Article
Medicine, General & Internal
Tadayoshi Ikebe, Hitoshi Otsuka, Kazuki Chiba, Yu Kazawa, Takahiro Yamaguchi, Rumi Okuno, Yoshimi Date, Mari Sasaki, Junko Isobe, Makoto Ohnishi, Yukihiro Akeda
Summary: This study identified csrS/csrR and srrG gene mutations that are associated with increased virulence gene expression in STSS-SDSE isolates. Strains carrying these mutations exhibited enhanced pathogenicity in mice. The higher frequency of mutations observed in STSS-SDSE isolates highlights their importance in STSS.
Article
Infectious Diseases
Guillem Lopez de Egea, Aida Gonzalez-Diaz, Gerard Guedon, Julie Lao, Damaris Berbel, Antonio Casabella, Jose Maria Marimon, Emilia Cercenado, Lucia Fernandez-Delgado, Helene Chiapello, Thomas Lacroix, Maria Angeles Dominguez, Nathalie Leblond-Bourget, Carmen Ardanuy
Summary: In this study, we investigated the genes and mobile genetic elements associated with tetracycline resistance in Streptococcus dysgalactiae subsp. equisimilis (SDSE). Out of 84 SDSE isolates, 28.5% were tetracycline resistant and carried tet(M), tet(W), or both tet(L) and tet(W) genes. The tet(M) gene was found in ICEs of the Tn916-family and a new IME. The new IME is a major cause of tetracycline resistance in invasive SDSE in our settings.
Article
Infectious Diseases
Viivi Nevanlinna, Reetta Huttunen, Janne Aittoniemi, Tiina Luukkaala, Sari Rantala
Summary: This study aimed to investigate the risk factors associated with S. dysgalactiae subsp. equisimilis bacteremia. Using the Finhealth study data in Finland, it was found that obesity, diabetes, and coronary artery disease were associated with an increased risk of S. dysgalactiae subsp. equisimilis bacteremia.
BMC INFECTIOUS DISEASES
(2023)
Article
Microbiology
Cinthia Alves-Barroco, Joao Caco, Catarina Roma-Rodrigues, Alexandra R. Fernandes, Ricardo Bexiga, Manuela Oliveira, Lelia Chambel, Rogerio Tenreiro, Rosario Mato, Ilda Santos-Sanches
Summary: The study suggests that there is a niche expansion for Streptococcus dysgalactiae subsp. dysgalactiae (SDSD) into human infections, possibly due to the acquisition of virulence genes. Genetic relationships between human SDSD, SDSE, and bovine SDSD were analyzed, showing closer relationships between human strains. The presence of the CRISPR/Cas IIA system among bovine SDSD isolates and the maintenance of virulence genes over time indicate potential activities and adaptations within the subspecies.
FRONTIERS IN MICROBIOLOGY
(2021)
Review
Infectious Diseases
Chen-Hong He, Shu-Fan Feng, Shu-Xin Chen, Deng-Ke Han, Tian-Rong He, Jian-Wei Cao, Hui-Qiang Mai
Summary: This is a case of human infection with SDSD, which is believed to be limited to animals. The patient experienced muscle pain and weakness, followed by sore throat, headache, and fever. After treatment, the patient's condition greatly improved and fully recovered within a month.
BMC INFECTIOUS DISEASES
(2023)
Article
Nutrition & Dietetics
Mohammad Shaokat Ali, Shamima Ahmed, Shino Takeuchi, Takayuki Wada, Eriko Kage-Nakadai
Summary: Lactococcus lactis subsp. lactis enhances locomotion in Caenorhabditis elegans and exerts its effects through modulation of specific transcription factors.
Article
Microbiology
Haruka Ishihara, Kohei Ogura, Van An Nguyen, Tohru Miyohi-Akiyama, Shigefumi Okamoto, Norihiko Takemoto
Summary: This study identified the complete genomic sequences of Lancefield group A SDSE strains, revealing a group-specific conserved region that can be targeted for differentiation through PCR. The growth of GA-SDSE was suppressed in the presence of other SDSE and S. pyogenes, potentially explaining the low frequency of isolation of GA-SDSE strains.
JOURNAL OF MEDICAL MICROBIOLOGY
(2021)
Article
Agriculture, Dairy & Animal Science
Anne Elisabeth Reetz, Etienne Aubry, Kinga Teske, Andreas Ochs, Lennard Epping, Torsten Semmler, Antina Luebke-Becker, Marcus Fulde, Lars Mundhenk
Summary: Movement disorders in rhinoceroses can be caused by lesions affecting the feet and interdigital space. This case study focuses on a 3-year-old male Greater one-horned rhinoceros that developed a severe movement disorder of the right hind limb and eventually died. The pathological analysis revealed a retroperitoneal abscess and chronic thrombosis of the right iliac artery with the presence of Streptococcus dysgalactiae subspecies equisimilis. The same bacteria was also found in the vaginal swab of the dam. This suggests that the list of differential diagnoses for lameness in rhinoceroses should consider causes beyond extremities.
Article
Microbiology
Andreas Itzek, Victoria Weissbach, David Meintrup, Beate Riess, Mark van der Linden, Stefan Borgmann
Summary: SDSE is an important beta-hemolytic pathogen, and this study reveals an increasing infection burden for the German population, mainly driven by a mutation-driven outbreak of the stG62647 strain. Men are more susceptible to infection, particularly those with the stG62647 strain, while women with non-stG62647 strains are younger. Increasing age is a general risk factor for invasive SDSE infections.
Review
Infectious Diseases
Keishin Sunagawa, Takuya Shirafuji, Guodong Sun, Rina Arai, Hiroki Azuma, Tohru Miyoshi-Akiyama, Harutaka Katano
Summary: This is the first case report of intra-familial transmission of Streptococcus dysgalactiae subsp. equisimilis.
JOURNAL OF INFECTION AND CHEMOTHERAPY
(2022)
Article
Veterinary Sciences
Carla Paola Bustos, Gabriela Retamar, Romina Leiva, Sara Frosth, Ana Ivanissevich, Maria Eugenia Demarchi, Saoirse Walsh, Lars Frykberg, Bengt Guss, Maria Mesplet, Andrew Waller
Summary: This study investigated the genotypic and phenotypic properties of Streptococcus dysgalactiae subsp. equisimilis (Sde) strain related to equine neonatal mastitis. It identified a novel genotype and horse-specific Sde strain as the causative agent of this condition.
JOURNAL OF EQUINE VETERINARY SCIENCE
(2023)
Article
Biochemistry & Molecular Biology
Sara Taylor, Deonne Walther, Deepani D. Fernando, Pearl Swe-Kay, Katja Fischer
Summary: This study investigates the antimicrobial potential of current and emerging scabicides and finds that both abametapir and manuka oil have antimicrobial properties against common scabies-associated bacteria. The current scabicide ivermectin also shows some antimicrobial activity.
Article
Infectious Diseases
Viivi Nevanlinna, Reetta Huttunen, Janne Aittoniemi, Tiina Luukkaala, Sari Rantala
Summary: This study investigated the incidence, seasonal pattern, clinical manifestations, and recurrence of Streptococcus dysgalactiae subspecies equisimilis (SDSE) bacteremia. A total of 217 episodes (involving 211 patients) were analyzed, with an average annual incidence rate of 16.9/100,000 inhabitants. The majority of episodes (33%) occurred during the summer, and bacteremic cellulitis was more common in the summer compared to other seasons. Cellulitis was the most common clinical manifestation of SDSE bacteremia.
EUROPEAN JOURNAL OF CLINICAL MICROBIOLOGY & INFECTIOUS DISEASES
(2023)
Article
Agriculture, Dairy & Animal Science
Miroslav Dinic, Stefan Jakovljevic, Nikola Popovic, Dusan Radojevic, Katarina Veljovic, Natasa Golic, Amarela Terzic-Vidojevic
Summary: This study investigated the probiotic features of two strains (S. thermophilus BGKMJ1-36 and L. bulgaricus BGVLJ1-21) isolated from artisanal sour milk and yogurt, and demonstrated their ability to upregulate autophagy-related genes and immune defense genes in Caenorhabditis elegans. The study also revealed that yogurt prepared with these strains is rich in vitamin B2 and dominant amino acids known for their pro-longevity properties. These findings highlight the potential of these starter cultures and yogurt as fermented foods with added-value properties.
JOURNAL OF DAIRY SCIENCE
(2023)
Editorial Material
Biochemistry & Molecular Biology
Patrick J. Hu
Summary: The study in C. elegans model showed that a conserved insulin-like signaling pathway plays an important role in shaping the phylogenetic composition of the gut microbiome.
Article
Multidisciplinary Sciences
Bruno Penna, Marcella B. Silva, Andre E. R. Soares, Ana T. R. Vasconcelos, Mariana S. Ramundo, Fabienne A. Ferreira, Maria C. Silva-Carvalho, Viviane S. de Sousa, Renata F. Rabello, Paula T. Bandeira, Viviane S. de Souza, Paul J. Planet, Olney Vieira-da-Motta, Ana M. N. Botelho, Agnes M. S. Figueiredo
Summary: MRSA is an important pathogen associated with various infections in humans, and research has shown that dogs can also be infected. Genotyping revealed different ST types of MRSA either colonizing or infecting dogs. Comparative genomics revealed similar virulence profiles for canine and human MRSA strains, suggesting they have similar infection potential.
SCIENTIFIC REPORTS
(2021)
Article
Microbiology
Caroline Lopes Martini, Amada Zambrana Coronado, Maria Celeste Nunes Melo, Clarice Neffa Gobbi, Ursula Santos Lopez, Marcos Correa de Mattos, Thais Tavares Amorim, Ana Maria Nunes Botelho, Ana Tereza Ribeiro Vasconcelos, Luiz Gonzaga Paula Almeida, Paul J. Planet, Russolina Benedeta Zingali, Agnes Marie Sa Figueiredo, Bernadete Teixeira Ferreira-Carvalho
Summary: This study has found that Streptococcus pyogenes can form antimicrobial persisters under certain conditions, possibly involving efflux pumps. The persistence phenomenon may be related to factors such as protein synthesis inhibition, cell growth impairment, and efflux pumps.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Chemistry, Medicinal
Francislene Juliana Martins, Fernanda Savacini Sagrillo, Rafaelle Josianne Vinturelle Medeiros, Alan Goncalves de Souza, Amanda Rodrigues Pinto Costa, Juliana Silva Novais, Leonardo Alves Miceli, Vinicius Campos, Agnes Marie Sa Figueiredo, Anna Claudia Cunha, Natalia Lidmar von Ranke, Murilo Lamim Bello, Barbara Abrahim-Vieira, Alessandra De Souza, Norman Ratcliffe, Fernanda da Costa Santos Boechat, Maria Cecilia Bastos Vieira de Souza, Carlos Rangel Rodrigues, Helena Carla Castro
Summary: This study evaluated the antibacterial activity of quinone-4-oxoquinoline derivatives and found that they exhibited broad-spectrum antibacterial activity, and in some cases were more effective than reference drugs. Additionally, these derivatives inhibited bacterial adhesion and some did not cause hemolysis. The study also suggested that the target of these derivatives may be a non-conventional hydrophobic allosteric binding pocket on the DNA gyrase enzyme.
CURRENT TOPICS IN MEDICINAL CHEMISTRY
(2022)
Article
Infectious Diseases
Marina Farrel Cortes, Camille Andre, Patricia Martins Simoes, Stephane Corvec, Jocelyne Caillon, Anne Tristan, Michele Bes, Francois Vandenesch, Agnes Marie Sa Figueiredo, Celine Dupieux, Frederic Laurent
Summary: Resistance to linezolid is a global concern, particularly in the treatment of multidrug-resistant staphylococcal and enterococcal infections. This study investigated linezolid-resistant staphylococcal infections in a French university hospital. The results showed the presence of a cfr-carrying plasmid that was efficiently spread between S. epidermidis and S. aureus, indicating the importance of plasmid-mediated resistance in the global dissemination of multidrug-resistant strains.
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY
(2022)
Article
Immunology
Cinthia Alves-Barroco, Ana Maria Nunes Botelho, Marco Antonio Americo, Sergio Eduardo Longo Fracalanzza, Antonio P. Alves de Matos, Marcia Aparecida Guimaraes, Bernadete Teixeira Ferreira-Carvalho, Agnes Marie Sa Figueiredo, Alexandra R. Fernandes
Summary: Streptococcus dysgalactiae subsp. dysgalactiae (SDSD) is a significant pathogen in bovine mastitis, causing inflammation and impacting the dairy industry. This study investigates the formation of biofilms by SDSD in a murine animal model and on human HEK cells. The results show that SDSD isolates can form biofilms on catheter surfaces in mice and cause damage to HEK cells.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Microbiology
Bruno Penna, Marcella B. Silva, Ana M. N. Botelho, Fabienne A. Ferreira, Mariana S. Ramundo, Maria C. Silva-Carvalho, Renata F. Rabello, Olney Vieira-da-Motta, Agnes M. S. Figueiredo
Summary: Staphylococcus pseudintermedius is a major bacterium associated with canine skin/soft tissue infections (SSTI), otitis externa, and surgical site infections. A methicillin-resistant strain called MRSP ST71, known as the European clone, has been found in dogs in Rio de Janeiro and may have spread to rural areas outside the city.
BRAZILIAN JOURNAL OF MICROBIOLOGY
(2022)
Article
Infectious Diseases
Alice Slotfeldt Viana, Ana Maria Nunes Botelho, Andries Feder, Ahmed Magdi Moustafa, Deborah Nascimento Santos Silva, Caroline Lopes Martini, Adriana Lucia Pires Ferreira, Maria Cicera Silva-Carvalho, Bernadete Teixeira Ferreira-Carvalho, Paul Joseph Planet, Agnes Marie Sa Figueiredo
Summary: This study identified a misclassification problem with SCCmec II in CC5 MRSA isolates, revealing that the insertion of TnSha1 carrying the fabI gene is the cause. The prevalence of TnSha1 may indicate strong selection for this phenotype and have implications for the molecular epidemiology of MRSA.
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY
(2022)
Article
Infectious Diseases
Deborah Nascimento Santos Silva, Cristiana Ossaille Beltrame, Ana Maria Nunes Botelho, Caroline Lopes Martini, Matheus Assis Cortes Esteves, Isabella Alvim Guedes, Laurent Emmanuel Dardenne, Agnes Marie Sa Figueiredo
Summary: Multidrug resistance is commonly acquired by transferring DNA from one bacterium to another. This study aims to understand the mechanisms that enhance the acquisition of foreign genes and the dynamics of their transmission between hosts in different environments. Genomic approaches were used to evaluate the enrichment of the S. aureus chromosome with resistance traits and analyze the evolutionary aspects of these acquisitions. The presence of anti-restriction gene homologs was strongly associated with patterns of multidrug resistance and promoted gene transfer. Understanding these mechanisms may help in controlling the spread of drug resistance.
Article
Microbiology
Maiana de Oliveira Cerqueira e Costa, Ana Paula Barbosa do Nascimento, Yasmmin Cortes Martins, Marcelo Trindade dos Santos, Agnes Marie de Sa Figueiredo, Ernesto Perez-Rueda, Marisa Fabiana Nicolas
Summary: This study reconstructed the gene regulatory network of the high biofilm-producing strain Bmb9393 of Staphylococcus aureus using literature-based and comparative genomics approaches. The network consists of 1,803 regulatory interactions between 64 transcription factors and 1,151 target genes. Additionally, the study identified a set of genes differentially expressed in the biofilm formation process, providing insights into the influence of different growth modes on regulatory systems.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Genetics & Heredity
Sabrina Di Gregorio, Jesus Vielma, Maria Sol Haim, Lucia Rago, Josefina Campos, Mihir Kekre, Monica Abrudan, Angela Famiglietti, Liliana Fernandez Canigia, Gabriela Rubinstein, Martha Helena von Specht, Melina Herrera, Carolina Aro, Marcelo Galas, Norah Balderrama Yarhui, Agnes Figueiredo, Nilton Lincopan, Miryan Falcon, Rosa Guillen, Teresa Camou, Gustavo Varela, David M. Aanensen, Silvia Argimon, Marta Mollerach, StaphNET-SA consortium
Summary: This article reports a genomic epidemiology study of Staphylococcus aureus in South America, which reveals the widespread antimicrobial resistance and genetic diversity of this pathogen in the region. The study also highlights the presence of high-risk clones and the need for continuous genomic surveillance.
MICROBIAL GENOMICS
(2023)
Article
Immunology
Alice Slotfeldt Viana, Ana Maria Nunes Botelho, Ahmed M. Moustafa, Craig L. K. Boge, Adriana Lucia Pires Ferreira, Maria Cicera da Silva Carvalho, Marcia Aparecida Guimaraes, Bruno de Souza Scramignon Costa, Marcos Correa de Mattos, Sabrina Pires Maciel, Juliana Echevarria-Lima, Apurva Narechania, Kelsey O'Brien, Chanelle Ryan, Jeffrey S. Gerber, Bernadete Teixeira Ferreira Carvalho, Agnes Marie Sa Figueiredo, Paul J. Planet
Summary: Researchers investigated 600 methicillin-resistant Staphylococcus aureus (MRSA) isolates collected in 51 hospitals in the Rio de Janeiro, Brazil, metropolitan area from 2014 to 2017, and identified an emerging multidrug-resistant MRSA sublineage named the Rio de Janeiro (RdJ) clone. The study also found that the RdJ clone, which began spreading in 2009, more effectively evaded immune function compared to other related isolates.
EMERGING INFECTIOUS DISEASES
(2021)
Article
Immunology
Marina Farrel Cortes, Ana Maria N. Botelho, Paula Terra Bandeira, William Mouton, Cedric Badiou, Michele Bes, Nicholas C. B. Lima, Andre Elias R. Soares, Rangel C. Souza, Luiz G. P. Almeida, Patricia Martins-Simoes, Ana T. R. Vasconcelos, Marisa F. Nicolas, Frederic Laurent, Paul J. Planet, Agnes M. S. Figueiredo
Summary: The ST1-SCCmecIV MRSA strains in hospitals in Brazil exhibit differences in genomic and phylogenetic characteristics compared to community-acquired strains in North America. These Brazilian strains may have originated from different MSSA strains independently acquiring the SCCmecIV cassette. The Brazilian MRSA strains have adapted to the hospital environment by reducing virulence and gaining survival abilities, possibly to balance the biological cost of retaining multiple antibiotic resistance genes.