期刊
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
卷 79, 期 11, 页码 1867-1875出版社
TAYLOR & FRANCIS LTD
DOI: 10.1080/09168451.2015.1061419
关键词
Small heat shock protein; slaughter age; poultry; skeletal muscle; age-related protein
类别
资金
- Ministry of Education, Culture, Sports, Science and Technology of Japan [23700832, 26350889]
International trading markets of meat require the animal's age information to prevent cross-contamination of ineligible meat products. Individual livestock age is either evaluated from physiological features or verified by breeding history. However, it remains impossible to perform age verification on meat when a suspicion of error occurred in the importing country. To investigate an age-related protein in skeletal muscle of livestock, we compared protein expression among chicken pectoralis major of different ages. Results indicated that the level of expression of chicken HSPB1, one of the small heat shock proteins, was increased in aged muscles. On the other hand, other heat shock proteins, heat shock factors, and myosin heavy chain isoform did not change the expression levels in aged chicken muscle. In addition, we identified that alpha B-crystallin interacted with HSPB1 in aged chicken muscle. These results suggest that HSPB1 protein forms complexes with alpha B-crystallin in aged chicken muscle and suppose to become the candidate of age-related bio-marker for verifying the age of chicken meat.
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