期刊
MICROBIOLOGY
卷 88, 期 6, 页码 671-680出版社
MAIK NAUKA/INTERPERIODICA/SPRINGER
DOI: 10.1134/S0026261719060110
关键词
PCR; primers; 16S rRNA gene; thermophilic microbial communities; high-throughput sequ-encing
类别
资金
- Russian Science Foundation [17-74-30025]
- Ministry of Science and Higher Education of the Russian Federation
- Russian Science Foundation [17-74-30025] Funding Source: Russian Science Foundation
Thermophilic microorganisms are of special interest for phylogenetics and research in prokaryotic evolution, since many of them belong to deep branches of the tree of life. For this reason a number of broadly used universal primer systems for the 16S rRNA gene eliminate certain groups of thermophilic prokaryotes from their detection spectra. In the present work, the known 16S rRNA gene primer systems were analyzed in order to determine their ability to reveal members of deep phylogenetic lineages containing thermophilic microorganisms. It was shown that application of most of the published primer systems could result in elimination of certain groups of thermophilic prokaryotes. In silico analysis of existing primer systems was used to select the primer system for the V3-V4 region of the 16S rRNA gene, which minimized elimination of thermophilic prokaryotic groups. Comparison of the proposed system with the previously published ones was carried out using high-throughput sequencing. Statistical analysis of the sequencing results based on the Shannon and Chao1 indexes revealed high efficiency of the proposed system for analysis of microbial communities of Kamchatka hot springs.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据