Review
Biotechnology & Applied Microbiology
Yong Sun Moon, Sajid Ali
Summary: Plant growth-promoting rhizobacteria (PGPR) colonize the plant rhizosphere and play a crucial role in promoting plant growth and mitigating abiotic stress. ACC deaminase-producing PGPR have been extensively used for improving crop growth under various stress conditions. However, the mechanism of ACC acquisition by ACC deaminase-producing bacteria is not well understood.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2022)
Review
Soil Science
M. Murali, H. G. Gowtham, S. Brijesh Singh, N. Shilpa, M. Aiyaz, S. R. Niranjana, K. N. Amruthesh
Summary: In agriculture, eco-friendly management practices are needed to enhance food security while avoiding harmful chemicals, with PGPR being considered as a potential alternative for plant growth improvement. ACC deaminase producing PGPR can help balance plant ethylene levels under abiotic stresses, showing promise as an environmental remediation approach. The selection of potential PGPR strain is critical, and plant responses to environmental conditions vary depending on various factors.
APPLIED SOIL ECOLOGY
(2021)
Article
Horticulture
Aruna Kumari Andy, Vishnu D. Rajput, Marina Burachevskaya, Vinod Singh Gour
Summary: Naturally available plant growth-promoting rhizobacteria (PGPR) have the ability to decrease ethylene levels and enhance abiotic stress tolerance in plants by processing plant-borne ACC through ACC deaminase enzymes. In this study, two rhizobacterial strains, Bacillus cereus and B. haynesii, were isolated from Vigna mungo and Phaseolus vulgaris and identified using 16S rDNA sequencing. The strains showed high ACC deaminase activity, exopolysaccharide yield, and indole acetic acid production, indicating their potential in improving drought tolerance and crop yield. Bacillus cereus also exhibited tolerance to As, Ba, and Ni, suggesting its suitability as a biofertilizer in fields affected by these metals.
Review
Microbiology
Mohammad Shahid, Udai B. B. Singh, Mohammad Saghir Khan, Prakash Singh, Ratan Kumar, Raj Narian Singh, Arun Kumar, Harsh V. V. Singh
Summary: Growth and productivity of crop plants worldwide are often adversely affected by stresses, both biotic and abiotic. Ethylene production in plants is induced by most stresses, which is detrimental to their growth and survival. Therefore, managing ethylene production in plants is becoming an attractive option for countering the negative effects on crop yield and productivity.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Microbiology
Rodrigo Mattos Silva Galeano, Fernanda Maria de Russo Godoy, Lais Mayara Melo Dure, Paulo Ivan Fernandes-Junior, Jose Ivo Baldani, Gecele Matos Paggi, Fabiana Fonseca Zanoelo, Marivaine Silva Brasil
Summary: The study isolated bacterial strains from ironstone outcrops in Brazil, with Bacillus cereus VBE23 demonstrating higher activity and tolerance under drought conditions, showing potential as a beneficial strategy for promoting plant growth.
CURRENT MICROBIOLOGY
(2021)
Article
Plant Sciences
Urooj Haroon, Maria Khizar, Fiza Liaquat, Musrat Ali, Mahnoor Akbar, Kinza Tahir, Syeda Saira Batool, Asif Kamal, Hassan Javed Chaudhary, Muhammad Farooq Hussain Munis
Summary: This study evaluated the growth-promoting characteristics of three bacterial strains in wheat plants under salinity stress. The results showed that these rhizobacteria positively influenced the physiology, biochemistry, and antioxidant enzymatic activities of the plants, mitigating the adverse effects of salinity stress. Additionally, inoculation of these bacteria increased the expression of stress tolerance genes and can potentially improve the plant's ability to tolerate salinity stress.
JOURNAL OF PLANT GROWTH REGULATION
(2022)
Article
Plant Sciences
Chaitanya Kumar Jha, Priyanka Sharma, Arpit Shukla, Paritosh Parmar, Rohit Patel, Dweipayan Goswami, Meenu Saraf
Summary: Ethylene has dual effects in plants, being harmful and beneficial, while microbes help reduce ethylene levels in plants by producing ACC deaminase, thereby promoting plant growth.
PHYSIOLOGICAL AND MOLECULAR PLANT PATHOLOGY
(2021)
Article
Plant Sciences
Anmol Gupta, Smita Rai, Ambreen Bano, Swati Sharma, Manoj Kumar, Reem Binsuwaidan, Mohammad Suhail Khan, Tarun Kumar Upadhyay, Nawaf Alshammari, Mohd Saeed, Neelam Pathak
Summary: This study found that two bacterial isolates, GKP KS2_7 (Pseudomonas aeruginosa) and MBD 133 (Bacillus subtilis), have ACC deaminase activity, which can inhibit the overproduction of ethylene and mitigate the negative effects of salinity stress on Pisum sativum through promoting various plant growth-promoting traits.
Article
Horticulture
Yingdan Yuan, Mengting Zu, Lanping Sun, Jiajia Zuo, Jun Tao
Summary: Plant growth-promoting rhizobacteria (PGPR) strains were isolated from the rhizosphere soil of Paeonia lactiflora cultivars and found to have diverse growth-promoting abilities and mechanisms. Inoculating with these strains significantly enhanced the growth and development of Paeonia lactiflora and Arabidopsis thaliana.
SCIENTIA HORTICULTURAE
(2022)
Article
Soil Science
Morgan McMillan, Cynthia M. Kallenbach, Joann K. Whalen
Summary: Soybean can enhance its tolerance to heat and water deficit by associating with rhizobacteria that reduce ethylene production. This study found that organic fertilizer and rhizobacteria with ACC deaminase activity can increase soybean tolerance to heat and water deficit by decreasing ethylene production and improving plant biomass.
APPLIED SOIL ECOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Pooja Singh, Shiv Shanker Pandey, Basant Kumar Dubey, Rashmi Raj, Deepti Barnawal, Ashish Chandran, Laiq Ur Rahman
Summary: The study demonstrated that transgenic Geranium plants showed significant increases in plant height, biomass, leaf: stem ratio, and oil quantity compared to wild-type plants, as well as enhanced tolerance to salt and drought stress. Analysis of various parameters revealed that transgenic Geranium lines exhibited decreased ACC content under stress conditions, leading to increased ethylene production and improved response to salt and drought stress.
PLANT CELL TISSUE AND ORGAN CULTURE
(2021)
Article
Plant Sciences
Lerato Nephali, Venessa Moodley, Lizelle Piater, Paul Steenkamp, Nombuso Buthelezi, Ian Dubery, Karl Burgess, Johan Huyser, Fidele Tugizimana
Summary: The study reveals the global metabolic landscape of maize leaves in response to a microbial biostimulant, highlighting key metabolic signatures such as increased TCA intermediates, altered amino acid levels, and changes in phenolics and lipids. These metabolic reconfigurations gravitate towards growth-promotion and defense preconditioning, contributing to enhanced drought resilience in maize plants.
FRONTIERS IN PLANT SCIENCE
(2021)
Review
Plant Sciences
Maha Chieb, Emma W. Gachomo
Summary: Climate change exacerbates the negative effects of abiotic stresses, such as drought, on plant growth and productivity. Plant colonization by plant growth-promoting rhizobacteria (PGPR) can enhance plant drought tolerance and improve plant growth and productivity.
Article
Microbiology
M. Murali, S. Brijesh Singh, H. G. Gowtham, N. Shilpa, Melvin Prasad, Mohammed Aiyaz, K. N. Amruthesh
Summary: Rhizobacteria from pearl millet with ACC deaminase-producing capabilities, such as Bacillus amyloliquefaciens MMR04, show promise in alleviating drought stress by enhancing seed germination, seedling vigor, and plant antioxidant system. These bacteria also exhibit potential for drought stress management through promoting plant growth and enhancing gene expression related to stress response and antioxidative activities.
MICROBIOLOGICAL RESEARCH
(2021)
Article
Plant Sciences
Shikha Gupta, Sangeeta Pandey, Vashista Kotra, Atul Kumar
Summary: The consortium inoculation with strains R1 and R4 modified the root system to boost seedling growth, increase the zinc content of French bean pods, and reduce salinity stress.
Article
Biochemistry & Molecular Biology
Efficient N. Ncube, Kgama Mathiba, Lucia H. Steenkamp, Ian A. Dubery
Summary: In this study, the filamentous yeast Hyphozyma roseoniger was used for biocatalytic conversion of sclareol to ambradiol, a key intermediate in the perfume industry. Metabolite profiling revealed the biochemical steps involved in this conversion process, showing a decrease in sclareol concentration and an increase in ambradiol concentration over time. Although unidentified peaks were observed in gas chromatography profiles, no new intermediates were discovered in the sclareol to ambradiol conversion pathway.
BIOCATALYSIS AND BIOTRANSFORMATION
(2022)
Article
Plant Sciences
Dylan R. Zeiss, Paul A. Steenkamp, Lizelle A. Piater, Ian A. Dubery
Summary: The study discovered that Ralstonia solanacearum-derived cold shock protein csp22 can elicit an immune response in tomato plants and investigated its effects on the plant's metabolome. The results show that csp22 pre-treatment can enhance tomato's resistance against R. solanacearum infection, contributing to the understanding of plant immune system operation at an integrative level.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Chemistry, Physical
Efficient N. Ncube, Paul A. Steenkamp, Chris W. van der Westhuyzen, Lucia H. Steenkamp, Ian A. Dubery
Summary: This study investigates the biocatalytic conversion of sclareol to ambradiol using whole-cell biotransformation by the yeast Hyphozyma roseoniger. Metabolomics tools, including NMR spectroscopy and LC-MS, were used to identify and quantify the intermediates involved in this bioconversion. The study highlights the transformative capabilities of H. roseoniger as a biocatalyst and its potential utility in the fragrance industry.
Article
Biochemistry & Molecular Biology
Manamele D. Mashabela, Lizelle A. Piater, Paul A. Steenkamp, Ian A. Dubery, Fidele Tugizimana, Msizi I. Mhlongo
Summary: This study employed an untargeted metabolomic approach to analyze metabolites of wheat cultivars resistant or susceptible to Puccinia striiformis f. sp. tritici (Pst) and Aluminium (Al3+) toxicity. A total of 100 metabolites were identified, and significant variations in metabolite concentrations were observed among different wheat cultivars. The findings provide insights into the genetic background and resistance mechanisms of wheat varieties against Pst and Al3+, demonstrating the potential applications of metabolomics in chemotaxonomic classification, phenotyping, plant breeding, and crop improvement.
Article
Biochemistry & Molecular Biology
Benedict C. Offor, Msizi I. Mhlongo, Paul A. Steenkamp, Ian A. Dubery, Lizelle A. Piater
Summary: Plants perceive pathogenic threats through pattern recognition receptors that recognize microbe-associated molecular patterns. The study shows that LPS-binding protein and bactericidal/permeability-increasing protein can perceive LPS and trigger defense responses. Metabolite analysis reveals that LPS can reprogram Arabidopsis metabolism and the accumulation of metabolites is influenced by different mutants and LPS chemotypes.
Article
Biochemistry & Molecular Biology
Msizi Mhlongo, Lizelle A. Piater, Ian A. Dubery
Summary: The rhizosphere microbiome plays a crucial role in plant health, and the volatile organic compounds (VOCs) secreted by plant-beneficial rhizobacteria (PGPR) have potential as eco-friendly substitutes for agrochemicals. In this study, the VOCs secreted by four PGPR strains were analyzed, revealing species-specific and strain-specific compounds that could be used as biomarkers for classification and agricultural selection.
Article
Plant Sciences
Thabiso E. Letseka, Ntjana J. Sepheka, Ian A. Dubery, Mosotho J. George
Summary: This study reports a simple, rapid, and robust technique called headspace bubble-in-drop microextraction (BID-SPME) for screening essential oils in oil-bearing plants. The method only requires a small amount of plant material and can extract at least 80% of the oil constituents. The extracted compounds can be easily annotated using gas chromatography-mass spectrometry.
Article
Agronomy
Francina L. Bopape, Chrizelle W. Beukes, Kopotsa Katlego, Ahmed I. Hassen, Emma T. Steenkamp, Eastonce T. Gwata
Summary: Pigeonpea is an important grain legume with low productivity in South Africa. This study characterized indigenous rhizobial strains and tested their nodulation abilities on local and improved genotypes of pigeonpea. The results showed that the rhizobia mainly belonged to Rhizobium and Bradyrhizobium and were able to nodulate both genotypes. However, the locally sourced landrace performed better. Further screening and field trials are needed to identify elite nitrogen fixing rhizobial strains for enhancing pigeonpea production in South Africa.
Article
Biochemistry & Molecular Biology
Claude Y. Hamany Y. Djande, Paul A. A. Steenkamp, Lizelle A. A. Piater, Fidele Tugizimana, Ian A. A. Dubery
Summary: Designing innovative biological crop protection strategies using eco-friendly alternatives to conventional biocidal agrochemicals is important. This study aimed to investigate the metabolic reprogramming in barley plants after treatment with three proposed dichlorinated inducers of acquired resistance. The results showed changes in the levels of both primary and secondary metabolites, including the activation of the phenylpropanoid pathway. Furthermore, the study highlighted the role of dichlorinated small molecules as inducers of plant immunity. This report provides valuable insights for metabolomics-guided plant improvement programs.
Review
Plant Sciences
Chanel J. Pretorius, Ian A. Dubery
Summary: Avenanthramides are phytoalexins produced in oat plants in response to pathogen attack. These compounds have multifunctional properties as plant defense substances, antimicrobial agents, and antioxidants, and also exhibit medicinal and pharmaceutical uses important for human health.
Article
Plant Sciences
Nikita da Camara, Ian A. Dubery, Lizelle A. Piater
Summary: Proteins play a crucial role in regulating innate immune response in plants under biotic or abiotic stresses. Isonitrosoacetophenone (INAP), an unusual stress metabolite, can induce plant defense responses. Transcriptomic and metabolomic studies of INAP-treated plant systems have provided insight into its defense-inducing and priming capabilities. In this study, a proteomic approach was used to examine time-dependent responses to INAP in Nicotiana tabacum cell suspensions. Proteome analysis revealed changes in defense-related proteins, as well as proteins involved in biosynthesis, transport, DNA transcription, metabolism, energy, translation, signaling, and response regulation. These findings highlight the importance of proteomic changes in priming induced by INAP treatment.
Article
Plant Sciences
Babra Moyo, Nikita Tawanda Tavengwa, Ian Dubery, Ntakadzeni Edwin Madala
Summary: In this study, the chemical interactions between V. combreticola and two of its host plants were investigated using UHPLC-q-TOF-MS profiling and molecular networking. The phytochemistry of V. combreticola was found to be independent and distinct from that of the host plants, and a unique chemistry of phenolic acid esterification to quinic acid was observed.
JOURNAL OF PLANT INTERACTIONS
(2023)
Article
Biochemistry & Molecular Biology
Claude Y. Hamany Djande, Fidele Tugizimana, Paul A. Steenkamp, Lizelle A. Piater, Ian A. Dubery
Summary: (English Summary:)
This study investigates the biochemical processes involved in the response of barley plants pre-treated with 3,5-dichloroanthranilic acid (3,5-DCAA) to Pyrenophora teres f. teres (Ptt). The results show that 3,5-DCAA delays the onset and reduces the severity of symptoms caused by Ptt. Metabolomic analyses reveal potential markers of the primed and naive plant responses to Ptt, including organic acids, amino acids, and hydroxycinnamic acids.
Article
Microbiology
Ahmed Idris Hassen, Langutani Sanger Khambani, Rian Pierneef, David A. Baltrus
Summary: The genome of Pseudomonas monsensis strain SARCC-3054, confirmed as a potential plant growth-promoting rhizobacteria, was sequenced and found to contain several plant beneficial genes and proteins.
MICROBIOLOGY RESOURCE ANNOUNCEMENTS
(2023)
Article
Biotechnology & Applied Microbiology
Langutani Sanger Khambani, Ahmed Idris Hassen, Karl Rumbold
Summary: This study characterizes rhizobia strains isolated from root nodules of indigenous and exotic legumes in South Africa and other countries. The strains were screened for their ability to tolerate abiotic stresses and were found to be phylogenetically related to various genera. This study provides baseline information for selecting rhizobia as inoculants under extreme environmental conditions in South Africa.
LETTERS IN APPLIED MICROBIOLOGY
(2023)
Article
Microbiology
Vera Analise Schommer, Mateus Torres Nazari, Flavia Melara, Julia Catiane Arenhart Braun, Alan Rempel, Lara Franco dos Santos, Valdecir Ferrari, Luciane Maria Colla, Aline Dettmer, Jeferson Steffanello Piccin
Summary: This review introduces the techniques and mechanisms of bacteria immobilization on biochar, as well as its applications in bioremediation and agriculture. The immobilization characteristics of biochar depend on pyrolysis methods, raw materials, and properties of biochar. Scanning electron microscope and colony forming unit analysis are commonly used to verify the immobilization efficiency. Applying biochar-immobilized bacteria can improve soil quality, plant growth, and crop yield.
MICROBIOLOGICAL RESEARCH
(2024)
Article
Microbiology
Manoj Kumar Solanki, Naveen Chandra Joshi, Prashant Kumar Singh, Sandeep Kumar Singh, Gustavo Santoyo, Lucas Carvalho Basilio de Azevedo, Ajay Kumar
Summary: The rhizosphere microbiome plays a crucial role in plant growth and health. Rhizosphere engineering can be used to modify the microbiome and improve plant productivity and resilience.
MICROBIOLOGICAL RESEARCH
(2024)
Article
Microbiology
Xin Cao, Xing-Wang Cheng, Yin-Ying Liu, Hong-Wei Dai, Ren-You Gan
Summary: Maintaining oral health is crucial for overall well-being. Imbalances in oral microorganisms can lead to various infectious diseases. Exploring alternatives for prevention and management of oral infections is important due to limitations of current treatments. Natural products offer potential as reliable, effective, and affordable therapeutic options for oral infectious diseases.
MICROBIOLOGICAL RESEARCH
(2024)
Article
Microbiology
Jose David Flores-Felix, Ana Carolina Goncalves, Sara Meirinho, Ana Raquel Nunes, Gilberto Alves, Cristina Garcia-Viguera, Diego A. Moreno, Luis R. Silva
Summary: The application of bacterial biofortifiers is increasing and has been shown to improve the nutritional characteristics of blueberries in Portugal.
MICROBIOLOGICAL RESEARCH
(2024)
Review
Microbiology
Asma Sani, Wan-Qi Qin, Jia-Yi Li, Yi-Fan Liu, Lei Zhou, Shi-Zhong Yang, Bo-Zhong Mu
Summary: This article summarizes the structural diversity of microbial lipopeptide biosurfactants and their applications as biocontrol agents in plants. Lipopeptides have low toxicity and wide range of applications, making them important in the pesticide industry.
MICROBIOLOGICAL RESEARCH
(2024)
Article
Microbiology
Xiao-Yu Sun, Jie Deng, Chenhui Zhang, Sin-Yee Fung, Kam-Leung Siu, Ying-Ying Cheng, Liumei Ye, Jiaoxia Qin, Ke Wang, Jiu-Xin Qu, Wenying Gao, Fuxiang Wang, Dong-Yan Jin, Liang Yang
Summary: In this study, a novel c-di-GMP binding protein SodA that regulates oxidative stress tolerance in S. maltophilia was identified. The enzyme activity of SodA was shown to be directly modulated by c-di-GMP through various in vivo and in vitro experiments.
MICROBIOLOGICAL RESEARCH
(2024)