期刊
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
卷 80, 期 4, 页码 801-810出版社
TAYLOR & FRANCIS LTD
DOI: 10.1080/09168451.2015.1116924
关键词
olive; Escherichia coli; antimicrobial; growth inhibition; kinetic parameters
类别
资金
- COST ACTION [FA1202 BacFoodNet]
- Spanish Ministry of Economy and Competitiveness
This study focus on the main factors that affect the antimicrobial capacity of hydroxytyrosol, including the concentration (200, 400, and 1000g/mL), target strains, and the culture media (nutrient-rich and less-rich culture media). The potential HT degradation was also evaluated by HPLC-PAD. Kinetic parameters from growth curves showed that HT concentrations produced a doses-dependent shift when compared to the untreated control. In most of the cases, the highest tested dose (1000g/mL) was needed to inhibit growth of the selected strains. However, all the strains were able to grow even at the highest HT dose when cultivated in nutrient-rich culture media. It was observed that HT concentrations were reduced by about 15%, except for Escherichia coli 533 and 679 in Muller Hinton broth, where HT was reduced up to 35%. The results showed a limited antimicrobial activity, contrary to information previously published in some research papers.
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