期刊
MOLECULAR AND CELLULAR ENDOCRINOLOGY
卷 499, 期 -, 页码 -出版社
ELSEVIER IRELAND LTD
DOI: 10.1016/j.mce.2019.110593
关键词
Anti-Mullerian hormone; CRISPR/Cas9; Folliculogenesis arrest; Ovary hypertrophy; Fertility
资金
- National Natural Science Foundation of China [31630082, 31861123001, 31872556, 31772830]
- National Key Research and Development Program of China [2018YFD0900202, 2018YFD0901201]
- Chongqing Science and Technology Commission [cstc2017jcyjAX0138, cstc2017shms-xdny80018, cstc2018jscx-mszd0380]
- Yunnan Science and Technology project [2018IB019]
- Fundamental Research Funds for the Central Universities [XDJK2018B026, XDJK2018D019]
- Chongqing Postgraduate Research Innovation Project [CYS18075]
In the present study, Amh was found to be abundantly expressed in the granulosa cells of the primary growth follicles, and Amhr2 in the granulosa cells, oogonia and phase I oocytes in tilapia by immunohistochemistry. In addition, Amh and Amhr2 were also found to be expressed in the brain and pituitary. Heterozygous mutation of either amh or amhr2 resulted in increased primary growth follicles and decreased fertility, and homozygous mutation resulted in hypertrophic ovaries with significantly increased primary follicles and failed transition from primary to vitellogenic follicles. Expression of gnrh3 in the brain, fsh and lh in the pituitary and serum E2 concentration were significantly decreased in both mutants. Significantly increased apoptosis of follicle cells was observed in both mutants. However, administration of E2 failed to rescue the folliculogenesis defects of the mutants. Our results suggested that Amh acts in a dose-dependent manner by binding Amhr2 in tilapia.
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