期刊
MOLECULAR CELL
卷 77, 期 6, 页码 1279-+出版社
CELL PRESS
DOI: 10.1016/j.molcel.2020.01.019
关键词
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资金
- ARC fellowships
- Fondation pour la Recherche Medicale [SPE201801005113]
- European Research Council under the Horizon 2020 Program (ERC) [260822]
- European Research Council (ERC) [260822] Funding Source: European Research Council (ERC)
Cohesin, a member of the SMC complex family, holds sister chromatids together but also shapes chromosomes by promoting the formation of long-range intra-chromatid loops, a process proposed to be mediated by DNA loop extrusion. Here we describe the roles of three cohesin partners, Pds5, Wpl1, and Eco1, in loop formation along either unre-plicated or mitotic Saccharomyces cerevisiae chromosomes. Pds5 limits the size of DNA loops via two different pathways: the canonical Wpl1-mediated releasing activity and an Eco1-dependent mechanism. In the absence of Pds5, the main barrier to DNA loop expansion appears to be the centromere. Our data also show that Ecol acetyl-transferase inhibits the translocase activity that powers loop formation and contributes to the positioning of loops through a mechanism that is distinguishable from its role in cohesion establishment. This study reveals that the mechanisms regulating cohesin-dependent chromatin loops are conserved among eukaryotes while promoting different functions.
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