期刊
NANOMEDICINE
卷 15, 期 11, 页码 1079-1096出版社
FUTURE MEDICINE LTD
DOI: 10.2217/nnm-2019-0417
关键词
apoptosis; autophagy; cytotoxicity; gold nanoparticles; renal carcinoma cells; renal cells
资金
- National Natural Science Foundation of China [81771968, 21704061, 81773222]
- Experimental Animal Project from Science and Technology Commission of Shanghai [15140902700]
- Medical-Engineering Joint Funds from the Shanghai Jiao Tong University [ZH2018ZDA01, YG2011MS53]
- Fund of Shanghai Jiao Tong University School of Medicine [12XJ10079]
- Shanghai Municipal Education Commission - Gaofeng Clinical Medicine Grant Support [20181705]
Aim: To research the influence and mechanism of gold nanoparticles (AuNPs) with different size for HK-2 cells (kidney normal cells) and 786-0 cells (kidney cancer cells). Materials & methods: HK-2 cells and 786-0 cells were treated with 5 and 200 nm AuNPs at 1 and 10 mu g/ml. The cell viability, intracellular reactive oxygen species levels, cell apoptosis, cell autophagy, and related cell signaling pathways were analyzed. Results: In HK-2 cells, AuNPs reduced the activity of Akt and mTOR and upregulated the expression of LC3 II. In 786-0 cells, the activity of p38 was upregulated, which leaded to the increase of caspase 3 and initiated apoptosis. Conclusion: AuNPs of 5 and 200 nm at 10 mu g/ml exerted antitumor effect by prompting apoptosis and inhibiting proliferation, while autophagy was activated to protect HK-2 cells from AuNPs-induced cytotoxicity.
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