for managing EMH in BTM.
ANNALS OF MEDICINE
(2022)
Article
Cell Biology
Theresa Farhat, Amel Dudakovic, Jay H. Chung, Andre J. van Wijnen, Rene St-Arnaud
Summary: The catalytic subunit of DNA-PKcs plays a negative regulatory role in osteoblast differentiation, and inhibitors of DNA-PKcs may have therapeutic potential for bone regeneration and metabolic bone diseases.
JOURNAL OF CELLULAR PHYSIOLOGY
(2021)
Article
Ecology
Daniel Fonseca Teixeira, Heron Oliveira Hilario, Gilmar Bastos Santos, Daniel Cardoso Carvalho
Summary: The study of ichthyoplankton is crucial for understanding fish reproduction dynamics, and DNA metabarcoding is an effective tool for identifying multiple individuals simultaneously. However, there are challenges associated with DNA metabarcoding, including choosing the appropriate molecular markers, primer bias, and incomplete reference databases.
ECOLOGY AND EVOLUTION
(2023)
Article
Plant Sciences
Monika Zakrzewska-Placzek, Anna Golisz-Mocydlarz, Michal Krzyszton, Justyna Piotrowska, Malgorzata Lichocka, Joanna Kufel
Summary: This study demonstrates the involvement of AtNOL12 in rRNA biogenesis and its correlation with stress response in plants. The absence of AtNOL12 alters the expression of ribosomal protein and ribosome biogenesis genes, potentially impacting ribosome function.
Article
Gastroenterology & Hepatology
Ane Olazagoitia-Garmendia, Linda Zhang, Paula Mera, Julie K. Godbout, Maialen Sebastian-DelaCruz, Iraia Garcia-Santisteban, Luis Manuel Mendoza, Alain Huerta, Inaki Irastorza, Govind Bhagat, Peter H. Green, Laura Herrero, Dolors Serra, Jose Antonio Rodriguez, Elena F. Verdu, Chuan He, Jose Ramon Bilbao, Ainara Castellanos-Rubio
Summary: This study identifies a novel m(6)A-XPO1-NFkB pathway that is activated in CD patients, prompting the development of new therapeutic approaches directed at m(6)A proteins and XPO1, a target under evaluation for the treatment of intestinal disorders. The functional involvement of a CD-associated SNP located in the 5'UTR of XPO1 is described, shedding light on the role of this SNP in triggering inflammation in coeliac intestinal epithelium.
Article
Hematology
Ferdinando Bonfiglio, Alessio Bruscaggin, Francesca Guidetti, Lodovico Terzi di Bergamo, Martin Faderl, Valeria Spina, Adalgisa Condoluci, Luisella Bonomini, Gabriela Forestieri, Ricardo Koch, Deborah Piffaretti, Katia Pini, Maria Cristina Pirosa, Micol Giulia Cittone, Alberto Arribas, Marco Lucioni, Guido Ghilardi, Wei Wu, Luca Arcaini, Maria Joao Baptista, Gabriela Bastidas, Silvia Bea, Renzo Boldorini, Alessandro Broccoli, Marco Matteo Buehler, Vincenzo Canzonieri, Luciano Cascione, Luca Ceriani, Sergio Cogliatti, Paolo Corradini, Enrico Derenzini, Liliana Devizzi, Sascha Dietrich, Angela Rita Elia, Fabio Facchetti, Gianluca Gaidano, Juan Fernando Garcia, Bernhard Gerber, Paolo Ghia, Maria Gomes da Silva, Giuseppe Gritti, Anna Guidetti, Felicitas Hitz, Giorgio Inghirami, Marco Ladetto, Armando Lopez-Guillermo, Elisa Lucchini, Antonino Maiorana, Roberto Marasca, Estella Matutes, Veronique Meignin, Michele Merli, Alden Moccia, Manuela Mollejo, Carlos Montalban, Urban Novak, David Graham Oscier, Francesco Passamonti, Francesco Piazza, Stefano Pizzolitto, Alessandro Rambaldi, Elena Sabattini, Gilles Salles, Elisa Santambrogio, Lydia Scarfo, Anastasios Stathis, Georg Stussi, Julia T. Geyer, Gustavo Tapia, Corrado Tarella, Catherine Thieblemont, Thomas Tousseyn, Alessandra Tucci, Giorgio Vanini, Carlo Visco, Umberto Vitolo, Renata Walewska, Francesco Zaja, Thorsten Zenz, Pier Luigi Zinzani, Hossein Khiabanian, Arianna Calcinotto, Francesco Bertoni, Govind Bhagat, Elias Campo, Laurence De Leval, Stefan Dirnhofer, Stefano A. Pileri, Miguel A. Piris, Alexandra Traverse-Glehen, Alexander Tzankov, Marco Paulli, Maurilio Ponzoni, Luca Mazzucchelli, Franco Cavalli, Emanuele Zucca, Davide Rossi
Summary: Splenic marginal zone B-cell lymphoma (SMZL) is a heterogeneous clinico-biological entity with different subgroups characterized by genetic abnormalities, pathway signatures, and microenvironment compositions. These findings provide insights into the classification and treatment of SMZL.
Editorial Material
Cell Biology
Demis Menol, Shan Zha
Summary: Recent structural studies have revealed the molecular details of the DNA-PKcs kinase's crucial role in the non-homologous end-joining DNA-double strand break repair pathway. These studies have highlighted how the kinase protects DNA ends, recruits and activates endonucleases for end-processing, and phosphorylates itself for end-ligation.
TRENDS IN CELL BIOLOGY
(2022)
Article
Cell & Tissue Engineering
Shawn M. Sarkaria, Junsong Zhou, Suying Bao, Wenqi Zhao, Yinshan Fang, Jianwen Que, Govind Bhagat, Chaolin Zhang, Lei Ding
Summary: Remodeling of the bone marrow microenvironment is observed in primary myelofibrosis (PMF), characterized by expansion of collagen-expressing myofibroblasts derived from LepR+ mesenchymal cells and upregulation of arteriolar-signature genes in endothelial cells. Unbiased single-cell RNA sequencing confirmed the origin of myofibroblasts and revealed reduced expression of hematopoietic niche factors and increased expression of fibrogenic factors. Expansion of pericytes and Sox10+ glial cells and enhanced cell-cell signaling suggest their functional roles in PMF. Targeting bone marrow glial cells could ameliorate fibrosis and improve pathology in PMF.
Article
Computer Science, Interdisciplinary Applications
Oliver Faust, Simona De Michele, Joel E. W. Koh, V Jahmunah, Oh Shu Lih, Aditya P. Kamath, Prabal Datta Barua, Edward J. Ciaccio, Suzanne K. Lewis, Peter H. Green, Govind Bhagat, U. Rajendra Acharya
Summary: This study aimed to utilize AI models to distinguish between normal individuals, individuals with celiac disease (CD), and individuals with non-celiac duodenitis (NCD) based on characteristics of the small intestinal lamina propria. By preprocessing high magnification biopsy images, the study found that AI models can effectively differentiate between different types of intestinal disorders and can potentially be applied in clinical settings.
COMPUTER METHODS AND PROGRAMS IN BIOMEDICINE
(2023)
Letter
Oncology
Craig R. Soderquist, Susan Hsiao, Jiuhong Pang, Alejandro A. Gru, Sameera Husain, Bachir Alobeid, Govind Bhagat
HEMATOLOGICAL ONCOLOGY
(2023)
Article
Multidisciplinary Sciences
Sofija Vlasevska, Laura Garcia-Ibanez, Romain Duval, Antony B. Holmes, Rahat Jahan, Bowen Cai, Andrew Kim, Tongwei Mo, Katia Basso, Rajesh K. Soni, Govind Bhagat, Riccardo Dalla-Favera, Laura Pasqualucci
Summary: Heterozygous inactivating mutations of KMT2D and CREBBP are common in B cell lymphoma, and they co-occur in a significant number of follicular lymphoma and EZB/C3 diffuse large B cell lymphoma cases. The combination of haploinsufficiency of Crebbp and Kmt2d promotes the expansion of abnormally polarized germinal centers, which is an early event in lymphoma development. These findings highlight the functional and biochemical interaction between Crebbp and Kmt2d, and its implications for the treatment of lymphomas with enhancer defects induced by their combined loss.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Article
Multidisciplinary Sciences
Yimeng Zhu, Wenxia Jiang, Brian J. Lee, Angelina Li, Steven Gershik, Shan Zha, Sue Jinks-Robertson
Summary: The nonhomologous end-joining (NHEJ) pathway is crucial for DNA double-strand break repair and lymphocyte development. The Ku70 and Ku80 heterodimer initiates NHEJ by recruiting DNA-dependent protein kinase (DNA-PKcs). Phosphorylation of DNA-PKcs at S2056 is important for end-ligation, but its physiological significance is still unclear. Xlf deficiency leads to defective lymphocyte development, which can be restored by DNA-PKcs phosphorylation. These findings highlight the role of DNA-PKcs S2056 cluster phosphorylation in physiological chromosomal NHEJ and its synergy with XLF.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Article
Multidisciplinary Sciences
Demis Menolfi, Brian J. Lee, Hanwen Zhang, Wenxia Jiang, Nicole E. Bowen, Yunyue Wang, Junfei Zhao, Antony Holmes, Steven Gershik, Raul Rabadan, Baek Kim, Shan Zha
Summary: The ATR kinase is crucial for the replication stress response and also supports normal cell proliferation by regulating origin firing to prevent depletion of dNTPs and other replication factors.
NATURE COMMUNICATIONS
(2023)
Article
Biochemistry & Molecular Biology
Iyshwarya Balasubramanian, Sheila Bandyopadhyay, Juan Flores, Jared Bianchi-Smak, Xiang Lin, Haoran Liu, Shengxiang Sun, Natasha B. Golovchenko, Yue Liu, Dahui Wang, Radha Patel, Ivor Joseph, Panan Suntornsaratoon, Justin Vargas, Peter H. R. Green, Govind Bhagat, Stephen M. Lagana, Wang Ying, Yi Zhang, Zhihan Wang, Wei Vivian Li, Sukhwinder Singh, Zhongren Zhou, George Kollias, Laura A. Farr, Shannon N. Moonah, Shiyan Yu, Zhi Wei, Edward M. Bonder, Lanjing Zhang, Pawel R. Kiela, Karen L. Edelblum, Ronaldo Ferraris, Ta-Chiang Liu, Nan Gao
Summary: Paneth cells play a crucial role in host responses to microbiome and environmental stresses. Infection can modify the composition of Paneth cells and affect inflammation and disease progression.
Correction
Oncology
Rita Alaggio, Catalina Amador, Ioannis Anagnostopoulos, Ayoma D. Attygalle, Iguaracyra Barreto de Oliveira Araujo, Emilio Berti, Govind Bhagat, Anita Maria Borges, Daniel Boyer, Mariarita Calaminici, Amy Chadburn, John K. C. Chan, Wah Cheuk, Wee-Joo Chng, John K. Choi, Shih-Sung Chuang, Sarah E. Coupland, Magdalena Czader, Sandeep S. Dave, Daphne de Jong, Arianna Di Napoli, Ming-Qing Du, Kojo S. Elenitoba-Johnson, Judith Ferry, Julia Geyer, Dita Gratzinger, Joan Guitart, Sumeet Gujral, Marian Harris, Christine J. Harrison, Sylvia Hartmann, Andreas Hochhaus, Patty M. Jansen, Kennosuke Karube, Werner Kempf, Joseph Khoury, Hiroshi Kimura, Wolfram Klapper, Alexandra E. Kovach, Shaji Kumar, Alexander J. Lazar, Stefano Lazzi, Lorenzo Leoncini, Nelson Leung, Vasiliki Leventaki, Xiao-Qiu Li, Megan S. Lim, Wei-Ping Liu, Abner Louissaint, Andrea Marcogliese, L. Jeffrey Medeiros, Michael Michal, Roberto N. Miranda, Christina Mitteldorf, Santiago Montes-Moreno, William Morice, Valentina Nardi, Kikkeri N. Naresh, Yasodha Natkunam, Siok-Bian Ng, Ilske Oschlies, German Ott, Marie Parrens, Melissa Pulitzer, S. Vincent Rajkumar, Andrew C. Rawstron, Karen Rech, Andreas Rosenwald, Jonathan Said, Clementine Sarkozy, Shahin Sayed, Caner Saygin, Anna Schuh, William Sewell, Reiner Siebert, Aliyah R. Sohani, Ritsuro Suzuki, Reuben Tooze, Alexandra Traverse-Glehen, Francisco Vega, Beatrice Vergier, Ashutosh D. Wechalekar, Brent Wood, Luc Xerri, Wenbin Xiao, Norah Olubunmi Akinola, Yassmine Akkari, Luis M. Allende, Katsuyuki Aozasa, Iguaracyra Araujo, Luca Arcaini, Kirit M. Ardeshna, Naoko Asano, Andishe Attarbaschi, Chris M. Bacon, Sharon Louise Barrans, Tracy Batchelor, Maxime Battistella, Linda B. Baughn, Amir Behdad, Sigbjorn Berentsen, Giada Bianchi, Jacob Bledsoe, Peter Borchmann, Mark Bower, Barbara Buldini, Jan Andreas Burger, Birgit Burkhardt, Ryan D. Cassaday, Giovanni Cazzaniga, Nadine Cerf-Bensussan, Ethel Cesarman, Mammen Chandy, Jennifer R. Chapman, Bjorn Chapuy, Xueyan Chen, Chee Leong Cheng, Carlos Chiattone, Nicholas Chiorazzi, Lucy B. Cook, Wendy A. Cooper, Gregory Philip Corboy, Andrew John Cowan, Immacolata Cozzolino, Ian A. Cree, Emanuele S. G. d'Amore, Andrew John Davies, Martina Deckert, Jan Delabie, Elizabeth G. Demicco, Vikram Deshpande, Arjan Diepstra, Daan Dierickx, Kieron Dunleavy, Barbara Eichhorst, Daisuke Ennishi, David C. Fajgenbaum, Pedro Farinha, Carlos Fernandez de Larrea, Kevin E. Fisher, Jude Fitzgibbon, Melina Flanagan, Jonathan Fromm, Juan F. Garcia, William Robert Geddie, Morie Gertz, Ajay Gopal, Satish Gopal, Patricia Theresa Greipp, Alejandro Gru, Ritu Gupta, Martin-Leo Hansmann, Konnie M. Hebeda, Klaus Herfarth, Marco Herling, Olivier Hermine, Khe Hoang-Xuan, Jennelle Hodge, Shimin Hu, Yuhua Huang, Yin Pun Hung, Stephen Hunger, Hiroto Inaba, Hiroshi Inagaki, Javeed Iqbal, Kenji Ishitsuka, Noriko Iwaki, Keiji Iwatsuki, Nitin Jain, Yoon Kyung Jeon, Marshall Kadin, Sachiko Kaji, Aanchal Kakkar, Anastasios Karadimitris, Keisuke Kataoka, Seiichi Kato, Marie Jose Kersten, Rhett P. Ketterling, Ji Eun Kim, Christian P. Kratz, Robert Kridel, Sigurdur Kristinsson, Ralf Kuppers, Isinsu Kuzu, Yok-Lam, Ann Lacasce, Laurence Lamant-Rochaix, Thierry Lamy, Ola Landgren, Siddhartha Laskar, William Bradlyn Laskin, Georg Lenz, Shaoying Li, Gan Di Li, Pei Lin, Franco Locatelli, Robert Brian Lorsbach, Izidore Lossos, Thomas P. Jr Loughran, William R. Macon, Joseph J. Maleszewski, Pankaj Malhotra, Teresa Marafioti, Dai Maruyama, Alexander Marx, Sam M. Mbulaiteye, Veronique Meignin, Ester Mejstrikova, Pamela Michelow, Markku Miettinen, Rodney R. Miles, Hiroaki Miyoshi, Thierry Jo Molina, Manuela Mollejo, Shuji Momose, Tetsuya Mori, William G. Morice, Bertrand Nadel, Hirokazu Nagai, Motoo Nagane, Reena Nair, Naoya Nakamura, Atsuko Nakazawa, Samih Nasr, Andrew Gordon Nicholson, Alina Nicolae, Robert Shigeo Ohgami, Naoki Oishi, Timothy S. Olson, Nicolas Ortonne, Bruno Paiva, Qiang Pan-Hammarstrom, Mayur Parihar, Marco Paulli, Andrea Petersen, Jennifer Picarsic, Alessandro Pileri, Nicola Pimpinelli, Jose A. Plaza, Karen R. Rabin, Markus Raderer, Kanti Rai, Ulla Randen, Huilan Rao, Alistair Robson, Rosemary Rochford, Richard Rosenquist, Davide Rossi, Esther D. Rossi, Simon Rule, Grzegorz Rymkiewicz, Elena Sabattini, Vaskar Saha, Mamiko Sakata-Yanagimoto, Christian A. Sander, J. Martin Sangueza, Omar P. Sangueza, Marco Santucci, Yasuharu Sato, Akira Satou, Kristian Theo Schafernak, Fernando Schmitt, Gianpietro Semenzato, Manju Sengar, Tait Shanafelt, Kazuyuki Shimada, Graham W. Slack, Susan Slager, Riccardo Soffietti, David A. Solomon, Kostas Stamatopoulos, Christian Steidl, Stephan Stilgenbauer, Narittee Sukswai, Kengo Takeuchi, Giovanni Tallini, Junichi Tamaru, Soo-Yong Tan, Prashant Tembhare, Enrico Tiacci, Yoshiki Tokura, Olivier Tournilhac, Steven Treon, Lorenz Truemper, Kunihiro Tsukasaki, Frits van Rhee, Abraham Varghese, Maarten H. Vermeer, Philippe Vielh, Brian Walker, Michael Wang, Huan-You Wang, Zhe Wang, Takashi Watanabe, Oliver Weigert, David Wein-Stock, Sean J. Whittaker, Rein Willemze, Wilhelm Woessmann, Catherine J. Wu, Motoko Yamaguchi, Hidetaka Yamamoto, Daisuke Yamashita, Shenmiao Yang, David T. Yang, Takahiko Yasuda, Wei-Hua Yin, Yoh Zen, Sha Zhao, Wei-Li Zhao
Article
Cell Biology
Nima Jaberi-Lashkari, Byron Lee, Fardin Aryan, Eliezer Calo
Summary: This study reveals that the structure and evolution of a recently emerged biomolecular condensate, the nucleolar fibrillar center (FC), is determined by a single self-assembling protein TCOF1. TCOF1 forms the FC through self-assembly mediated by homotypic interactions of serine/glutamate-rich low-complexity regions (LCRs). Furthermore, introducing TCOF1 into a species lacking the FC is sufficient to form an FC-like biomolecular condensate.
Article
Immunology
Adam Kornberg, Theo Botella, Christine S. S. Moon, Samhita Rao, Jared Gelbs, Liang Cheng, Jonathan Miller, Alyssa M. M. Bacarella, Javier A. A. Garcia-Vilas, Justin Vargas, Xuechen Yu, Izabela Krupska, Erin Bush, Reuben Garcia-Carrasquillo, Benjamin Lebwohl, Suneeta Krishnareddy, Suzanne Lewis, Peter H. R. Green, Govind Bhagat, Kelley S. S. Yan, Arnold Han
Summary: Celiac disease (CD) is an autoimmune disease characterized by intestinal inflammation triggered by dietary gluten. This study conducted single-cell analysis of T cells in the intestine and peripheral blood of CD patients in different disease states. The results showed that CD was associated with activated intestinal T cells, including follicular T helper cells, regulatory T cells, and natural killer cell receptor-expressing T cells. After gluten ingestion, cytotoxic T cells in CD were mobilized along with gluten-specific CD4(+) T cells. These findings suggest a rapid response of cytotoxic T cells in CD after gluten exposure.
SCIENCE IMMUNOLOGY
(2023)
Meeting Abstract
Medicine, Research & Experimental
Veronica Russell, Sheren Younes, C. Cameron Yin, Shaoying Li, Jie Xu, Govind Bhagat, Yuri Fedoriw, Amy Chadburn, Jennifer Chapman, Izidore Lossos, Michele Stanchina, Elizabeth Thacker, Tushar Dave, Jean Koff, Christine Goss, Mary Ann Arildsen, Ridas Juskevicius, Sarah Ondrejka, Eric Hsi, David Jaye, Yasodha Natkunam, Sandeep Dave
LABORATORY INVESTIGATION
(2023)
Article
Oncology
Kenneth Ofori, Diane Chen, Jorge Sepulveda, Govind Bhagat, Bachir Alobeid
Summary: This study found that normoblastemia is present in approximately 28.6% of COVID-19 patients. This abnormality is associated with markers of severe disease, extramedullary erythropoiesis, and adverse clinical outcomes.
INTERNATIONAL JOURNAL OF CLINICAL AND EXPERIMENTAL PATHOLOGY
(2023)