Article
Biochemistry & Molecular Biology
Saskia Schwarzer, Michael Radzieta, Slade O. Jensen, Matthew Malone
Summary: A topical desiccating wound agent containing specific ingredients was evaluated for its efficacy against biofilms produced by two bacterial strains, demonstrating complete eradication in different in vitro models.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Hanif Haidari, Richard Bright, Sanjay Garg, Krasimir Vasilev, Allison J. Cowin, Zlatko Kopecki
Summary: The development of highly potent ultrasmall AgNPs combined with a biocompatible hydrogel has shown promising results in eliminating mature biofilm infections in vivo and promoting wound healing. The treatment exhibited superior antibacterial efficacy and improved wound closure, re-epithelization, and collagen deposition compared to conventional silver-based treatments.
Review
Pharmacology & Pharmacy
Raj Kumar Thapa, Jong Oh Kim, Jeonghwan Kim
Summary: This review discusses conventional, current, and prospective anti-biofilm therapies for treating biofilm-based wound infections. While conventional strategies have limited efficacy, current treatments such as antimicrobial peptides and nanoparticles show promise. However, prospective strategies such as NADES and CRISPRi are required due to the prevalence of microbial resistance.
JOURNAL OF PHARMACEUTICAL INVESTIGATION
(2023)
Article
Biochemistry & Molecular Biology
Madelene A. Andersson, Lone Bruhn Madsen, Artur Schmidtchen, Manoj Puthia
Summary: This study introduces an ex vivo wound infection model using porcine skin and a burn wounding method, allowing for the efficacy evaluation of topical antimicrobial formulations. The results demonstrate compatibility with clinically relevant bacterial strains and confirm the effectiveness of antibacterial treatments, highlighting the formation of biofilms on the wound surface as well.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Chemistry, Multidisciplinary
Fan Zhao, Yajuan Su, Junying Wang, Svetlana Romanova, Dominick J. DiMaio, Jingwei Xie, Siwei Zhao
Summary: Biofilm infection in chronic wounds is common and delays wound healing. This paper describes a highly effective electrical antibiofilm treatment system that uses high-intensity current to debride biofilm and enhance antibiotic delivery. The system reduces bacterial count of MRSA biofilm-infected ex vivo skin wounds and demonstrates in vivo antibiofilm efficacy in a diabetic mouse-based wound infection model. This novel technology provides a quick, safe, and highly efficacious treatment for chronic wound biofilm infections.
ADVANCED MATERIALS
(2023)
Review
Dermatology
Kevin Woo, Caroline Dowsett, Ben Costa, Stephen Ebohon, Emma J. Woodmansey, Matthew Malone
Summary: The study summarizes the clinical effectiveness of cadexomer iodine in the treatment of chronic wounds, promoting wound bed preparation and healing process by removing barriers to healing.
INTERNATIONAL WOUND JOURNAL
(2021)
Article
Dermatology
Yanbing Zhang, Pengyu Zhang, Han Li, Haitao Chi, Nan Zheng, Xu Pan, Chuzhong Tang
Summary: We conducted a meta-analysis to evaluate the impact of topical vancomycin (TV) application on reducing sternal wound infections (SWIs) following cardiac surgery (CS). The analysis included 23,745 participants who underwent CS, with 8,730 using TV and 15,015 as controls. The results showed that TV significantly reduced the occurrence of SWIs and deep SWIs post-CS compared to the control group.
INTERNATIONAL WOUND JOURNAL
(2023)
Review
Biochemistry & Molecular Biology
Firas Diban, Silvia Di Lodovico, Paola Di Fermo, Simonetta D'Ercole, Sara D'Arcangelo, Mara Di Giulio, Luigina Cellini
Summary: Chronic wounds have detrimental effects on patients and healthcare systems. The search for effective compounds to treat these wounds has been ongoing, with a focus on combating microbial colonization by multidrug resistant pathogens. The development of an in vitro model, such as the Lubbock chronic wound biofilm model, has facilitated the study of wound poly-microbial biofilms and the evaluation of innovative treatment strategies.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Pharmacology & Pharmacy
Nan Wang, Feng Qi, Xiaolong He, Honglan Shi, David W. Anderson, Hao Li, Hongmin Sun
Summary: Antibiotic resistance is a global public health threat. A novel anti-virulence compound, CCG-211790, has potential to ameliorate human diseases by inhibiting virulence factors. Nanosuspensions with smaller particle sizes showed improved dissolution rate and oral bioavailability compared to traditional formulations.
Article
Pharmacology & Pharmacy
Alaa S. S. Eita, Amna M. A. Makky, Asem Anter, Islam A. A. Khalil
Summary: This study aims to improve the antifungal activity of atorvastatin (ATO) by encapsulating it in an emulsomes (EMLs) system, which enhances skin permeation. The optimized ATO-EMLs formula showed good antifungal activity and stability, with limited skin irritation.
Review
Agriculture, Dairy & Animal Science
Elin Jorgensen, Thomas Bjarnsholt, Stine Jacobsen
Summary: Delayed wound healing in equine limb wounds may be attributed to biofilm infections, which are difficult to detect with routine diagnostics. Treatment options include aggressive debridement and application of topical antimicrobial therapy. Biofilm presence in equine wounds requires advanced detection techniques for proper diagnosis and management.
Article
Chemistry, Multidisciplinary
Suhasini Mallick, Moupriya Nag, Dibyajit Lahiri, Soumya Pandit, Tanmay Sarkar, Siddhartha Pati, Nilesh Prakash Nirmal, Hisham Atan Edinur, Zulhisyam Abdul Kari, Muhammad Rajaei Ahmad Mohd Zain, Rina Rani Ray
Summary: The treatment of chronic wound infections involves the complex interaction of immunity and host processes, with the formation of biofilm contributing to antimicrobial resistance. Nanomaterials are being developed as a non-conventional antimicrobial approach to disrupt and remove biofilms, improving wound healing effectiveness. Extensive research is focusing on various nanoplatforms, including metallic and nonmetallic nanoparticles, to enhance the treatment of chronic wounds.
Article
Infectious Diseases
Matthew Regulski, Matthew F. F. Myntti, Garth A. A. James
Summary: This study aimed to evaluate the anti-biofilm capabilities of eight commercially available wound care products using in vitro assays for biofilms. The products included dressings with different formats for ionic silver and non-silver-based products. The anti-biofilm efficacy was measured on Staphylococcus aureus and Pseudomonas aeruginosa mixed-species biofilms. Four of the eight wound care products tested were effective in inhibiting the growth of new biofilms. BDWG achieved the highest reduction in mature biofilm growth. Evaluating both biofilm prevention and mature biofilm disruption is crucial for understanding the anti-biofilm efficacy of wound care products.
Article
Dermatology
Jianghe Zhang, Yan Yan, Yujie Li, Chengcheng Shen, Yiming Zhang
Summary: Benzalkonium bromide (BB) showed the best wound infection control efficiency while delaying wound healing, and exhibiting higher cytotoxicity and inducing more cell apoptosis compared to other antiseptics like PVP-I and CHG. BB also induced more cell apoptosis and ROS production at higher levels, possibly leading to delayed wound healing.
INTERNATIONAL WOUND JOURNAL
(2021)
Article
Dermatology
Jennifer Cooley, Noor Obaidi, Victoria Diaz, Kristin Anselmo, Elof Eriksson, Anders H. Carlsson, Rodney K. Chan, Kristo Nuutila
Summary: This study evaluated the safety and efficacy of the platform wound device (PWD) as a protective dressing and a delivery system for topical antibiotics. The delivery of gentamicin via the PWD was found to be safe and effective.
INTERNATIONAL WOUND JOURNAL
(2023)
Article
Orthopedics
Hanna H. Sorensen, Robert A. Magnussen, Alex C. DiBartola, Noah T. Mallory, Alan S. Litsky, Paul Stoodley, Steven D. Swinehart, Robert A. Duerr, Christopher C. Kaeding, David C. Flanigan
Summary: This study aimed to determine the impact of bacterial inoculation and length of exposure on the mechanical integrity of soft tissue tendon grafts. The presence of Staphylococcus epidermidis increased the elasticity and decreased the ultimate tensile strength of human cadaveric tendons, with a more significant effect observed with increasing bioburden.
JOURNAL OF ORTHOPAEDIC RESEARCH
(2023)
Article
Biotechnology & Applied Microbiology
Mengfei Li, Yanina Nahum, Karel Matous, Paul Stoodley, Robert Nerenberg
Summary: This study investigates the effects of biofilm mechanical heterogeneity on the results obtained by rheometry. Both experiments and simulations show that the biofilm properties determined by rheometry are strongly influenced by the weakest portion of the biofilm. The findings suggest caution should be exercised when interpreting rheometry data, particularly when the mechanical properties are stratified.
BIOTECHNOLOGY AND BIOENGINEERING
(2023)
Review
Microbiology
Hans-Curt Flemming, Eric D. van Hullebusch, Thomas R. Neu, Per H. Nielsen, Thomas Seviour, Paul Stoodley, Jost Wingender, Stefan Wuertz
Summary: In this review, the authors revisit the understanding of the biofilm matrix and discuss the diversity of its components and the novel aspects of their functional interactions. The biofilm matrix plays a crucial role in stabilizing microbial cells and fulfilling various functions. It is dynamic and influenced by physico-chemical and biological interactions as well as environmental conditions.
NATURE REVIEWS MICROBIOLOGY
(2023)
Article
Immunology
Jacob R. Brooks, Douglas J. Chonko, Matthew Pigott, Anne C. Sullivan, Kelly Moore, Paul Stoodley
Summary: This study aimed to investigate whether certain components and their surface features are more prone to biofilm formation in primary total joint arthroplasty. It was found that biofilm predominantly formed on the non-articulating surfaces between components and on ridges and edges. This has important implications for intraoperative debridement, retention choices, and implant design.
Article
Biotechnology & Applied Microbiology
Erum Razvi, Gregory B. Whitfield, Courtney Reichhardt, Julia E. Dreifus, Alexandra R. Willis, Oxana B. Gluscencova, Erin S. Gloag, Tarek S. Awad, Jacquelyn D. Rich, Daniel Passos da Silva, Whitney Bond, Francois Le Mauff, Donald C. Sheppard, Benjamin D. Hatton, Paul Stoodley, Aaron W. Reinke, Gabrielle L. Boulianne, Daniel J. Wozniak, Joe J. Harrison, Matthew R. Parsek, P. Lynne Howell
Summary: Pel exopolysaccharide biosynthetic loci are widespread biofilm matrix determinants in bacteria. In Pseudomonas aeruginosa, the glycoside hydrolase activity of PelA decreases adherent biofilm biomass and generates the low molecular weight secreted form of the Pel exopolysaccharide. Secreted Pel contributes to the biomechanical properties of the biofilm and decreases the virulence of P. aeruginosa in Caenorhabditis elegans and Drosophila melanogaster.
NPJ BIOFILMS AND MICROBIOMES
(2023)
Article
Microbiology
Erin S. Gloag, Yalda Khosravi, James G. Masters, Daniel J. Wozniak, Carlo Amorin Daep, Paul Stoodley
Summary: Mechanical removal of dental plaque is augmented with therapeutic compounds to promote oral health. A dentifrice containing the ingredients zinc and arginine has shown efficacy at reducing dental plaque both in vitro and in vivo. However, how these active compounds interact together to facilitate dental plaque removal is unclear. Here, we used a combination of biophysical analyses and microscopy to demonstrate that combined treatment with zinc and arginine targets the matrix of dental plaque biofilms, which destabilized the mechanical integrity of these microbial communities, making them more susceptible to removal by shear forces.
MICROBIOLOGY SPECTRUM
(2023)
Article
Biochemistry & Molecular Biology
Farhana Parvin, Md. Arifur Rahman, Anand K. Deva, Karen Vickery, Honghua Hu
Summary: Staphylococcus aureus biofilms exhibit resistance to antibiotics and disinfectants, which is attributed to changes in the bacterial cell wall. Proteins involved in cell wall synthesis are upregulated in biofilms compared to planktonic organisms. Furthermore, the width of the cell wall and peptidoglycan production increase with biofilm culture duration and dehydration. Disinfectant tolerance is highest in dry surface biofilms, followed by hydrated biofilms, and lowest in planktonic bacteria, indicating that changes in the cell wall may contribute to the biocide resistance of S. aureus biofilms.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Microbiology
Amelia Staats, Peter W. W. Burback, Nadia N. N. Casillas-Ituarte, Daniel Li, Michaela R. R. Hostetler, Anne Sullivan, Alexander R. R. Horswill, Steven K. K. Lower, Paul Stoodley
Summary: The formation of a bacterial biofilm in the postsurgical joint environment significantly complicates the resolution of an infection. This study investigated the mechanisms of Staphylococcus aureus aggregation in synovial fluid and identified clumping factor A as the primary genetic determinant of aggregation. The study also found that the size and antibiotic tolerance of the aggregates formed under different conditions varied.
JOURNAL OF BACTERIOLOGY
(2023)
Article
Chemistry, Physical
Rui Han, Xi-Qiao Feng, Waldemar Vollmer, Paul Stoodley, Jinju Chen
Summary: Bacteria adapt the mechanical properties of their cell envelope, including cell wall stiffness, turgor, and cell wall tension and deformation, to grow and survive in harsh environments. This study quantified the mechanical properties and turgor of Staphylococcus epidermidis using theoretical modelling and experimental approaches. The results showed that high osmolarity leads to a decrease in both cell wall stiffness and turgor, and that the turgor change is associated with a change in bacterial cell viscosity. The study also found that cell wall tension is higher in deionized water and decreases with an increase in osmolality.
JOURNAL OF COLLOID AND INTERFACE SCIENCE
(2023)
Article
Gastroenterology & Hepatology
Simone Vieira Toledo Guadagnin, Dayane de Melo Costa, Mariusa Gomes Borges Primo, Adriana Azevedo Silva, Lara Stefania Netto de Oliveira Leao-Vasconcelos, Karen Vickery, Anaclara Ferreira Veiga Tipple
Summary: This study aimed to determine bacterial contamination in flexible gastroscopes channels after storage in a cabinet without forced-air drying. The results showed that bacterial contamination increased with longer storage time.
JOURNAL OF GASTROENTEROLOGY AND HEPATOLOGY
(2023)
Review
Microbiology
Victor Choi, Jennifer L. Rohn, Paul Stoodley, Dario Carugo, Eleanor Stride
Summary: In this Review, emerging drug delivery strategies are discussed as a means to improve the clinical efficacy of antibiofilm agents in preventing and eliminating pathogenic biofilms. The potential benefits of these techniques include improved penetration of antimicrobials through biofilms, enhanced drug dispersal, and synergistic bactericidal effects. However, current limitations and future prospects for these therapies in the clinic are also highlighted.
NATURE REVIEWS MICROBIOLOGY
(2023)
Article
Multidisciplinary Sciences
Rajdeep Chakraborty, Charbel Darido, Arthur Chien, Aidan Tay, Karen Vickery, Honghua Hu, Fei Liu, Shoba Ranganathan
Summary: The role of Gipie in adenoid cystic carcinoma (ACC) and mucoepidermoid carcinoma (A-253) was evaluated in this study. Results showed that silencing Gipie led to morphological changes in the cells, increased apoptosis, decreased T regulatory cells, and increased activated NK cells.
SCIENTIFIC REPORTS
(2023)
Article
Microbiology
Kelly Moore, Anthony Li, Niraj Gupta, Tripti Thapa Gupta, Craig Delury, Sean S. Aiken, Phillip A. Laycock, Paul Stoodley
Summary: This in-vitro study found that high purity antibiotic-loaded calcium sulfate beads containing vancomycin and tobramycin in combination may be more effective in treating multispecies biofilm orthopedic infections than monotherapy alone.
Article
Chemistry, Physical
Alexandra A. Snowdon, Simon P. Dennington, Jennifer E. Longyear, Julian A. Wharton, Paul Stoodley
Summary: Marine biofilms on ship hulls increase frictional drag, which has economic and environmental consequences. The study aimed to characterise the rheological properties of ship-relevant marine biofilms. The results confirmed that surface treatment influences the structural and mechanical properties of marine biofilms, which could have implications for drag.
Article
Radiology, Nuclear Medicine & Medical Imaging
Monique Doran, Gemma Reemst, Kenny Ng, Courtney Shaw, Paul Stoodley