期刊
JOURNAL OF MOLECULAR MICROBIOLOGY AND BIOTECHNOLOGY
卷 29, 期 1-6, 页码 66-73出版社
KARGER
DOI: 10.1159/000505371
关键词
Enteromorphapolysaccharides; Vibriospecies; CAZymes; Bioenergy
资金
- National Natural Science Foundation of China [41476150]
- Key Project of Higher Education of Guangdong Province [2016KZDXM011]
- Science and Technology Planning Project of Guangdong Province [2017A020217004]
In recent years, large-scale outbreaks of the green algaEnteromorpha proliferain China's offshore waters have posed a serious threat. This study aimed to improveEnteromorphapolysaccharide (EP) enzymatic sugar production using the hydrolase system ofVibriosp. H11, an EP-utilizing microbial strain. Strain H11 was found to contain 711 carbohydrate-related genes, and 259 genes belong to glycoside hydrolases that have the potential to hydrolyze EP. To maximize the capability of strain H11 to hydrolyze EP, both the culture medium and the composition were optimized. Response surface methodology analysis showed that maximal enzymatic production from strain H11 was 8.43 U/mL after 26-h incubation. When 50 g/L of EP were treated with crude H11 enzyme, the concentration of fermentation sugars increased by 36.12%. Under these conditions, the hydrolysates were capable of generating 3,217 mL/L of biogas and 6.74 g/L of biosolvents, with increases of 28.17 and 7.29%, respectively, compared to controls. The combined application of the H11 enzymatic system and anaerobic fermentation has the potential to improve the comprehensive application of EP.
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