Review
Physiology
Mian Muhammad Awais, Muhammad Shakeel, Jingchen Sun
Summary: miRNAs are small non-coding RNAs of about 22 nucleotides that have been reported to regulate gene expression at the posttranscriptional level, involved in various biological processes such as immunity, development, metabolism, and host-pathogen interactions. Both cellular and viral miRNAs may play vital roles in host-pathogen interactions, with viral infections altering host miRNA expression profiles.
FRONTIERS IN PHYSIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Su Lin, Hao Tong Yin, Zhi Meng Zhao, Zi Kang Chen, Xue MIng Zhou, Zheng Dong Zhang, Xi Jie Guo, Wei Guo Zhao, Ping Wu
Summary: In this study, it was found that lincRNA_XR209691.3, a long non-coding RNA, was significantly up-regulated in the silkworm fat body upon BmNPV infection. Further investigation revealed that lincRNA_XR209691.3 interacted with the host protein BmHSP70, promoting the replication and expression of BmNPV. These findings shed light on the role of lncRNAs in insect-pathogen interactions and provide new insights into the molecular mechanism of BmNPV infection.
INSECT MOLECULAR BIOLOGY
(2023)
Article
Zoology
Haotong Yin, Shaolun Zhang, Manman Shen, Zhengdong Zhang, Haoling Huang, Zhimeng Zhao, Xijie Guo, Ping Wu
Summary: By analyzing differentially expressed circRNAs, miRNAs, and mRNAs in the fat body of Bombyx mori in response to BmNPV infection, it was found that circRNA may play important roles in insect immune responses, with genes enriched in metabolic and signaling pathways. This suggests that circRNA could be involved in the immune responses of the fat body in silkworms and help understand the molecular mechanisms underlying interactions with pathogens.
JOURNAL OF INVERTEBRATE PATHOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Su Lin, Shao Lun Zhang, Hao Tong Yin, Zhi Meng Zhao, Zi Kang Chen, Man Man Shen, Zheng Dong Zhang, Xi Jie Guo, Ping Wu
Summary: This study found that the expression of Lnc_209997 was down-regulated in the silkworm fat body infected with Bombyx mori nucleopolyhedrosis virus (BmNPV). Inhibition of Lnc_209997 promoted BmNPV replication, while enhancing its expression inhibited the proliferation of BmNPV. Additionally, miR-275-5p was up-regulated in the infected silkworm fat body, and Lnc_209997 was found to interact with miR-275-5p. Over-expression of miR-275-5p facilitated BmNPV replication. These results suggest that BmNPV increases the expression of miR-275-5p by inhibiting cellular Lnc_209997 expression to promote its own proliferation.
INSECT MOLECULAR BIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Zhi-meng Zhao, Hao-tong Yin, Man-man Shen, Shao-lun Zhang, Zi-kang Chen, Tao Li, Zhen-dong Zhang, Wei-guo Zhao, Xi-jie Guo, Ping Wu
Summary: This study reveals the inhibitory effect of Geldanamycin (GA) on the proliferation of Bombyx mori nuclear polyhedrosis virus (BmNPV) and uncovers the regulatory network involving miRNAs. The findings enrich our understanding of the interaction between BmNPV and silkworms, and improve our knowledge on the molecular mechanism of HSP90 inhibitors in BmNPV proliferation.
ARCHIVES OF INSECT BIOCHEMISTRY AND PHYSIOLOGY
(2022)
Review
Microbiology
Yi-Xuan Fan, Vivian Andoh, Liang Chen
Summary: This paper reviewed the current research on selecting BmNPV-resistant genes, proteins, and metabolites using transcriptomics, proteomics, metabolomics, and related techniques. The regulatory networks between viruses and hosts have been gradually clarified with the discovery of ncRNAs, such as miRNA, lncRNA, and circRNA in cells. The findings provide references for resistant strategies in the silkworm to BmNPV.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Weiwei Zhou, Jindie Hong, Jinying Han, Fuchuan Cai, Qi Tang, Qian Yu, Guohui Li, Shangshang Ma, Xiaoyong Liu, Shuhao Huo, Keping Chen, Feifei Zhu
Summary: In this study, the role of glycosaminoglycan modifications, including heparan sulfate (HS) and chondroitin sulfate (CS), in Bombyx mori nuclear polyhedrosis virus (BmNPV) infection was investigated. The results showed that HS and CS are important attachment factors for BmNPV and targeting them can effectively inhibit viral infection.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Virology
Guanping Chen, Yuedong Li, Xiangshuo Kong, Shudi Zhao, Jiale Li, Xiaofeng Wu
Summary: This study found that the expression of Bombyx mori HEXIM1 (BmHEXIM1) was significantly upregulated after BmNPV infection. By suppressing BmRelish-driven immune responses, BmHEXIM1 assisted in the immune escape of BmNPV. This research provides new insights into the mechanisms of immune escape of BmNPV and theoretical guidance for the breeding of BmNPV-resistant silkworm varieties.
Article
Biochemistry & Molecular Biology
Qian Zhang, Yun-Fei Wu, Peng Chen, Tai-Hang Liu, Zhan-Qi Dong, Cheng Lu, Min-Hui Pan
Summary: Cdc37 is a molecular chaperone that plays an active role in the proliferation of BmNPV in silkworm cells. Studies have shown that Cdc37 interacts with Hsp90 to promote virus replication. Co-expression of Cdc37 and Hsp90 is more effective in enhancing virus proliferation than overexpression alone.
PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY
(2021)
Article
Entomology
Xiong Wang, Guangyu Ma, Feifei Ren, Mian Muhammad Awais, Jingchen Sun
Summary: Research has shown that Bombyx mori fatty acid binding protein 1 (BmFABP1) is expressed in silkmoth cells and in the tissues of silkmoth larvae, where it inhibits the replication of Bombyx mori nucleopolyhedrovirus (BmNPV). This study provides new insights into the potential antiviral role of BmFABP1 and its importance in breeding BmNPV-resistant transgenic silkmoths.
Article
Entomology
Qin Xiao, Zhan-Qi Dong, Yan Zhu, Qian Zhang, Xiu Yang, Miao Xiao, Peng Chen, Cheng Lu, Min-Hui Pan
Summary: Our study demonstrated that Bombyx mori nucleopolyhedrovirus (BmNPV) induces G2/M phase arrest in insect cells by downregulating BmCDK1 and BmCyclin B, essential for its replication. The interaction of BmNPV IAP1 with BmCDK1 was confirmed, suggesting a mechanism through which BmNPV impacts cell cycle progression. These findings enhance our understanding of virus-host interaction and provide a potential target gene linking apoptosis and the cell cycle.
Article
Agronomy
Xin Wang, Jun-Li Lv, Shuang Cheng, Zhi-Hao Su, Sheng Qin, Xia Sun, Xu-Dong Tang, Qiu-Ning Liu, Mu-Wang Li, Xue-Yang Wang
Summary: This study elucidates the important role of BmE74A in promoting the proliferation of BmNPV by directly interacting with the virus. The high expression of BmE74A in eggs, hemolymph, and midgut, as well as the differential expression in different resistant strains, confirms its significance in viral infection.
PEST MANAGEMENT SCIENCE
(2022)
Article
Fisheries
Xiu Shi, Yaxin Zhang, Tianchen Zhu, Nan Li, Sufei Sun, Min Zhu, Jun Pan, Zeen Shen, Xiaolong Hu, Xing Zhang, Chengliang Gong
Summary: The study utilized 16S rDNA sequencing and metabolomics to show significant alterations in gut microbiota and metabolites of silkworm midgut after BmNPV infection. Analysis revealed enrichment of specific pathways related to viral infection and metabolism, highlighting the relationship between digestive tract gut microbiota, metabolites, and host-virus interaction. These findings enhance understanding of viral infection links to midgut microbiota and metabolic activity in the silkworm model.
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
(2021)
Article
Immunology
Mengmeng Zhang, Shigang Fei, Junming Xia, Yeyuan Wang, Hongyun Wu, Xian Li, Yiyao Guo, Luc Swevers, Jingchen Sun, Min Feng
Summary: This study is the first to investigate the role of Sirtuins in insect-virus interactions. It found that silkworm Sirt2 and Sirt5 have antiviral functions against BmNPV, and silkworm Sirt5 enhances host antiviral response by activating the immune pathway mediated by Relish.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Fisheries
Xiaoli Kang, Yaping Wang, Wenjuan Liang, Xin Tang, Yan Zhang, Lingyan Wang, Ping Zhao, Zhongyan Lu
Summary: BmFoxO expression decreases after BmNPV infection in silkworms, affecting PEPCK expression. Overexpression of BmFoxO enhances antiviral resistance in silkworms by upregulating PEPCK.
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
(2021)