期刊
JOURNAL OF PROTEOME RESEARCH
卷 19, 期 2, 页码 973-983出版社
AMER CHEMICAL SOC
DOI: 10.1021/acs.jproteome.9b00686
关键词
FFPE; tissue; immunohistochemistry; sample preparation; S-Trap; clinical proteomics
资金
- US National Institutes of Health (NIH) [T32GM008275, TL1TR001880, R01-GM110174, R01-A1118891, P01-CA196539]
- Leukemia and Lymphoma Society
- University of Pennsylvania Department of Pathology
- University of Pennsylvania Sarcoma Research Program
- NATIONAL CANCER INSTITUTE [P01CA196539] Funding Source: NIH RePORTER
Massive formalin-fixed, paraffin-embedded (FFPE) tissue archives exist worldwide, representing an invaluable resource for clinical proteomics research. However, current protocols for FFPE proteomics lack standardization, efficiency, reproducibility, and scalability. Here we present high-yield protein extraction and recovery by direct solubilization (HYPERsol), an optimized workflow using ultrasonication and S-Trap sample processing that enables proteome coverage and quantification from FFPE samples comparable to that achieved from flash-frozen tissue (average R = 0.936). When applied to archival samples, HYPERsol resulted in high-quality data from FFPE specimens in storage for up to 17 years, and may enable the discovery of new immunohistochemical markers.
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