Article
Plant Sciences
Zuntao Xu, Enze Li, Gan Xue, Cheng Zhang, Yachun Yang, Yong Ding
Summary: This study reveals that OsHUB2 represses the function of OsTrx1 and H3K4me3 levels at Ehd1, and suggests that OsHUB2-mediated H2B ubiquitination plays critical roles together with H3K4me3 in rice heading date regulation.
Article
Biochemistry & Molecular Biology
Rachel Bayley, Valerie Borel, Rhiannon J. Moss, Ellie Sweatman, Philip Ruis, Alice Ormrod, Amalia Goula, Rachel M. A. Mottram, Tyler Stanage, Graeme Hewitt, Marco Saponaro, Grant S. Stewart, Simon J. Boulton, Martin R. Higgs
Summary: The study suggests that methylation of histone H3 by SETD1A-BOD1L contributes to the recruitment of RIF1 to double-strand breaks, preventing excessive degradation of DNA ends and maintaining genome stability. This mechanism plays a crucial role in preserving telomere function and class switch recombination, and may be associated with the treatment and resistance of certain cancers.
Article
Biochemistry & Molecular Biology
Zhiheng Deng, Huasong Ai, Maoshen Sun, Zebin Tong, Yunxiang Du, Qian Qu, Liying Zhang, Ziyu Xu, Shixian Tao, Qiang Shi, Jia-Bin Li, Man Pan, Lei Liu
Summary: This study elucidates the mechanism of H2B monoubiquitylation and highlights the critical role of nucleosomal DNA in mediating E3 ligase recognition.
Article
Biochemistry & Molecular Biology
Robyn Laura Kosinsky, Maria Zerche, Ana Patricia Kutschat, Asha Nair, Zhenqing Ye, Dominik Saul, Maximilian von Heesen, Jessica J. Friton, Ana Carolina Schwarzer, Nadia Paglilla, Shehzad Z. Sheikh, Florian Wegwitz, Zhifu Sun, Michael Ghadimi, Rodney D. Newberry, R. Balfour Sartor, William A. Faubion, Steven A. Johnsen
Summary: Despite the identification of genetic factors linked to IBD susceptibility, the mechanisms behind it are still unclear. The RNF20/RNF40/H2Bub1 axis plays a critical role in promoting intestinal inflammation in IBD patients and mouse models, revealing their importance as regulators of IBD.
CELL DEATH AND DIFFERENTIATION
(2021)
Article
Microbiology
Ruoyan Liu, Xiaoyu Chen, Fujie Zhao, Yixuan Jiang, Zhenguo Lu, Huining Ji, Yuanyuan Feng, Junqiang Li, Heng Zhang, Jianting Zheng, Jing Zhang, Youbao Zhao
Summary: The COMPASS protein complex plays a crucial role in regulating gene expression and virulence in Cryptococcus neoformans. The core subunits Set1, Bre2, Swd1, and Swd3 act as a catalytic core and control the development and virulence of the fungus.
Article
Cell Biology
Shinya Takahata, Saori Chida, Aoi Ohnuma, Motoyoshi Ando, Takahiro Asanuma, Yota Murakami
Summary: In heterochromatin, Spt16 recruitment is mediated by Pob3 and HP1/Swi6. Pob3 recruits Spt16 through its Spt16 dimerization and PH domains, while HP1/Swi6 recruits Spt16 through physical interaction of the Swi6 CSD and Spt16 peptidase-like domains.
Article
Biochemistry & Molecular Biology
Guangxin Zhao, Jingying Wang, Xi Chen, Hanjing Sha, Xin Liu, Yunfei Han, Guankai Qiu, Fantao Zhang, Jun Fang
Summary: Here, we demonstrate the important role of OsASHL1 and OsASHL2 in rice growth and development, regulating floral transition and plant hormone metabolism.
JOURNAL OF GENETICS AND GENOMICS
(2022)
Article
Multidisciplinary Sciences
Marco Igor Valencia-Sanchez, Pablo De Ioannes, Miao Wang, David M. Truong, Rachel Lee, Jean-Paul Armache, Jef D. Boeke, Karim-Jean Armache
Summary: This study demonstrates that acetylation of histone H4 can stimulate the activity of yeast Dot1 in a specific manner, which coordinates with histone H2B ubiquitination to affect H3K79 methylation. H4K16ac and H2BUb play crucial roles in histone cross-talk, regulating gene transcription and gene silencing together.
Article
Multidisciplinary Sciences
Nathan J. VanDusen, Julianna Y. Lee, Weiliang Gu, Catalina E. Butler, Isha Sethi, Yanjiang Zheng, Justin S. King, Pingzhu Zhou, Shengbao Suo, Yuxuan Guo, Qing Ma, Guo-Cheng Yuan, William William Pu
Summary: The study utilized in vivo somatic Cas9 mutagenesis in mice to conduct a forward genetic screen for regulators of cardiomyocyte maturation, identifying transcriptional regulators such as RNF20 and RNF40. Mechanistic studies showed that these regulators control dynamic gene expression changes essential for cardiomyocyte maturation.
NATURE COMMUNICATIONS
(2021)
Article
Oncology
Dev Karan, Manohar Singh, Seema Dubey, Peter J. Van Veldhuizen, Yogen Saunthararajah
Summary: In this study, the unique role of guadecitabine (gDEC) in activating lysine methyl-transferases (KMTs) and its association with prostate cancer inhibition was described. It was found that gDEC upregulated epigenetic activating enzymes and contributed to the overall change in prostate cancer phenotype and anticancer effect independent of apoptosis. Mechanistically, gDEC increased the methylation of H3K4 in prostate cancer cell lines by depleting DNMT1. This study highlights the therapeutic potential of gDEC in reversing aberrant epigenetic repression and treating prostate cancer.
Article
Oncology
Lucile M. Jeusset, Kirk J. McManus
Summary: Monoubiquitination of histone H2B on lysine 120 plays a crucial role in various biological processes. Aberrant regulation of H2Bub1 can lead to transcriptional reprogramming and genome instability, thereby promoting oncogenesis. Understanding the misregulation of H2Bub1 may reveal novel drug targets and therapeutic strategies for cancer treatment.
SEMINARS IN CANCER BIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
James K. Fields, Chad W. Hicks, Cynthia Wolberger
Summary: Post-translational modification of histones, including methylation and monoubiquitination, plays a crucial role in transcription regulation. Methylation of H3K4 and H3K79 activates transcription, while methylation of H3K27 represses transcription. These modifications are dependent on prior monoubiquitination of specific histone residues, leading to a phenomenon called histone crosstalk. Recent studies have provided new insights into the mechanisms of how these modifications regulate the activity of various histone-modifying enzymes.
CURRENT OPINION IN STRUCTURAL BIOLOGY
(2023)
Article
Medicine, Research & Experimental
Zibo Zhao, Kaixiang Cao, Jun Watanabe, Cassandra N. Philips, Jacob M. Zeidner, Yukitomo Ishi, Qixuan Wang, Sarah R. Gold, Katherine Junkins, Elizabeth T. Bartom, Feng Yue, Navdeep S. Chandel, Rintaro Hashizume, Issam Ben-Sahra, Ali Shilatifard
Summary: Epigenetic alterations in chromatin-modifying enzymes have been shown to be associated with human diseases, including cancer. This study investigated the cellular dependencies and vulnerabilities resulting from loss of COMPASS family members MLL3 and MLL4. The results revealed that MLL3/4-deficient cells are dependent on purine synthesis pathways and are highly sensitive to the purine synthesis inhibitor lometrexol. Furthermore, tumors with MLL3 and/or MLL4 mutations also showed high sensitivity to lometrexol in both in vitro and in vivo experiments.
JOURNAL OF CLINICAL INVESTIGATION
(2023)
Article
Cell Biology
Carolina dos Santos Passos, Yun-Seok Choi, Christopher D. Snow, Tingting Yao, Robert E. Cohen
Summary: The study developed sensors for specifically ubiquitinated nucleosomes to monitor signaling from endogenous DNA damage, distinguishing the roles of 53BP1 and BARD1 in this histone posttranslational modification.
JOURNAL OF CELL BIOLOGY
(2021)
Article
Multidisciplinary Sciences
Yuqiu Wang, Yangyang Fan, De Fan, Yubo Zhang, Xiaoli Zhou, Ruikai Zhang, Yao Wang, Yujie Sun, Wei Zhang, Yuehui He, Xing Wang Deng, Danmeng Zhu
Summary: In this study, a plant-specific component of the DREAM complex called BTE1 was identified. It was found that the DREAM complex containing BTE1 acts against the COMPASS-like complex to repress transcription and occupancy of H3K4me3 at target genes. BTE1 was shown to interact with WDR5A, inhibiting its chromatin binding and transcription elongation on DREAM target genes.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Biochemistry & Molecular Biology
Wen Yang, Peter L. Hsu, Fan Yang, Jae-Eun Song, Gabriele Varani
NUCLEIC ACIDS RESEARCH
(2018)
Article
Multidisciplinary Sciences
Eric M. Janezic, Dorathy-Ann Harris, Diana Dinh, Kyung-Soon Lee, Aaron Stewart, Thomas R. Hinds, Peter L. Hsu, Ning Zheng, Chris Hague
SCIENTIFIC REPORTS
(2019)
Article
Biochemistry & Molecular Biology
Daohua Jiang, Hui Shi, Lige Tonggu, Tamer M. Gamal El-Din, Michael J. Lenaeus, Yan Zhao, Craig Yoshioka, Ning Zheng, William A. Catterall
Article
Biochemistry & Molecular Biology
Hui Wang, Hui Shi, Malini Rajan, Elizabeth R. Canarie, Seoyeon Hong, Daniele Simoneschi, Michele Pagano, Matthew F. Bush, Stefan Stoll, Elizabeth A. Leibold, Ning Zheng
Article
Biology
Jennifer L. Fribourgh, Ashutosh Srivastava, Colby R. Sandate, Alicia K. Michael, Peter L. Hsu, Christin Rakers, Leslee T. Nguyen, Megan R. Torgrimson, Gian Carlo G. Parico, Sarvind Tripathi, Ning Zheng, Gabriel C. Lander, Tsuyoshi Hirota, Florence Tama, Carrie L. Partch
Article
Biochemistry & Molecular Biology
Abhinav Dhall, Patrick M. M. Shelton, Aurore M-F Delachat, Calvin J. A. Leonen, Beat Fierz, Champak Chatterjee
Article
Biochemistry & Molecular Biology
Erika L. Buckle, Janani Sampath, Nina Michael, Samuel D. Whedon, Calvin J. A. Leonen, Jim Pfaendtner, Gary P. Drobny, Champak Chatterjee
Article
Multidisciplinary Sciences
Wei Wang, John Withers, Heng Li, Paul J. Zwack, Domnita-Valeria Rusnac, Hui Shi, Lijing Liu, Shunping Yan, Thomas R. Hinds, Mikelos Guttman, Xinnian Dong, Ning Zheng
Article
Biochemical Research Methods
Yue Liu, Congcong Ma, Calvin J. A. Leonen, Champak Chatterjee, Gabriela Novakova, Ales Marek, Frantisek Turecek
Summary: The study of gas-phase riboguanosine radicals with a fixed charge provides insights into the mechanism of radical generation, which is crucial for exploring reactions and processes related to nucleic acids.
JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY
(2021)
Article
Multidisciplinary Sciences
Ashleigh Shannon, Veronique Fattorini, Bhawna Sama, Barbara Selisko, Mikael Feracci, Camille Falcou, Pierre Gauffre, Priscila El Kazzi, Adrien Delpal, Etienne Decroly, Karine Alvarez, Cecilia Eydoux, Jean-Claude Guillemot, Adel Moussa, Steven S. Good, Paolo La Colla, Kai Lin, Jean-Pierre Sommadossi, Yingxiao Zhu, Xiaodong Yan, Hui Shi, Francois Ferron, Bruno Canard
Summary: AT-527 is a promising candidate drug that targets the SARS-CoV-2 replication machinery by inhibiting the RNA-dependent RNA polymerase.
NATURE COMMUNICATIONS
(2022)
Article
Biology
Calvin Jon A. Leonen, Miho Shimada, Caroline E. Weller, Tomoyoshi Nakadai, Peter L. Hsu, Elizabeth L. Tyson, Arpit Mishra, Patrick M. M. Shelton, Martin Sadilek, R. David Hawkins, Ning Zheng, Robert G. Roeder, Champak Chatterjee
Summary: Post-translational modification of histones by the small ubiquitin-like modifier protein SUMO plays a key role in gene silencing, centromeric localization, and DNA repair in eukaryotes. The study demonstrated that H4K12su inhibits acetylation of H4 tail and methylation of H3K4 associated with transcription activation, as well as transcription mediated by RNA polymerase II. Cell-based assays confirmed the negative crosstalk between histone sumoylation and acetylation/methylation in human cells.