期刊
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
卷 79, 期 6, 页码 912-918出版社
TAYLOR & FRANCIS LTD
DOI: 10.1080/09168451.2014.1002449
关键词
amyloid; edible vaccine; oxidative stress; 4-hydroxynonenal; Alzheimer's disease
类别
资金
- Ministry of Health, Labor and Welfare, Japan [23-5, 26-8]
- Comprehensive Research on Disability Health and Welfare, the Ministry of Health, Labor and Welfare, Japan
According to the amyloid hypothesis, amyloid beta accumulates in brains with Alzheimer's disease (AD) and triggers cell death and memory deficit. Previously, we developed a rice A beta vaccine expressing A beta, which reduced brain A beta levels in the Tg2576 mouse model of familial AD. We used senescence-accelerated SAMP8 mice as a model of sporadic AD and investigated the relationship between A beta and oxidative stress. Insoluble A beta and 4-hydroxynonenal (4-HNE) levels tended to be reduced in SAMP8 mice-fed the rice A beta vaccine. We attempted to clarify the relationship between oxidative stress and A beta in vitro. Addition of A beta peptide to the culture medium resulted in an increase in 4-HNE levels in SH-SY5Y cells. Tg2576 mice, which express large amounts of A beta in their brain, also exhibited increased 4-HNE levels; this increase was inhibited by the A beta vaccine. These results indicate that A beta induces oxidative stress in cultured cells and in the mouse brain.
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